Mechanisms of luteolysis via local GnRH as a follicular developing signal.

• Project/Area Number: 20780210
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Kitasato University
• Principal Investigator: YONEZAWA Tomohiro Kitasato University, 獣医学部・獣医学科, 講師 (10433715)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ゴナドトロピン放出ホルモン / アネキシンA5 / 黄体退行 / メタスチン / アポトーシス / 卵巣 / 性周期 / エストロジェン / アネキシン5

Research Abstract

Corpora lutea in rats start to regress in the afternoon of diestrus 2. In the present study, metastin and gonadotropin releasing hormone were expressed stage-specifically, which was caused by estrogen from next-developing follicles. These findings could make contributions to avoidance of breeding disorders and fertility treatments.

Research Products

• [Journal Article] The mechanism of GH pulse generation; involvements of sex steroids and/or NPY. (2010)
  • Author(s): Yonezawa T
  • Journal Title: Proc Japan Soc Animal Nutr Met 54 Pages: 13-24
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of annexin a5 in the mammary gland of rats: up-regulation of expression by pup removal. (2010)
  • Author(s): Rieanrakwong D, Yonezawa T, Kurusu S, Kawaminami M
  • Journal Title: J Vet Med Sci 72 Pages: 19-22
  • NAID: 130000134497
  • Peer Reviewed
• [Journal Article] Immunohistochemical localization of annexin a5 in the mammary gland of rats : up-regulation of expression by pup removal. (2010)
  • Author(s): Rieanrakwong D, et al.
  • Journal Title: J Vet Med Sci. 72 Pages: 19-22
  • Peer Reviewed
• [Journal Article] Group IVA phospholipase A(2) activity may mediate prostaglandin F(2alpha)-induced luteal regression in pseudopregnant rats. (2009)
  • Author(s): Kurusu S, Tokunaga N, Yonezawa T, Kawaminami M
  • Journal Title: Prostaglandins Other Lipid Mediat 90 Pages: 55-62
  • Peer Reviewed
• [Journal Article] Structural regression of the rat corpus luteum of pregnancy: relationship with functional regression, apoptotic cell death, and the suckling stimulus. (2009)
  • Author(s): Kurusu S, Suzuki K, Taniguchi K, Yonezawa T, Kawaminami M
  • Journal Title: Zoolog Sci 26 Pages: 729-734
  • NAID: 40016807055
  • Peer Reviewed
• [Journal Article] Differential regulation of phospholipase A(2) activity and prostaglandin E(2) synthesis in activated and non-activated corpora lutea of lactation by the presence and absence of suckling stimulus in rats. (2009)
  • Author(s): Kurusu S, Kamikubo M, Yonezawa T, Kawaminami M
  • Journal Title: Prostaglandins Other Lipid Mediat 90 Pages: 26-30
  • Peer Reviewed
• [Journal Article] Inhibition of ovulation by a lipoxygenase inhibitor involves reduced cyclooxygenase-2 expression and prostaglandin E2 production in gonadotropin-primed immature rats. (2009)
  • Author(s): Kurusu S, Jinno M, Ehara H, Yonezawa T, Kawaminami M
  • Journal Title: Reproduction 137 Pages: 59-66
  • Peer Reviewed
• [Journal Article] 第24回日本下垂体研究会の仕掛け (2009)
  • Author(s): 米澤智洋
  • Journal Title: 比較内分泌学 35 Pages: 333-334
  • NAID: 10025870830
• [Journal Article] Group IVA phospholipase A2 activity may mediate prostaglandin F2a-induced luteal regression in pseudopregnant rats (2009)
  • Author(s): Kurusu S, et al.
  • Journal Title: Prostaglandins other Lipid Mediat 90 Pages: 55-62
  • Peer Reviewed
• [Journal Article] Structural regression of rat corpus luteum of pregnancy : relationship with functional regression, apoptotic cell death, and suckling stimulus. (2009)
  • Author(s): Kurusu S, et al.
  • Journal Title: Zoological Sci 26 Pages: 729-734
  • Peer Reviewed
• [Journal Article] Differential regulation of phospholipase A2 activity and prostaglandin E2 synthesis in activated and nonactivated corpora lutea of lactation by presence and absence of suckling stimulus in rats (2009)
  • Author(s): Kurusu S, et al.
