The mechanism of viral induced apoptosis in mammalian airway epithelial cells infected with highly pathogenic avian influenza virus H5N1

• Project/Area Number: 20790710
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Infectious disease medicine
• Research Institution: Osaka University
• Principal Investigator: DAIDOJI Tomo Osaka University, 微生物病研究所, 特任研究員 (80432433)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 高病原性鳥インフルエンザ / アポトーシス / 高病原性トリインフルエンザ

Research Abstract

To elucidate the mechanism of H5N1 pathogenesis, we prepared primary airway epithelial cells from alveolar tissues from 1-year-old pigs and generated recombinant H5N3 viruses possessing H5N1 hemagglutinin (HA) by using reverse genetics system. After infection with the recombinant H5N3 viruses, H5N1 HA protein played a critical role in inducing caspase-dependent apoptosis in infected porcine alveolar epithelial cells. In addition, H5N1 HA1 region (84-340 amino acids) was essential for induction of caspase-dependent apoptosis in infected cells.

Research Products

• [Journal Article] Highly pathogenic H5N1 avian influenza virus induces extracellular Ca2+ influx, leading to apoptosis in avian cells (2010)
  • Author(s): Ueda M, Daidoji T, Du A, Yang CS, Ibrahim MS, Ikuta K, Nakaya T.
  • Journal Title: J Virol 6 Pages: 3068-3078
  • Peer Reviewed
• [Journal Article] Highly Pathogenic H5N1 Avian Influenza Virus Induces Extracellular Ca2+ Influx, Leading to Apoptosis in Avian Cells (2010)
  • Author(s): Ueda M, Daidoji T, Du A, Yang CS, Ibrahim MS, Ikuta K, Nakaya T.
  • Journal Title: J Virol. 6(Now in press)
  • Peer Reviewed
• [Journal Article] Broad and potent anti-influenza virus spectrum of gallocatechin-3-O-gallate-monopalmitate (2009)
  • Author(s): Kaihatsu K, Mori S, Matsumura H, Daidoji T, Kawakami C, Kurata H, Nakaya T, Kato N.
  • Journal Title: J Mol Genet Med. 3 Pages: 195-197
  • Peer Reviewed
• [Journal Article] Disposition of flavonoids via enteric recycling: UDP-glucuronosyltransferase (UGT) 1As deficiency in Gunn rats is compensated by increases in UGT2Bs activities (2009)
  • Author(s): Wang SW, Kulkarni KH, Tang L, Wang JR, Yin T, Daidoji T, Yokota H, Hu M.
  • Journal Title: J Pharmacol Exp Ther 329 Pages: 1023-1031
  • Peer Reviewed
• [Journal Article] Detection of circulating Asian H5N1 viruses by a newly established monoclonal antibody (2009)
  • Author(s): Du A, Daidoji T, Koma T, Ibrahim MS, Nakamura S, de Silva UC, Ueda M, Yang CS, Yasunaga T, Ikuta K, Nakaya T.
  • Journal Title: Biochem Biophys Res Commun 378 Pages: 197-202
  • Peer Reviewed
• [Journal Article] Disposition of flavonoids via enteric recycling : UDP-glucuronosyltrans ferase (UGT) 1As deficiency in Gunn rats is compensated by increases in UGT2Bs activities. (2009)
  • Author(s): Wang SW, Kulkarni KH, Tang L, Wang JR, Yin T, Daidoji T, Yokota H, Hu M.
  • Journal Title: J Pharmacol Exp Ther. 329 Pages: 1023-1031
  • Peer Reviewed
• [Journal Article] Broad and potent anti-influenza virus spectrum of epigallocatechin-3-O-gallate-monopalmitate. (2009)
