Autologous rabbit bone marrow-derived cells implanted into radiation-injured urinary bladders recovery functional tissue structures

• Project/Area Number: 20791104
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Urology
• Research Institution: Shinshu University
• Principal Investigator: IMAMURA Tetsuya Shinshu University, 医学部, 研究員 (00467143)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 再生医療 / 膀胱組織再生 / 放射線照射傷害 / 自己骨髄由来細胞移植 / フラッシュアウト法 / 細胞培養 / 自己細胞移植 / 骨髄細胞 / 放射線傷害 / 膀胱 / 膀胱内圧 / ウサギ

Research Abstract

We investigated to determine if implantation of autologous bone marrow-derived cells into radiation-injured urinary bladders recovered functional tissue structures. In this study, we attempted to produce the radiation-injured urinary bladders, however the proper models were not established. We have established the methods to obtain high quality autologous bone marrow-derived cells. Therefore, our projects had been developed.

Research Products

• [Journal Article] CXCR3 Binding Chemokine and TNFSF14 Over Expression in Bladder Urothelium of Patients With Ulcerative Interstitial Cystitis. (2010)
  • Author(s): Teruyuki Ogwa, Toshiki Homma, (他4名, 6番目)
  • Journal Title: The Journal of Urology 183(掲載決定) Pages: 1206-1212
  • Peer Reviewed
• [Journal Article] Bone Marrow-Derived Cells Implanted into Freeze-Injured Urinary Bladders Rec-onstruct Functional Smooth Muscle Layers (2010)
  • Author(s): 今村哲也、石塚修、山本徳則、後藤百万、西澤理
  • Journal Title: LUTS 2 Pages: 1-10
  • Peer Reviewed
• [Journal Article] Bone Marrow-Derived Cells Implanted into Freeze-njured Urinary Bladders Reconstructs Functional Smooth Muscle Layers. (2009)
  • Author(s): Tetsuya Imamura, Osamu Ishizuka, (他3名)
  • Journal Title: LUTS (掲載決定)
  • Peer Reviewed
• [Journal Article] Rat Bladders Augmented with a Novel Bovine Pericardium-Derived Biomaterial Reconstruct Functional Tissue Structures. (2009)
  • Author(s): Yuji Mimura, Tetsuya Imamura, (他4名)
  • Journal Title: LUTS (掲載決定)
  • Peer Reviewed
• [Journal Article] Differentiation of Smooth Muscle Cells from Human Amniotic Mesenchymal Cells Implanted in The Freeze-injured Mouse Urinary Bladder. (2009)
  • Author(s): Tomonori Minagawa, Tetsuya Imamura, (他4名)
  • Journal Title: European Urology (掲載決定)
  • NAID: 120007107384
  • Peer Reviewed
• [Journal Article] Autologous Bone-Marrow-Derived Mesenchymal Stem Cell Transplantation into Injured Rat Urethral Sphincter. (2009)
  • Author(s): Yoshiaki Kinebuchi, Naoki Aizawa, (他4名, 3番目)
  • Journal Title: International Journal of Urology (掲載決定)
  • Peer Reviewed
• [Journal Article] The Microenvironment of Freeze-injured Mouse Urinary Bladders Enables Successful Tissue Engineering. (2009)
  • Author(s): Tetsuya Imamura, Tomonori Yamamoto, (他3名)
  • Journal Title: Tissue Engineering 15(11) Pages: 3367-3375
  • NAID: 120007110471
  • Peer Reviewed
• [Journal Article] An Extract (THC-002) of Ba-Wei-Die-Huang-Wan Inhibits Expression of Tachykinins, and P2X3 and TRPV1 Receptors, and Inhibits ATP-Induced Detrusor Overactivity in Spontaneously Hypertensive Rats. (2009)
  • Author(s): Tetsuya Imamura, Osamu Ishizuka, (他7名)
  • Journal Title: Neurourology and Urodynamics 28 Pages: 529-534
  • Peer Reviewed
• [Journal Article] Stimulation of Skin Menthol Receptors Stimulates Detrusor Activity in Conscious Rats. (2009)
  • Author(s): Chen Zhong, Osamu Ishizuka, (他6名, 3番目)
  • Journal Title: Neurourology and Urodynamics (掲載決定)
  • Peer Reviewed
• [Journal Article] Role of α1-Adrenergic Receptors in Detrusor Overactivity Induced by Cold Stress in Conscious Rat. (2009)
  • Author(s): Chen Zhong, Osamu Ishizuka, (他5名, 3番目)
  • Journal Title: Neurourology and Urodynamics 28 Pages: 251-256
  • Peer Reviewed
• [Journal Article] The Microenvironment of Freeze-Injured Mouse Urinary Bladders Enables Succes-sful Tissue Engineering (2009)
  • Author(s): 今村哲也、山本徳則、石塚修、後藤百万、西澤理
  • Journal Title: Tissue Engineering : Part A 15 Pages: 3367-3375
  • Peer Reviewed
• [Journal Article] Implanted Mouse Bone Marrow-Derived Cells Reconstruct Layered Smooth Muscle Structures in Injured Urinary Bladders. (2008)
  • Author(s): Tetsuya Imamura, Yoshiaki Kinebuchi, (他4名)
  • Journal Title: Cell Transplantation 17 Pages: 267-278
  • Peer Reviewed
• [Journal Article] Cold Environment Stress Induces Detrusor Overactivity via Resiniferatoxin-Sensitive Nerves in Conscious Rats. (2008)
  • Author(s): Tetsuya Imamura, Osamu Ishizuka, (他6名)
  • Journal Title: Neurourology and Urodynamics 27 Pages: 348-352
  • Peer Reviewed
• [Journal Article] Gosha-jinki-gan Reduces Transmitter Protein and Sensory Receptors Associated with C Fiber Activation Induced by Acetic Acid in Rat Urinary Bladder. (2008)
  • Author(s): Tetsuya Imamura, Osamu Ishizuka, (他6名)
  • Journal Title: Neurourology and Urodynamics 27 Pages: 832-837
  • NAID: 120007110362
  • Peer Reviewed
• [Presentation] ウサギ腹圧性尿失禁モデルを用いた自家骨髄細胞移植による尿失禁改善への検討 (2010)
  • Author(s): 今村哲也
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
  • Year and Date: 2010-03-19
• [Presentation] ウサギ腹圧性尿失禁モデルを用いた自己骨髄由来細胞移植による尿失禁改善への検討 (2010)
  • Author(s): 今村哲也
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2010-03-19
• [Presentation] Mice Freeze-injured Urinary Bladders Provide a Microenvironment for Bone Marrow-Derived Cells to Regenerate Smooth Muscle Layers (2009)
  • Author(s): 今村哲也
  • Organizer: The 4th Pan-Pacific Continence Society Meeting
  • Place of Presentation: 福岡
  • Year and Date: 2009-09-10
• [Presentation] 骨髄細胞移植による膀胱平滑筋層再生が生じた凍結傷害膀胱組織の検討 (2009)
  • Author(s): 今村哲也
  • Organizer: 第97回日本泌尿器科学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2009-04-17
• [Presentation] 凍結傷害を受けた膀胱組織での骨髄細胞移植による膀胱平滑筋層の再構築における環境の解明 (2009)
  • Author(s): 今村哲也
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-06
• [Presentation] 凍結傷害を受けた膀胱組織での骨髄細胞移植による膀胱平滑筋層の再構築における環境の解明 (2009)
  • Author(s): 今村哲也
  • Organizer: 第8回日本再生医療学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2009-03-06