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Elucidation of mechanism of ''re-greening'' phenomenon in citrus fruit

Research Project

Project/Area Number 20H02976
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionShizuoka University

Principal Investigator

Kato Masaya  静岡大学, 農学部, 教授 (10432197)

Co-Investigator(Kenkyū-buntansha) 張 嵐翠  静岡大学, 農学部, 特任助教 (20767371)
馬 剛  静岡大学, 農学部, 助教 (20767412)
本橋 令子  静岡大学, 農学部, 教授 (90332296)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2023: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2022: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2021: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2020: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Keywordsカンキツ / 回青 / カロテノイド / クロロフィル / フラベド / フラボノイド
Outline of Research at the Start

「回青(かいせい)」とはカンキツ特有の現象であり、樹上で一旦成熟した橙色の果実が緑色の果実へと戻る現象である。本研究課題では、「回青」の認められるカンキツ果実における二次代謝産物および果実品質に関わる糖、有機酸、栄養成分の代謝変動を調査し、「回青」現象の発生や果実成熟を調節する遺伝子を同定する。以上のような研究を行うことにより、カンキツ果実における「回青」現象の発生メカニズムを解明し、カンキツ果実の成熟を制御する栽培および収穫後の技術の開発に繋げる。

Outline of Final Research Achievements

In this study, to elucidate the regulatory mechanism of regreening phenomenon in citrus fruit, light irradiation and plant hormones were treated on the fruits on the tree and the cultured flavedos. The results showed that blue light irradiation and Gibberellic acid (GA) treatments promoted the chlorophyll accumulation by increasing the expression level of chlorophyll biosynthetic genes and decreasing the expression level of chlorophyll degradation genes. Whereas, red light, abscisic acid (ABA) and low nitrogen treatments inhibited the chlorophyll accumulation by decreasing the expression levels of chlorophyll biosynthetic genes and increasing the expression levels of chlorophyll degradation genes. These results indicate that the regreening of citrus fruit is positively regulated by blue light and GA, while negatively regulated by red light, ABA and low nitrogen.

Academic Significance and Societal Importance of the Research Achievements

本研究で明らかにしたカンキツ果実特有の「回青」現象の発生メカニズムはカンキツ果実の成熟過程における着色機構の全容の解明に寄与し、果実着色に関わる光照射、植物ホルモンおよび栄養元素の役割の解明に貢献する。また、回青の発生の鍵転写因子候補の特定はDNAマーカーを利用した回青が発生しにくい新品種の育成に繋がる。さらに、果実の着色技術を開発することで、カンキツ果実の市場価値の向上やロスの減少に直結する。

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Annual Research Report
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (31 results)

All 2024 2023 2022 2021 2020

All Journal Article (9 results) (of which Peer Reviewed: 9 results,  Open Access: 3 results) Presentation (21 results) (of which Int'l Joint Research: 6 results) Book (1 results)

  • [Journal Article] Characterization of granulation in citrus “Harumi” fruit during postharvest storage2024

    • Author(s)
      Deng Zhiwei、Ma Gang、Zhang Lancui、Kurata Daiki、Ikeya Masato、Keawmanee Nichapat、Nonaka Keisuke、Takishita Fumitaka、Kato Masaya
    • Journal Title

      Postharvest Biology and Technology

      Volume: 210 Pages: 112770-112770

    • DOI

      10.1016/j.postharvbio.2024.112770

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of Chlorophyll and Carotenoid Metabolism in Citrus Fruit During Maturation and Regreening2023

    • Author(s)
      Keawmanee Nichapat、Ma Gang、Zhang Lancui、Kato Masaya
    • Journal Title

      Reviews in Agricultural Science

      Volume: 11 Issue: 0 Pages: 203-216

    • DOI

      10.7831/ras.11.0_203

    • ISSN
      2187-090X
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Postharvest Treatment with 1-Naphthaleneacetic Acid on Chlorophyll and Carotenoid Metabolism in Citrus Fruit2023

    • Author(s)
      Ma Gang、Zhang Lancui、Murakami Kan、Yahata Masaki、Kato Masaya
    • Journal Title

      The Horticulture Journal

      Volume: 92 Issue: 4 Pages: 393-401

    • DOI

      10.2503/hortj.QH-082

    • ISSN
      2189-0102, 2189-0110
    • Related Report
      2023 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular regulation of carotenoid biosynthesis in citrus juice sacs: New advances2023

    • Author(s)
      Gang Ma, Lancui Zhang, Masaya Kato
    • Journal Title

      Sciential Horticulturae

      Volume: 309 Pages: 111629-111629

    • DOI

      10.1016/j.scienta.2022.111629

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of blue LED light irradiation on chlorophyll metabolism in the flavedo of Valencia orange2022

    • Author(s)
      Mochizuki H.、Kitaya Y.、Ma G.、Zhang L.C.、Yahata M.、Kato M.
    • Journal Title

