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Establishment of tasty fish strain using surrogate broodstock

Research Project

Project/Area Number 20H00430
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Review Section Medium-sized Section 40:Forestry and forest products science, applied aquatic science, and related fields
Research InstitutionTokyo University of Marine Science and Technology

Principal Investigator

Yoshizaki Goro  東京海洋大学, 学術研究院, 教授 (70281003)

Co-Investigator(Kenkyū-buntansha) 坂本 崇  東京海洋大学, 学術研究院, 教授 (40313390)
矢澤 良輔  東京海洋大学, 学術研究院, 准教授 (70625863)
壁谷 尚樹  東京海洋大学, 学術研究院, 助教 (90758731)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Fiscal Year 2022: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
Fiscal Year 2021: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2020: ¥16,900,000 (Direct Cost: ¥13,000,000、Indirect Cost: ¥3,900,000)
Keywords粗脂肪含量 / ニベ / 育種 / 生殖細胞 / 凍結保存 / 代理親魚 / 生殖細胞移植 / 脂肪 / 生殖幹細胞 / 代理新魚
Outline of Research at the Start

養殖魚では、その評価が侵襲的になされる形質に着目した育種はほとんど着手されていない。対象個体の評価に伴い、その個体を殺してしまう必要が生じる場合、その次世代を生産できないことがその大きな理由である。ゲノム情報を駆使して集団選抜を行うことも理論的には可能ではあるが、これには多大なスペースと労力、コストを必要とする。さらに、親世代の集団にエリート個体が含まれていなかった場合、その効果は限定的である。本申請では、可食部のサンプリングの際に未熟な生殖細胞を単離・凍結し、エリート個体を同定した後に、エリート個体の凍結生殖細胞を代理親魚へと移植することで、エリート個体に由来する卵、精子の生産を目指す。

Outline of Final Research Achievements

We tested the feasibility of breeding individuals with high total lipids using the marine fish, Nibe, as a model. First, the total lipids of wild individuals were analyzed, and at the same time, the gonads of these individuals were cryopreserved in liquid nitrogen. After identifying individuals with high and low total lipids, their germ cells were transplanted into infertile recipients. They were then allowed to mature and then subjected to mating experiments. The results showed that the next generation derived from the recipients, receiving germ cells of individuals with high total lipids showed significantly higher total lipids than the next generation derived from the germ cells of individuals with low total lipids. These results indicate that fish fat content is inherited, which has been conventionally thought to be greatly affected by diet.

Academic Significance and Societal Importance of the Research Achievements

従来、魚の脂の乗りは餌に大きく依存すると考えられてきたが、今回の研究で遺伝の影響も大きいことが明らかとなった。このことは、脂ののった魚を育種で生産可能であることを意味している。また、今回の研究では侵襲的な解析を行わないと評価できない表現型の育種が可能であることを明瞭に示すことができた。これにより、将来的には従来は未検討であった様々な新たな表現型に関する育種が進行することが期待される。

Report

(5 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Comments on the Screening Results   Annual Research Report
  • Research Products

    (67 results)

All 2022 2021 2020

All Journal Article (16 results) (of which Int'l Joint Research: 6 results,  Peer Reviewed: 16 results,  Open Access: 8 results) Presentation (49 results) (of which Int'l Joint Research: 8 results,  Invited: 16 results) Book (2 results)

  • [Journal Article] Production of albino chub mackerel (Scomber japonicus) by slc45a2 knockout and the use of a positive phototaxis-based larviculture technique to overcome the lethal albino phenotype2022

    • Author(s)
      Wataru Kawamura, Niki Hasegawa, Akihiro Yamauchi, Takashi Kimura, Hana Yahagi, Reoto Tani, Tetsuro Morita, Ryosuke Yazawa, Goro Yoshizaki
    • Journal Title

      Aquaculture

      Volume: 560 Pages: 738490-738490

    • DOI

      10.1016/j.aquaculture.2022.738490

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gametogenesis commencement in recipient gonads using germ cells retrieved from dead fish2022

