Clarification of vasospasm by a spatiotemporal nanoscale analysis of signal molecules.
| Project/Area Number |
20700359
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurophysiology and muscle physiology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
KAWAMICHI Hozumi Yamaguchi University, 大学院・医学系研究科, 助教 (80363042)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
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| Keywords | 平滑筋生理 / 可視化 / 生体分子 / リアルタイム / 血管攣縮 / 平滑筋 / 一分子 / ナノシステム |
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| Research Abstract |
Vasospasm is a Rho-kinase-mediated Ca^<2+>-independent abnormal contraction. However, its final step (= interaction of contractile proteins) was not determined yet. In this study, we developed a spatiotemporal nanoscale analysis of signal molecules, which were confirmed by mass spectrometry. Using this system, we found that even in the absence of Ca^<2+> smooth muscle myosin, whose regulatory light chains are phosphorylated by Rho-kinase, can show rapid sliding with actin at the maximum high speed.
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Report
(3 results)
Research Products
(21 results)
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[Journal Article] 日薬理誌2009
Author(s)
川道穂津美、岸博子、加治屋勝子、高田雄一、小林誠
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Journal Title
血管異常収縮の分子機構と分子標的治療薬の探索 133
Pages: 124-129
Related Report
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[Journal Article] Time Studies2008
Author(s)
Hozumi Kawamichi.
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Journal Title
Watching the motion pictures of signal molecules of the abnormal vascular contraction! Spatiotemporal nanoscale analysis of intracellular abnormality. 2
Pages: 45-64
Related Report
Peer Reviewed
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