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Uncovering the molecular basis of nitrogen starvation responses

Research Project

Project/Area Number 20H02888
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 38010:Plant nutrition and soil science-related
Research InstitutionNagoya University

Principal Investigator

Kiba Takatoshi  名古屋大学, 生命農学研究科, 准教授 (20532097)

Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2023: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2022: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2021: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2020: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Keywords窒素飢餓 / 窒素欠乏 / 窒素栄養 / 植物 / 情報伝達 / 分子メカニズム / 窒素欠乏応答 / 窒素飢餓応答
Outline of Research at the Start

植物が自然環境でしばしば遭遇する比較的長期間に渡る窒素不足状態「窒素飢餓」をどのように感知・応答するのかについて、その分子メカニズムの理解は進んでいない。本申請研究では、シロイヌナズナにおいて申請者独自の窒素飢餓マーカーを用いた徹底的な新奇因子探索、新奇・既知因子の役割分担と因子間の遺伝的関係の明確化を通して、植物における窒素飢餓感知から出力に至る情報伝達の分子基盤を体系的に理解することを目標とする。また窒素飢餓応答制御メカニズムのイネとエノコログサにおける普遍性と多様性について調べ、窒素飢餓耐性や窒素低要求性作物など、低投入型持続的農業に適した優良品種の開発につながる知見を得ることも目指す。

Outline of Final Research Achievements

In this study, we aimed to understand the molecular basis of the nitrogen starvation response regulation in plants, from perception to output, by exploring novel factors using nitrogen starvation markers in Arabidopsis and reevaluating known factors involved in nitrogen response regulation. Through mutant screening and yeast one-hybrid screening, we successfully identified multiple novel candidate regulators. Additionally, reevaluation of the known factors revealed that the NLP and LBD transcription factor families play a more significant role in controlling nitrogen starvation response than previously thought.

Academic Significance and Societal Importance of the Research Achievements

本研究によって、植物の窒素飢餓応答制御機構の理解が大きく進んだ。近年窒素肥料の過剰な使用が、コスト高を招いているだけでなく深刻な環境問題をも引き起こしており、世界的に低投入持続型農業への移行が求められている。窒素飢餓応答機構の解明は、窒素飢餓耐性植物や窒素低要求性植物など、低投入型持続的農業に適した新たな優良品種の開発につながるため、社会的インパクトも大きい。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (30 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (4 results) Journal Article (15 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 15 results,  Open Access: 13 results) Presentation (8 results) (of which Invited: 1 results) Remarks (3 results)

  • [Int'l Joint Research] Technical University of Madrid(スペイン)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] University of Jena/Dresden University of Technology(ドイツ)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] INRA-AgroParisTech/CNRS(フランス)

    • Related Report
      2023 Annual Research Report
  • [Int'l Joint Research] Donald Danforth Plant Science Center,/HudsonAlpha Institute for Biotechnology,(米国)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] The trans-zeatin-type side-chain modification of cytokinins controls rice growth2023

    • Author(s)
      Kiba Takatoshi、Mizutani Kahori、Nakahara Aimi、Takebayashi Yumiko、Kojima Mikiko、Hobo Tokunori、Osakabe Yuriko、Osakabe Keishi、Sakakibara Hitoshi
    • Journal Title

      Plant Physiology

      Volume: 192 Issue: 3 Pages: 2457-2474

    • DOI

      10.1093/plphys/kiad197

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Root-Colonizing Endophyte Piriformospora indica Supports Nitrogen-Starved Arabidopsis thaliana Seedlings with Nitrogen Metabolites2023

    • Author(s)
      Scholz Sandra S.、Barth Emanuel、Clement Gilles、Marmagne Anne、Ludwig-Muller Jutta、Sakakibara Hitoshi、Kiba Takatoshi、Vicente-Carbajosa Jesus、Pollmann Stephan、Krapp Anne、Oelmuller Ralf
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 24 Issue: 20 Pages: 15372-15372

    • DOI

      10.3390/ijms242015372

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 植物の低窒素環境における生存戦略2022

    • Author(s)
      大久保祐里, 松林嘉克, 木羽 隆敏
    • Journal Title

      KAGAKU TO SEIBUTSU

      Volume: 60 Issue: 7 Pages: 352-360

    • DOI

      10.1271/kagakutoseibutsu.60.352

    • ISSN
      0453-073X, 1883-6852
    • Year and Date
      2022-07-01
    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of gametes and zygotes from Setaria viridis2022

