Regulation of membrane trafficking in synaptic formation and maturation.

• Project/Area Number: 20890149
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: The University of Tokushima
• Principal Investigator: SAKANE Ayuko The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 助教 (60509777)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 高次神経機能 / シナプス形成 / シナプス成熟 / 小胞輸送 / Rabファミリー低分子量G蛋白質 / シナプス形戌

Research Abstract

The Rab family of small G proteins, which consists of more than 60 different members in mammalian cells, is a key regulator in membrane trafficking. Since each member recognizes distinct subsets on intracellular membranes, this family may contribute to temporal and spatial regulation of signal transduction in various cellular functions. In this project, we analyzed the functions of Rab proteins in synaptic formation and following maturation.

Research Products

• [Journal Article] Rab13 regulates neurite outgrowth in PC12 cells through its effector protein (2010)
  • Author(s): Sakane A., Honda K., Sasaki, T.
  • Journal Title: JRAB/MICAL-L2. Mol. Cell Biol. 30(4) Pages: 1077-1087
• [Journal Article] Rab13 regulates neurite outgrowth in PC12 cells through its effector protein, JRAB/MICAL-L2. (2010)
  • Author(s): Sakane A., et al.
  • Journal Title: Mol.Cell.Biol. 30 Pages: 1077-1087
  • Peer Reviewed
• [Journal Article] APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle. (2009)
  • Author(s): Szodorai, A., Kuan, Y-H., Hunzelmann, S., Engel, U., Sakane, A., Sasaki, T., Takai Y., Kirsch, J., Moller, U., Beyreuther, K., Brady, S., Morfini, G., Kins, S.
  • Journal Title: J. Neurosci. 29(46) Pages: 14534-14544
• [Journal Article] 佐々木卓也RabファミリーSmallGタンパク質による小胞輸送制御と高次生命機能 (2009)
  • Author(s): 坂根亜由子
  • Journal Title: 細胞工学 28巻12号 Pages: 1247-1251
• [Journal Article] APP anterograde transport requires Rab3A GTPase activity for assembly of the transport vesicle. (2009)
  • Author(s): Szodorai, A., et al.
  • Journal Title: J.Neurosci. 29 Pages: 14534-14544
  • Peer Reviewed
• [Journal Article] RabファミリーSmall Gタンパク質による小胞輸送制御と高次生命機能 (2009)
  • Author(s): 坂根亜由子, 他
  • Journal Title: 細胞工学 28巻 Pages: 1247-1251
• [Journal Article] Analysis on the emerging role of Rab3 GTPase-activating protein in Warburg Micro and Martsolf syndrome. (2008)
  • Author(s): Sakane. A., Miyoshi, S., Takai, Y., Sasaki, T.
  • Journal Title: Methods Enzyntol. 438 Pages: 131-139
• [Journal Article] Doc2αand Munc13-4 regulate Ca2+-dependent secretory lysosome exocytosis in mast cells. (2008)
  • Author(s): Higashio, H., Nishimura, N., Ishizaki, H., Miyoshi, J., Orita, S., Sakane, A., Sasaki, T.
  • Journal Title: J. Immunol. 180(7) Pages: 4774-4784
• [Journal Article] 佐々木卓也高次機能システムを支えるエキソサイトーシス系Rabファミリー低分子量 (2008)
  • Author(s): 坂根亜由子
  • Journal Title: GTPase蛋白質核酸酵素 53巻16号 Pages: 2130-2135
• [Journal Article] Doc2α and Munc13-4 regulate Ca^<2+>-dependent secretory lysosome exocytosis in mast cells. (2008)
  • Author(s): Higashio, H., et al.
  • Journal Title: J. Immunol. 180 Pages: 4774-4784
  • Peer Reviewed
• [Journal Article] Analysis on the emerging role of Rab3 GTPase-activating protein in Warburg Micro and Martsolf syndrome. (2008)
  • Author(s): Sakane, A., et al.
  • Journal Title: Methods Enzymol. 438 Pages: 131-139
  • Peer Reviewed
• [Journal Article] 高次機能システムを支えるエキソサイトーシス系Rabファミリー低分子量GTPase (2008)
  • Author(s): 坂根亜由子, 他
  • Journal Title: 蛋白質核酸酵素 53 Pages: 2130-2135
• [Presentation] Exocytotic Rab small G proteins regulate neuronal development and plasticity. (2008)
  • Author(s): Takuya Sasaki, Ayuko Sakane
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] Exocytotic Rab small G proteins regulate neuronal development and plasticity. (2008)
  • Author(s): Takuya Sasaki, Ayuko Sakane
  • Organizer: BMB2008(第31回日本分子生物学会年会・第81回日本生化学会大会合同大会)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-11
• [Presentation] 高次細胞機能発現におけるエキソサイトーシス系Rabファミリー低分子量G蛋白質の作用機構 (2008)
  • Author(s): 佐々木卓也, 西村範行, 坂根亜由子
  • Organizer: 第60回日本細胞生物学会大会
  • Place of Presentation: 横浜
  • Year and Date: 2008-06-30