    • Author(s)
      F Yang, K Ichida, G Yoshizaki.
    • Journal Title

      Aquaculture,

      Volume: 552 Pages: 737952-737952

    • DOI

      10.1016/j.aquaculture.2022.737952

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of Germ Cell-Less Rainbow Trout by dead end Gene Knockout and their Use as Recipients for Germ Cell Transplantation2022

    • Author(s)
      R Fujihara, N Katayama, Sadaie, S., Miwa, M., Sanchez Matias, G. A., Ichida, K., ... & Yoshizaki, G.
    • Journal Title

      Marine Biotechnology

      Volume: 24 Issue: 2 Pages: 417-429

    • DOI

      10.1007/s10126-022-10128-w

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of surrogate broodstock technology in Scombridae species by germ cell transplantation2022

    • Author(s)
      Reoto Tani,Ryosuke Yazawa,Shigeharu Kamio,Wataru Kawamura,Tetsuro Morita,Yutaka Takeuchi,Goro Yoshizaki
    • Journal Title

      Aquaculture Research

      Volume: 53 Issue: 7 Pages: 2760-2771

    • DOI

      10.1111/are.15791

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a polymerase chain reaction (PCR) based genetic sex identification method in the chub mackerel Scomber japonicus and blue mackerel S. australasicus2021

    • Author(s)
      Reoto Tani, Wataru Kawamura, Tetsuro Morita, Christophe Klopp, Marine Milhes, Yann Guiguen, Goro Yoshizaki, Ryosuke Yazawa
    • Journal Title

      Fisheries Science

      Volume: 87 Issue: 6 Pages: 785-793

    • DOI

      10.1007/s12562-021-01548-z

    • NAID

      40022746964

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Characterization of a vasa homolog in Mekong giant catfish (Pangasianodon gigas): Potential use as a germ cell marker.2021

    • Author(s)
      K Ichida, A Jangprai, P Khaosa-art, G Yoshizaki, S Boonanuntanasarn.
    • Journal Title

      Animal Reproduction Science

      Volume: 234 Pages: 106869-106869

    • DOI

      10.1016/j.anireprosci.2021.106869

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of a tracing technique for transplanted bluefin tuna germ cells in recipient’s gonads using monoclonal antibodies specifically recognizing bluefin tuna spermatogenic cells2021

    • Author(s)
      Yazawa Ryosuke、Kubokawa Tsubasa、Ichida Kensuke、Kawamura Wataru、Tani Reoto、Kamio Shigeharu、Morita Tetsuro、Yoshizaki Goro
    • Journal Title

      Fisheries Science

      Volume: 87 Issue: 1 Pages: 105-112

    • DOI

      10.1007/s12562-020-01486-2

    • NAID

      40022461817

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cryopreservation and Transplantation of Spermatogonial Stem Cells2021

    • Author(s)
      Zoran Marinovic、Jelena Lujic;、Qian Li、Yoshiko Iwasaki、Bela Urbanyi、Goro Yoshizaki、Akos Horvath
    • Journal Title

      Methods in Molecular Biology

      Volume: 2218 Pages: 37-47

    • DOI

      10.1007/978-1-0716-0970-5_4

    • ISBN
      9781071609699, 9781071609705
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Visualization and tracking of live type a spermatogonia using a fluorescence-conjugated antibody in Salmo species2021

    • Author(s)
      Ichida Kensuke、Matsushita Yoshiyuki、Amano Yuichi、Miwa Misako、Nagasawa Kazue、Hayashi Makoto、Mizutani Hanaka、Takahashi Momo、Boonanuntanasarn Surintorn、Yoshizaki Goro
    • Journal Title

      Aquaculture

      Volume: 533 Pages: 736096-736096

    • DOI

      10.1016/j.aquaculture.2020.736096

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Production of functional sperm from cryopreserved testicular germ cells following intraperitoneal transplantation into allogeneic surrogate in yellowtail (Seriola quinqueradiata)2021

    • Author(s)
      Morita Tetsuro、Miwa Misako、Kumakura Naoki、Morishima Kagayaki、Miki Takahisa、Takeuchi Yutaka、Yoshizaki Goro
    • Journal Title