    • Author(s)
      Toda Erika、Kiba Takatoshi、Kato Norio、Okamoto Takashi
    • Journal Title

      Journal of Plant Research

      Volume: 135 Issue: 4 Pages: 627-633

    • DOI

      10.1007/s10265-022-01393-w

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] ABCG11 modulates cytokinin responses in Arabidopsis thaliana2022

    • Author(s)
      Yang Qianying、Zhang Jie、Kojima Mikiko、Takebayashi Yumiko、Uragami Takuya、Kiba Takatoshi、Sakakibara Hitoshi、Lee Youngsook
    • Journal Title

      Frontiers in Plant Science

      Volume: 13 Pages: 1-12

    • DOI

      10.3389/fpls.2022.976267

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The calcium sensor CBL7 is required for Serendipita indica-induced growth stimulation in Arabidopsis thaliana, controlling defense against the endophyte and K+ homoeostasis in the symbiosis.2022

    • Author(s)
      Perez-Alonso MM, Guerrero-Galan C, Gonzalez Ortega-Villaizan A, Ortiz-Garcia P, Scholz SS, Ramos P, Sakakibara H, Kiba T, Ludwig-Muller J, Krapp A, Oelmuller R, Vicente-Carbajosa J, Pollmann S.
    • Journal Title

      Plant Cell Environ.

      Volume: 45 Issue: 11 Pages: 3367-3382

    • DOI

      10.1111/pce.14420

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Photosynthetic-Product?Dependent Activation of Plasma Membrane H+-ATPase and Nitrate Uptake in<i>Arabidopsis</i>Leaves2022

    • Author(s)
      Kinoshita Satoru N、Suzuki Takamasa、Kiba Takatoshi、Sakakibara Hitoshi、Kinoshita Toshinori
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 2 Pages: 191-203

    • DOI

      10.1093/pcp/pcac157

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 2. Mechanism of nitrogen transport in plants2021

    • Author(s)
      木羽 隆敏, 小西 美稲子, 柳澤 修一
    • Journal Title

      Japanese Journal of Soil Science and Plant Nutrition

      Volume: 92 Issue: 2 Pages: 76-91

    • DOI

      10.20710/dojo.92.2_76

    • NAID

      130008024162

    • ISSN
      0029-0610, 2424-0583
    • Year and Date
      2021-04-05
    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana2021

    • Author(s)
      Hachiya Takushi、Inaba Jun、Wakazaki Mayumi、Sato Mayuko、Toyooka Kiminori、Miyagi Atsuko、Kawai-Yamada Maki、Sugiura Daisuke、Nakagawa Tsuyoshi、Kiba Takatoshi、Gojon Alain、Sakakibara Hitoshi
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 4944-4944

    • DOI

      10.1038/s41467-021-25238-7

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Low nitrogen conditions accelerate flowering by modulating the phosphorylation state of FLOWERING BHLH 4 in <i>Arabidopsis</i>2021

    • Author(s)
      Sanagi Miho、Aoyama Shoki、Kubo Akio、Lu Yu、Sato Yasutake、Ito Shogo、Abe Mitsutomo、Mitsuda Nobutaka、Ohme-Takagi Masaru、Kiba Takatoshi、Nakagami Hirofumi、Rolland Filip、Yamaguchi Junji、Imaizumi Takato、Sato Takeo
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 118 Issue: 19 Pages: 1-12

    • DOI

      10.1073/pnas.2022942118

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NIGT1 family proteins exhibit dual mode DNA recognition to regulate nutrient responseassociated genes in Arabidopsis2020

    • Author(s)
      Ueda Yoshiaki、Nosaki Shohei、Sakuraba Yasuhito、Miyakawa Takuya、Kiba Takatoshi、Tanokura Masaru、Yanagisawa Shuichi
    • Journal Title

      PLOS Genetics

      Volume: 16 Issue: 11 Pages: 1-27

    • DOI

      10.1371/journal.pgen.1009197

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A genome resource for green millet Setaria viridis enables discovery of agronomically valuable loci2020