  • Journal Title: Prostaglandins other Lipid Mediat 90 Pages: 26-30
  • Peer Reviewed
• [Journal Article] Inhibition of ovulation by a lipoxygenase inhibitor involves reduced cyclooxygenase-2 expression and prostaglandin E2 production in gonadotropin-primed immature rats (2009)
  • Author(s): Kurusu S, et al.
  • Journal Title: Reproduction 137 Pages: 59-66
  • Peer Reviewed
• [Journal Article] Inhibition of ovulation by a lipoxygenase inhibitor involves reduced cycl ooxygenase-2 expression and prostaglandin E2 production in gonadotropi n-primed immature rats (2009)
  • Author(s): Kurusu S, et.al.
  • Journal Title: Reproduction 137 Pages: 59-66
  • Peer Reviewed
• [Presentation] Multiple protective effects of locally administered inhibitors of group IVA phospholipase A2 and cyclooxygenase-2 on pseudopregnant rat corpus luteum from prostaglandin F2α-induced regression. (2010)
  • Author(s): Kurusu S, Sonoda N, Nakahara M, Yonezawa T, Kawaminami M
  • Organizer: 14th International Congress of Endocrinology (ICE2010)
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-03-29
• [Presentation] Multiple protective effects of locally administered inhibitors of group IVA phospholipase A2 and cyclooxygenase-2 on pseudopregnant rat corpusluteum from prostaglandin F2α-induced regression (2010)
  • Author(s): Kurusu S, et al.
  • Organizer: 14th International Congress of Endocrinology (ICE2010)
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-03-29
• [Presentation] アネキシンA5ノックアウトマウスの生殖機能 (2009)
  • Author(s): 西村育宏, ドワンザイ・リエンラクオン, 米澤智洋, 久留主志朗, 武藤顕一郎, Ernst Poschl, 汾陽光盛
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取大学
  • Year and Date: 2009-09-25
• [Presentation] アネキシンA5ノックアウトマウスの生殖機能 (2009)
  • Author(s): 西村育宏, ら
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取大学農学部
  • Year and Date: 2009-09-25
• [Presentation] Metastin regulation of gonadotropin releasing hormone (GnRH) production in the mast cell. (2009)
  • Author(s): Rieanrakwong D, Yonezawa T, Kurusu S, Hasegawa Y, Kawaminami M
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 近畿大学
  • Year and Date: 2009-09-11
• [Presentation] Prostaglandin (PG) F2α誘導性の偽妊娠ラットの黄体退行における黄体内因性PGの役割:phospholipase A2及びcyclooxygenase阻害薬の局所投与の効果 (2009)
  • Author(s): 薗田憲史, 中原理人, 米澤智洋, 久留主志朗, 汾陽光盛
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 近畿大学
  • Year and Date: 2009-09-11
• [Presentation] ラット性周期黄体に発現するゴナドトロピン放出ホルモンはBaxとBcl-2の発現を制御して黄体のアポトーシスを誘導する (2009)
  • Author(s): 小形次人, 米澤智洋, 久留主志朗, 汾陽光盛
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 近畿大学
  • Year and Date: 2009-09-11
• [Presentation] Metastin regulation of gonadotropin releasing hormone (GnRH) production in the mast cell (2009)
  • Author(s): Rieanrakwong D, et al.
