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Establishment of basic technology for poultry reproductive engineering by developing germ cell-free chickens

Research Project

Project/Area Number 20H03135
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 42010:Animal production science-related
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Oishi Isao  国立研究開発法人産業技術総合研究所, 生命工学領域, 主任研究員 (50314472)

Co-Investigator(Kenkyū-buntansha) 迎 武紘  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (40803309)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2022: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2021: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2020: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Keywords始原生殖細胞 / ニワトリ / 遺伝子ノックイン / 細胞死誘導 / 生殖工学 / 細胞死 / 生殖細胞 / 遺伝子組換え / ゲノム編集
Outline of Research at the Start

ニワトリは優れた産業動物であり、鶏卵鶏肉から鶏糞に至るまで様々な形で世界中で用いられている。近年、ゲノム編集技術により様々な生物の品種改良が可能となっておりニワトリもその例外ではない。しかし、ニワトリのゲノム編集は極めて複雑で職人的な技術が必要とされ汎用化技術の開発が求められる。本研究では、従来法のボトルネックを解消するニワトリを作出し、ゲノム編集を汎用化する新たな家禽生殖工学基盤技術を確立する。

Outline of Final Research Achievements

We aim to create and utilize germline-free chickens, an important part of chicken reproductive engineering technology. By using germline-free chickens as recipients and transplanting frozen germline cells or genetically engineered cells, we can create germline chimeric chickens with a high donor contribution rate, thereby conserving genetic resources and increasing efficiency in producing genetically engineered individuals. We have attempted to induce germline-specific cell death by creating lines that inducibly express specific genes. Some lines were successful and fertility was confirmed, but other lines still need to be improved. While working to improve the germ cell death induction system, we aim to conserve the genetic resources of chickens and realize an efficient technology for producing genetic modifications.

Academic Significance and Societal Importance of the Research Achievements

ニワトリは産業動物として重要であり、品種改良や遺伝育種も進んでいます。一方、鳥インフルエンザの蔓延や希少品種飼育者の高齢化などで折角の遺伝資源が失われてしまう危機も強まっています。このような状況で生殖工学を用いたニワトリ遺伝資源の保全は重要であり、生殖細胞を持たないニワトリ系統作出は大きな進歩となります。本研究で生殖細胞特異的に遺伝子を制御できる基盤が構築されました。これは生殖細胞を持たないニワトリ開発に向けた足がかりであり、得られた研究資源や知見もとに生殖工学技術に資する系統開発を進めることができます。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (5 results)

All 2023 2022 2021 2020

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results) Presentation (3 results) (of which Int'l Joint Research: 1 results,  Invited: 1 results)

  • [Journal Article] Production and characterization of eggs from hens with ovomucoid gene mutation2021

    • Author(s)
      Mukae Takehiro、Yoshii Kyoko、Watanobe Takuma、Tagami Takahiro、Oishi Isao
    • Journal Title

      Poultry Science

      Volume: 100 Issue: 2 Pages: 452-460

    • DOI

      10.1016/j.psj.2020.10.026

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Production of Recombinant Monoclonal Antibodies in the Egg White of Gene-Targeted Transgenic Chickens2020

    • Author(s)
      Mukae Takehiro、Okumura Sho、Watanobe Takuma、Yoshii Kyoko、Tagami Takahiro、Oishi Isao
    • Journal Title

      Genes

      Volume: 12 Issue: 1 Pages: 38-38

    • DOI

      10.3390/genes12010038

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] ゲノム編集ニワトリ由来IgG抗体を用いた低コスト抗原検出技術の開発2023

    • Author(s)
      迎 武紘
    • Organizer
      日本薬学会 第143年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] ゲノム編集ニワトリ生産系を用いた糖鎖改変糖タンパク質生産技術2022

    • Author(s)
      迎 武紘、吉井 京子、大石 勲
    • Organizer
      日本薬学会第142回年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Technologies of chicken genome modification and possible applications for preservation of avian genetic resources2021

    • Author(s)
      Isao Oishi
    • Organizer
      Avian biodiversity: from developmental biology to preservation of endangered species, e-ASIA JRP workshop
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited

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Published: 2020-04-28   Modified: 2024-01-30  

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