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Impacts of lignin structural variations on the functions and properties of lignocellulose as studied with lignin-modified engineered plants

Research Project

Project/Area Number 20H03044
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 40020:Wood science-related
Research InstitutionKyoto University

Principal Investigator

Tobimatsu Yuki  京都大学, 生存圏研究所, 教授 (20734221)

Co-Investigator(Kenkyū-buntansha) 堀 千明  北海道大学, 工学研究院, 助教 (50722948)
久住 亮介  京都大学, 農学研究科, 助教 (70546530)
梅澤 俊明  京都大学, 生存圏研究所, 教授 (80151926)
今井 友也  京都大学, 生存圏研究所, 教授 (90509142)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2022: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2021: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2020: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
KeywordsLignin / lignocellulose / cell wall / bioengineering / supramolecular structure / NMR / X-ray / genome editting / リグニン / フェルラ酸 / 細胞壁 / リグノセルロース / セルロース / ヘミセルロース
Outline of Research at the Start

複雑多様なリグニンの分子構造は、維管束植物の進化・環境適応と密接に関係する木質細胞壁の本質的特徴であると同時に、脱炭素社会構築を担う木質利用の重要阻害要因である。本研究は、木質の生理機能・超分子構造・利用特性に及ぼすリグニンの寄与を体系的に明らかにすることを目的とする。リグニンの量・構造を系統的に変化させたリグニン改変組換え植物を作出し、その細胞壁の化学構造・超分子構造・生理学的特性・化学変換利用特性を体系的に調べ、改変されたリグニンの量・構造との関係性を明らかにする。同時に、改変したリグニンの反応特性に合わせた木質の成分分離・触媒分解反応の最適化を通じ、木質変換利用効率の相乗的向上を図る。

Outline of Final Research Achievements

The structural diversity of lignin in secondary cell walls is closely related to the evolution and environmental adaptation of vascular plants, whereas it also acts as a key inhibitory factor for the utilization of lignocellulosic biomass. The objective of this study was to elucidate the biosynthetic mechanism of lignin monomers that contribute to the formation of diverse lignin substructures. Additionally, we aimed to establish the relationship between the molecular structure of lignin and the functions and properties of lignocellulose. To achieve these goals, we identified several enzyme genes involved in the biosynthesis of the diverse lignin monomers in rice and engineered the identified genes to generate new lignin-modified rice mutant and transgenic lines. Subsequently, we examined the supramolecular structure and biomass properties of lignocellulose from the developed rice mutants and transgenic lines to investigate the impacts of the altered amount and structure of lignin.

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、維管束植物の進化や環境適応の仕組みの理解に繋がる細胞壁の機能発現におけるリグニンの役割の理解に重要な基盤を与えるものである。同時に、循環型社会構築に向けた木質バイオマス利用におけるリグニンによる阻害機構の理解や代謝工学や分子育種を通じた利用性を向上させたバイオマス生産植物の開発に寄与する知見が得られた。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (69 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (14 results) Journal Article (18 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 18 results,  Open Access: 10 results) Presentation (36 results) (of which Int'l Joint Research: 11 results,  Invited: 7 results) Remarks (1 results)

  • [Int'l Joint Research] ウィスコンシン大学/ワシントン州立大学/ブルックヘブン国立研究所(米国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] 香港大学(中国)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] 高等科学研究院(CSIC)(スペイン)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] 国立農業・食料・環境研究所(INRAE)(フランス)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] 国立研究革新庁(BRIN)(インドネシア)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] ウィスコンシン大学/ワシントン州立大学/ブルックヘブン国立研究所(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 香港大学(中国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 高等科学研究院(CSIC)(スペイン)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 国立研究革新庁(BRIN)(インドネシア)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] 国立農業・食料・環境研究所(INRAE)(フランス)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] ウィスコンシン大学/オクラホマ大学/ワシントン州立大学(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 高麗大学校(韓国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] フランス国立農学研究所INRA/トーゥルーズ生物工学研究所TBI(フランス)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] ワーゲニンゲン大学(オランダ)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Effects of dietary variation on lignocellulose degradation and physiological properties of Nicobium hirtum larvae2023

