Unraveling survival strategies of photosynthetic bacteria in extreme environments: structural and spectroscopic studies

• Project/Area Number: 20H02856
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Review Section: Basic Section 37010:Bio-related chemistry
• Research Institution: Ibaraki University
• Principal Investigator: Otomo Seiu 茨城大学, 基礎自然科学野, 教授 (10213612)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Project Status: Completed (Fiscal Year 2023)
• Budget Amount: ¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000); Fiscal Year 2023: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000); Fiscal Year 2022: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2021: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2020: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
• Keywords: 光合成 / 光捕集 / エネルギー移動 / 光電変換

Outline of Research at the Start

光合成微生物の生存にとって最も重要な器官の一つである光捕集反応中心超分子複合体(LH1-RC)は、特異な分光学的性質とユニークな立体構造をもつ。本研究では、様々な極限環境下に生きる光合成細菌（好熱、好冷、好塩、好アルカリ、好酸など）を用いて、光捕獲と光電変換を司るLH1-RC複合体の特異な分光学的挙動と、それをもたらす構造的要因を原子レベルで解明することを目的とする。本研究で得られる知見から、実用性の高い高効率の集光アンテナと光電変換素子の作成ならびに人工光合成システムの構築に対して根拠となる設計指針を与える。

Outline of Final Research Achievements

In this study, we have expanded on the previously established spectroscopic and structural studies of the light-harvesting-eaction center supramolecular complex (LH1-RC) derived from thermophilic photosynthetic bacteria, and elucidated at the atomic level aspects of the survival strategy by which photosynthetic microorganisms thriving in various extreme environments skillfully utilize solar light energy, in particular the diverse spectroscopic behaviors of the photosynthetic membranes and the light-harvesting complexes as well as the structural basis that regulates them, laying the foundation for a broader understanding of the correlation between structure and function.

Academic Significance and Societal Importance of the Research Achievements

光合成細菌から単離された光合成色素タンパク質は通常その菌体の生息環境に最も見合った安定な構造を取り、最適化された分光学的特徴を示すことから、本研究から得られた知見は様々な極限環境下に生きる光生物の光合成初期過程における励起エネルギーの伝達機構、反応中心における光誘起電荷分離機構の解明に役立つだけでなく、将来の工学的応用を見据えた人工光合成における高効率のアンテナと光電変換素子の構築に対しても根拠となる設計指針を与えるものと考えられる。