      Acta Horticulturae

      Volume: 1336 Issue: 1336 Pages: 117-124

    • DOI

      10.17660/actahortic.2022.1336.16

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Microarray analysis of carotenoid and chlorophyll biosynthetic gene expression in the citrus juice sacs irradiated with blue light2022

    • Author(s)
      Gang Ma, Lancui Zhang, Masaki Yahata, Kazuki Yamawaki, Takehiko Shimada, Hiroshi Fujii, Tomoko Endo, Masaya Kato.
    • Journal Title

      Acta Horticulturae

      Volume: 1337 Issue: 1337 Pages: 195-202

    • DOI

      10.17660/actahortic.2022.1337.26

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Exogenous gibberellin induced regreening through the regulation of chlorophyll and carotenoid metabolism in Valencia oranges2022

    • Author(s)
      Nichapat Keawmanee, Gang Ma, Lancui Zhang, Masaki Yahata, Kan Murakami, Masashi Yamamoto, Nami Kojima, Masaya Kato
    • Journal Title

      Plant Physiology and Biochemistry

      Volume: 173 Pages: 14-24

    • DOI

      10.1016/j.plaphy.2022.01.021

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Blue LED light induces regreening in the flavedo of Valencia orange in vitro2021

    • Author(s)
      Ma Gang、Zhang Lancui、Kitaya Yurika、Seoka Mao、Kudaka Rin、Yahata Masaki、Yamawaki Kazuki、Shimada Takehiko、Fujii Hiroshi、Endo Tomoko、Kato Masaya
    • Journal Title

      Food Chemistry

      Volume: 335 Pages: 127621-127621

    • DOI

      10.1016/j.foodchem.2020.127621

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effects of salicylic acid and methyl jasmonate treatments on flavonoid and carotenoid accumulation in the juice sacs of Satsuma Mandarin in vitro2020

    • Author(s)
      Risa Yamamoto*, Gang Ma*, Lancui Zhang, Miki Hirai, Masaki Yahata, Kazuki Yamawaki, Takehiko Shimada, Hiroshi Fujii, Tomoko Endo, Masaya Kato (*These authors contributed equally)
    • Journal Title

      Applied Science

      Volume: 10 Issue: 24 Pages: 8916-8916

    • DOI

      10.3390/app10248916

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 貯蔵中のウンシュウミカン果実におけるカロテノイドおよびクロロフィル代謝に及ぼすジャスモン酸メチルおよびサリチル酸の樹上散布処理の影響2024

    • Author(s)
      池谷将斗、馬剛、張嵐翠、八幡昌紀、加藤雅也
    • Organizer
      園芸学会令和6年度春季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] カンキツ果実におけるフラボノイド生合成に関わるカルコンシンターゼ遺伝子の発現および機能解析2024

    • Author(s)
      光川早紀、馬剛、張嵐翠、八幡昌紀、加藤雅也
    • Organizer
      園芸学会令和6年度春季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] カンキツ果実におけるフラボノイド代謝分解に関わるβ-グルコシダーゼの酵素学的性質および遺伝子の発現解析2024

    • Author(s)
      中川佳音、馬剛 、張嵐翠、八幡昌紀、島田武彦、藤井浩、遠藤朋子、加藤雅也
    • Organizer
      園芸学会令和6年度春季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Effects of polyethylene wrapping on carotenoid accumulation in granulated ‘Harumi’ fruit during storage2024

    • Author(s)
      鄧志偉、馬剛、張嵐翠、倉田大輝、野中圭介、瀧下文孝、加藤雅也
    • Organizer
      園芸学会令和6年度春季大会
    • Related Report
      2023 Annual Research Report
  • [Presentation] Effect of fruit bagging on chlorophyll and carotenoid accumulation in flavedo of Valencia orange during regreening2023

    • Author(s)
      Nichapat Keawmanee、Gang Ma、Lancui Zhang、Masaki Yahata、Fumitaka Takishita、Masaya Kato
    • Organizer
      The 4th Asian Horticultural Congress (AHC2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effect of methyl jasmonate on the coloration of Satsuma mandarin fruit2023

    • Author(s)
      Masato Ikeya、Gang Ma、Lancui Zhang、Masaki Yahata、Masaya Kato
    • Organizer
      The 4th Asian Horticultural Congress (AHC2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Accumulation of 9-cis-Violaxanthin esters and expression of xanthophyll esterase genes in Valencia orange during the regreening process2023

    • Author(s)
      Mika Kato、Gang Ma、Lancui Zhang、Masaki Yahata、Masaya Kato
    • Organizer
      The 4th Asian Horticultural Congress (AHC2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Accumulation of flavonoid and expression of flavonoid biosynthetic genes in citrus fruit during maturation2023

