Molecular systems underlying the regulation of anaphase-promoting complex/cyclosome ubiquitin ligase activity

Research Project

Project/Area Number	20710178
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Living organism molecular science
Research Institution	The Institute of Physical and Chemical Research
Principal Investigator	SHOJI Shisako 独立行政法人理化学研究所, システム研究チーム, 研究員 (60321808)
Project Period (FY)	2008 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000) Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000) Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords	細胞周期 / ユビキチン化反応 / APC/サイクロソーム / タンパク質複合体 / Emi2 / 細胞分裂 / ユビキチン化制御 / ユビキチン化 / APC / サイクロソーム / タンパク質立体構造 / 細胞分裂停止因子 / 立体構造解析 / 構造変化 / ユビキチンリガーゼ
Research Abstract	Anaphase-promoting complex or cyclosome(APC/C) is a multi-subunit ubiquitin ligase that marks the target cell cycle regulators for proteasomal degradation. Emi2 was identified to be an essential component of the cytostatic factor, which antagonizes APC/C function in unfertilized vertebrate eggs. This study revealed the presence of the ubiquitin chain extension-interfering region within mouse Emi2.We propose a novel model for APC/C regulation, which differs from the previously reported mechanisms to block substrate recognition.