Development of thermostable protein production system using the radioresistant bacterium

• Project/Area Number: 20780079
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Applied biochemistry
• Research Institution: Japan Atomic Energy Agency
• Principal Investigator: SATOH Katsuya Japan Atomic Energy Agency, 量子ビーム応用研究部門, 研究員 (90370402)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生物工学 / 遺伝子工学 / 放射線抵抗性細菌 / 高度好熱菌 / 耐熱性タンパク質 / タンパク質生産系 / シャトルベクター / プロモーター配列

Research Abstract

Some proteins originated from thermophile had difficulty with production by the conventional method using Escherichia coli as a host cell. In this study, to develop a novel system for thermostable protein production using the radioresistant bacterium, we searched a useful promoter sequence for gene expression and constructed a novel expression plasmid vector. As a result, the production of thermostable protein without insolubilization could be confirmed using a radioresistant bacterium as a host cell.

Research Products

• [Journal Article] Deinococcus aetherius sp. nov., isolated from the stratosphere (2010)
  • Author(s): Yinjie Yang, Takashi Itoh, Shin-ichi Yokobori, Haruo Shimada, Shiho Itahashi, Katsuya Satoh, Hirofumi Ohba, Issay Narumi, Akihiko Yamagishi
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology Vol.60,No.4 Pages: 776-779
  • Peer Reviewed
• [Journal Article] Deinococcus aetherius sp.nov., isolated from the stratosphere (2010)
  • Author(s): Yinjie Yang
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology 60 Pages: 776-779
  • Peer Reviewed
• [Journal Article] Deinococcus aerius sp. nov., isolated from the high atmosphere (2009)
  • Author(s): Yinjie Yang, Takashi Itoh, Shin-ichi Yokobori, Shiho Itahashi, Haruo Shimada, Katsuya Satoh, Hirofumi Ohba, Issay Narumi, Akihiko Yamagishi
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology Vol.59,No.8 Pages: 1862-1866
  • Peer Reviewed
• [Journal Article] Development of versatile shuttle vectors for Deinococcus grandis (2009)
  • Author(s): Katsuya Satoh, Zhenli Tu, Hirofumi Ohba, Issay Narumi
  • Journal Title: Plasmid Vol.62,No.1 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Issay Narumi, Identification of PprM: a modulator of the PprI-dependent DNA damage response in Deinococcus radiodurans (2009)
  • Author(s): Hirofumi Ohba, Katsuya Satoh, Haitham Sghaier, Tadashi Yanagisawa
  • Journal Title: Extremophiles Vol.13,No.3 Pages: 471-479
  • Peer Reviewed
• [Journal Article] Identification of PprM : a modulator of the PprI-dependent DNA damage response in Deinococcus radiodurans (2009)
  • Author(s): Hirofumi Ohba
  • Journal Title: Extremophiles 13 Pages: 471-479
  • Peer Reviewed
• [Journal Article] Development of versatile shuttle vectors for Deinococcus grandis (2009)
  • Author(s): Katsuya Satoh
  • Journal Title: Plasmid 62 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Deinococcus aerius sp.nov., isolated from the high atmosphere (2009)
  • Author(s): Yinjie Yang
  • Journal Title: International Journal of Systematic and Evolutionary Microbiology 59 Pages: 1862-1866
  • Peer Reviewed
• [Journal Article] 放射線抵抗性細菌の放射線耐性の謎に迫る (2008)
  • Author(s): 大庭寛史、佐藤勝也、鳴海一成
  • Journal Title: 放射線と産業 Vol.118 Pages: 50-53
• [Journal Article] 放射線抵抗性細菌の超放射線耐性の謎に迫る (2008)
  • Author(s): 大庭寛史
  • Journal Title: 放射線と産業 118 Pages: 50-53
• [Presentation] 放射線抵抗性細菌における新規遺伝子操作系の開発 (2009)
  • Author(s): 佐藤勝也
  • Organizer: 第10回極限環境微生物学会年会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-28
• [Presentation] 放射線抵抗性細菌由来DNA修復促進タンパク質PprAの構造機能解析 (2009)
  • Author(s): 山田貢
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-21
• [Presentation] 放射線抵抗性細菌のプラスミドを用いた新規遺伝子操作系の開発 (2009)
  • Author(s): 佐藤勝也
  • Organizer: 第4回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎
  • Year and Date: 2009-10-09
• [Presentation] DNA鎖切断の効率的検出法 (2009)
  • Author(s): 佐藤勝也
  • Organizer: 第8回産学官連携推進会議
  • Place of Presentation: 京都
  • Year and Date: 2009-06-20
• [Presentation] プロテオーム解析による新規DNA修復関連タンパク質の同定 (2008)
  • Author(s): 大庭寛史
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-10
• [Presentation] プロテオーム解析による新規DNA修復関連タンパク質の同定 (2008)
  • Author(s): 大庭寛史
  • Organizer: 第31回日本分子生物学会年会第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2008-12-10
• [Presentation] 放射線抵抗性細菌デイノコッカス・ラジオデュランスの放射線応答機構に関与する新規タンパク質の発見 (2008)
  • Author(s): 大庭寛史
  • Organizer: 第3回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎
  • Year and Date: 2008-10-10
• [Presentation] 放射線抵抗性細菌デイノコッカス・ラジオデュランスの放射線応答機構に関与する新規タンパク質の発見 (2008)
  • Author(s): 大庭寛史
  • Organizer: 第3回高崎量子応用研究シンポジウム
  • Place of Presentation: 高崎市
  • Year and Date: 2008-10-10
• [Presentation] 放射線抵抗性細菌のDNA修復機構の研究 (2008)
  • Author(s): 佐藤勝也
  • Organizer: 第39回UV/EB研究会
  • Place of Presentation: 大阪
  • Year and Date: 2008-09-24
• [Presentation] Role of RecF in radiation-induced DNA double-strand breaks repair pathway in Deinococcus radiodurans (2008)
  • Author(s): Katsuya Satoh
  • Organizer: The 7th International conference on Extremophiles (Extremophiles 2008)
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2008-09-09
• [Presentation] Functional characterization of a novel modulator for radiation response mechanism in Deinococcus radiodurans (2008)
  • Author(s): Hirofumi Ohba
  • Organizer: The 7th International conference on Extremophiles (Extremophiles 2008)
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2008-09-09
• [Presentation] Three-dimensional architecture of the Deinococcus radiodurans PprA protein (2008)
  • Author(s): Issay Narumi
  • Organizer: The 7th International conference on Extremophiles (Extremophiles 2008)
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2008-09-09
• [Presentation] Role of RecF in radiation-induced DNA double-strand breaks repair pathway inDeinococcus radiodurans (2008)
  • Author(s): Katsuya Satoh
  • Organizer: The 7th International conference on Extremophiles (Extremophiles 2008)
  • Place of Presentation: Cape Town, South Africa
  • Year and Date: 2008-09-09
• [Remarks]
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• [Remarks]
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