| Project/Area Number |
20F20361
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Review Section |
Basic Section 90110:Biomedical engineering-related
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| Research Institution | The University of Tokyo |
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| Host Researcher |
竹内 昌治 東京大学, 大学院情報理工学系研究科, 教授 (90343110)
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| Foreign Research Fellow |
MYASNIKOVA DINA 東京大学, 情報理工学(系)研究科, 外国人特別研究員
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| Project Period (FY) |
2020-11-13 – 2023-03-31
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| Project Status |
Granted (Fiscal Year 2021)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2022: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2021: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2020: ¥600,000 (Direct Cost: ¥600,000)
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| Keywords | skin / vascular structure / perfusion culture |
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| Outline of Research at the Start |
糖尿病患者の約5割が手足の感覚障害（末梢神経障害）を経験していると推定されている。しかし、この障害の正確なメカニズムや有効な治療法は未だ判明していない。要因として、最も多く用いられている本疾患のモデルがヒトとの類似性に欠けている複雑な動物モデルであったり、あまりにも単純なin vitroモデルであることがあげられる。
そこで本研究では、末梢神経障害の主要な構成要素である膵臓や皮膚を血管や神経細胞ネットワークに組み込んだ新しいin vitroモデルの確立を目指す。このモデルが実現すれば、選択された組織間の相互作用の解明に役立ち、糖尿病性末梢神経障害の研究を前進させる一助となることが期待できる。
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| Outline of Annual Research Achievements |
From 11.2020 until 04.2021 I mastered the culturing technique for fabrication of skin equivalent (SE) with correct architecture of 4 epidermis layers. I learnt the standard techniques for SE characterization (hematoxylin and eosin staining; measurement of TEER). Now, I am learning the HPLC to perform permeation test, which is an important for characterizing skin barrier function.
I designed a device for fabrication of SE with vascular structure. I optimized the SE culture protocol for the device and learnt perfusion culture technique. Now I am able to fabricate SE with perfusable channels.
Due to the coronavirus the purchasing of iPS cells took longer than I planned, but I am certain that I will be able to start part of the experiment connected with iPS cells from 05.2021.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
The laboratory has provided all necessary equipment. If some repair or guidance was needed it was provided quickly. Moreover, professor is very enthusiastic at improving research environment, so he helps with finding and choosing a better equipment for required experiment.
I would also like to mention that there are senior researches with a wide background range at the laboratory, who are always helping with setting up the experiment or provide valuable insights and comments to help with the experiment progress.
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| Strategy for Future Research Activity |
Next year goals are to optimizing the perfusion conditions of the SE. And incorporating neuron cells into the SE structure. The last step will require work with iPS cells and may take quite a bit of time, as the culture protocols take at least 1 month until differentiation is complete.
Another important step is to perform a permeation test, as it is vital to assess the SE barrier function and to test the applicability of my model for drug screening.
I will start working with pancreatic islet model and improve it by using iPS cells.
And the last goal of this year would be to fabricated ROS sensing cellulose beads. This will require some background literature work and design of the device for beads fabrication.
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