Anti-carcinogenic glucosinolate: Molecular mechanism of its synthesis and degradation

• Project/Area Number: 20770044
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Kyushu University (2010); Fukui Prefectural University (2008-2009)
• Principal Investigator: MARUYAMA Akiko (MARUYAMA NAKASHITA Akiko) 九州大学, 農学研究院, 准教授 (70342855)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 植物 / シグナル伝達 / 硫黄 / グルコシノレート / 機能未知遺伝子

Research Abstract

We identified gene X repress methionine-derived glucosinolate (mGSL) synthesis, by ａ reverse genetic study on the genes up-regulated by sulfur deficiency. Gene expression of X and the homologue X' were detected and up-regulated in every tissue in Arabidopsis. By analyzing the plants disrupted and over-expressed X and X', we revealed that mGSL accumulation can be controlled by modifying X and X' transcription.

Research Products

• [Presentation] 植物の硫酸イオン吸収・硫黄同化系制御機構の解明 (2011)
  • Author(s): 丸山明子
  • Organizer: 日本土壌肥料学会20011年度大会
  • Place of Presentation: つくば市(茨城県)
  • Year and Date: 2011-08-09
• [Presentation] Transcriptional regulation of genes involved in sulfur assimilation in plants: Understanding from the analysis of high affinity transporters. (2008)
  • Author(s): Akiko Maruyama-Nakashita
  • Organizer: China-Japan Plant Nutrition Workshop
  • Place of Presentation: 北京(中国)
• [Presentation] 硫酸イオン吸収の制御：転写制御機構の解析から． (2008)
  • Author(s): 丸山明子
  • Organizer: 日本土壌肥料学会2008年度大会
  • Place of Presentation: 名古屋(愛知県)
• [Presentation] Toward comprehensive understanding of regulatory network of sulfur metabolism. (2008)
  • Author(s): T. Fujiwara, A, Maruvama Nakashita, Y. Ide, M. Yokota Hirai
  • Organizer: The 7th International Workshop on Plant Sulfur Metabolism
  • Place of Presentation: ワルシャワ(ポーランド)
• [Remarks]
  • URL: http://bbsl.agr.kyushu-u.ac.jp/prweb2/b06/amaru/amn/youkoso.html
• [Patent(Industrial Property Rights)] メチオニン由来グルコシノレート生合成抑制遺伝子を利用した植物の作出方法 (2009)
  • Inventor(s): 丸山明子, 高橋秀樹, 斉藤和季
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Patent Publication Number: 2009-284799
  • Acquisition Date: 2009-12-10
• [Patent(Industrial Property Rights)] メチオニン由来グルコシノレート生合成抑制遺伝子を利用した植物の作出方法 (2008)
  • Inventor(s): 丸山明子, 高橋秀樹, 斉藤和季
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2008-140020
  • Filing Date: 2008-05-28
• [Patent(Industrial Property Rights)] 特許権 (2008)
  • Inventor(s): 丸山明子, 高橋秀樹, 斉藤和季
  • Industrial Property Rights Holder: 独立行政法人理化学研究所
  • Industrial Property Number: 2008-140020
  • Filing Date: 2008-05-28