Study for the differential regulation of pituitary gonadotropins

• Project/Area Number: 20791144
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Obstetrics and gynecology
• Research Institution: Shimane University
• Principal Investigator: KANASAKI Haruhiko Shimane University, 医学部, 講師 (10325053)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: ゴナドトロピン / 下垂体 / GnRH / 生殖内分泌 / PACAP / PAC1 / ERK / MKP1

Research Abstract

Gene expression for PACAP and its own PAC1 receptor (PAC1R) were examined by pulsatile GnRH stimulation using perifusion system. In static culture, GnRH stimulated the expression of PACAP and PAC1R in gonadotroph cell line, LbT2. In pulsatile GnRH stimulation, low frequency of GnRH pulse preferentially increased PACAP and PAC1R expressions compared to that by low frequency GnRH pulse. PACAP increased both LH and FSH subunits dose-dependent manners. PACAP and PAC1R might be involved in GnRH pulse frequency-dependent gonadotropn subunit expressions.

Research Products

• [Journal Article] Possible Involvement of PACAP and PACAP type 1 Receptor in GnRH-Induced FSH -Subunit Gene Expression. (2011)
  • Author(s): Kanasaki H, Purwana IN, Oride A, Mijiddorj T Miyazaki K.
  • Journal Title: Regulatory Peptides 167 Pages: 227-232
• [Journal Article] Induction of Dual Specificity Phosphatase 1 (DUSP1) by Pulsatile Gonadotropin-Releasing Hormone Stimulation and the Role of Gonadotropin Subunit Expression. (2011)
  • Author(s): Purwana IN, Kanasaki H, Oride A, Mijiddorj T Miyazaki K.
  • Journal Title: Biology of Reproduction in press
• [Journal Article] Effects of metformin administration on plasma gonadotropin levels in women with infertility, with an in vitro study of the direct effects on the pituitary gonadotrophs. (2010)
  • Author(s): Oride A, Kanasaki H, Purwana IN, Miyazaki K.
  • Journal Title: Pituitary 13 Pages: 236-241
• [Journal Article] Induction of Dual Specificity Phosphatase 1 (DUSP1) by Gonadotropin-Releasing Hormone (GnRH) and the Role for Gonadotropin Subunit Gene Expression in Mouse Pituitary Gonadotroph LbetaT2 Cells. (2010)
  • Author(s): Purwana IN, Kanasaki H, Oride A, Miyazaki K.
  • Journal Title: Biology of Reproduction 82 Pages: 352-362
• [Journal Article] GnRH-induced PACAP and PAC1 Receptor Expression in Pituitary Gonadotrophs : A Possible Role in the Regulation of Gonadotropin Subunit Gene Expression. (2010)
  • Author(s): Purwana IN, Kanasaki H, Oride A, Mijiddorj T, Shintani N, Hashimoto T, Baba A, Miyazaki K.
  • Journal Title: Peptides 31 Pages: 1748-1755
• [Journal Article] Induction of Dual Specificity Phosphatase 1 (DUSP1) by Gonadotropin-Releasing Hormone (GnRH) and the Role for Gonadotropin Subunit Gene Expression in Mouse Pituitary Gonadotroph LbetaT2 Cells. (2010)
  • Author(s): Purwana IN., Kanasaki H., et al.
  • Journal Title: Biology of Reproduction Volume: 82 Pages: 352-362
  • Peer Reviewed
• [Journal Article] GnRH-induced PACAP and PAC1 Receptor Expression in Pituitary Gonadotrophs : A Possible Role in the Regulation of Gonadotropin Subunit Gene Expression. (2010)
  • Author(s): Purwana IN., Kanasaki H., et al
  • Journal Title: Peptides Volume: 31 Pages: 1748-1755
  • Peer Reviewed
• [Journal Article] Effects of metformin administration on plasma gonadotropin levels in women with infertility, with an in vitro study of the direct effects on the pituitary gonadotrophs. (2010)
  • Author(s): Oride A, Kanasaki H., et.al
  • Journal Title: Pituitary Volume: 13 Pages: 236-241
  • Peer Reviewed
• [Journal Article] Follistatin, induced by Thyrotropin-releasing hormone (TRH), plays no role in prolactin expression but affects gonadotropin FSHss expression as a paracrine factor in pituitary somatolactotroph GH3 cells. (2009)
  • Author(s): Oride A, Kanasaki H, Purwana IN, Miyazaki K.
  • Journal Title: Regulatory Peptides 156 Pages: 65-71
• [Journal Article] Pulse frequency-dependent gonadotropin gene expression by adenylate cyclase-activation polypeptide 1 in perifused mouse pituitary LβT2 cells. (2009)
  • Author(s): Kanasaki H, Mutiara S, Oride A, Purwana IN, Miyazaki K.
  • Journal Title: Biology of Reproduction 81 Pages: 465-472
• [Journal Article] Possible Involvement of Mitogen-Activated Protein Kinase Phosphatase-1 (MKP-1) in Thyrotropin-Releasing Hormone (TRH)-induced Prolactin Gene Expression. (2009)
  • Author(s): Oride A, Kanasaki H, Purwana IN, Miyazaki K.
  • Journal Title: Biochemical and Biophysical Research Communications 382 Pages: 663-667
