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Establishment of transient RNAi methods in tree fruit and it's application on evaluation of genes regulating fruit ripening

Research Project

Project/Area Number 20780021
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Horticulture/Landscape architecture
Research InstitutionOkayama University

Principal Investigator

NAKANO Ryouhei  Okayama University, 大学院・自然科学研究科, 准教授 (70294444)

Co-Investigator(Renkei-kenkyūsha) KUBO Yasutaka  岡山大学, 大学院・自然科学研究科, 教授 (80167387)
USHIJIMA Koichiro  岡山大学, 大学院・自然科学研究科, 教授 (20379720)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsRNAサイレンシング / 果実 / 成熟 / RNAサインレンシング / hair-pin RNAi / 成熟制御因子 / MADS-Box転写因子 / GRAS / カンキツ / 着色関連遺伝子 / ブドウ
Research Abstract

Transient RNA silencing technique was suggested to be possible in developing grape berry. When tomato is used as model plant for screening of fruit ripening-associated genes, AP3, TM6, AGL1, GRAS were assumed to be involved in ripening. Involvements of these genes in ripening of grape berry were investigated using the transient RNA silencing technique, although their involvements were not clear. It is suggested that the transient RNA silencing technique would be useful for analysis of ripening-associated genes in grape in future and that deeper investigation of function of these genes for fruit ripening will provide new insight into the study for fruit physiology.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (7 results)

All 2011 2008 Other

All Presentation (6 results) Remarks (1 results)

  • [Presentation] Screening of ripening related transcription factors using tomato DNA macroarray, 1-MCP and ripening impaired mutants2011

    • Author(s)
      Erin Zui
    • Organizer
      園芸学会平成20年度秋季大会
    • Place of Presentation
      岡山市
    • Year and Date
      2011-03-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Screening of ripening related transcription factors using tomato DNA macroarray, 1-MCP and ripening impaired mutants2011

    • Author(s)
      Erin Zui
    • Organizer
      Joint International Symposium on Japanese Solanaceae/Cucurbitaceae Genomics Initiatives
    • Place of Presentation
      岡山市
    • Year and Date
      2011-03-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 一過的RNAサイレンシングを用いた果実および花における遺伝子機能の解析2008

    • Author(s)
      中野龍平
    • Organizer
      園芸学会平成20年度秋季大会
    • Place of Presentation
      津市
    • Year and Date
      2008-09-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Virus induced gene scilencing 法による成熟関連MADS-Box転写因子の探索2008

    • Author(s)
      久保拓也
    • Organizer
      園芸学会平成20年度秋季大会
    • Place of Presentation
      津市
    • Year and Date
      2008-09-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 一過的RNAサイレンシングを用いた果実および花における遺伝子機能の解析2008

    • Author(s)
      中野龍平
    • Organizer
      園芸学会平成20年度秋季大会
    • Place of Presentation
      三重大学
    • Year and Date
      2008-09-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Virus induced gene scilencing法による成熟関連MADS-Box転写因子の探索2008

    • Author(s)
      久保拓也
    • Organizer
      園芸学会平成20年度秋季大会
    • Place of Presentation
      三重大学
    • Year and Date
      2008-09-27
    • Related Report
      2008 Annual Research Report
  • [Remarks] 特になし

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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