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 近畿大学農学部
  • Year and Date: 2009-09-11
• [Presentation] Prostaglandin(PG)F2α誘導性の偽妊娠ラットの黄体退行における黄体内因性PGの役割: phospholipase A2及びcyclooxygenase阻害薬の局所投与の効果 (2009)
  • Author(s): 薗田憲史, ら
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 近畿大学農学部
  • Year and Date: 2009-09-11
• [Presentation] ラット性周期黄体に発現するゴナドトロピン放出ホルモンはBaxとBcl-2の発現を制御して黄体のアポトーシスを誘導する (2009)
  • Author(s): 小形次人, ら
  • Organizer: 第102回日本繁殖生物学会
  • Place of Presentation: 近畿大学農学部
  • Year and Date: 2009-09-11
• [Presentation] ラット性周期黄体で産生される性腺刺激ホルモン放出ホルモンはプロラクチンの黄体刺激作用の感受性を低下させる (2009)
  • Author(s): 米澤智洋, 坂田恭平, 久留主志朗, 長谷川喜久, 汾陽光盛
  • Organizer: 第24回日本下垂体研究会
  • Place of Presentation: 青森県
  • Year and Date: 2009-08-27
• [Presentation] アネキシンA5ノックアウトマウスの表現型解析 (2009)
  • Author(s): 西村育宏, ドワンザイ・リエンラクオン, 米澤智洋, 久留主志朗, 武藤顕一郎, 汾陽光盛
  • Organizer: 第24回日本下垂体研究会
  • Place of Presentation: 青森県
  • Year and Date: 2009-08-27
• [Presentation] Hypogonadal (hpg)マウスを用いた性腺刺激ホルモン放出ホルモンシグナルのバイオマーカーとしてのアネキシンA5の可能性 (2009)
  • Author(s): 渡邊あい子, 米澤智洋, 久留主志朗, 長谷川喜久, 汾陽光盛
  • Organizer: 第24回日本下垂体研究会
  • Place of Presentation: 青森県
  • Year and Date: 2009-08-27
• [Presentation] ラット性周期黄体で産生される性腺刺激ホルモン放出ホルモンはプロラクチンの黄体刺激作用の感受性を低下させる (2009)
  • Author(s): 米澤智洋, ら
  • Organizer: 第24回日本下垂体研究会
  • Place of Presentation: 古牧温泉青森屋
  • Year and Date: 2009-08-27
• [Presentation] アネキシンA5ノックアウトマウスの表現型解析 (2009)
  • Author(s): 西村育宏, ら
  • Organizer: 第24回日本下垂体研究会
  • Place of Presentation: 古牧温泉青森屋
  • Year and Date: 2009-08-27
• [Presentation] Estrogen induced luteal gonadotropin releasing hormone (GnRH) causes refractoriness of newly formed corpus luteum to prolactin in the estrous cycle of rats. (2009)
  • Author(s): Yonezawa T, Kurusu S, Kawaminami M
  • Organizer: The Endocrine Society's 91st Annual Meeting
  • Place of Presentation: Washington, DC.
  • Year and Date: 2009-06-11
• [Presentation] Myxovirus (influenza virus) resistance 1 (Mx1) is involved in the growth suppression of LβT2 gonadotrope cells by activin. (2009)
  • Author(s): Kawaminami M, Hashimoto T, Terashima R, Yonezawa T, Hasegawa Y, Kurusu S
  • Organizer: The Endocrine Society's 91st Annual Meeting
  • Place of Presentation: Washington, DC.
  • Year and Date: 2009-06-11
• [Presentation] Mobilization of mast cells to the mammary gland is prerequisite for mammary involution after lactation: mediation of gonadotropin releasing hormone and antagonism by prolactin. (2009)
  • Author(s): Rieanrakwong D, Yonezawa T, Kurusu S, Hasegawa Y, Kawaminami M
  • Organizer: The Endocrine Society's 91st Annual Meeting
  • Place of Presentation: Washington, DC.
  • Year and Date: 2009-06-11
• [Presentation] Significance of mast cells in mammary involution after lactation : production of gonadotropin releasing hormone (GnRH) and its action in the mammary gland (2009)
  • Author(s): Kawaminami M, et al.
  • Organizer: The Endocrine Society's 91st Annual Meeting
  • Place of Presentation: Washington, DC
  • Year and Date: 2009-06-11
• [Presentation] Estrogen induced luteal gonadotropin releasing hormone (GnRH) causes refractoriness of newly formed corpus luteum to prolactin in the estrous cycle of rats (2009)
  • Author(s): Yonezawa T, et al.
  • Organizer: The Endocrine Society's 91st Annual Meeting
  • Place of Presentation: Washington, DC
  • Year and Date: 2009-06-11
• [Presentation] Mobilization of mast cells to the mammary gland is prerequisite for mammary involution after lactation : mediation of gonadotropin releasing hormone and antagonism by prolactin (2009)
  • Author(s): Rieanrakwong D, et al.
  • Organizer: The Endocrine Society's 91st Annual Meeting
  • Place of Presentation: Washington, DC
  • Year and Date: 2009-06-11
• [Presentation] Estrogenはラット性周期黄体のMetastinと性腺刺激ホルモン放出ホルモン(GnRH)の発現を促進して黄体退行を引き起こす (2008)
  • Author(s): 米澤智洋, 久留主志朗, 汾陽光盛
  • Organizer: 第101回日本繁殖生物学会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-09-18
• [Presentation] Involvement of local gonadotropin releasing hormone (GnRH) in involution of lactating mammary epithelial cells of rats. (2008)
  • Author(s): Rieanrakwong D, Yonezawa T, Kurusu S, Hasegawa Y, Kawaminami M
  • Organizer: 第101回日本繁殖生物学会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-09-18
• [Presentation] Involvement of local gonadotropin releasing hormone(GnRH)in involuti on of lactating mammary epithelial cells of rats (2008)
  • Author(s): Rieanrakwong D, et.al.
