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Is the mass mortality of pearl oyster in the summer of 2019 a Tenacibaculosis ?

Research Project

Project/Area Number 20K06203
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 40030:Aquatic bioproduction science-related
Research InstitutionUniversity of Toyama

Principal Investigator

SAKATOKU Akihiro  富山大学, 学術研究部理学系, 講師 (20713142)

Co-Investigator(Kenkyū-buntansha) 一色 正  三重大学, 生物資源学研究科, 教授 (30378319)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2021: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords真珠 / アコヤガイ / 軟体部萎縮症 / 殻黒変病 / Vibrio sp. MA3株 / Tenacibaculum sp. Pbs-1株 / 細菌感染症 / Vibrio sp. MA3 / ヘモリシン / Tenacibaculum sp. Pbs-1 / LAMP法 / 特異検出法
Outline of Research at the Start

2019年夏季, 全国のアコヤ真珠養殖場で, 母貝の外套膜が委縮する“原因不明の大量死”が発生した。これまでの予備的試験から, 全ての病貝から滑走細菌Tenacibaculum様細菌が単離されたことから, 大量死の原因は本菌による感染症ではないかと考えられた。その一方で, 申請者らは, “アコヤガイ殻黒変病”の原因がTenacibaculum sp. Pbs-1株の感染症であることを明らかにしている。そこで本研究では, 分離菌株を用いた感染実験を行い, 2019年のアコヤガイ大量死の原因を究明するとともに, Pbs-1株の特異検出法も開発する。

Outline of Final Research Achievements

The purpose of this study that we describe the isolation and characterization of a pathogenic bacterium as a first step towards resolving the recent disease outbreaks in cultured pearl oysters, as characterized by extreme atrophy of their soft tissues. In addition, we develop a specific detection method for Tenacibaculum sp. Pbs-1 associated with black-spot shell disease in akoya pearl.
We isolated a Vibrio sp. strain MA3 from the mantle and other soft tissues of diseased pearl oysters. Strain MA3 possessed a pathogenic factor, hemolysin (Vhe1), of vibriosis. Moreover, the results of the infection-challenge experiment in this study provide evidence that strain MA3 was one of the putative causal agents of mass mortalities of farmed pearl oysters in Japan in the summers of 2019 and 2020. We developed and evaluated a rapid, specific, and sensitive loop mediated isothermal amplification assay to detect Tenacibaculum sp. Pbs-1 associated with black spot shell disease.

Academic Significance and Societal Importance of the Research Achievements

日本の養殖アコヤ真珠は, 世界的に非常に評価が高く, 日本の水産物年間輸出額の11% (約330億円) を超える重要な輸出産業となっている。しかし近年, 母貝の殻内側が著しく黒変し, 低品質の真珠形成や死亡を引き起こす‘殻黒変病’と, 外套膜が極端に委縮し死亡する‘軟体部萎縮症’が全国の真珠養殖場で大きな問題となっている。これらの疾病の全容解明は, 緊急性を要し, 日本が誇る真珠養殖産業の持続・発展に不可欠の課題である。本研究成果は, その解決に向けた第一歩であり, 学術的・社会的意義は高いと考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (14 results)

All 2023 2022 2021

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (8 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] Development and evaluation of a rapid, specific, and sensitive loop-mediated isothermal amplification assay to detect Tenacibaculum sp. strain pbs-1 associated with black-spot shell disease in Akoya pearl oysters2022

    • Author(s)
      Sakatoku Akihiro、Suzuki Takaya、Tatamiya Yuri、Seki Makoto、Tanaka Daisuke、Nakamura Shogo、Isshiki Tadashi
    • Journal Title

      Archives of Microbiology

      Volume: 205 Issue: 1 Pages: 43-43

    • DOI

      10.1007/s00203-022-03384-w

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a method of sensitively and specifically detecting a Vibrio sp. strain MA3 associated with mass mortalities of the pearl oyster Pinctada fucata martensii using quantitative PCR.2022

    • Author(s)
      Kaito Hatano, Akihiro Sakatoku, Daisuke Tanaka, Shoki Tanaka, Tadashi Isshiki, Nobuo Suzuki.
    • Journal Title