    • Author(s)
      Krishanti Ni Putu Ratna Ayu、Tobimatsu Yuki、Afifi Osama Ahmed、Tarmadi Didi、Himmi Setiawan Khoirul、Umezawa Toshiaki、Ohmura Wakako、Yoshimura Tsuyoshi
    • Journal Title

      Journal of Wood Science

      Volume: 69 Issue: 1 Pages: 2-2

    • DOI

      10.1186/s10086-022-02074-x

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Existence of Syringyl α-Carbonyl-Type Tetrahydrofuran β-β Structure in Hardwood Lignins2022

    • Author(s)
      Kishimoto Takao、Hiyama Ayumi、Yamashita Ayana、Takano Toshiyuki、Tobimatsu Yuki、Urabe Daisuke
    • Journal Title

      ACS Sustainable Chemistry & Engineering

      Volume: 10 Issue: 37 Pages: 12394-12401

    • DOI

      10.1021/acssuschemeng.2c03861

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Genome-edited rice deficient in two <i>4-COUMARATE:COENZYME A LIGASE</i> genes displays diverse lignin alterations2022

    • Author(s)
      Afifi Osama Ahmed、Tobimatsu Yuki、Lam Pui Ying、Martin Andri Fadillah、Miyamoto Takuji、Osakabe Yuriko、Osakabe Keishi、Umezawa Toshiaki
    • Journal Title

      Plant Physiology

      Volume: 190 Issue: 4 Pages: 2155-2172

    • DOI

      10.1093/plphys/kiac450

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pathogen-induced autophagy regulates monolignol transport and lignin formation in plant immunity2022

    • Author(s)
      Jeon Hwi Seong、Jang Eunjeong、Kim Jinwoo、Kim Seu Ha、Lee Myoung-Hoon、Nam Myung Hee、Tobimatsu Yuki、Park Ohkmae K.
    • Journal Title

      Autophagy

      Volume: 19 Issue: 2 Pages: 597-615

    • DOI

      10.1080/15548627.2022.2085496

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lignocellulose molecular assembly and deconstruction properties of lignin-altered rice mutants2022

    • Author(s)
      Martin Andri Fadillah、Tobimatsu Yuki、Lam Pui Ying、Matsumoto Naoyuki、Tanaka Takuto、Suzuki Shiro、Kusumi Ryosuke、Miyamoto Takuji、Takeda-Kimura Yuri、Yamamura Masaomi、Koshiba Taichi、Osakabe Keishi、Osakabe Yuriko、Sakamoto Masahiro、Umezawa Toshiaki
    • Journal Title

      Plant Physiology

      Volume: 191 Issue: 1 Pages: 70-86

    • DOI

      10.1093/plphys/kiac432

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] CNSL(カシューナッツ殻油)給与の乳牛へ及ぼす効果2022

    • Author(s)
      濵嶋洋輔、宮島吉範、鈴木保宜、北澤春樹、飛松裕基、梅澤俊明、田森航也、犬童優樹、疋田千枝、冷牟田修一
    • Journal Title

      畜産技術

      Volume: 805 Pages: 21-26

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural basis of lignocellulose deconstruction by the wood-feeding anobiid beetle Nicobium hirtum2022

    • Author(s)
      Krishanti Ni Putu Ratna Ayu、Tobimatsu Yuki、Miyamoto Takuji、Fujimoto Izumi、Kartika Titik、Umezawa Toshiaki、Hata Toshimitsu、Yoshimura Tsuyoshi
    • Journal Title

      Journal of Wood Science

      Volume: 68 Issue: 1 Pages: 10-10

    • DOI

      10.1186/s10086-022-02017-6

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Tricin Biosynthesis and Bioengineering2021

    • Author(s)
      Lam Pui Ying、Lui Andy C. W.、Wang Lanxiang、Liu Hongjia、Umezawa Toshiaki、Tobimatsu Yuki、Lo Clive
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 733198-733198

    • DOI

      10.3389/fpls.2021.733198

    • NAID

      120007189280

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Monolignol acyltransferase for lignin p-hydroxybenzoylation in Populus2021

    • Author(s)
      Zhao Yunjun、Yu Xiaohong、Lam Pui-Ying、Zhang Kewei、Tobimatsu Yuki、Liu Chang-Jun
    • Journal Title

