Immune regulatory effect of C-type lectin DC-SIGN on colorectal tumors

• Project/Area Number: 20890255
• Research Category: Grant-in-Aid for Young Scientists (Start-up)
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Ritsumeikan University
• Principal Investigator: NONAKA Motohiro Ritsumeikan University, 総合理工学研究機構, 研究員 (70514173)
• Project Period (FY): 2008 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥3,302,000 (Direct Cost: ¥2,540,000、Indirect Cost: ¥762,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,742,000 (Direct Cost: ¥1,340,000、Indirect Cost: ¥402,000)
• Keywords: 樹状細胞 / 結腸がん / 免疫制御 / DC-SIGN / MBP / DNA / ファゴサイトーシス / アポトーシス / Jacalin / B細胞 / CD45

Research Abstract

We found that C-type lectin DC-SIGN expressed on dendritic cells recognized colorectal tumor-associated Lewis glycans expressed on colorectal carcinoma COLO205 cells, which contributed to tolerogenic immunity for tumor cells. We identified DC-SIGN ligands which were involved in the interaction with DC-SIGN. Moreover, we revealed that mannan binding protein interacted with DNA derived from various orignins in a calcium dependent manner.

Research Products

• [Journal Article] Colorectal carcinoma-associated carbohydrate recognition and tumor-immunological regulation by C-type lectin DC-SIGN (2009)
  • Author(s): Motohiro Nonaka、Bruce Yong Ma, Toshisuke Kawasaki
  • Journal Title: Trends in Glycoscience and Glycotechnology 第21巻、第120号 Pages: 228-236
  • NAID: 10026976814
• [Journal Article] Characterization of the interaction between serum mannan-binding protein and nucleic acid ligands (2009)
  • Author(s): Natsuko Nakamura*、Motohiro Nonaka*、Bruce Yong Ma*、Shogo Matsumoto、Nobuko Kawasaki、Shinji Asano, Toshisuke Kawasaki
  • Journal Title: Journal of Leukocyte Biology 第86巻 Pages: 737-748
  • Peer Reviewed
• [Journal Article] The lectin Jacalin induces human B lymphocyte apoptosis through glycosylation-dependent interaction with CD45 (2009)
  • Author(s): Bruce Yong Ma、Kaori Yoshida、Makoto Baba、Motohiro Nonaka、Shogo Matsumoto、Nobuko Kawasaki、Shinji Asano, Toshisuke Kawasaki
  • Journal Title: Immunology 第127巻 Pages: 477-488
  • Peer Reviewed
• [Journal Article] Characterization of the interaction between serum mannan-binding protein and nucleic acid ligands (2009)
  • Author(s): 中村奈都子、野中元裕、Ma Bruce Yong、松本尚悟、川嵜伸子、浅野真司、川嵜敏祐
  • Journal Title: Journal of Leukocyte Biology Vol.86 Pages: 737-748
  • Peer Reviewed
• [Journal Article] Colorectal carcinoma-associated carbohydrate recognition and tumor-immunological regulation by C-type lectin DC-SIGN (2009)
  • Author(s): 野中元裕、Ma Bruce Yong、川嵜敏祐
  • Journal Title: Trends in Glycoscience and Glycotechnology Vol.21 Pages: 228-236
  • NAID: 10026976814
• [Journal Article] (2009)
  • Author(s): 野中元裕、Ma Bruce Yong、川嵜敏祐
  • Journal Title: 遺伝子医学MOOK別冊「ますます重要になる細胞周辺環境(細胞ニッチ)」(メディカル ドゥ) Pages: 257-262
• [Journal Article] The lectin Jacalin induces human B lymphocyte apoptosis through glycosylation-dependent interaction with CD45 (2009)
  • Author(s): Bruce Yong Ma
  • Journal Title: Immunology 127 Pages: 477-488
  • Peer Reviewed
• [Presentation] Characteristic interaction of serum mannan-binding protein with nucleic acids (2009)
  • Author(s): Motohiro Nonaka、Bruce Yong Ma、Shogo Matsumoto、Nobuko Kawasaki, Toshisuke Kawasaki
  • Organizer: 2009 Annual Meeting of the Society for Glycobiology
  • Place of Presentation: San Diego(USA)
  • Year and Date: 2009-11-13
• [Presentation] Characteristic interaction of serum mannan-binding protein with nucleicacids (2009)
  • Author(s): 野中元裕、Ma Bruce Yong、松本尚悟、川嵜伸子、川嵜敏祐
  • Organizer: 2009 Annual Meeting of the Society for Glycobiology
  • Place of Presentation: サンディエゴ(米国)
  • Year and Date: 2009-11-13
• [Presentation] 血清型マンナン結合タンパク質と核酸の相互作用の解析とその生物学的意義 (2009)
  • Author(s): 野中元裕, Ma Bruce Yong, 松本尚悟, 川嵜伸子, 川嵜敏祐
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2009-10-24
• [Presentation] 血清型マンナン結合タンパク質による核酸の認識とその意義 (2009)
  • Author(s): 野中元裕, Ma Bruce Yong, 中村奈都子, 松本尚悟, 川嵜伸子, 川嵜敏祐
