Functional analysis of synaptotagmin IIas a receptor for Clostridium botulinum type B neurotoxin expressed neuroendocrine-like cells
| Project/Area Number |
20780220
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied veterinary science
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
KOHDA Tomoko Osaka Prefecture University, 生命環境科学研究科, 助教 (80336809)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ボツリヌス神経毒素 / 受容体 / シナプトタグミン / PC12細胞 / ボツリヌス毒素 / ガングリオシド / リサイクリング / シナプトブレビン |
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| Research Abstract |
We generated StgII expressed PC12 cells transiently and applied to understand more precisely how BoNT/B recognizes the receptor and enters neurons. The functional entry of BoNT/B was determined by monitoring VAMP2 amount in the cells. We examined the role of ganglioside, StgII-expressing cells were treated with ganglioside exogenously. The sensitivity of the cells was higher than of non-treated cells. These results support the notion that StgII and ganglioside cooperate to play a role in BoNT/B receptor.
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Report
(3 results)
Research Products
(5 results)