    • Author(s)
      Saki Mitsukawa、Gang Ma、Lancui Zhang、Masaki Yahata、Masaya Kato
    • Organizer
      The 4th Asian Horticultural Congress (AHC2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Isolation and expression analysis of two novel chalcone synthase genes in different varieties of citrus fruit during maturation2023

    • Author(s)
      Chisato Torimoto、Gang Ma、Lancui Zhang、Masaki Yahata、Masaya Kato
    • Organizer
      The 4th Asian Horticultural Congress (AHC2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Changes in carotenoid accumulation and the expression of carotenoid metabolic genes during the granulation process in different sizes of ‘Harumi’ fruit2023

    • Author(s)
      Zhiwei Deng、Gang Ma、Lancui Zhang、Daiki Kurata、Keisuke Nonaka、Fumitaka Takishita、Masaya Kato
    • Organizer
      The 4th Asian Horticultural Congress (AHC2023)
    • Related Report
      2023 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 培養したバレンシアオレンジのフラベドにおけるカロテノイドおよびクロロフィル代謝に及ぼすアブシシン酸受容体阻害剤の影響2023

    • Author(s)
      望月春奈、馬 剛、張 嵐翠、八幡昌紀、轟 泰司、竹内 純、加藤雅也
    • Organizer
      園芸学会令和5年度春季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 植物ホルモンを処理したバレンシアオレンジの培養したフラベドにおけるマイクロアレイ解析2022

    • Author(s)
      望月春奈、馬 剛、張 嵐翠、八幡昌紀、島田武彦、藤井 浩、遠藤朋子、加藤雅也
    • Organizer
      芸学会令和4年度秋季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Effect of blue LED light irradiation on chlorophyll metabolism in the flavedo of Valencia orange in vitro2022

    • Author(s)
      Haruna Mochizuki, Yurika Kitaya, Gang Ma, Lancui Zhang, Masaki Yahata and Masaya Kato
    • Organizer
      V Asia Symposium on Quality Management in Postharvest Systems (ISHS)
    • Related Report
      2022 Annual Research Report
  • [Presentation] Characterization of Pigments Accumulation in Valencia Orange During Regreening2022

    • Author(s)
      Nichapat Keawmanee, Gang Ma, Lancui Zhang, Masaki Yahata, Masaya Kato
    • Organizer
      V Asia Symposium on Quality Management in Postharvest Systems (ISHS)
    • Related Report
      2022 Annual Research Report
  • [Presentation] Effect of fruit bagging with different-color bags on carotenoid accumulation in flavedo of Valencia orange2022

    • Author(s)
      Keawmanee Nichapat, 馬 剛, 張嵐翠, 八幡昌紀, 瀧下文孝, 加藤雅也
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 培養した‘太田ポンカン’のフラベドにおけるカロテノイド,クロロフィルおよびジベレリン代謝に及ぼすジベレリンの影響2022

    • Author(s)
      望月春奈, 馬 剛, 張嵐翠, 八幡昌紀, 加藤雅也
    • Organizer
      園芸学会令和4年度春季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Expression analysis of genes involved in carotenoid and chlorophyll biosyntheses in Valencia orange fruit during regreening2021

    • Author(s)
      Keawmanee Nichapat,馬剛,張嵐翠,八幡昌紀,加藤雅也
    • Organizer
      園芸学会令和3年度秋季大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] ‘太田ポンカン’のフラベドにおけるカロテノイドおよびクロロフィル代謝に及ぼす植物ホルモンの影響2021

    • Author(s)
      北谷友梨佳,望月春奈,馬 剛,張 嵐翠,八幡昌紀,山脇和樹,加藤雅也
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] バレンシアオレンジのフラベドにおけるカロテノイド及びクロロフィル代謝に及ぼす植物ホルモンの影響2021

    • Author(s)
      望月春奈,北谷友梨佳,馬 剛,張 嵐翠,八幡昌紀,山脇和樹,加藤雅也
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Carotenoid and chlorophyll accumulation in Valencia orange during regreening2021

    • Author(s)
      Keawmanee Nichapat,馬 剛,張嵐翠,八幡昌紀,山脇和樹,加藤雅也
    • Organizer
      園芸学会令和3年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ‘太田ポンカン’のフラベドにおけるカロテノイドおよびクロロフィル代謝に及ぼす光照射の影響2020

    • Author(s)
      北谷友梨佳,馬 剛,張 嵐翠,八幡昌紀,山脇和樹,加藤雅也
    • Organizer
      園芸学会令和2年度春季大会
    • Related Report
      2020 Annual Research Report
  • [Book] カロテノイドの科学(第17章カンキツ果実におけるβ-クリプトキサンチンの生合成機構およびヒトへの 機能性 分担)2024

    • Author(s)
      加藤 雅也、馬 剛、張 嵐翠
    • Total Pages
      275
    • Publisher
      シーエムシー出版
    • ISBN
      9784781317984
    • Related Report
      2023 Annual Research Report

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Published: 2020-04-28   Modified: 2025-01-30  

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