Analyses of a novel cell shape determinant in rod-shaped bacterium Escherichia coli.

• Project/Area Number: 20870039
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: National Institute of Genetics
• Principal Investigator: SHIOMI Daisuke National Institute of Genetics, 系統生物研究センター, 研究員 (70507532)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 大腸菌 / 形態形成 / 細胞骨格 / 蛍光タンパク質 / 細胞膜 / ペプチドグリカン / 次世代ゲノムシークエンス / 抑圧変異体 / 細胞生物学 / 微生物

Research Abstract

I identified a novel cytoskeletal membrane protein RodZ which regulates cell shape in rod-shaped bacterium E.coli. Cells lacking rodZ are round. It was suggested that RodZ regulates the cell length. Analyses of suppressor mutants of cells lacking rodZ revealed genetic interactions between RodZ and MreB (actin) or cell division machinery that includes FtsZ (tubulin), suggesting that all three cytoskeletal proteins in E.coli collaborate to determine cell shape.

Research Products

• [Journal Article] Genetic mechanism regulating bacterial cell shape and metabolism. (2009)
  • Author(s): Shiomi D, Mori H, Niki H
  • Journal Title: Communicative & Integrative Biology 2(3) Pages: 219-220
• [Journal Article] 細胞骨格タンパク質による大腸菌の形態形成機構 (2009)
  • Author(s): 塩見大輔、仁木宏典
  • Journal Title: 日本微生物生態学会誌 24(2) Pages: 51-60
  • NAID: 110007356220
• [Journal Article] Genetic mechanism regulating bacterial cell shape and metabolism (2009)
  • Author(s): 塩見大輔
  • Journal Title: Communicative & Integrative Biology 2 Pages: 219-220
• [Journal Article] 細胞骨格タンパク質による大腸菌の形態形成機構 (2009)
  • Author(s): 塩見大輔
  • Journal Title: 日本微生物生態学会誌 24 Pages: 51-60
  • NAID: 110007356220
• [Journal Article] Determination of bacterial rod shape by a novel cytoskeletal membrane protein. (2008)
  • Author(s): Shiomi D, Sakai M, Niki H
  • Journal Title: EMBO J 27(23) Pages: 3081-3091
  • Peer Reviewed
• [Journal Article] Determination of bacterial rod shape by a novel cytoskeietal membrane protein. (2008)
  • Author(s): Daisuke Shiomi
  • Journal Title: The EMBO Journal 27 Pages: 3081-3091
  • Peer Reviewed
• [Presentation] Genetic interactions between proteins that govern rod-shape of E. coli (2011)
  • Author(s): 塩見大輔、仁木宏典
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] lobal regulatory network among proteins that determine cell shape in E. coli. (2010)
  • Author(s): 塩見大輔、仁木宏典
  • Organizer: 第32回日本分子生物学会年会ワークショップ「原核生物で新たに見出された細胞複製の基本分子機構」
  • Place of Presentation: パシフィコ横浜
• [Presentation] B. sunbtilis knockout strains by the National BioResource Project in Japan (2010)
  • Author(s): Daisuke Shiomi, Keita Aoki, Hironori Niki
  • Organizer: 5th Conference on Functional Genomics of Gram-positive Microorganism San Diego
  • Place of Presentation: CA, USA
• [Presentation] 細胞骨格タンパク質RodZの抑制変異解析から見た形態形成の制御の遺伝子ネットワーク (2010)
  • Author(s): 塩見大輔、仁木宏典
  • Organizer: 第6回21世紀大腸菌研究会
  • Place of Presentation: KKR熱海
• [Presentation] 桿菌の形を決める新規の細胞骨格性タンパク質 (2010)
  • Author(s): 塩見大輔、境雅子、仁木宏典
  • Organizer: 第82回日本細菌学会総会シンポジウム「原核細胞の形と位置を決める仕組み」
  • Place of Presentation: 名古屋国際会議場
• [Presentation] 大腸菌の形態形成を支配する因子間の遺伝的相互作用 (2010)
  • Author(s): 塩見大輔
  • Organizer: 日本細菌学会総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 大腸菌の形態形成を司る新規因子の解析 (2009)
  • Author(s): 塩見大輔、境雅子、仁木宏典
  • Organizer: 第5回21世紀大腸菌研究会
  • Place of Presentation: 藤枝エミナース
• [Presentation] Novel cell shape determinant in Escherichia coli (2009)
  • Author(s): Daisuke Shiomi, Hironori Niki
  • Organizer: Gordon Conference“Bacterial Cell Surface"New London
  • Place of Presentation: NH, USA
• [Presentation] 細胞骨格タンパク質RodZの抑圧変異解析から見た形態形成制御の遺伝子ネットワーク (2009)
  • Author(s): 塩見大輔
  • Organizer: 21世紀大腸菌研究会
  • Place of Presentation: KKR熱海
• [Presentation] B.subtilis knockout strains by the National BioResource Project in Japan (2009)
  • Author(s): 塩見大輔
  • Organizer: International Conference on Gram-Positive Microorganisms
  • Place of Presentation: San Diego, CA(USA)
• [Presentation] Global regulatory network among proteins that determine cell shape in E.coli (2009)
  • Author(s): 塩見大輔
  • Organizer: 日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] 桿菌の形を決める新規の細胞骨格性タンパク質 (2009)
  • Author(s): 塩見大輔
  • Organizer: 日本細菌学会
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Novel cell shape determinant in Escherichia coli (2008)
  • Author(s): 塩見大輔
  • Organizer: Gordon Research Conference [Bacterial Cell Surface]
  • Place of Presentation: Colby-Sawyer College New London, NH, USA
• [Presentation] 大腸菌の形態形成を司る新規因子の解析 (2008)
  • Author(s): 塩見大輔
  • Organizer: 21世紀大腸菌研究会
  • Place of Presentation: 藤枝エミナース