      Cryobiology

      Volume: - Pages: 32-39

    • DOI

      10.1016/j.cryobiol.2021.04.001

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Oral administration of gonadotropin-releasing hormone analogue enhances previtellogenic oocyte growth of blue mackerel <i>Scomber australasicus</i>2020

    • Author(s)
      雨澤 孝太朗、尾崎 雄一、矢澤 良輔、竹内 裕、風藤 行紀、吉崎 悟朗
    • Journal Title

      NIPPON SUISAN GAKKAISHI

      Volume: 86 Issue: 2 Pages: 83-90

    • DOI

      10.2331/suisan.19-00029

    • NAID

      130007822580

    • ISSN
      0021-5392, 1349-998X
    • Year and Date
      2020-03-15
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of Chinese rosy bitterling offspring derived from frozen and vitrified whole testis by spermatogonial transplantation2020

    • Author(s)
      Octavera Anna、Yoshizaki Goro
    • Journal Title

      Fish Physiology and Biochemistry

      Volume: - Issue: 4 Pages: 1431-1442

    • DOI

      10.1007/s10695-020-00802-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Development of a simple method for sperm cryopreservation of Scombridae fishes in outdoor environments2020

    • Author(s)
      Kawamura Wataru、Yazawa Ryosuke、Tani Reoto、Takeuchi Yutaka、Morita Tetsuro、Yoshikawa Hiroyuki、Yoshizaki Goro
    • Journal Title

      Aquaculture Research

      Volume: 51 Issue: 8 Pages: 3376-3383

    • DOI

      10.1111/are.14673

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Production of functional eggs and sperm from in vitro-expanded type A spermatogonia in rainbow trout2020

    • Author(s)
      Iwasaki-Takahashi Yoshiko、Shikina Shinya、Watanabe Masaya、Banba Akira、Yagisawa Masaru、Takahashi Kasumi、Fujihara Ryo、Okabe Takafumi、Valdez Jr Delgado M.、Yamauchi Akihiro、Yoshizaki Goro
    • Journal Title

      Communications Biology

      Volume: 3 Issue: 1

    • DOI

      10.1038/s42003-020-1025-y

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Suitability of hybrid mackerel (Scomber australasicus?×?S. japonicus) with germ cell-less sterile gonads as a recipient for transplantation of bluefin tuna germ cells2020

    • Author(s)
      Kawamura Wataru、Tani Reoto、Yahagi Hana、Kamio Shigeharu、Morita Tetsuro、Takeuchi Yutaka、Yazawa Ryosuke、Yoshizaki Goro
    • Journal Title

      General and Comparative Endocrinology

      Volume: 295 Pages: 113525-113525

    • DOI

      10.1016/j.ygcen.2020.113525

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aging- and temperature-related activity of spermatogonial stem cells for germ cell transplantation in medaka2020

    • Author(s)
      Duangkaew Rungsun、Kezuka Fumi、Ichida Kensuke、Boonanuntanasarn Surintorn、Yoshizaki Goro
    • Journal Title

      Theriogenology

      Volume: 155 Pages: 213-221

    • DOI

      10.1016/j.theriogenology.2020.05.049

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] サバ科魚類の成熟期間を短縮するゼロ歳魚成熟誘導法の開発2022

    • Author(s)
      川村 亘、矢萩はな、矢澤良輔、森田哲朗、吉崎悟朗
    • Organizer
      第22回マリンバイオテクノロジー学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] DHA 合成能の付与を目指した海産魚脂肪酸代謝酵素の機能改変と CRISPR/Cas9 ノ ックイン技術によるゲノム編集2022

    • Author(s)
      松下芳之、木村隆志、壁谷尚樹、矢澤良輔、宇治督、吉崎悟朗
    • Organizer
      第22回マリンバイオテクノロジー学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] InDel マーカーを用いたマサバ、ゴマサバおよびその交雑魚の PCR による判別法の開発2022

