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Molecular mechanism of cellular reprogramming in plants

Research Project

Project/Area Number 20H03284
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Review Section Basic Section 44030:Plant molecular biology and physiology-related
Research InstitutionInstitute of Physical and Chemical Research

Principal Investigator

Sugimoto Keiko  国立研究開発法人理化学研究所, 環境資源科学研究センター, チームリーダー (30455349)

Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥17,680,000 (Direct Cost: ¥13,600,000、Indirect Cost: ¥4,080,000)
Fiscal Year 2022: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2021: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2020: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Keywords細胞リプログラミング / 器官再生 / 植物ホルモン / ストレス応答 / シロイヌナズナ
Outline of Research at the Start

傷害ストレスは細胞リプログラミングを誘導し、器官再生を促進するが、その分子機構は断片的にしか理解されていない。本研究ではストレス応答に関与する転写因子の下流標的遺伝子を同定し、機能を解析することで、傷害ストレスがいかに細胞リプログラミングを誘導するかを解明する。またシロイヌナズナの葉肉プロトプラストからの再生系を用いて、傷害誘導性の転写制御ネットワークが最終分化した細胞からのリプログラミングにも関与するかどうかを検証し、植物細胞の分化転換機構の包括的な理解を進める。

Outline of Final Research Achievements

Wounding stress induces cellular reprogramming in regeneration but the molecular mechanisms underlying this control remain largely unknown. In this study we aimed at uncovering the transcriptional mechanisms that regulate wound-induced cellular reprogramming in plants. Using the Arabidopsis tissue culture system, we revealed the early transcriptional cascade activated by wounding. We also showed that the activity of this early transcriptional network is regulated by SUMO-mediated post-translational mechanisms. Using the Arabidopsis mesophyll protoplast system, we also demonstrated that the part of the wound-induced transcriptional cascade is also required for the reprogramming from differentiated plant cells.

Academic Significance and Societal Importance of the Research Achievements

CRISPR-Cas9法等のゲノム編集技術が急速に発展するなかでも、多くの有用植物において形質転換後の再生効率の低さが品質向上技術確立の大きなボトルネックになっている。本研究は細胞リプログラミングの新たな制御機構を明らかにするものであり、再生しにくい植物の再生効率を向上させるための分子組織培養法の確立に貢献することが期待される。また、分化した植物体細胞のリプログラミングを定量的に評価する実験系を始めて立ち上げたことで、分化細胞からのリプログラミングを制御する分子機構を解明することを可能にした点に本研究の学術的意義がある。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • Research Products

    (41 results)

All 2023 2022 2021 2020 Other

All Int'l Joint Research (3 results) Journal Article (24 results) (of which Int'l Joint Research: 15 results,  Peer Reviewed: 22 results,  Open Access: 17 results) Presentation (11 results) (of which Int'l Joint Research: 9 results,  Invited: 10 results) Remarks (2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] VIB-UGent(ベルギー)

    • Related Report
      2022 Annual Research Report
  • [Int'l Joint Research] VIB-UGENT(ベルギー)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] VIB(ベルギー)

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Cytokinin signaling promotes root hair growth by directly regulating RSL4 expression2023

    • Author(s)
      Takatsuka Hirotomo、Sasaki Anna、Takahashi Naoki、Shibata Michitaro、Sugimoto Keiko、Tanaka Maho、Seki Motoaki、Umeda Masaaki
    • Journal Title

      Journal of Experimental Botany

      Volume: - Issue: 12 Pages: 3579-3594

    • DOI

      10.1093/jxb/erad091

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis histone H3 lysine 9 methyltransferases KYP/SUVH5/6 are involved in leaf development by interacting with AS1-AS2 to repress KNAT1 and KNAT22023

    • Author(s)
      Hung Fu-Yu、Feng Yun-Ru、Hsin Kuan-Ting、Shih Yuan-Hsin、Chang Chung-Han、Zhong Wenjian、Lai You-Cheng、Xu Yingchao、Yang Songguang、Sugimoto Keiko、Cheng Yi-Sheng、Wu Keqiang
    • Journal Title

      Communications Biology

      Volume: 6 Issue: 1 Pages: 219-219

    • DOI

      10.1038/s42003-023-04607-6

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] How do plants reprogramme the fate of differentiated cells?2023

