Development of a method for the synthesis of polymers containing ester bond (s) by genetic code expansion
| Project/Area Number |
20710177
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Living organism molecular science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
KOBAYASHI Takatsugu The Institute of Physical and Chemical Research, システム研究チーム, 特別研究員 (90446518)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 非天然型アミノ酸 / 翻訳 / tRNA / アミノアシルtRNA合成酵素 / タンパク質工学 / ポリエステル / 高分子化学 / 遺伝暗号 |
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| Research Abstract |
Highly homogenious polyester can be synthesized by genetic-information-based synthesis of polypeptide-like polymers. In this study, an aminoacyl-tRNA synthetase was altered for the incorporation of ester bond into the polymers synthesized on ribosome. As a result, the mutant enzymes, which efficiently recognize the building block of the ester bonds, were found. By using these enzymes, the in vitro translation system that synthesizes polymers containing two serially polymerized α-hydroxyacids, was successfully established.
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Report
(3 results)
Research Products
(4 results)
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[Journal Article]2009
Author(s)
小林隆嗣, 柳沢達男, 坂本健作, 横山茂之
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Journal Title
Journal of Molecular Biology Vol.385
Pages: 1352-1360
Related Report
Peer Reviewed
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