    • Author(s)
      谷怜央人、川村亘、森田哲朗、吉崎悟朗、矢澤良輔
    • Organizer
      第22回マリンバイオテクノロジー学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Target mutagenesis of fads2 gene affects spermatogenesis and spawning success in2022

    • Author(s)
      Samaneh Poursaeid, Yosuke Akiba, Yoshiyuki Matsushita, Naoki Kabeya, Goro Yoshizaki
    • Organizer
      第22回マリンバイオテクノロジー学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 魚類の生殖細胞操作を利用した新たな養殖技法2022

    • Author(s)
      吉崎悟朗
    • Organizer
      第23回酵素応用シンポジウム
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] 黄体形成ホルモン遺伝子過剰発現ニジマス系統の生殖特性2022

    • Author(s)
      守谷 奈津子,三輪 美砂子,吉崎 悟朗
    • Organizer
      第115回日本繁殖生物学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 魚類におけるゲノム編集育種 の現状とその産業利用2022

    • Author(s)
      吉崎悟朗
    • Organizer
      一般社団法人日本育種学会 第142回講演会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Germ-line Stem Cell Manipulation in Fish: Can Mackerel Produce Bluefin Tuna Gametes?2022

    • Author(s)
      Goro yoshizaki
    • Organizer
      Frontiers in Stem Cells & Regeneration
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] DEVELOPMENT OF GERM CELL MANIPULATION TECHNOLOGY IN FISH2022

    • Author(s)
      Goro Yoshizaki, Ryosuke Yazawa
    • Organizer
      New Zealand Pland and Food Research Catalyst symposium
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Germ cell transplantation in fish: Production of bluefin tuna sperm using small surrogates2022

    • Author(s)
      吉崎悟朗
    • Organizer
      全能性と生殖細胞発生
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Germ cell manipulation in fish: Little tuna produces bluefin tuna sperm2022

    • Author(s)
      吉崎悟朗
    • Organizer
      第38回 国際生物学賞記念シンポジウム "魚の生物学:その生態、進化と発生"
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Dnd-knockout rainbow trout can produce Chinook salmon eggs and sperm within a short period of time.2022

    • Author(s)
      Goro Yoshizaki
    • Organizer
      2021 International Workshop: Perspective on Animal Science & International Exchange between SUT & SHINSHU
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] マサバにおけるゼロ歳魚成熟誘導法の開発 ~受精期の環境維持による早熟誘導~2022

    • Author(s)
      矢萩はな、川村亘、矢澤良輔、森田哲朗、吉崎悟朗
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] アンドロゲンの徐放的投与による ゼロ歳魚成熟マサバ精子生産の高効率化2022

    • Author(s)
      矢萩はな、川村亘、矢澤良輔、森田哲朗、吉崎悟朗
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] スマ雑種宿主が生産したドナー由来クロマグロ精子は 生殖幹細胞に由来するか?2022

    • Author(s)
      川村亘、天野雄一、矢澤良輔、森田哲朗、吉崎悟朗
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 天然記念物ミヤコタナゴをタイリクバラタナゴに産ませる2022

    • Author(s)
      山川宏樹、Anna Octavera、吉崎悟朗
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 黄体形成ホルモン遺伝子の未成魚における異所的過剰発現は 雄ニジマスを早熟化できるのか?2022

    • Author(s)
      守谷奈津子、三輪美砂子、吉崎悟朗
    • Organizer
      令和4年度日本水産学会春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Successful transplantation of donor spermatogonia derived from Postmortem rainbow trout2021

    • Author(s)
      Yang Fang, Kensuke Ichida, Goro Yoshizaki
    • Organizer
      第21回マリンバイオテクノロジー学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 生殖細胞欠損不妊海産魚の作出方法の検討2021

    • Author(s)
      山内章弘、長谷川二己、川村亘、矢澤良輔、吉崎悟朗
    • Organizer
      第21回マリンバイオテクノロジー学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 生殖細胞移植を用いたメコンオオナマズの配偶子生産2021