  • Organizer: 第101回日本繁殖生物学会
  • Place of Presentation: 九州大学
  • Year and Date: 2008-09-18
• [Presentation] 性腺刺激ホルモン放出ホルモン(GnRH)による増殖調節 (2008)
  • Author(s): 汾陽光盛, 小野山一郎, 米澤智洋, 久留主志朗
  • Organizer: 第23回日本下垂体研究会
  • Place of Presentation: 東京
  • Year and Date: 2008-08-30
• [Presentation] Inhibition of ovulation by a lipoxygenase inhibitor is associated with reduced cyclooxygenase-2 expression and prostaglandin E2 production in gonadotropin-primed immature rat model. (2008)
  • Author(s): Kurusu S, Jinno M, Ehara H, Yonezawa T, Kawaminami M
  • Organizer: Gordon research conference
  • Place of Presentation: New Hampshire
  • Year and Date: 2008-08-07
• [Presentation] Inhibition of ovulation by a lipoxygenase inhibitor is associated with reduced cyclooxygenase-2 expression and prostaglandin E2 production in gonadotronin-nrimed immature rat model (2008)
  • Author(s): Kurusu S, et.al.
  • Organizer: Gordon research conference
  • Place of Presentation: New Hampshire, USA
  • Year and Date: 2008-08-07
• [Presentation] Involvement of corpus luteum metastin in gonadotropin releasing hormone (GnRH) induced luteolysis in cycling rats. (2008)
  • Author(s): Yonezawa T, Kurusu S, Kawaminami M
  • Organizer: The Endocrine Society's 90th Annual Meeting
  • Place of Presentation: California
  • Year and Date: 2008-06-16
• [Presentation] Gonadotropin releasing hormone (GnRH) receptor stimulation of Mx1 gene expression causes suppression of LβT2 gonadotrope cell growth. (2008)
  • Author(s): Kawaminami M, Onoyama I, Yonezawa T, Kurusu S
  • Organizer: The Endocrine Society's 90th Annual Meeting
  • Place of Presentation: California
  • Year and Date: 2008-06-16
• [Presentation] Involvement of corpus luteum metastin in gonadotropin releasing hormcne(GnRH)induced luteolysis in cycling rats (2008)
  • Author(s): Yonezawa T, et.al.
  • Organizer: The Endocrine Society's 90th Annual Meeting
  • Place of Presentation: California, USA
  • Year and Date: 2008-06-16
• [Presentation] Gonadotropin releasing hormone(GnRH)receptor stimulation of Mxl gene expression causes suppression of LRT2 gonadotrope cell growth (2008)
  • Author(s): Kawaminami M, et.al.
  • Organizer: The Endocrine Society's 90th Annual Meeting
  • Place of Presentation: California, USA
  • Year and Date: 2008-06-16
• [Book] アポトーシス実験法(獣医生化学実験改 訂第3版) (2009)
  • Author(s): 米澤智洋(分担執筆)
  • Publisher: チクサン出版社
• [Book] 繁殖内分泌に関する実習, 実験で学ぶ生理学(懇談会編) (2009)
  • Author(s): 米澤智洋(分担執筆)
  • Publisher: 学窓社
• [Book] アポトーシス実験法(獣医生化学実験改訂第3版) (2009)
  • Author(s): 米澤智洋
  • Publisher: チクサン出版社
• [Book] アポトーシス実験法(獣医生化学実験) (2009)
  • Author(s): 米澤智洋, 他
  • Publisher: チクサン出版社
• [Remarks]
  • URL: http://www2.vmas.kitasato-u.ac.jp/physiology/
• [Patent(Industrial Property Rights)] 骨成長に対するエストロゲン作用の判定法 (2009)
  • Inventor(s): 米澤智洋、武藤顕一郎
  • Industrial Property Rights Holder: 北里大学
  • Industrial Property Number: 2009-109231
  • Filing Date: 2009-04-01
• [Patent(Industrial Property Rights)] 骨成長に対するエストロゲン作用の判定法 (2009)
  • Inventor(s): 米澤智洋, ら
  • Industrial Property Rights Holder: 北里大学
  • Industrial Property Number: 2009-109231
  • Filing Date: 2009-04-01