      Nature Plants

      Volume: 7 Issue: 9 Pages: 1288-1300

    • DOI

      10.1038/s41477-021-00975-1

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] New Insights on Structures Forming the Lignin-Like Fractions of Ancestral Plants2021

    • Author(s)
      Rencoret Jorge、Gutierrez Ana、Marques Gisela、del Rio Jose C.、Tobimatsu Yuki、Lam Pui Ying、Perez-Boada Marta、Ruiz-Duenas Francisco Javier、Barrasa Jose M.、Martinez Angel T.
    • Journal Title

      Frontiers in Plant Science

      Volume: 12 Pages: 740923-740923

    • DOI

      10.3389/fpls.2021.740923

    • NAID

      120007189279

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Deficiency in flavonoid biosynthesis genes CHS, CHI, and CHIL alters rice flavonoid and lignin profiles2021

    • Author(s)
      Lam Pui Ying、Wang Lanxiang、Lui Andy C W、Liu Hongjia、Takeda-Kimura Yuri、Chen Mo-Xian、Zhu Fu-Yuan、Zhang Jianhua、Umezawa Toshiaki、Tobimatsu Yuki、Lo Clive
    • Journal Title

      Plant Physiology

      Volume: 188 Issue: 4 Pages: 1993-2011

    • DOI

      10.1093/plphys/kiab606

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Termite gut microbiota contribution to wheat straw delignification in anaerobic bioreactors2021

    • Author(s)
      Dumond Louison、Lam Pui Ying、van Erven Gijs、Kabel Mirjam、Mounet Fabien、Grima-Pettenati Jacqueline、Tobimatsu Yuki、Hernandez-Raquet Guillermina
    • Journal Title

      ACS Sustainable Chemistry & Engineering

      Volume: 9 Issue: 5 Pages: 2191-2202

    • DOI

      10.1021/acssuschemeng.0c07817

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Localised laccase activity modulates distribution of lignin polymers in gymnosperm compression wood2021

    • Author(s)
      Hiraide Hideto、Tobimatsu Yuki、Yoshinaga Arata、Lam Pui Ying、Kobayashi Masaru、Matsushita Yasuyuki、Fukushima Kazuhiko、Takabe Keiji
    • Journal Title

      New Phytologist

      Volume: in press Issue: 6 Pages: 2186-2199

    • DOI

      10.1111/nph.17264

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Possible mechanisms for the generation of phenyl glycoside‐type lignin?carbohydrate linkages in lignification with monolignol glucosides2020

    • Author(s)
      Miyagawa Yasuyuki、Tobimatsu Yuki、Lam Pui Ying、Mizukami Takahito、Sakurai Sayaka、Kamitakahara Hiroshi、Takano Toshiyuki
    • Journal Title

      The Plant Journal

      Volume: 104 Issue: 1 Pages: 156-170

    • DOI

      10.1111/tpj.14913

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Arabidopsis R2R3 MYB transcription factor MYB15 is a key regulator of lignin biosynthesis in effector-triggered immunity2020

    • Author(s)
      Kim Seu Ha、Lam Pui Ying、Lee Myoung-Hoon、Jeon Hwi Seong、Tobimatsu Yuki、Park Ohkmae K.
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 583153-583153

    • DOI

      10.3389/fpls.2020.583153

    • NAID

      120007189281

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Identifying transcription factors that reduce wood recalcitrance and improve enzymatic degradation of xylem cell wall in Populus2020

    • Author(s)
      Hori Chiaki、Takata Naoki、Lam Pui Ying、Tobimatsu Yuki、Nagano Soichiro、Mortimer Jenny C.、Cullen Dan
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 22043-22043

    • DOI

      10.1038/s41598-020-78781-6

    • NAID

      120007189282

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] MYB-mediated regulation of lignin biosynthesis in grasses2020

    • Author(s)
      Miyamoto Takuji、Tobimatsu Yuki、Umezawa Toshiaki
    • Journal Title

      Current Plant Biology

      Volume: 24 Pages: 100174-100174

    • DOI

      10.1016/j.cpb.2020.100174

    • NAID

      120006979740

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lignin metabolic engineering in grasses for primary lignin valorization2020