Research Products

• [Journal Article] High-resolution structure and biochemical properties of the LH1-RC photocomplex from the model purple sulfur bacterium, Allochromatium vinosum (2024)
  • Author(s): Tani Kazutoshi、Kanno Ryo、Harada Ayaka、Kobayashi Yuki、Minamino Akane、Takenaka Shinji、Nakamura Natsuki、Ji Xuan-Cheng、Purba Endang R.、Hall Malgorzata、Yu Long-Jiang、Madigan Michael T.、Mizoguchi Akira、Iwasaki Kenji、Humbel Bruno M.、Kimura Yukihiro、Wang-Otomo Zheng-Yu
  • Journal Title: Communications Biology Volume: 7 Issue: 1 Pages: 176-176
  • DOI: 10.1038/s42003-024-05863-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Selective expression of light-harvesting complexes alters phospholipid composition in the intracytoplasmic membrane and core complex of purple phototrophic bacteria (2023)
  • Author(s): Satoh I.、Gotou K.、Nagatsuma S.、Nagashima K.V.P.、Kobayashi M.、Yu L.-J.、Madigan M.T.、Kimura Y.、Wang-Otomo Z.-Y.
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1864 Issue: 4 Pages: 149001-149001
  • DOI: 10.1016/j.bbabio.2023.149001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genomic basis for the unique phenotype of the alkaliphilic purple nonsulfur bacterium Rhodobaca bogoriensis (2023)
  • Author(s): Madigan Michael T.、Bender Kelly S.、Sanguedolce Sophia A.、Parenteau Mary N.、Mayer Marisa H.、Kimura Yukihiro、Wang-Otomo Zheng-Yu、Sattley W. Matthew
  • Journal Title: Extremophiles Volume: 27 Issue: 2 Pages: 19-19
  • DOI: 10.1007/s00792-023-01304-4
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] New insights on the photocomplex of Roseiflexus castenholzii revealed from comparisons of native and carotenoid-depleted complexes (2023)
  • Author(s): Qi Chen-Hui、Wang Guang-Lei、Wang Fang-Fang、Xin Yueyong、Zou Mei-Juan、Madigan Michael T.、Wang-Otomo Zheng-Yu、Ma Fei、Yu Long-Jiang
  • Journal Title: Journal of Biological Chemistry Volume: 299 Issue: 8 Pages: 105057-105057
  • DOI: 10.1016/j.jbc.2023.105057
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Rhodobacter capsulatus forms a compact crescent-shaped LH1-RC photocomplex (2023)
  • Author(s): Tani Kazutoshi、Kanno Ryo、Ji Xuan-Cheng、Satoh Itsusei、Kobayashi Yuki、Hall Malgorzata、Yu Long-Jiang、Kimura Yukihiro、Mizoguchi Akira、Humbel Bruno M.、Madigan Michael T.、Wang-Otomo Zheng-Yu
  • Journal Title: Nature Communications Volume: 14 Issue: 1 Pages: 846-846
  • DOI: 10.1038/s41467-023-36460-w
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Advances in the Spectroscopic and Structural Characterization of Core Light-Harvesting Complexes from Purple Phototrophic Bacteria (2023)
  • Author(s): Kimura Yukihiro、Tani Kazutoshi、Madigan Michael T.、Wang-Otomo Zheng-Yu
  • Journal Title: The Journal of Physical Chemistry B Volume: 127 Issue: 1 Pages: 6-17
  • DOI: 10.1021/acs.jpcb.2c06638
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Atomic force microscopic analysis of the light-harvesting complex 2 from purple photosynthetic bacterium Thermochromatium tepidum (2023)
  • Author(s): Morimoto Masayuki、Hirao Haruna、Kondo Masaharu、Dewa Takehisa、Kimura Yukihiro、Wang-Otomo Zheng-Yu、Asakawa Hitoshi、Saga Yoshitaka
  • Journal Title: Photosynthesis Research Volume: - Issue: 1 Pages: 1-1
  • DOI: 10.1007/s11120-023-01010-4
  • Peer Reviewed
• [Journal Article] An LH1-RC photocomplex from an extremophilic phototroph provides insight into origins of two photosynthesis proteins (2022)
  • Author(s): Tani Kazutoshi、Kanno Ryo、Kurosawa Keigo、Takaichi Shinichi、Nagashima Kenji V. P.、Hall Malgorzata、Yu Long-Jiang、Kimura Yukihiro、Madigan Michael T.、Mizoguchi Akira、Humbel Bruno M.、Wang-Otomo Zheng-Yu
  • Journal Title: Communications Biology Volume: 5 Issue: 1 Pages: 1197-1197
  • DOI: 10.1038/s42003-022-04174-2
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Major Carotenoids of Meiothermus ruber Are Deinoxanthin Glucoside Esters, Not Meiothermoxanthin Glucoside Esters (2022)
  • Author(s): Asaka Ryo、Ohshima Kazuki、Kawasaki Shinji、Maoka Takashi、Tode Chisato、Wang-Otomo Zheng-Yu、Takaichi Shinichi
  • Journal Title: Journal of Natural Products Volume: 85 Issue: 10 Pages: 2266-2273
  • DOI: 10.1021/acs.jnatprod.2c00271
  • Peer Reviewed
• [Journal Article] Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1?RC complex (2022)
  • Author(s): Tani Kazutoshi、Kanno Ryo、Kikuchi Riku、Kawamura Saki、Nagashima Kenji V. P.、Hall Malgorzata、Takahashi Ai、Yu Long-Jiang、Kimura Yukihiro、Madigan Michael T.、Mizoguchi Akira、Humbel Bruno M.、Wang-Otomo Zheng-Yu
  • Journal Title: Nature Communications Volume: 13 Issue: 1 Pages: 1904-1904