      International Aquatic Research

      Volume: 14 Pages: 241-250

    • Related Report
      2022 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation and characterization of a Vibrio sp. strain MA3 associated with mass mortalities of the pearl oyster Pinctada fucata.2021

    • Author(s)
      Akihiro Sakatoku, Kaito Hatano, Shoki Tanaka, Tadashi Isshiki
    • Journal Title

      Archives of Microbiology

      Volume: 203 Issue: 8 Pages: 5267-5273

    • DOI

      10.1007/s00203-021-02457-6

    • Related Report
      2021 Research-status Report
    • Peer Reviewed
  • [Presentation] アコヤガイに殻黒変病を引き起こすTenacibaculum sp. Pbs-1株を 迅速・特異的・高感度に検出するLAMP法の開発2023

    • Author(s)
      鈴木貴也, 畳谷優莉, 関 誠, 田中大祐, 中村省吾, 一色 正, 酒徳昭宏
    • Organizer
      令和5年度日本水産学会 春季大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] 大量死したアコヤガイから単離された細菌を特異的に検出する定量PCR法の開発2023

    • Author(s)
      端野開都, 酒徳昭宏, 田中大祐, 田中翔稀, 一色正, 鈴木信雄
    • Organizer
      第57回日本水環境学会年会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Vibrio sp. strain MA3 involves for the mass mortality of the summer in the pearl oyster, Pinctada fucata.2022

    • Author(s)
      Hatano K, Sakatoku A, Tanaka S, Isshiki T, Suzuki N
    • Organizer
      Joint Usage/Joint Research on Transboundary Pollution and its Impact on Social Environment
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 新規に単離されたMA3 株によるアコヤガイ感染症に関する研究2022

    • Author(s)
      端野開都, 一色 正, 田中翔稀, 酒徳昭宏, 鈴木信雄
    • Organizer
      日本動物学会中部支部大会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Investigation about the Vibrio sp. strain MA3 with mass mortality of pearl oyster occurred in the summer.2022

    • Author(s)
      Hatano K, Sakatoku A, Tanaka S, Isshiki T, Suzuki N
    • Organizer
      The 6th International Exchange Seminar of Zoology
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アコヤガイの大量死に関連するVibrio属細菌の解析2022

    • Author(s)
      端野開都, 一色 正, 田中翔稀, 酒徳昭宏, 鈴木信雄
    • Organizer
      日本水産学会春季大会
    • Related Report
      2021 Research-status Report
  • [Presentation] 高水温期に発生するアコヤガイ大量死の原因は細菌感染によるものか?2021

    • Author(s)
      酒徳昭宏, 一色正
    • Organizer
      アコヤガイのへい死に係る情報交換会
    • Related Report
      2021 Research-status Report
    • Invited
  • [Presentation] Isolation, characterization, and detection of a Vibrio sp. strain associated with mass mortalities of the pearl oyster Pinctada fucata.2021

    • Author(s)
      Hatano, K., Sakatoku, A., Tanaka, S., Isshiki, T. and Suzuki, N.
    • Organizer
      金沢大学環日本海域環境研究センター国際シンポジウム
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Patent(Industrial Property Rights)] アコヤガイ殻黒変病原因細菌を検出するためのプライマーセット、方法、及びキット2022

    • Inventor(s)
      酒徳昭宏, 一色正
    • Industrial Property Rights Holder
      富山大学, 三重大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-101950
    • Filing Date
      2022
    • Related Report
      2022 Annual Research Report
  • [Patent(Industrial Property Rights)] アコヤガイの大量斃死を引き起こした病原菌の高感度・高精度の検出法2022

    • Inventor(s)
      鈴木信雄, 端野開都, 酒徳昭宏, 一色正
    • Industrial Property Rights Holder
      金沢大学, 富山大学, 三重大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2022-072792
    • Filing Date
      2022
    • Related Report
      2022 Annual Research Report
  • [Patent(Industrial Property Rights)] アコヤ貝殻黒変病原因細菌の特異的検出法及びそのためのキット2021

    • Inventor(s)
      酒徳 昭宏 (富山大学)、一色 正(三重大学)
    • Industrial Property Rights Holder
      国立大学法人富山大学、国立大学法人三重大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2021-021128
    • Filing Date
      2021
    • Related Report
      2020 Research-status Report

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Published: 2020-04-28   Modified: 2024-01-30  

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