    • Author(s)
      Morinaka Hatsune、Sakamoto Yuki、Iwase Akira、Sugimoto Keiko
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 74 Pages: 102377-102377

    • DOI

      10.1016/j.pbi.2023.102377

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Mechanisms of Plant Regeneration from Differentiated Cells: Approaches from Historical Tissue Culture Systems2022

    • Author(s)
      Morinaka Hatsune、Coleman Duncan、Sugimoto Keiko、Iwase Akira
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 3 Pages: 297-304

    • DOI

      10.1093/pcp/pcac172

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] MYB3R-SCL28-SMR module with a role in cell size control negatively regulates G2 progression in <i>Arabidopsis</i>2022

    • Author(s)
      Takatsuka Hirotomo、Nomoto Yuji、Yamada Kesuke、Mineta Keito、Breuer Christian、Ishida Takashi、Yamagami Ayumi、Sugimoto Keiko、Nakano Takeshi、Ito Masaki
    • Journal Title

      Plant Signaling &amp; Behavior

      Volume: e2153209 Issue: 1 Pages: 1-5

    • DOI

      10.1080/15592324.2022.2153209

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Auxin-Induced WUSCHEL-RELATED HOMEOBOX13 Mediates Asymmetric Activity of Callus Formation upon Cutting2022

    • Author(s)
      Tanaka Hayato、Hashimoto Naoki、Kawai Satomi、Yumoto Emi、Shibata Kyomi、Tameshige Toshiaki、Yamamoto Yuma、Sugimoto Keiko、Asahina Masashi、Ikeuchi Momoko
    • Journal Title

      Plant and Cell Physiology

      Volume: 64 Issue: 3 Pages: 305

    • DOI

      10.1093/pcp/pcac146

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Transcriptional activation of auxin biosynthesis drives developmental reprogramming of differentiated cells2022

    • Author(s)
      Sakamoto Yuki、Kawamura Ayako、Suzuki Takamasa、Segami Shoji、Maeshima Masayoshi、Polyn Stefanie、De Veylder Lieven、Sugimoto Keiko
    • Journal Title

      The Plant Cell

      Volume: 34 Issue: 11 Pages: 4348-4365

    • DOI

      10.1093/plcell/koac218

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Trihelix transcription factors GTL1 and DF1 prevent aberrant root hair formation in an excess nutrient condition2022

    • Author(s)
      Shibata Michitaro、Favero David S.、Takebayashi Ryu、Takebayashi Arika、Kawamura Ayako、Rymen Bart、Hosokawa Yoichiroh、Sugimoto Keiko
    • Journal Title

      New Phytologist

      Volume: 235 Issue: 4 Pages: 1426-1441

    • DOI

      10.1111/nph.18255

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Topoisomerase VI participates in an insulator-like function that prevents H3K9me2 spreading2022

    • Author(s)
      Meteignier Louis-Valentin、Lecampion Cecile、Velay Florent、Vriet Cecile、Dimnet Laura、Rougee Martin、Breuer Christian、Soubigou-Taconnat Ludivine、Sugimoto Keiko、Barneche Fredy、Laloi Christophe
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 27

    • DOI

      10.1073/pnas.2001290119

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A hierarchical transcriptional network activates specific CDK inhibitors that regulate G2 to control cell size and number in Arabidopsis2022

    • Author(s)
      Nomoto Yuji、Takatsuka Hirotomo、Yamada Kesuke、Suzuki Toshiya、Suzuki Takamasa、Huang Ying、Latrasse David、An Jing、Gombos Magdolna、Breuer Christian、Ishida Takashi、Maeo Kenichiro、Imamura Miyu、Yamashino Takafumi、Sugimoto Keiko、Magyar Zolt?n、B?gre L?szl?、Raynaud C?cile、Benhamed Moussa、Ito Masaki
    • Journal Title

      Nature Communications

      Volume: 13 Issue: 1 Pages: 1660-1660

    • DOI

      10.1038/s41467-022-29316-2

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] SUPPRESSOR OF PHYTOCHROME B-4 #3 reduces the expression of PIF-activated genes and increases expression of growth repressors to regulate hypocotyl elongation in short days2022

    • Author(s)
      Jacques Caitlin N.、Favero David S.、Kawamura Ayako、Suzuki Takamasa、Sugimoto Keiko、Neff Michael M.
    • Journal Title