  • Author(s): Kaihatsu K, Mori S, Matsumura H, Daidoji T, Kawakami C, Kurata H, Nakaya T, Kato N.
  • Journal Title: J Mol Genet Med. 2 Pages: 195-197
  • Peer Reviewed
• [Journal Article] Detection of circulating Asian H5N1 viruses by a newly established monoclonal antibody (2009)
  • Author(s): Du A, Daidoji T, Koma T, Ibrahim MS, Nakamura S, de Silva UC, Ueda M, Yang CS, Yasunaga T, Ikuta K, Nakaya T.
  • Journal Title: Biochemical and Biophysical Research Communications 378 Pages: 197-202
  • Peer Reviewed
• [Journal Article] H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells (2008)
  • Author(s): Daidoji T, Koma T, Du A, Yang CS, Ueda M, Ikuta K, Nakaya T.
  • Journal Title: J Virol 82 Pages: 11294-11307
  • Peer Reviewed
• [Journal Article] Differential metabolism of 4-n- and 4-tert-octylphenols in perfused rat liver (2008)
  • Author(s): Nomura S, Daidoji T, Inoue H, Yokota H
  • Journal Title: Life Sci. 83 Pages: 223-228
  • Peer Reviewed
• [Journal Article] Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus (2008)
  • Author(s): Ueda M, Yamate M, Du A, Daidoji T, Okuno Y, Ikuta K, Nakaya T.
  • Journal Title: Virus Res. 136 Pages: 91-97
  • Peer Reviewed
• [Journal Article] H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells. J Virol.,82, 11294-11307 (2008)
  • Author(s): Daidoji T, Koma T, Du A, Yang CS, Ueda M, Ikuta K, Nakaya T.
  • Journal Title: Journal of Virology 82 Pages: 11294-11307
  • Peer Reviewed
• [Presentation] 高病原性鳥インフルエンザウイルスH5N1の細胞傷害誘導に関係するHA領域の検討 (2009)
  • Author(s): 大道寺智、中村昇太、Chandimal de Silva、上田真世、ドゥアナリワ、楊成松、生田和良、中屋隆明
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル(東京)
  • Year and Date: 2009-10-25
• [Presentation] One-step immunochromatographic assay for asian H5N1 detection (2009)
  • Author(s): Tomo Daidoji, Anariwa Du, Yusuke Shibai, Daisuke Ito, Mayo Ueda, Cheng-Song Yang, Hisahiko Iwamoto, Kazuyoshi Ikuta, Takaaki Nakaya
  • Organizer: International Joint Forum on Infectious Diseases
  • Place of Presentation: Siam City Hotel, Bangkok, Thailand
  • Year and Date: 2009-09-16
• [Presentation] 高病原性トリインフルエンザウイルスH5N1は哺乳動物呼吸器上皮細胞に対してアポトーシスを誘導する (2008)
  • Author(s): 大道寺智、駒貴明、楊成松、デュアナリワ、上田真世、生田和良、中屋隆明
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-11
• [Presentation] 高病原性トリインフルエンザウイルスH5N1の細胞傷害メカニズムの検討 (2008)
  • Author(s): 大道寺智、駒貴明、楊成松、Du Anariwa、上田真世、生田和良、中屋隆明
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山コンベンションセンター
  • Year and Date: 2008-10-28
• [Presentation] H5N1 avian influenza virus induces apoptotic cell death in mammalian airway epithelial cells (2008)
  • Author(s): Tomo Daidoji, Takaaki Koma, Anariwa Du, Cheng-Song Yang, Mayo Ueda, Kazuyoshi Ikuta, Takaaki Nakaya
  • Organizer: FORUM OF THE NETWORK OF RESEARCH CENTERS ON INFECTIOUS DISEASES
  • Place of Presentation: National Institute of Hygiene and Epidemiology (NIHE), Hanoi-Vietnam
  • Year and Date: 2008-10-06
• [Presentation] 茶カテキンから抗インフルエンザ分子の創製を目指して (2008)
  • Author(s): 開發邦宏、森修一、加藤修雄、大道寺智、中屋隆明
  • Organizer: 第5回日本カテキン学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2008-08-22