    • Author(s)
      Toshiaki Umezawa, Yuki Tobimatsu, Masaomi Yamamura, Takuji Miyamoto, Yuri Takeda, Taichi Koshiba, Rie Takada, Pui Ying Lam, Shiro Suzuki, Masahiro Sakamoto
    • Journal Title

      Lignin

      Volume: 1 Pages: 30-41

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] How do grasses produce lignin differently from woods: case studies with rice2022

    • Author(s)
      Yuki Tobimatsu
    • Organizer
      The 20th IUFRO Tree Biotech and the 2nd Forest Tree Molecular Biology and Biotechnology (FTMB) Conferenc
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Tools and Methods for the Analysis, Visualization and Structural Elucidation of Lignin2022

    • Author(s)
      Yuki Tobimatsu
    • Organizer
      2022 Lignin Gordon Research Conference
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] How do grasses produce lignin differently from woods: case studies with rice2022

    • Author(s)
      Yuki Tobimatsu
    • Organizer
      2022 Lignin Gordon Research Conference
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] リグニンの多様性と可変性:組換え植物で探る木質の機能と利用へのインパクト2022

    • Author(s)
      飛松裕基
    • Organizer
      第1回リグニン学会特別セミナー「リグニン科学の最前線」
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] NMR法を活用した木質バイオマスの構造・形成・利用研究2022

    • Author(s)
      飛松裕基
    • Organizer
      第22回若手NMR研究会
    • Related Report
      2022 Annual Research Report
    • Invited
  • [Presentation] Lignocellulose characterization of rice mutants deficient in aldehyde dehydrogenases involved in the biosynthesis of cell wall cross-linking ferulates2022

    • Author(s)
      Senri Yamamoto, Yuki Tobimatsu, Osama A. Afifi, Pui Ying Lam, Yuri Takeda-Kimura, Yuriko Osakabe, Keishi Osakabe, Laura E. Bartley, Toshiaki Umezawa
    • Organizer
      IX Cell Wall Research Conference
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distinct alterations of lignin biosynthesis in genome-edited rice mutants deficient in two 4-COUMARATE:COENZYME A LIGASE genes2022

    • Author(s)
      Osama Ahmed Afifi, Yuki Tobimatsu, Pui Ying Lam, Andri Fadillah Martin, Takuji Miyamoto, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa
    • Organizer
      第39回日本植物バイオテクノロジー学会(堺)大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 細胞壁結合型フェルラ酸の形成を抑制した イネ ALDH 変異株のリグノセルロース構造解析2022

    • Author(s)
      山本千莉,飛松裕基,Pui Ying Lam, Osama A. Afifi,木村ゆり,刑部祐里子,刑部敬史,Laura E. Bartley,梅澤俊明
    • Organizer
      第39回日本植物バイオテクノロジー学会(堺)大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Altered flavonoid profile and lignin structure in rice mutants deficient in chalcone synthase (CHS), chalcone isomerase (CHI) and chalcone isomerase-like (CHIL)2022

    • Author(s)
      Pui Ying Lam, Lanxiang Wang, Andy Lui, Toshiaki Umezawa, Yuki Tobimatsu, Clive Lo
    • Organizer
      第39回日本植物バイオテクノロジー学会(堺)大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] フェルラ酸架橋構造及びリグニン芳香核組成を改変したイネ組換え株の細胞壁超分子構造解析2022

    • Author(s)
      山本千莉,Osama A. Afifi,久住亮介,小林加代子,梅澤俊明,飛松裕基
    • Organizer
      細胞壁研究者ネットワーク・第16回定例研究会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Structural Features of Guaiacyl-enriched Lignin Polymers Produced in Rice and Arabidopsis Mutants Deficient in Syringyl Lignin Biosynthesis2022

    • Author(s)
      Ji Pingping,Osama A. Afifi,山本千莉,刑部祐里子,刑部敬史,梅澤俊明,飛松裕基
    • Organizer
      第67回リグニン討論会/リグニン学会第4回年次大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] イネの木化に関わるラッカーゼの探索:候補遺伝子の選定とゲノム編集2022

    • Author(s)
      寺野真季,山本千莉,刑部祐里子,刑部敬史,梅澤俊明,飛松裕基
    • Organizer
      第67回リグニン討論会/リグニン学会第4回年次大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] リグニン芳香核組成及びフェルラ酸架橋構造を改変した組換えイネ株のリグノセルロース超分子構造解析2022