    • Author(s)
      Mamidi Sujan、Healey Adam、Huang Pu、Grimwood Jane、Jenkins Jerry、Barry Kerrie、Sreedasyam Avinash、Shu Shengqiang、Lovell John T.、Feldman Maximilian、Wu Jinxia、Yu Yunqing、Chen Cindy、Johnson Jenifer、Sakakibara Hitoshi、Kiba Takatoshi et al.
    • Journal Title

      Nature Biotechnology

      Volume: 38 Issue: 10 Pages: 1203-1210

    • DOI

      10.1038/s41587-020-0681-2

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Nitrate‐inducible NIGT1 proteins modulate phosphate uptake and starvation signalling via transcriptional regulation of SPX genes2020

    • Author(s)
      Ueda Yoshiaki、Kiba Takatoshi、Yanagisawa Shuichi
    • Journal Title

      The Plant Journal

      Volume: 102 Issue: 3 Pages: 448-466

    • DOI

      10.1111/tpj.14637

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nitrogen and Phosphorus interactions in plants: from agronomic to physiological and molecular insights.2020

    • Author(s)
      Takatoshi Kiba、Gabriel Krouk
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 57 Pages: 104-109

    • DOI

      10.1016/j.pbi.2020.07.002

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Harnessing symbiotic plant-fungus interactions to unleash hidden forces from extreme plant ecosystems2020

    • Author(s)
      Perez-Alonso M.M., Guerrero-Galan, C., Scholz, S.S., Kiba, T., Sakakibara, H., Ludwig-Muller, J., Krapp, A., Oelmuller, R., Vicente-Carbajosa, J., Pollmann, S.
    • Journal Title

      Journal of Experimental Botany

      Volume: 54 Issue: 13 Pages: 137-142

    • DOI

      10.1093/jxb/eraa040

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] ABCG14を介した根から地上部へのサイトカイニン長距離輸送のメカニズム2023

    • Author(s)
      水谷 佳保里、竹林 裕美子、小嶋 美紀子、 榊原 均、木羽 隆敏
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] 窒素欠乏応答制御におけるAtPHLsの役割2023

    • Author(s)
      柏田星南、木羽隆敏
    • Organizer
      日本植物学会第87回大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] The trans-hydroxylation of cytokinin by CYP735A3 and CYP735A4 controls growth and development in rice2023

    • Author(s)
      Takatoshi Kiba, Kahori Mizutani, Aimi Nakahara, Yumiko Takebayashi, Mikiko Kojima, Tokunori Hobo, Yuriko Osakabe, Keishi Osakabe, and Hitoshi Sakakibara
    • Organizer
      第64回日本植物生理学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 窒素欠乏応答制御における NIGT1s と LBDs の役割2022

    • Author(s)
      高橋 花, Fanny Bellegarde, 木羽 隆敏
    • Organizer
      植物の栄養研究会第7回交流会
    • Related Report
      2022 Annual Research Report
  • [Presentation] The physiological role of trans-zeatin-type cytokinins in rice2022

    • Author(s)
      Takatoshi Kiba, Kahori Mizutani, Yumiko Takebayashi, Mikiko Kojima, Tokunori Hobo, Hitoshi Sakakibara
    • Organizer
      第63回日本植物生理学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 植物ホルモン・サイトカイニンによる植物成長制御 長距離輸送と側鎖修飾の仕組みと役割2021

    • Author(s)
      木羽隆敏
    • Organizer
      第378回細胞工学研究会講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Identification of cis-regulatory elements that confers high expression2021

    • Author(s)
      木羽隆敏、工藤徹、矢野健太郎、加藤紀夫
    • Organizer
      第62回植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ABCG14を介した根から地上部へのサイトカイニン長距離輸送のメカニズム2020

    • Author(s)
      木羽隆敏、橋本祐典、小嶋美紀子、竹林裕美子、榊原均
    • Organizer
      日本植物学会第84回大会
    • Related Report
      2020 Annual Research Report
  • [Remarks] https://www.agr.nagoya-u.ac.jp/~ck/

    • Related Report
      2023 Annual Research Report
  • [Remarks] https://www.agr.nagoya-u.ac.jp/~ck/

    • Related Report
      2022 Annual Research Report
  • [Remarks] https://www.agr.nagoya-u.ac.jp/~ck/

    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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