      BMC Plant Biology

      Volume: 22 Issue: 1 Pages: 399-399

    • DOI

      10.1186/s12870-022-03737-z

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Warm Temperature Promotes Shoot Regeneration in <i>Arabidopsis thaliana</i>2022

    • Author(s)
      Lambolez Alice、Kawamura Ayako、Takahashi Tatsuya、Rymen Bart、Iwase Akira、Favero David S、Ikeuchi Momoko、Suzuki Takamasa、Cortijo Sandra、Jaeger Katja E、Wigge Philip A、Sugimoto Keiko
    • Journal Title

      Plant and Cell Physiology

      Volume: ー Issue: 5 Pages: 618-634

    • DOI

      10.1093/pcp/pcac017

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Plant development meets climate emergency ? it's time to plant an apple tree2022

    • Author(s)
      Sugimoto Keiko、Nowack Moritz K.
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 65 Pages: 102175-102175

    • DOI

      10.1016/j.pbi.2022.102175

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues2022

    • Author(s)
      Serivichyaswat Phanu T.、Bartusch Kai、Leso Martina、Musseau Constance、Iwase Akira、Chen Yu、Sugimoto Keiko、Quint Marcel、Melnyk Charles W.
    • Journal Title

      Development

      Volume: 149 Issue: 5

    • DOI

      10.1242/dev.200079

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 4-phenylbutyric acid promotes plant regeneration as an auxin by being converted to phenylacetic acid via an IBR3-independent pathway2022

    • Author(s)
      Iwase A, Takebayashi A, Aoi Y, Favero DS, Watanabe S, Seo M, Kasahara H, Sugimoto K
    • Journal Title

      Plant Biotechnology

      Volume: in press

    • NAID

      130008161269

    • Related Report
      2021 Annual Research Report
  • [Journal Article] WIND transcription factors orchestrate wound-induced callus formation, vascular reconnection and defense response in Arabidopsis2021

    • Author(s)
      Iwase A, Kondo Y, Laohavisit A, Takebayashi A, Ikeuchi M, Matsuoka K, Asahina M, Mitsuda N, Shirasu K, Fukuda H, Sugimoto K
    • Journal Title

      New Phytologist

      Volume: 232 Issue: 2 Pages: 734

    • DOI

      10.1111/nph.17594

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fifteen compelling open questions in plant cell biology2021

    • Author(s)
      Roeder Adrienne H K、Otegui Marisa S、Dixit Ram、Anderson Charles T、Faulkner Christine、Zhang Yan、Harrison Maria J、Kirchhelle Charlotte、Goshima Gohta、Coate Jeremy E、Doyle Jeff J、Hamant Olivier、Sugimoto Keiko、Dolan Liam、Meyer Heather、Ehrhardt David W、Boudaoud Arezki、Messina Carlos
    • Journal Title

      The Plant Cell

      Volume: 34 Issue: 1 Pages: 72-102

    • DOI

      10.1093/plcell/koab225

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Transcriptome Dynamics of Epidermal reprogramming during direct shoot regeneration in Torenia fournieri2021

    • Author(s)
      Hatsune Morinaka, Akihito Mamiya, Hiroaki Tamaki, Akitoshi Iwamoto, Takamasa Suzuki, Ayako Kawamura, Momoko Ikeuchi, Akira Iwase, Tetsuya Higashiyama, Keiko Sugimoto, Munetaka Sugiyama
    • Journal Title

      Plant & Cell Physiology

      Volume: 62 Issue: 8 Pages: 1335-1354

    • DOI

      10.1093/pcp/pcab101

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Spatial transcriptional signatures define margin morphogenesis along the proximal-distal and medio-lateral axes in tomato (Solanum lycopersicum) leaves2020

    • Author(s)
      Martinez Ciera C、Li Siyu、Woodhouse Margaret R、Sugimoto Keiko、Sinha Neelima R
    • Journal Title

      The Plant Cell

      Volume: 33 Pages: 44-65

    • DOI

      10.1093/plcell/koaa012

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Molecular pathways regulating elongation of aerial plant organs: a focus on light, the circadian clock, and temperature2020

    • Author(s)
      Favero David S.、Lambolez Alice、Sugimoto Keiko
    • Journal Title

