Elucidation of systems for mouse PGC specification regulated by pluripotent-associatedgene Oct3/4
| Project/Area Number |
20770170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | Tohoku University |
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Principal Investigator |
OKAMURA Daiji Tohoku University, 加齢医学研究所, 助教 (80393263)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生殖細胞 / 始原生殖細胞 / 遺伝子欠損マウス / 多能性 / 転写抑制 / マウス / 分化決定 / Oct3 / 4 / 転写抑制因子 / Rest / 相同組み替え / ES細胞 |
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| Research Abstract |
We had screened the genes were first detected their expression in just specified primordial germ cells, that resulted in identification of Rest/NRSF gene. So we generated knock-out mice for Rest gene. The results showed that the number of primordial germ cells at migrating stage in Rest mutant embryos significantly decreased, compared with that in wild embryos, it caused by apoptotic cell death, but not defect of proliferation rate of primordial germ cells.
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Report
(3 results)
Research Products
(6 results)
