| Project/Area Number |
20890143
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| Research Category |
Grant-in-Aid for Young Scientists (Start-up)
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| Allocation Type | Single-year Grants |
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| Research Field |
Periodontal dentistry
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| Research Institution | Hiroshima University |
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Principal Investigator |
YAMASHITA Akiko Hiroshima University, 医歯薬学総合研究科, 助教 (70511319)
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| Project Period (FY) |
2008 – 2009
|
| Project Status |
Completed (Fiscal Year 2009)
|
| Budget Amount *help |
¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
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| Keywords | アディポサイトカイン / 歯周病 / メタボリックシンドローム |
|---|
| Research Abstract |
We analyzed genes differentially expressed in adipocytes when co-cultured with macrophages in the presence of a ligand TLR-4, bacterial lipopolysaccharide (LPS). RAW264.7, a murine macrophage cell line and differentiated 3T3-L1 adipocytes were co-cultured using a transwell system. Genes differentially expressed in adipocytes were analyzed by the DNA microarray method. Co-culture of macrophages and adipocytes with a low LPS concentration markedly upregulated gene expressions associated with inflammation and/or angiogenesis, such as those of IL-6, MCP-1, RANTES and CXCL1/KC, in adipocytes.
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