Creation of artificial transcription factors for artificial gene circuits

• Project/Area Number: 20710165
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Living organism molecular science
• Research Institution: Kyoto University
• Principal Investigator: IMANISHI Miki Kyoto University, 化学研究所, 助教 (80362391)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 亜鉛フィンガー / 人工転写因子 / 遺伝子制御

Research Abstract

Artificial gene circuits is expected to provide a new way to understand various biological events controlled by feedback regulations. Towards the usage of such artificial gene circuits, artificial DNA binding proteins with various DNA binding properties, such as DNA binding affinity, selectivity, kinetics et al., would be important tools. So artificial zinc finger-type DNA binding proteins and transcription factors with various properties for DNA binding were created and examined the functions. Metal-responsive artificial DNA binding proteins and transcription factors were also successfully created.

Research Products

• [Journal Article] Positive and Negative Cooperativity of Modularly Assembled Zinc Fingers. (2009)
  • Author(s): Imanishi M, Nakamura A, Morisaki T, Futaki S
  • Journal Title: Biochem. Biophys. Res. Commun. 387 Pages: 440-443
  • NAID: 120002753564
  • Peer Reviewed
• [Journal Article] Cobalt(II)-Responsive DNA Binding of a GCN4-bZIP Protein Containing Cysteine Residues Functionalized with Iminodiacetic Acid. (2009)
  • Author(s): Azuma Y, Imanishi M, Yoshimura T, Kawabata T, Futaki S
  • Journal Title: Angew. Chem. Int. Ed. 48 Pages: 6853-6856
  • Peer Reviewed
• [Journal Article] Cobalt(II)-Responsive DNA Binding of a GCN4-bZIP Protein Containing Cysteine Residues Functionalized with Iminodiacetic Acid (2009)
  • Author(s): Azuma Y, Imanishi M, Yoshimura T, Kawabata T, Futaki S
  • Journal Title: Angew.Chem.Int.Ed. 48 Pages: 6853-6856
  • Peer Reviewed
• [Journal Article] Positive and Negative Cooperativity of Modularly Assembled Zinc Fingers (2009)
  • Author(s): Imanishi M, Nakamura A, Morisaki T, Futaki S
  • Journal Title: Biochem.Biophys.Res.Commun. 387 Pages: 440-443
  • NAID: 120002753564
  • Peer Reviewed
• [Journal Article] Rapid Transcriptional Activity in Vivo and Slow DNA Binding in Vitro by and Artificial Multi-Zinc Finger Protein. (2008)
  • Author(s): Morisaki T, Imanishi M, Futaki S, Sugiura Y
  • Journal Title: Biochemistry 47 Pages: 10171-10177
  • Peer Reviewed
• [Journal Article] Rapid Transcriptional Activity in Vivo and Slow DNA Binding in Vitro by an Artificial Multi-Zinc Finger Protein (2008)
  • Author(s): Morisaki, T., Imanishi, M., Futaki, S., and Sugiura, Y
  • Journal Title: Biochemistry 47 Pages: 10171-10177
  • Peer Reviewed
• [Presentation] 「生命現象の制御を目指した人工転写因子の創製 (2010)
  • Author(s): 今西未来
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山市
  • Year and Date: 2010-03-29
• [Presentation] 生命現象の制御を目指した人工転写因子の創製 (2010)
  • Author(s): 今西未来
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山市
  • Year and Date: 2010-03-29
• [Presentation] ゲノムレベルでの時計遺伝子プロモーター解析を可能にする亜鉛フィンガー型人工転写因子の創製 (2010)
  • Author(s): 中村篤史、今西未来、土居雅夫、岡村均、二木史朗
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山市
  • Year and Date: 2010-03-28
• [Presentation] 生命現象解明ツールとしてのジンクフィンガー型人工転写因子の創製 (2010)
  • Author(s): 今西未来
  • Organizer: 第12回生命化学研究会
  • Place of Presentation: あわら市
  • Year and Date: 2010-01-09
• [Presentation] 時計遺伝子プロモーター解析へ向けた亜鉛フィンガー型人工転写因子の創製 (2009)
  • Author(s): 中村篤史、今西未来、土居雅夫、岡村均、二木史朗
  • Organizer: 第6回アジア睡眠学会日本睡眠学会第34回定期学術集会第16回日本時間生物学会学術大会2009合同大会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-10-27
• [Presentation] 時計遺伝子プロモーター解析へ向けた亜鉛フィンガー型人工転写因子の創製 (2009)
  • Author(s): 中村篤史、今西未来、土居雅夫、岡村均、二木史朗
  • Organizer: 第6回アジア睡眠学会 日本睡眠学会第34回定期学術集会 第16回日本時間生物学会学術大会 2009合同大会
  • Place of Presentation: 大阪市
  • Year and Date: 2009-10-27
• [Presentation] Metal-Induced DNA-Binding Switch of bZIP Proteins Modified with Iminodiacetic Acid (Ida) (2009)
  • Author(s): Azuma Y, Imanishi M, Yoshimura T, Kawabata T, Futaki S
  • Organizer: The Fifth iCeMS International Symposium, Biomaterials at the Interface of Chemistry, Physics, and Biology
  • Place of Presentation: 京都市
  • Year and Date: 2009-07-27
• [Presentation] Creation of artificial zinc finger-type transcription factors and effects on the rhythmic expression of clock genes (2009)
  • Author(s): 今西未来、中村篤史、二木史朗
  • Organizer: 第19回金属の関与する生体関連シンポジウム(SRM 2009)
  • Place of Presentation: 吹田市
  • Year and Date: 2009-06-12
• [Presentation] Creation of artificial zinc finger-type transcription factors and effects on the rhythmic expression of clockgenes (2009)
  • Author(s): 今西未来、中村篤史、二木史朗
  • Organizer: 第19回金属の関与する生体関連シンポジウム(SRM2009)
  • Place of Presentation: 吹田市
  • Year and Date: 2009-06-12
• [Presentation] シスエレメントを標的とした人工ジンクフィンガー連結体のDNA結合性の解析 (2009)
  • Author(s): 今西未来、中村篤史、二木史朗
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 2009-05-22
• [Presentation] 細胞内におけるマルチ亜鉛フィンガータンパク質のDNA結合特性 (2009)
  • Author(s): 森崎達也、今西未来、二木史朗、杉浦幸雄
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 熊本市
  • Year and Date: 2009-05-22
• [Remarks] ホームページ等
  • URL: http://www.scl.kyoto-u.ac.jp/~bfdc/index.html
• [Patent(Industrial Property Rights)] 遺伝子発現調節タンパク質 (2010)
  • Inventor(s): 二木史朗、今西未来、能代大輔、中屋智博、森崎達也
  • Industrial Property Rights Holder: 二木史朗、今西未来、能代大輔、中屋智博、森崎達也
  • Filing Date: 2010-03-01
• [Patent(Industrial Property Rights)] 遺伝子発現調節タンパク質 (2010)
  • Inventor(s): 二木史朗、今西未来、能代大輔、中屋智博、森崎達也
  • Industrial Property Rights Holder: 二木史朗、今西未来、能代大輔、中屋智博、森崎達也
  • Industrial Property Number: 2010-044245
  • Filing Date: 2010-03-01