    • Author(s)
      山本千莉、Osama A. Afifi、今井友也、久住亮介、小林加代子、梅澤 俊明、飛松 裕基
    • Organizer
      第72回日本木材学会大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Lignin complexity and flexibility: how and why do grasses produce lignins different from woods?2021

    • Author(s)
      Yuki Tobimatsu
    • Organizer
      2022 World Wood Day Online Symposium and The Fourth IUFRO Forest Products Culture Colloquium
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biosynthesis and bioengineering of tricin-lignin in grass cell walls2021

    • Author(s)
      Pui Ying Lam, Yuki Tobimatsu, Andy C. W. Lui, Lanxiang Wang, Toshiaki Umezawa, Clive Lo
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distinct alterations of lignin biosynthesis in genome-edited rice mutants deficient in two 4-COUMARATE:COENZYME A LIGASE genes2021

    • Author(s)
      Osama Ahmed Afifi, Yuki Tobimatsu, Pui Ying Lam, Andri Fadillah Martin, Takuji Miyamoto, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of aldehyde dehydrogenases in cell-wall feruloylation in grasses2021

    • Author(s)
      Senri Yamamoto, Yuki Tobimatsu, Pui Ying Lam, Yuri Takeda-Kimura, Yuriko Osakabe, Keishi Osakabe, Laura E. Bartley, Toshiaki Umezawa
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Engineering grass cell walls with severe reduction of hydroxycinnamates and lignin for evaluating their structural and physiological roles2021

    • Author(s)
      Nidhi Dwivedi, Senri Yamamoto, Yunjun Zhao, Yuki Tobimatsu, Chang-Jun Liu
    • Organizer
      The 7th International Conference on Plant Cell Wall Biology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] イネにおける細胞壁のフェルラ酸架橋構造形成に寄与するアルデヒドデヒドロゲナーゼの同定2021

    • Author(s)
      山本 千莉, 飛松 裕基, プイイン ラム, 木村(武田) ゆり, 刑部 祐里子, 刑部 敬史, バートレイ ローラ, 梅澤 俊明
    • Organizer
      第38回日本植物バイオテクノロジー学会(つくば)大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Roles of CINNAMATE 4-HYDROXYLASE in grass lignin biosynthesis investigated through characterizations of genome-edited rice mutants2021

    • Author(s)
      SUPATMI SUPATMI, Tobimatsu Yuki, Lam Pui Ying, Afifi Osama, Osakabe Yuriko, Osakabe Keishi, Umezawa Toshiaki
    • Organizer
      第38回日本植物バイオテクノロジー学会(つくば)大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] The role of cytosolic ascorbate peroxidase (APX) in lignin biosynthesis in rice2021

    • Author(s)
      Osama A. Afifi, Yuki Tobimatsu, Yuri Takeda-Kimura, Pui Ying Lam, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa
    • Organizer
      第66回リグニン討論会(リグニン学会第3回年次大会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] Roles of CINNAMATE 4-HYDROXYLASE in grass lignin biosynthesis as studied with genome-edited rice mutants2021

    • Author(s)
      SUPATMI, Tobimatsu Yuki, Lam Pui Ying, Afifi Osama, Osakabe Yuriko, Osakabe Keishi, Umezawa Toshiaki
    • Organizer
      第66回リグニン討論会(リグニン学会第3回年次大会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] In-depth lignin analyses of rice mutants deficient in p-coumaroyl-CoA:monolignol transferases2021

    • Author(s)
      Pui Ying Lam, Yuki Tobimatsu, Laura E. Bartley, Toshiaki Umezawa
    • Organizer
      第66回リグニン討論会(リグニン学会第3回年次大会)
    • Related Report
      2021 Annual Research Report
  • [Presentation] 細胞壁結合型フェルラ酸の形成を抑制したイネALDH変異体のリグノセルロース性状解析2021

    • Author(s)
      山本千莉、飛松裕基、Osama A. Afifi、Pui Ying Lam、梅澤俊明
    • Organizer
      第72回日本木材学会大会(名古屋/岐阜・オンライン)
    • Related Report
      2021 Annual Research Report
  • [Presentation] Generation and characterization of genome-edited rice mutants deficient in CINNAMATE 4-HYDROXYLASE2020

