A Study of Dentin Regeneration via Mouse Embryonic Stem Cells

Research Project

Project/Area Number	20791406
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Conservative dentistry
Research Institution	Aichi Gakuin University
Principal Investigator	OZEKI Nobuaki Aichi Gakuin University, 歯学部, 助教 (70469005)
Project Period (FY)	2008 – 2009
Project Status	Completed (Fiscal Year 2009)
Budget Amount *help	¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000) Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	歯内療法学 / 移植・再生医療 / ES細胞 / 移植・再年医療
Research Abstract	We have examined the mechanism of mouse embryonic stem cells differentiation to odontoblasts for the purpose of dentin regeneration. Morphological analysis indicated that retinoic acid (RA) and bone morphogenetic protein-4 (BMP-4) induced differentiation to the odontogenic pathway. Differentiated cells showed DSPP and DSP ; both markers of odontoblast differentiation. Moreover, we found significant integrin α2β1, α6β1 and αVβ1 expression with increased adhesion and motility on laminin-1 and collagen type-I substrates. These interactions with the extracellular matrix (ECM) are indicative of dental pulp wound healing.

Report

(3 results)