    • Author(s)
      市田健介、Rungsun Duangkaew、 Surintorn Boonanuntanasarn、吉崎悟朗
    • Organizer
      第21回マリンバイオテクノロジー学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 小型代理親魚が生産したドナー由来クロマグロ精子の濃縮法2021

    • Author(s)
      川村亘、神尾茂治、市田健介、矢澤良輔、森田哲郎、吉崎悟朗
    • Organizer
      第21回マリンバイオテクノロジー学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] アユの遺伝子資源保存に向けた生殖細胞凍結技術および移植技術の至適化2021

    • Author(s)
      天野雄一、鈴木弘貴、渡辺峻、阿久津崇、丸山瑠太、市田健介、 吉崎悟朗
    • Organizer
      第21回マリンバイオテクノロジー学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 生殖細胞操作を駆使した新たな育種法の開発2021

    • Author(s)
      吉崎悟朗
    • Organizer
      第21回マリンバイオテクノロジー学会大会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Production of functional eggs and sperm from in vitro-expanded type A spermatogonia in rainbow trout2021

    • Author(s)
      Goro Yoshizaki
    • Organizer
      Japan-Norway Symposium on Fish Endocrinology and Genomics symposium
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Production of functional gametes derived from cryopreserved spermatogonia via transplantation into recipients: a case study with endangered bitterlings.2021

    • Author(s)
      Goro Yoshizaki
    • Organizer
      CRYO2021 Virtual Conference - the 58th Annual Meeting of the Society for Cryobiology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 発生、繁殖制御技術を駆使した新たな育種法の開発2021

    • Author(s)
      吉崎 悟朗
    • Organizer
      シンポジウム「持続可能な次世代養殖システムの開発~サバを中心に~」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 培養生殖細胞からのニジマス個体の作出2021

    • Author(s)
      吉崎 悟朗
    • Organizer
      「認識と形成」研究会2021オンライン
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 黄体形成ホルモン遺伝子の未成魚における過剰発現は雄ニジマスを早熟化するか?2021

    • Author(s)
      守谷奈津子、三輪美砂子、吉崎悟朗
    • Organizer
      第114回 日本繁殖生物学会大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Germ-line Stem Cell Manipulation in Fish: Can Mackerel Produce Bluefin Tuna Gametes?2021

    • Author(s)
      Goro Yoshizaki
    • Organizer
      Woods Hole Marine Biological Laboratory
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 魚類の生殖工学:サバからマグロは産まれるか?2021

    • Author(s)
      吉崎 悟朗
    • Organizer
      千葉市科学館「大人が楽しむ科学教室」
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Germ Cell Transplantation in Fish: Mutant dnd Rainbow Trout can Produce Chinook Salmon Gametes.2021

    • Author(s)
      Goro Yoshizaki
    • Organizer
      The Society for the Study of Reproduction, Exotic Species Webinar Series
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] アジアスズキ遺伝子資源の長期保存を目指した生殖細胞移植条件の最適化2021

    • Author(s)
      市田健介, Araya Jangprai Kaosad, 矢澤良輔, 森田哲朗, 吉崎悟朗, Surintorn Boonanuntanasarn
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] "生きたクロマグロ精子を特異的に認識する モノクローナル抗体の作製"2021

    • Author(s)
      神尾茂治, 川村 亘, 矢澤良輔, 市田健介, 森田哲朗, 吉崎悟朗
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 代理親魚技法によるスマ雑種宿主からの 機能的なクロマグロ精子の生産2021

    • Author(s)
      川村亘, 神尾茂治, 矢萩はな, 谷怜央人, 矢澤良輔, 森田哲朗, 吉崎悟朗
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] マサバおよびゴマサバの性判別法の開発2021

    • Author(s)
      谷怜 央人, 矢澤 良輔, 吉崎 悟朗, Yann Guiguen
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] アユの代理親魚技術構築のための 生殖細胞移植技術の至適化2021

    • Author(s)
      天野雄一, 鈴木弘貴, 渡辺峻, 阿久津崇, 丸山隆太, 市田健介, 吉崎悟朗
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 代理親魚技術を用いたムサシトミヨ保全に向けた基礎的研究2021