• [Journal Article] Follistatin gene expression by gonadotropin-releasing hormone : role for cyclic AMP and mitogen-activated protein kinase signaling pathway in clonal gonadotroph LβT2 cells. (2009)
  • Author(s): Mutiara S, Kanasaki H, Oride A Shimasaki S, Yamamoto H, Miyazaki K
  • Journal Title: Molecular and Cellular Endocrinology 307 Pages: 125-32
• [Journal Article] Pulse frequency-dependent gonadotropin gene expression by adenylate cyclase-activation polypeptide 1 in perifused mouse pituitary LβT2 cells. (2009)
  • Author(s): Kanasaki H, Mutiara S., et al.
  • Journal Title: Biology of Reproduction 81 Pages: 465-472
  • Peer Reviewed
• [Journal Article] Follistatin gene expression by gonadotropin-releasing hormone : role for cyclic AMP and mitogen-activated protein kinase signaling pathway in clonal gonadotroph LβT2 cells. (2009)
  • Author(s): Mutiara S, Kanasaki H., et. al
  • Journal Title: Molecular and Cellular Endocrinology 307 Pages: 125-132
  • Peer Reviewed
• [Journal Article] Possible Involvement of Mitogen-Activated Protein Kinase Phosphatase-1(MKP-1)in Thyrotropin-Releasing Hormone(TRH)-induced Prolactin Gene Expression. (2009)
  • Author(s): Oride A, Kanasaki H., et. Al
  • Journal Title: Biochemical and Biophysical Research Communications 382 Pages: 663-667
  • Peer Reviewed
• [Journal Article] Activation of Extracellular Signal-Regulated Kinase (ERK) and Induction of Mitogen-Activated Protein Kinase Phosphatase 1 (MKP1) by Perifused Thyrotropin-Releasing Hormone (TRH) Stimulation in Rat Pituitary GH3 Cells. (2008)
  • Author(s): Oride A, Kanasaki H, Mutiara S, Purwana IN, Miyazaki K
  • Journal Title: Molecular and Cellular Endocrinology 296 Pages: 78-86
• [Journal Article] Up-regulation of gonadotropin α-subunit gene by phosphatidylinositol 3-kinase inhibitors in clonal gonadotroph cells. (2008)
  • Author(s): Kanasaki H, Mutiara S, Oride A, Miyazaki K
  • Journal Title: Neuroendocrinology Letters 29 Pages: 529-535
• [Journal Article] The Involvement of Phosphatidylinositol 3-Kinase in Gonadotropin- Releasing Hormone Induced Gonadotropin α- and FSHβ Subunit Gene Expression in Clonal Gonadotroph LβT2 Cells. (2008)
  • Author(s): Mutiara S, Kanasaki H, Harada T, Oride A, Miyazaki K
  • Journal Title: Molecular and Cellular Endocrinology 283 Pages: 1-11
• [Journal Article] The Involvement of Phosphatidylinositol 3-Kinase in Gonadotropin- Releasing Hormone Induced Gonadotropin α- and FSHβ Subunit Gene Expression in Clonal Gonadotroph LβT2 Cells (2008)
  • Author(s): Mutiara S, Kanasaki H., et.al
  • Journal Title: Molecular and Cellular Endocrinology 283 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Up-regulation of gonadotropin α-subunit gene by phosphatidylinositol 3- kinase inhibitors in clonal gonadotroph cells (2008)
  • Author(s): Kanasaki H, Mutiara S., et.al.
  • Journal Title: Neuroendocrinology Letters 29 Pages: 529-535
  • Peer Reviewed
• [Journal Article] Activation of Extracellular Signal-Regulated Kinase (ERK) and Induction of Mitogen-Activated Protein Kinase Phosphatase 1 (MKP1) by Perifused Thyrotropin-Releasing Hormone (TRH) Stimulation in Rat Pituitary GH3 Cells (2008)
  • Author(s): Oride A, Kanasaki H., et.al.
  • Journal Title: Molecular and Cellular Endocrinology 296 Pages: 78-86
  • Peer Reviewed
• [Journal Article] Paracrine control of gonadotrophs by somatolactotrophs through TRH-induced follistatin production.
  • Author(s): Kanasaki H, Oride A, Miyazaki K.
  • Journal Title: The Open Neuroendocrinology Journal in press Pages: 2011-2011
• [Presentation] ゴナドトロピン産生細胞におけるGnRHとPACAPの相互作用について (2010)
  • Author(s): 金崎春彦、折出亜希、インドリプルワナ、石川雅子、今村加代、片桐敦子、山上育子、中山健太郎、青木昭和、宮崎康二
  • Organizer: 第62回日本産科婦人科学会学術講演会
  • Place of Presentation: 東京
  • Year and Date: 2010-04-23
• [Presentation] GnRHパルス頻度依存性ゴナドトロピン産生・分泌調節における細胞内情報伝達機構 (2009)
  • Author(s): 金崎春彦
  • Organizer: 第82回日本内分泌学会総会 公募シンポジウム
  • Place of Presentation: 前橋市
  • Year and Date: 2009-04-23
• [Presentation] プロラクチン産生細胞におけるフォリスタチン発現とゴナドトロピン合成に対する影響について (2009)
  • Author(s): 金崎春彦、折出亜希、インドリプルワナ、宮崎康二
  • Organizer: 第61回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都市
  • Year and Date: 2009-04-03
• [Presentation] 中枢神経系に存在するPituitary Adenylate Cyclase-Activating Polypeptide (PACAP)によるゴナドトロピンの産生調節シンポジウム2(中枢神経関連物質の生殖機能へのかかわり) (2009)
  • Author(s): 金崎春彦
  • Organizer: 第61回日本産科婦人科学会学術講演会
  • Place of Presentation: 京都市
  • Year and Date: 2009-04-03
• [Presentation] ゴナドトロピン産生細胞におけるPACAPの役割について (2008)
  • Author(s): 金崎春彦、原田崇、サンドラムティアラ、折出亜希、宮崎康二
  • Organizer: 第60回日本産科婦人科学会学術講演会
  • Place of Presentation: 横浜市
  • Year and Date: 2008-04-12