  • DOI: 10.1038/s41467-022-29453-8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Carotenoid Single-Molecular Singlet Fission and the Photoprotection of a Bacteriochlorophyll b-Type Core Light-Harvesting Antenna (2022)
  • Author(s): Zhang Yan、Qi Chen-Hui、Yamano Nami、Wang Peng、Yu Long-Jiang、Wang-Otomo Zheng-Yu、Zhang Jian-Ping
  • Journal Title: The Journal of Physical Chemistry Letters Volume: 13 Issue: 16 Pages: 3534-3541
  • DOI: 10.1021/acs.jpclett.2c00519
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A Ca2+-binding motif underlies the unusual properties of certain photosynthetic bacterial core light-harvesting complexes (2022)
  • Author(s): Tani Kazutoshi、Kobayashi Kazumi、Hosogi Naoki、Ji Xuan-Cheng、Nagashima Sakiko、Nagashima Kenji V.P.、Izumida Airi、Inoue Kazuhito、Tsukatani Yusuke、Kanno Ryo、Hall Malgorzata、Yu Long-Jiang、Ishikawa Isamu、Okura Yoshihiro、Madigan Michael T.、Mizoguchi Akira、Humbel Bruno M.、Kimura Yukihiro、Wang-Otomo Zheng-Yu
  • Journal Title: Journal of Biological Chemistry Volume: - Issue: 6 Pages: 101967-101967
  • DOI: 10.1016/j.jbc.2022.101967
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Salt- and pH-dependent thermal stability of photocomplexes from extremophilic bacteriochlorophyll b-containing Halorhodospira species. (2022)
  • Author(s): Kimura, Y., Nakata, K., Nojima, S., Takenaka, S., Madigan, M. T. & Wang-Otomo, Z.-Y.
  • Journal Title: Microoganisms Volume: 10 Pages: 959-959
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Identification of metal-sensitive structural changes in the Ca²⁺-binding photocomplex from <i>Thermochromatium tepidum</i> by isotope-edited vibrational spectroscopy (2022)
  • Author(s): Kimura Yukihiro、Imanishi Michie、Li Yong、Yura Yuki、Ohno Takashi、Saga Yoshitaka、Madigan Michael T.、Wang-Otomo Zheng-Yu
  • Journal Title: The Journal of Chemical Physics Volume: 156 Issue: 10 Pages: 105101-105101
  • DOI: 10.1063/5.0075600
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Allochromatium tepidum, sp. nov., a hot spring species of purple sulfur bacteria. (2022)
  • Author(s): Madigan, M.T., Absher, J.N., Mayers, J.E., Asao, M., Jung, D.O., Bender, K.S., Kempher, M.L., Hayward, M.K., Sanguedolce, S.A., Brown, A.C., Takaichi, S., Kurokawa, K., Toyoda, A., Mori, H., Tsukatani, Y., Wang-Otomo, Z.Y., Ward, D.M., and Sattley, W.M.
  • Journal Title: Archives of Microbiology Volume: 204 Issue: 1 Pages: 115-115
  • DOI: 10.1007/s00203-021-02715-7
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Cryo-EM structure of a Ca2+-bound photosynthetic LH1-RC complex containing multiple αβ-polypeptides (2020)
  • Author(s): Tani Kazutoshi、Kanno Ryo、Makino Yuki、Hall Malgorzata、Takenouchi Mizuki、Imanishi Michie、Yu Long-Jiang、Overmann Jorg、Madigan Michael T.、Kimura Yukihiro、Mizoguchi Akira、Humbel Bruno M.、Wang-Otomo Zheng-Yu
  • Journal Title: Nature Communications Volume: 11 Issue: 1 Pages: 4955-4955
  • DOI: 10.1038/s41467-020-18748-3
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Lycopene-Family Carotenoids Confer Thermostability on Photocomplexes from a New Thermophilic Purple Bacterium (2020)
  • Author(s): Seto Ryuta、Takaichi Shinichi、Kurihara Toshiyuki、Kishi Rikako、Honda Mai、Takenaka Shinji、Tsukatani Yusuke、Madigan Michael T.、Wang-Otomo Zheng-Yu、Kimura Yukihiro
  • Journal Title: Biochemistry Volume: 59 Issue: 25 Pages: 2351-2358
  • DOI: 10.1021/acs.biochem.0c00192
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] The two light-harvesting membrane chromoproteins of Thermochromatium tepidum expose distinct robustness against temperature and pressure (2020)
  • Author(s): Kangur Liina、Ratsep Margus、Timpmann K.、Wang-Otomo Zheng-Yu、Freiberg Arvi
  • Journal Title: Biochimica et Biophysica Acta (BBA) - Bioenergetics Volume: 1861 Issue: 8 Pages: 148205-148205
  • DOI: 10.1016/j.bbabio.2020.148205
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Direct injection of pigment-protein complexes and membrane fragments suspended in water from phototrophs to C18 HPLC (2020)
  • Author(s): Takaichi Shinichi、Okoshi Akira、Otomo Seiu、Misumi Masahiro、Sonoike Kintake、Harada Jiro
  • Journal Title: Photosynthesis Research Volume: 144 Issue: 1 Pages: 101-107
  • DOI: 10.1007/s11120-020-00735-w
  • Peer Reviewed
• [Presentation] 昨年発表した「紅色細菌の昇温培養に対するカロテノイド生合成系の調節」のその後 (2022)
  • Author(s): 大友征宇
  • Organizer: 光合成セミナー2022
• [Presentation] 極限環境下に生息する紅色細菌の光捕集複合体の多様性 (2020)
  • Author(s): 大友征宇
  • Organizer: 第５回光合成細菌ワークショップ
  • Invited
• [Book] 光合成細菌 (2020)
  • Author(s): 浅井智広、井上和仁、大友征宇、嶋田敬三、高市真一、塚谷祐介、永島賢治、原田二朗、平石明
  • Total Pages: 320
  • Publisher: 裳華房
  • ISBN: 9784785358709