Improvement and application of Cryptosporidium detection with Florescent in situ Hybridization
| Project/Area Number |
20760366
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Anan National College of Technology |
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Principal Investigator |
HASHIMOTO Atsushi Anan National College of Technology, 生命環境学部, 准教授 (30332068)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Keywords | Cryptosporidium / FISH / クリプトスポリジウム / FISH法 / 水環境 / 指標細菌 / 遺伝子型 / 蛍光抗体染色 |
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| Research Abstract |
Fluorescence in situ hybridization (FISH) was applied for specific detection and distinction of Cryptosporidium oocysts by microscopic observation. High fluorescence was observed using the DIAG-CRYR1 probe targeting 18S rRNA partial sequences to distinguish Cryptosporidium spp from Cryptosporidium parvum HNJ-1 oocysts. Combining FISH with fluorescence antibody staining, DAPI, and differential interference contrast observation was valuable for easy differentiation of Cryptosporidium oocysts by microscopic observation. Determination of Cryptosporidium variability by FISH was conducted. Heat inactivated oocysts declined FISH stainability until 100 hours in both PBS and filtrated sewage.
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Report
(4 results)
Research Products
(6 results)
