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L-serine regulation of RANK signaling machinery and its role in bone metabolism

Research Project

Project/Area Number 20791038
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Orthopaedic surgery
Research InstitutionNara Institute of Science and Technology

Principal Investigator

OGAWA Takuya  Nara Institute of Science and Technology, バイオサイエンス研究科, 助教 (30457147)

Project Period (FY) 2008 – 2009
Project Status Completed (Fiscal Year 2009)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsアミノ酸 / セリン / 細胞・組織 / 発生・分化 / 発現制御 / シグナル伝達 / 骨・軟骨代謝学 / 破骨細胞 / L-セリン / 分化 / RANK
Research Abstract

Osteoclasts play central roles in the development and remodeling of bone. In addition to the stimulation by RANKL, serine in the culture medium is necessary for the in vitro differentiation of osteoclasts from their precursors. We conducted a series of experiments to understand the regulatory mechanism of osteoclast differentiation by serine as well as its physiological significance, and found the association between serine transport and cellular uptake of other amino acids and serine deprivation suppresses the differentiation through the reduction of other several intracellular amino acids and the downregulation of RANKL receptor, RANK.

Report

(3 results)
  • 2009 Annual Research Report   Final Research Report ( PDF )
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2009 2008 Other

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (18 results)

  • [Journal Article] Identification of a novel L-serine analog that suppresses osteoclastogenesis in vitro and bone turnover in vivo.2009

    • Author(s)
      Bahtiar A, 他5名, Ogawa T(7番目), Takeya T.
    • Journal Title

      J Biol Chem. 284

      Pages: 34157-34166

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] 細胞外L-セリンは破骨細胞分化におけるRANKL-RANKシグナル伝達系活性化に必須である2009

    • Author(s)
      小川拓哉(1番目), 他7名
    • Journal Title

      アミノ酸研究 3

      Pages: 63-65

    • Related Report
      2009 Final Research Report
  • [Journal Article] Identification of a Novel L-Serine Analog That Suppresses Osteoclastogenesis in Vitro and Bone Turnover in Vivo.2009

    • Author(s)
      Anton Bahtiar
    • Journal Title

      J.Biol.Chem 284

      Pages: 34157-34166

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 細胞外L-セリンは破骨細胞分化におけるRANKL-RANKシグナル伝達系活性化に必須である2009

    • Author(s)
      小川拓哉
    • Journal Title

      アミノ酸研究 3

      Pages: 63-65

    • Related Report
      2009 Annual Research Report
  • [Journal Article] Potential involvement of Twist2 and Erk in the regulation of osteoblastogenesis by HB-EGF-EGFR signaling.

    • Author(s)
      Nakamura T, 他4名, Ogawa T(6番目), Takeya T, Ishida-Kitagawa N.
    • Journal Title

      Cell Struct Funct. (in press)

    • NAID

      130004053875

    • Related Report
      2009 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential Involvement of Twist2 and Erk in the Regulation of Osteoblas togenesis by HB-EGF-EGFR Signaling

    • Author(s)
      Takashi Nakamura
    • Journal Title

      Cell Struct.Funct. (In press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Identification of a novel inhibitor for osteoclastogenesis in vitro and bone turnover in vivo2009

    • Author(s)
      Bahtiar A(代:小川拓哉)
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-11
    • Related Report
      2009 Final Research Report
  • [Presentation] マウス前駆破骨細胞の細胞内アミノ酸レベルに対する細胞外L-セリンの作用2009

    • Author(s)
      西田織衣
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Final Research Report
  • [Presentation] 前駆破骨細胞のRANK発現制御におけるL-セリンの役割2009

    • Author(s)
      的場祐衣
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2009 Final Research Report
  • [Presentation] 細胞外L-セリンは破骨細胞分化におけるRANKL-RANKシグナル伝達系活性化に必須である2009

