Study of the microRNA pathway controlling developmental timing : Utilization of the amyloid precursor protein-like gene

• Project/Area Number: 20870005
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: University of Tsukuba
• Principal Investigator: NIWA Ryusuke University of Tsukuba, 大学院・生命環境科学研究科, 助教 (60507945)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 発生・分化 / 発現制御 / 線虫 / 発生タイミング / マイクロRNA / 転写因子 / 核内受容体

Research Abstract

The development of multicellular organisms is tightly regulated under a precise time schedule. To unravel the molecular mechanisms underlying developmental timing in multicellular organisms, I used the nematode Caenorhabditis elegans as the model organism. I identified and characterized genes involved in the let-7 microRNA pathway, which is essential for the larva-to-adult transition. I found that the nuclear receptor gene nhr-25 plays a crucial role in the developmental fate switch from larval to adult stages in C.elegans.

Research Products

• [Journal Article] The nuclear receptor gene nhr-25 plays multiple roles in the C. elegans heterochronic gene network to control the larva-to-adult transition (2010)
  • Author(s): Kazumasa Hada, Masako Asahina, Hiroshi Hasegawa, Yasunori Kanaho, Frank J. Slack, Ryusuke Niwa
  • Journal Title: Developmental Biology (掲載決定済み)
• [Presentation] 脱皮動物の発生タイミングの分子機構 : 昆虫エクジソン生合成経路および線虫ヘテロクロニック遺伝子の研究を通じて (2009)
  • Author(s): 丹羽隆介
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] 脱皮動物の発生タイミングの分子機構:昆虫エクジソン生合成経路および線虫ヘテロクロニック遺伝子の研究を通じて (2009)
  • Author(s): 丹羽隆介
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-12
• [Presentation] A screen for novel heterochronic genes that regulate the let-7 microRNA-dependent developmental timing pathway in the nematode Caenorhabditis elegans (2009)
  • Author(s): Kazumasa Hada, Sora Enya, Ryusuke Niwa
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] A screen for novel heterochronic genes that regulate the let-7 microRNA-dependent developmental timing pathway in the nematode Caenorhabditis elegans (2009)
  • Author(s): Kazumasa Hada, Sora Enya, Ryusuke Niwa
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] Hunting for new heterochronic genes that play essential roles in the let-7 microRNA-dependent pathway in Caenorhabditis elegans (2009)
  • Author(s): Kazumasa Hada, Sora Enya, Ryusuke Niwa
  • Organizer: International Symposium on Cellular Signaling ～principles and functions～
  • Place of Presentation: 筑波大学
  • Year and Date: 2009-11-19
• [Presentation] The nuclear hormone receptor nhr-25 is a heterochronic gene that has dual roles in both promoting and inhibiting C.elegans adult programs (2009)
  • Author(s): Ryusuke Niwa, Kazumasa Hada, Hiroshi Hasegawa, Masako Asahina, Yasunori Kanaho, Frank J.Slack
  • Organizer: The 17th International C.elegans Meeting
  • Place of Presentation: カリフォルニア州立大学ロサンゼルス校(米国)
  • Year and Date: 2009-06-26
• [Presentation] The nuclear hormone receptor nhr-25 is a heterochronic gene that has dual roles in both promoting and inhibiting C. elegans adult programs (2009)
  • Author(s): Ryusuke Niwa, Kazumasa Hada, Hiroshi Hasegawa, Masako Asahina, Yasunori Kanaho, Frank J. Slack
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: カリフォルニア大学ロサンゼルス校、アメリカ合衆国
  • Year and Date: 2009-06-24
• [Presentation] Identification and characterization of novel heterochronic genes involved in the let-7 microRNA-dependent developmental timing pathway in Caenorhabditis elegans (2009)
  • Author(s): Kazumasa Hada, Hiroshi Hasegawa, Yasunori Kanaho, Frank J. Slack, Ryusuke Niwa
  • Organizer: 17th International C. elegans Meeting
  • Place of Presentation: カリフォルニア大学ロサンゼルス校、アメリカ合衆国
  • Year and Date: 2009-06-24
• [Presentation] Identification and characterization of novel heterochronic genes involved in the let-7 microRNA-dependent developmental timing pathway in Caenorhabditis elegans (2009)
  • Author(s): Kazumasa Hada, Hiroshi Hasegawa, Yasunori Kanaho, Ryusuke Niwa
  • Organizer: 日本発生生物学会第42回大会
  • Place of Presentation: 新潟コンベンションセンター朱鷺メッセ
  • Year and Date: 2009-05-28
• [Presentation] 線虫 C. elegans の let-7 microRNA 発生タイミング経路に関わる新規遺伝子の同定と機能解析 (2009)
  • Author(s): 波田一誠, 長谷川潤, 金保安則, Frank J. Slack, 丹羽隆介
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: マリンメッセ福岡
  • Year and Date: 2009-03-27
• [Presentation] Identification and characterization of novel heterochronic genes involved in the let-7 microRNA-dependent developmental timing pathway in Caenorhabditis elegans (2009)
  • Author(s): Kazumasa Hada, Hiroshi Hasegawa, Yasunori Kanaho, Ryusuke Niwa
  • Organizer: The 42nd Annual meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 朱鷺メッセ(新潟市)
• [Presentation] Identification and characterization of novel heterochronic genes involved in the let-7 microRNA-dependent developmental timing pathway in Caenorhabditis elegans (2009)
  • Author(s): Kazumasa Hada, Hiroshi Hasegawa, Yasunori Kanaho, Frank J.Slack, Ryusuke Niwa
  • Organizer: The 17th International C.elegans Meeting
  • Place of Presentation: カリフォルニア州立大学ロサンゼルス校(米国)
• [Presentation] Hunting for new heterochronic genes that play essential roles in the let-7 microRNA-dependent pathway in Caenorhabditis elegans (2009)
  • Author(s): Kazumasa Hada, Sora Enya, Ryusuke Niwa
  • Organizer: International Symposium on Cellular Signaling～principles and functions～
  • Place of Presentation: 筑波大学
• [Presentation] 線虫C. elegansのlet-7 microRNA発生タイミング経路に関わる新規遺伝子の同定と機能解析 (2009)
  • Author(s): 波田一誠、長谷川潤、金保安則、Frank J. Slack、丹羽隆介
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: マリンメッセ福岡
• [Remarks] ホームページ
  • URL: http://www.biol.tsukuba.ac.jp/~rniwa/
• [Remarks]
  • URL: http://www.biol.tsukuba.ac.jp/~rniwa
• [Remarks]
  • URL: http://www.biol.tsukuba.acjp/~rniwa