Recognition of presequences of mitochondrial precursor proteins using dynamic equilibrium

• Project/Area Number: 20770087
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: SAITOH Takashi Kyushu University, デジタルメディシン・イニシアティブ, 助教 (00432914)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 分子認識及び相互作用 / 蛋白質 / 相互作用 / NMR / X線結晶構造解析 / ミトコンドリア / 構造生物学 / タンパク相互作用

Research Abstract

In previous study, we proposed that a dynamic equilibrium between the multiple bound states is the molecular mechanism of the broadly selective specificity of the Tom20 receptor towards the divergent mitochondrial presequences. In this study, we carried out X-ray crystal structure analysis and NMR spectroscopy experiments using new complexes between Tom20 and presequenses. We obtained further information supporting the dynamic recognition model.

Research Products

• [Journal Article] ミトコンドリアのプレ配列は動的平衡メカニズムにより認識される (2009)
  • Author(s): 齊藤貴士, 尾瀬農之, 神田大輔
  • Journal Title: 生物物理 vol.49(2009),No.5
  • NAID: 10025975892
  • Peer Reviewed
• [Journal Article] ミトコンドリアのプレ配列は動的平衡メカニズムにより認識される (2009)
  • Author(s): 齊藤貴士・尾瀬農之・神田大輔
  • Journal Title: 生物物理 49 Pages: 242-243
  • NAID: 10025975892
  • Peer Reviewed
• [Presentation] 構造生物学から見たミトコンドリアTom20による前駆蛋白質のプレ配列認識 (2009)
  • Author(s): 齊藤貴士, 宮崎佑介, 尾瀬農之, 神田大輔
  • Organizer: 第9回日 本蛋白質科学会年会
  • Place of Presentation: 熊本
  • Year and Date: 2009-05-21
• [Presentation] 構造生物学から見たミトコンドリアTom20による前駆蛋白質のプレ配列認識 (2009)
  • Author(s): 齊藤貴士・宮崎佑介、尾瀬農之、神田大輔
  • Organizer: 第9回日本蛋白質科学会年会
  • Place of Presentation: 態本
  • Year and Date: 2009-05-21
• [Presentation] Relaxation Analysis of the Tom20-Bound states of a Mitochondrial Presequence. (2008)
  • Author(s): Takashi Saitoh, Daisuke Kohda
  • Organizer: XXIIrd ICMRBS
  • Place of Presentation: USA
  • Year and Date: 2008-08-25
• [Presentation] Relaxation Analysis of the Tom20-Bound states of a Mitochondorial Presequence (2008)
  • Author(s): Saitoh, Tkakshi
  • Organizer: XXIII International Conference on Magnetic Resonance in Biological System
  • Place of Presentation: San-Diego
  • Year and Date: 2008-08-25
• [Presentation] 複数の結合様式を介したミトコンドリアTom20によるプレ配列の認識機構 (2008)
  • Author(s): 齊藤貴士、井倉真由美、尾瀬農之、前中勝実、神田大輔
  • Organizer: 第8回日本蛋白質科学会年会
  • Place of Presentation: 東京
  • Year and Date: 2008-06-11