    • Author(s)
      小川拓哉
    • Organizer
      日本アミノ酸学会第3回学術大会
    • Place of Presentation
      京都府立大学
    • Year and Date
      2009-09-30
    • Related Report
      2009 Final Research Report
  • [Presentation] Lactoperoxidase, a Major Component of Milk Basic Protein, attenuates in vitro Osteoclast Differentitaion.2009

    • Author(s)
      Ogawa T
    • Organizer
      31st Annual Meeting of The American Society for Bone and Mineral Research
    • Place of Presentation
      Colorado Convention Center
    • Year and Date
      2009-09-13
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification of a Novel L-Serine Analog that Suppresses Osteoclastogenesis in vitro and Bone Turnover in vivo.2009

    • Author(s)
      Bahtiar A
    • Organizer
      第27回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2009-07-25
    • Related Report
      2009 Final Research Report
  • [Presentation] Identification of a Novel L-Serine Analog That Suppresses Osteoclastogenesis in Vitro and Bone Turnover in Vivo.2009

    • Author(s)
      Anton Bahtiar
    • Organizer
      第27回日本骨代謝学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2009 Annual Research Report
  • [Presentation] Lactoperoxidase, a Major Component of Milk Basic Protein, Attenuates in Vitro Osteoclast Differentiation2009

    • Author(s)
      小川拓哉
    • Organizer
      the 31th Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Denver, CO, U.S.A.
    • Related Report
      2009 Annual Research Report
  • [Presentation] 細胞外L-セリンは破骨細胞分化におけるRANL-RANKシグナル伝達系活性化に必須である2009

    • Author(s)
      小川拓哉
    • Organizer
      第3回日本アミノ酸学会学術大会
    • Place of Presentation
      京都
    • Related Report
      2009 Annual Research Report
  • [Presentation] L-Serine Regulation of RANK Gene Expression in Osteclast Precursor Cells.2009

    • Author(s)
      的場祐衣
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Effects of Extracellular L-Serine on Intracellular Amino Acid Levels in Mouse Osteoclast Precursors.2009

    • Author(s)
      西田織衣
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] Identification of a Novel Inhibitor for Osteoclastogenesis in Vitro and Bone Turnover in Vivo.2009

    • Author(s)
      Anton Bahtiar(代:小川拓哉)
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2009 Annual Research Report
  • [Presentation] L-Serine modulates RANKL/RANK signaling cascade and plays an essential role in osteoclastogenesis in vitro2008

    • Author(s)
      Bahtiar A
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2008-12-10
    • Related Report
      2009 Final Research Report
  • [Presentation] L-セリンによるRANKL-RANKシグナル伝達系活性化機構の解析2008

    • Author(s)
      Ogawa T
    • Organizer
      第26回日本骨代謝学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2008-10-29
    • Related Report
      2009 Final Research Report
  • [Presentation] A novel role of L-serine for the activation of receptor activator of nuclear factor kappa B ligand (RANKL)-RANK signaling machinery in osteoclastogenesis in Vitro2008

    • Author(s)
      Ogawa T
    • Organizer
      30th Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Palais des congres de Montreal
    • Year and Date
      2008-09-13
    • Related Report
      2009 Final Research Report
  • [Presentation] A Novel Role of L-Serine for the Activation of Receptor Activator of Nuclear Factor Kappa B Ligand (RANKL)-RANK Signaling Machinery in Osteoclastogenesis in yitro2008

    • Author(s)
      小川拓哉
    • Organizer
      30^<th> Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Montr・ al, Qu・ bec, Canada
    • Related Report
      2008 Annual Research Report
  • [Presentation] L-セリンによるRANKL-RANKシグナル伝達系活性化機構の解析2008

    • Author(s)
      小川 拓哉
    • Organizer
      第26回日本骨代謝学会
    • Place of Presentation
      大阪
    • Related Report
      2008 Annual Research Report
  • [Presentation] L-Serine modulates RANKL/RANK signaling cascade and plays an essential role in osteoclastogenesis in vitro2008

    • Author(s)
      Anton Bahtiar
    • Organizer
      第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
    • Place of Presentation
      神戸
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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