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Elucidation of Factors to Fruit Set Failure in Self-Pollination of Tetraploid Rocoto Peppers Obtaining Self-Compatibility.

Research Project

Project/Area Number 20K06017
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 39030:Horticultural science-related
Research InstitutionKyushu University

Principal Investigator

Miyajima Ikuo  九州大学, 熱帯農学研究センター, 学術特定研究者 (20182024)

Co-Investigator(Kenkyū-buntansha) 水ノ江 雄輝  九州大学, 農学研究院, 助教 (50759206)
Project Period (FY) 2020-04-01 – 2024-03-31
Project Status Completed (Fiscal Year 2023)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords四倍体 / ロコトトウガラシ / 高温障害 / 自家不和合性 / 自家和合性 / コルヒチン処理 / 花粉発芽 / 果実着色 / 倍数性 / 自家受粉
Outline of Research at the Start

南米アンデス原産のロコトトウガラシ(ロコト)はピーマンに似た果形をもつ辛みの強いトウガラシだが,果肉に水分が多く新たな利用が期待できるトウガラシである.ただ,ロコトは自家不和合性を示すためその打破が必要であった.我々はロコト種子をコルヒチン処理することで自家和合性をもつ四倍体の獲得に成功した.しかし,四倍体ロコトの自家受粉での着果率は他家受粉に比べて有意に低かった.本研究では四倍体ロコトの自家受粉にみられる着果不良の要因解明とその打破を目的とした.

Outline of Final Research Achievements

Rocoto pepper (rocoto), native to the Andes region of South America, is a new pungent pepper with high water content in its pericarp, which allows the use of fresh fruits. The problem was to overcome the self-incompatibility of this species in order to use it as a horticultural crop. In contrast, tetraploid rocoto obtained by colchicine treatment was found to be self-compatibility, but the low self-pollination rate was a new problem.
The results of this study revealed that pollen germination of rocoto was suppressed under high temperature conditions, which was considered to be the main reason for the low self-pollination rate. The tetraploid rocoto showed a self-pollination rate of nearly 65% under appropriate temperature conditions, indicating the practicality of tetraploid rocoto.

Academic Significance and Societal Importance of the Research Achievements

本研究により,四倍体ロコトの自家受粉での着果不良の主な要因は,自家不和合性に関するSハプロタイプをヘテロ接合でもつ花粉だけしか受精にかかわれない(受精可能な花粉量が少ない)ためではなく,環境要因(高温)である可能性が示された.
ロコトは冷涼な気候を好むので,耕作放棄地の多い我が国の中山間地での栽培に適した作物といえる.さらに,四倍体ロコトの新鮮果実は果皮が厚くジューシーなため,ドレッシングや酢漬け(ピクルス)等の加工用トウガラシとしての利用が期待される.したがって,農産物の6次産業化を目指す地域では今後注目される作物と思われる.

Report

(5 results)
  • 2023 Annual Research Report   Final Research Report ( PDF )
  • 2022 Research-status Report
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (3 results)

All 2024 2022

All Journal Article (1 results) (of which Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Rocoto: Ancient and Juicy Hot Peppers2024

    • Author(s)
      宮島郁夫
    • Journal Title

      Foods & Food Ingredients Journal of Japan

      Volume: 229 Issue: 2 Pages: 084-090

    • DOI

      10.34457/ffij.229.2_084

    • ISSN
      0919-9772, 2436-5998
    • Year and Date
      2024-04-01
    • Related Report
      2023 Annual Research Report
    • Open Access
  • [Presentation] 早期に収穫した四倍体ロコトトウガラシ(Capsicum pubescens)の緑色果実の着色に対する温度と光照射の効果2022

    • Author(s)
      宮島郁夫・藤原菜々子・水ノ江雄輝・尾崎行生
    • Organizer
      令和4年度園芸学会秋季大会
    • Related Report
      2022 Research-status Report
  • [Presentation] コルヒチン処理により得られた四倍体ロコトトウガラシ(Capsicum pubescens)の 自家和合系統における果実形質2022

    • Author(s)
      藤原菜々子・水ノ江雄輝・宮島郁夫・尾崎行生
    • Organizer
      令和4年度園芸学会春季大会
    • Related Report
      2021 Research-status Report

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Published: 2020-04-28   Modified: 2025-01-30  

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