    • Author(s)
      Supatmi, Yuki Tobimatsu, Pui Ying Lam, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa
    • Organizer
      第65回リグニン討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Distinct alterations of lignin biosynthesis in genome-edited rice mutants deficient in two 4-COUMARATE: COENZYME A LIGASE genes2020

    • Author(s)
      Osama A. Afifi, Yuki Tobimatsu, Pui Ying Lam, Andri F. Martin, Takuji Miyamoto, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa
    • Organizer
      第65回リグニン討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] イネ科植物におけるフェルラ酸エステルの生合成に関与するアルデヒドデヒドロゲナーゼ遺伝子の探索2020

    • Author(s)
      山本千莉、飛松裕基、Lam Pui Ying、武田ゆり、刑部祐里子、刑部敬史、Laura E. Bartley、梅澤俊明
    • Organizer
      第65回リグニン討論会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 多様なリグニンモノマー ~リグニンの多様性・複雑性の源~2020

    • Author(s)
      飛松裕基
    • Organizer
      第65回リグニン討論会 特別企画 「リグニンの真の姿に迫る」
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Characterization of genome-edited rice mutants deficient in CINNAMATE 4- HYDROXYLASE genes toward understanding lignin biosynthesis in grasses2020

    • Author(s)
      Supatmi, Yuki Tobimatsu, Pui Ying Lam, Yuriko Osakabe, Keishi Osakabe, Toshiaki Umezawa
    • Organizer
      5th SATREPS conference
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Upregulation of rice lignification with CRISPR/Cas9-mediated targeted mutagenesis2020

    • Author(s)
      Takuji Miyamoto, Rie Takada, Yuri Takeda, Masaomi Yamamura, Yuki Tobimatsu, Shiro Suzuki, Wahyuni, Vincentia Esti Windiastri, Dwi Widyajayanti, Satya Nugroho, Masahiro Sakamoto, Toshiaki Umezawa
    • Organizer
      5th SATREPS conference
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] BAHD acyltransferases responsible for lignin p-coumaroylation in rice cell walls2020

    • Author(s)
      Pui Ying Lam, Yuki Tobimatsu, Shiro Suzuki, Takuto Tanaka, Yuri Takeda, Yuriko Osakabe, Keishi Osakabe, Laura E. Bartley, Toshiaki Umezawa
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 二次壁S2層形成に関与する転写因子の同定2020

    • Author(s)
      高田直樹, 粟野達也, Pui Ying Lam, 鈴木史朗, 飛松裕基, 光田展隆, 朽名夏麿, 山 岸祐介, 谷口亨
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Cellular and subcellular localization of haustorium inducing signals in the haustorium of the parasitic plant Striga hermonthica2020

    • Author(s)
      Songkui Cui, Yuri Takeda, Toshiaki Umezawa, Yuki Tobimatsu, Satoko Yoshida
    • Organizer
      第62回日本植物生理学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] BAHD acyltransferases responsible for lignin p-coumaroylation in rice cell walls2020

    • Author(s)
      Pui Ying Lam, Yuki Tobimatsu, Shiro Suzuki, Takuto Tanaka, Yuri Takeda, Yuriko Osakabe, Keishi Osakabe, Laura E. Bartley, Toshiaki Umezawa
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] フェルラ酸生合成に関与するアルデヒドデヒドロゲナーゼ欠損変異イネの細胞壁性状解析2020

    • Author(s)
      山本千莉, 飛松裕基, プイインラム, 武田ゆり, 刑部祐里子, 刑部敬史, ローラバートレイ, 梅澤俊明
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] S2層形成を制御する転写因子の探索と機能解析2020

    • Author(s)
      高田直樹, 粟野達也, Pui Ying Lam, 鈴木史朗, 飛松裕基, 光田展隆, 朽名夏麿, 山 岸祐介, 谷口亨
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2020 Annual Research Report
  • [Remarks] 京都大学森林代謝機能化学分野研究室ウェブサイト

    • URL

      https://www.rish.kyoto-u.ac.jp/lmsfpm/index.html

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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