Analysis of Wnt5a for alveolar bone resorption by periodontal disease.

• Project/Area Number: 20791624
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Matsumoto Dental University
• Principal Investigator: ISHIHARA Akihiro Matsumoto Dental University, 歯学部, 助手 (90460419)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 破骨細胞 / Wnt / 非古典経路

Research Abstract

This study demonstrated that Wnt5a enhances RANK ligand-induced osteoclast differentiation and resorption pit formation through the receptor Ror2 in osteoclast precursors and mature osteoclast, respectively. RT-PCR analysis showed that Wnt5a mRNA was strongly expressed in both osteoblasts and osteoclasts. RANK ligand-stimulated osteoclast formation and pit formation were enhanced by the treatment with Wnt5a. This enhancement effect of Wnt5a on osteoclast formation was dose-dependently suppressed by sRor2. sRor2 also inhibited the pit-forming activity of osteoclasts in a dose-dependent manner. These results suggest that GST-sRor2 acts as a decoy receptor of Wnt5a. Wnt5a represents a novel therapeutic target for periodontal disease.

Research Products

• [Journal Article] Multiwalled carbon nanotubes specifically inhibit osteoclast differentiation and function. (2009)
  • Author(s): Narita N, Kobayashi Y, Nakamura H, Maeda K, Ishihara A, Mizoguchi T, Usui Y, Aoki K, Simizu M, Kato H, Ozawa H, Udagawa N, Endo M, Takahashi N, Saito N.
  • Journal Title: Nano Lett 9(4) Pages: 1406-13
  • Peer Reviewed
• [Journal Article] Multi-walled carbon nanotubes specifically inhibit osteoclast differentiation and function (2009)
  • Author(s): Nobuyo Narita
  • Journal Title: Nano Letters (印刷中)
  • Peer Reviewed
• [Presentation] Roles of Wnt5a-Ror2 signaling in Osteoclast function (2010)
  • Author(s): Ishihara A, Kobayashi Y, Maeda K, Uehara S, Yoshinari N, Kato S, Minami Y, Udagawa N, Takahashi N
  • Organizer: Network for International Education and Research in Advanced Dental Sciences
  • Place of Presentation: Niigata
  • Year and Date: 2010-02-10
• [Presentation] 破骨細胞の骨吸収活性におけるWnt5a-Ror2シグナルの役割について (2009)
  • Author(s): 石原章弘
  • Organizer: 先端歯学スクール2009
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫県)
  • Year and Date: 2009-10-20
• [Presentation] 破骨細胞の骨吸収活性におけるWnt5a-Ror2シグナルの役割について (2009)
  • Author(s): 石原章弘
  • Organizer: 先端歯学スクール2009
  • Place of Presentation: 兵庫
  • Year and Date: 2009-08-28
• [Presentation] Wnt5aは生理的および病的破骨細胞形成に必要である (2008)
  • Author(s): 前田和洋, 小林泰浩, 石原章弘, 宇田川信之, 高田伊知郎, 加藤茂明, 丸毛啓史, 高橋直之
  • Organizer: 第26回日本骨代謝学会プログラム抄録集:p. 228)日本骨代謝学会(第26回)
  • Year and Date: 2008-10-31
• [Presentation] Wnt5aは生理的および病的破骨細胞形成に必要である (2008)
  • Author(s): 前田和洋
  • Organizer: 第26回日本骨代謝学会
  • Place of Presentation: 大阪(大阪国際会議場)
  • Year and Date: 2008-10-31
• [Presentation] Wnt5a secreted by osteoblasts regulates osteoclast differentiation (2008)
  • Author(s): Maeda K, Kobayashi Y, Ishihara A, Udagawa N, Takada I, Kato S, Nishita M, Minami Y, Marumo K, Takahashi N
  • Organizer: 2nd International Conference on Osteoimmunology
  • Year and Date: 2008-06-09
• [Remarks]
  • URL: http://www.mdu.ac.jp/laboratory/research_contents/index.html