Breeding of yeast for efficient bio-ethanol production
| Project/Area Number |
20760538
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kanazawa University |
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Principal Investigator |
NINOMIYA Kazuaki Kanazawa University, 環日本海域環境研究センター, 助教 (10379125)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | バイオエタノール / 酵母 / 酵素発現 / FACS / 転写因子 / 代謝進化工学 |
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| Research Abstract |
By using immunocytochemistry and fluorescent activated cell sorter (FACS), yeast population highly expressing cellulase on the cell surface was enriched from the mutant population prepared by irradiation with carbon ion beams (220 MeV^<12>C^<5+>, 100 Gy). The cellulase activity of selected yeasts was 5.2×10^<-3>U/OD unit, whereas cellulase activity of parental strain was 1.6×10^<-3>U/OD unit.
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Report
(3 results)
Research Products
(18 results)