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 飛騨・世界生活文化センター(岐阜県)
  • Year and Date: 2009-09-11
• [Presentation] Characteristic recognition and biological significance of the interaction between serum mannan-binding protein and nucleic acids (2009)
  • Author(s): Motohiro Nonaka, Bruce Yong Ma, Shogo Matsumoto, Nobuko Kawasaki, Toshisuke Kawasaki
  • Organizer: 21st IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Shanghai, (China)
• [Presentation] Characteristic recognition and biological significance of the interaction between serum mannan-binding protein and nucleic acids (2009)
  • Author(s): 野中元裕、Ma Bruce Yong、松本尚悟、川嵜伸子、川嵜敏祐
  • Organizer: 21st IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 上海(中国)
• [Presentation] 樹状細胞C型レクチンDC-SIGNによる結腸がん細胞表面糖鎖の認識と腫瘍免疫の調節機構 (2008)
  • Author(s): 野中元裕, Ma BY, 川嵜伸子, 川嵜敏祐
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸国際展示場(兵庫県)
  • Year and Date: 2008-12-12
• [Presentation] 樹状細胞C型レクチンDC-SIGNによる結腸がん細胞表面糖鎖の認識と腫瘍免疫の調節機構 (2008)
  • Author(s): 野中元裕
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会大会合同大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Presentation] レクチンJacalinによるBリンパ球のアポトーシスの誘導 (2008)
  • Author(s): Ma BY, 野中元裕, 川嵜敏祐
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2008-12-03
• [Presentation] レクチンJacalinによるBリンパ球のアポトーシスの誘導 (2008)
  • Author(s): Bruce Yong Ma
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Functional Recognition of Colorectal Tumor-associated Lewis Glycans by C-type Lectin DC-SIGN (2008)
  • Author(s): Motohiro Nonaka, Bruce Yong Ma, Nobuko Kawasaki, Toshisuke Kawasaki
  • Organizer: Annual Conference of the Society for Glycobiology
  • Place of Presentation: Texas(USA)
  • Year and Date: 2008-11-14
• [Presentation] Functional Recognition of Colorectal Tumor-associated Lewis Glycans by C-type Lectin DC-SIGN (2008)
  • Author(s): Motohiro Nonaka
  • Organizer: Annual Conference of the Society for Glycobiology
  • Place of Presentation: Texas、USA
  • Year and Date: 2008-11-14
• [Presentation] Jacalin-induced diverse immune responses through glycosylation-dependent interaction with CD45 on lymphocytes (2008)
  • Author(s): Motohiro Nonaka, Bruce Yong Ma, Nobuko Kawasaki, Toshisuke Kawasaki
  • Organizer: 8th Human Disease Glycomics/ Proteome Initiative (HGPI) meeting
  • Place of Presentation: Texas(USA)
  • Year and Date: 2008-11-13
• [Presentation] Jacalin-induced diverse immune responses through glycosylation-dependent interaction with CD45 on lymphocytes (2008)
  • Author(s): Motohiro Nonaka
  • Organizer: 8th Human Disease Glycomics/Proteome Initiative(HGPI)meeting
  • Place of Presentation: Texas、USA
  • Year and Date: 2008-11-13
• [Presentation] CD45を標的とするJacalinによるBリンパ球のアポトーシスの誘導 (2008)
  • Author(s): 松本尚悟, Ma BY, 野中元裕, 吉田香織, 馬場亮人, 川嵜伸子, 浅野真司, 川嵜敏祐
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば(茨城県)
  • Year and Date: 2008-08-19
• [Presentation] CD45を標的とするJacalinによるByンパ球のアポトーシスの誘導 (2008)
  • Author(s): 松本尚悟
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば(茨城県)
  • Year and Date: 2008-08-19
• [Presentation] Functional Implications for C-type Lectin DC-SIGN Recognizing Colorectal Tumor-Associated Lewis Glycans (2008)
  • Author(s): Motohiro Nonaka, Bruce Yong Ma, Nobuko Kawasaki, Toshisuke Kawasaki
  • Organizer: The 23rd International Lectin Conference (Interlec-23)
  • Place of Presentation: Edinburgh, (Scotland)
  • Year and Date: 2008-07-14
• [Book] 遺伝子医学MOOK別冊「ますます重要になる細胞周辺環境(細胞ニッチ)」 (2009)
  • Author(s): 野中元裕・Bruce Yong Ma・川嵜敏祐
  • Publisher: メディカルドゥ
• [Book] 糖鎖認識レセプターによる糖鎖シグナルの制御「遺伝子医学MOOK」 (2009)
  • Author(s): 野中元裕
  • Publisher: メディカルドゥ(印刷中)
• [Book] 蛋白質核酸酵素 (2008)
  • Author(s): 野中元裕, Bruce Yong Ma, 川嵜敏祐
  • Publisher: 共立出版
• [Book] 樹状細胞レクチンによる糖鎖認識と免疫調節「蛋白質核酸酵素」 (2008)
  • Author(s): 野中元裕
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.glyco2.is.ritsumei.ac.jp/RCG/
• [Remarks]
  • URL: http://www.glyco2.is.ritsumei.ac.jp/RCG/
• [Remarks]
  • URL: http://www.glyco2.is.ritsumei.ac.jp/RCG/