A Study of Dentin Regeneration via Mouse Embryonic Stem Cells
| Project/Area Number |
20791406
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Aichi Gakuin University |
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Principal Investigator |
OZEKI Nobuaki Aichi Gakuin University, 歯学部, 助教 (70469005)
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| Project Period (FY) |
2008 – 2009
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| Project Status |
Completed (Fiscal Year 2009)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 歯内療法学 / 移植・再生医療 / ES細胞 / 移植・再年医療 |
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| Research Abstract |
We have examined the mechanism of mouse embryonic stem cells differentiation to odontoblasts for the purpose of dentin regeneration. Morphological analysis indicated that retinoic acid (RA) and bone morphogenetic protein-4 (BMP-4) induced differentiation to the odontogenic pathway. Differentiated cells showed DSPP and DSP ; both markers of odontoblast differentiation. Moreover, we found significant integrin α2β1, α6β1 and αVβ1 expression with increased adhesion and motility on laminin-1 and collagen type-I substrates. These interactions with the extracellular matrix (ECM) are indicative of dental pulp wound healing.
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Report
(3 results)
Research Products
(2 results)
