| Project/Area Number |
20770110
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Fukuoka University |
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Principal Investigator |
MORI Masayuki Fukuoka University, 医学部, 講師 (80342640)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Keywords | 生理学 / シグナル伝達 / カルシウム / イメージング / 構造 / 一般生理 / 細胞内カルシウム / イオンチャネル / カルシウム結合蛋白質 / X線構造解析 / 興奮性細胞 / 蛍光エネルギー移動(FRET) |
|---|
| Research Abstract |
The overall aim of the proposed studies was to understand the mechanism of activation, inhibition and regulations of Ca channels by Calcium-binding protein Calmodulin (CaM). The primary focus was structural determination of the calcium free form apoCaM and Channel IQ domain complex. Until today, we have a couple of crystals, those will be needed to check reproducibility and their structure. For the imaging of Ca^<2+> and CaM- channel interaction, we have succeeded to develop new system combined with Fura-2 and FRET. By using this system we could establish Ca^<2+> titrative experiment in vivo with high fidelity. We hope that this approach will be often to use in the field wide range of the physiological science to reveal Ca^<2+> related molecular dynamics.
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