      The Plant Journal

      Volume: 105 Issue: 2 Pages: 392-420

    • DOI

      10.1111/tpj.14996

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Common Mechanisms of Developmental Reprogramming in Plants-Lessons From Regeneration, Symbiosis, and Parasitism2020

    • Author(s)
      Ichihashi Yasunori、Hakoyama Tsuneo、Iwase Akira、Shirasu Ken、Sugimoto Keiko、Hayashi Makoto
    • Journal Title

      Frontiers in Plant Science

      Volume: 11 Pages: 1084-1084

    • DOI

      10.3389/fpls.2020.01084

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] How do plants transduce wound signals to induce tissue repair and organ regeneration?2020

    • Author(s)
      Ikeuchi Momoko、Rymen Bart、Sugimoto Keiko
    • Journal Title

      Current Opinion in Plant Biology

      Volume: 57 Pages: 72-77

    • DOI

      10.1016/j.pbi.2020.06.007

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The SUMO E3 ligase SIZ1 negatively regulates shoot regeneration2020

    • Author(s)
      Duncan Coleman, Ayako Kawamura, Momoko Ikeuchi, David Seth Favero, Alice Lambolez, Bart Rymen, Akira Iwase, Takamasa Suzuki, Keiko Sugimoto
    • Journal Title

      Plant Physiology

      Volume: 184 Issue: 1 Pages: 330-344

    • DOI

      10.1104/pp.20.00626

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 傷口からの再生 : 植物の細胞リプログラミング2020

    • Author(s)
      池内桃子、杉本慶子
    • Journal Title

      遺伝 : 生物の科学

      Volume: 5 Pages: 568-573

    • Related Report
      2020 Annual Research Report
  • [Presentation] Epigenetic control of cellular reprogramming in plant regeneration2022

    • Author(s)
      Keiko Sugimoto
    • Organizer
      Cold Spring Harbor Asia Conference, Awaji, Japan
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Control of cell reprogramming in plant regeneration2022

    • Author(s)
      Keiko Sugimoto
    • Organizer
      FASEB meeting, Boston, USA
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] How do plants feel the damage to protect and rebuild themselves?2022

    • Author(s)
      Keiko Sugimoto
    • Organizer
      EMBO Workshop, Montpellier, France
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Control of cell reprogramming in plant regeneration2022

    • Author(s)
      Keiko Sugimoto
    • Organizer
      Israeli Center for Genome Editing, online
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物の傷害修復応答2021

    • Author(s)
      杉本 慶子
    • Organizer
      日本植物生理学会年会シンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 植物の環境レジリエンスを支える傷害修復機構2021

    • Author(s)
      杉本 慶子
    • Organizer
      日本分子生物学会年会シンポジウム
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Control of cellular reprogramming in plant regeneration.2021

    • Author(s)
      Keiko Sugimoto
    • Organizer
      Triangle Plant Molecular Biology Consortium at North Carolina Biotechnology Center
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Control of cellular reprogramming in plant regeneration.2021

    • Author(s)
      Keiko Sugimoto
    • Organizer
      Plant Regeneration Workshop
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Control of cellular reprogramming in plant regeneration2020

    • Author(s)
      Keiko Sugimoto
    • Organizer
      Japan Society of Developmental Biologists, September 2020 online
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Control of cellular reprogramming in plant regeneration2020

    • Author(s)
      Keiko Sugimoto
    • Organizer
      Sainsbury Laboratory Symposium 2020 on Pluripotency in Plant Development online
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanism of cellular reprogramming in plants2020

    • Author(s)
      Keiko Sugimoto
    • Organizer
      ASPB Plantae virtual seminar series, April 2020 online
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] Cell Function Research Team

    • URL

      http://cellfunction.riken.jp/

    • Related Report
      2022 Annual Research Report 2021 Annual Research Report
  • [Remarks] 細胞機能研究チーム

    • URL

      http://cellfunction.riken.jp/index.html

    • Related Report
      2020 Annual Research Report
  • [Patent(Industrial Property Rights)] 植物の再生効率を向上する新技術2020

    • Inventor(s)
      杉本慶子
    • Industrial Property Rights Holder
      杉本慶子
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-054721
    • Filing Date
      2020
    • Related Report
      2020 Annual Research Report

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Published: 2020-04-28   Modified: 2024-01-30  

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