    • Author(s)
      天野雄一, 山口光太郎, 吉崎悟朗
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ピンスポット飼育法を用いた slc45a2遺伝子ノックアウトマサバの作出2021

    • Author(s)
      長谷川二己, 川村亘, 山内章弘, 矢澤良輔, 森田哲朗, 吉崎悟朗
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 代理親魚技法による機能的なクロマグロ精子の生産2021

    • Author(s)
      川村 亘, 神尾 茂治, 矢澤 良輔, 吉崎 悟朗
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] slc45a2遺伝子ノックアウトによるアルビノマサバの作出2021

    • Author(s)
      長谷川 二己, 川村 亘, 山内 章弘, 矢澤 良輔, 吉崎 悟朗
    • Organizer
      令和3年度日本水産学会春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 魚類の生殖幹細胞操作:その原理と歴史2021

    • Author(s)
      吉崎悟朗
    • Organizer
      文部科学省 海洋生物資源確保技術高度化プロジェクト「生殖幹細胞操作によるクロマグロ等の新たな受精卵供給法の開発」公開シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] スマはクロマグロ配偶子生産のための適切な宿主になりうるか?2021

    • Author(s)
      矢澤良輔
    • Organizer
      文部科学省 海洋生物資源確保技術高度化プロジェクト「生殖幹細胞操作によるクロマグロ等の新たな受精卵供給法の開発」公開シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] クロマグロ生殖細胞の可視化と追跡2021

    • Author(s)
      市田健介
    • Organizer
      文部科学省 海洋生物資源確保技術高度化プロジェクト「生殖幹細胞操作によるクロマグロ等の新たな受精卵供給法の開発」公開シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] スマ宿主がクロマグロの精子を作った!2021

    • Author(s)
      川村亘
    • Organizer
      文部科学省 海洋生物資源確保技術高度化プロジェクト「生殖幹細胞操作によるクロマグロ等の新たな受精卵供給法の開発」公開シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] マスノスケだけを生み続けるニジマスの生産2021

    • Author(s)
      藤原亮
    • Organizer
      文部科学省 海洋生物資源確保技術高度化プロジェクト「生殖幹細胞操作によるクロマグロ等の新たな受精卵供給法の開発」公開シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 魚のタイムカプセル:凍結細胞から魚を作る2021

    • Author(s)
      吉崎悟朗
    • Organizer
      文部科学省 海洋生物資源確保技術高度化プロジェクト「生殖幹細胞操作によるクロマグロ等の新たな受精卵供給法の開発」公開シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 試験管内の培養細胞から魚を作る2021

    • Author(s)
      識名信也
    • Organizer
      文部科学省 海洋生物資源確保技術高度化プロジェクト「生殖幹細胞操作によるクロマグロ等の新たな受精卵供給法の開発」公開シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] 生殖幹細胞操作で拓く未来の魚類生産2021

    • Author(s)
      森田哲朗
    • Organizer
      文部科学省 海洋生物資源確保技術高度化プロジェクト「生殖幹細胞操作によるクロマグロ等の新たな受精卵供給法の開発」公開シンポジウム
    • Related Report
      2020 Annual Research Report
  • [Presentation] Development of germ cell manipulation technology in fish―Can small surrogates produce bluefin tuna gametes?2020

    • Author(s)
      Goro Yoshizaki
    • Organizer
      Cold Spring Harbor Laboratory, The 12th meeting on Germ Cells
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Book] Germline Development in the Zebrafish: Methods and Protocols2021

    • Author(s)
      Zoran Marinovic、Jelena Lujic、Qian Li、Yoshiko Iwasaki、Bela Urbanyi、Goro Yoshizaki、Akos Horvath
    • Total Pages
      383
    • Publisher
      Springer US
    • ISBN
      9781071609699
    • Related Report
      2020 Annual Research Report
  • [Book] アクアネット 2021年3月号2021

    • Author(s)
      森田哲朗、矢澤良輔、濱崎将臣、市田健介、吉崎悟朗
    • Total Pages
      4
    • Publisher
      湊文社
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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