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Identification of chondroitin sulfate isotypes with adipocyte differentiation inhibitory activity, elucidation of their functions, and regulation of fat mass

Research Project

Project/Area Number 20K06411
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 42020:Veterinary medical science-related
Research InstitutionKyushu University (2022)
Tottori University (2020-2021)

Principal Investigator

HOSAKA Yoshinao  九州大学, 農学研究院, 教授 (00337023)

Co-Investigator(Kenkyū-buntansha) 田村 純一  鳥取大学, 農学部, 教授 (30221401)
割田 克彦  鳥取大学, 農学部, 教授 (40452669)
北村 直樹  鳥取大学, 農学部, 准教授 (80301951)
Project Period (FY) 2020-04-01 – 2023-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2022: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2021: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords脂肪細胞分化 / コンドロイチン硫酸 / 糖鎖量 / 糖鎖の構成割合 / 糖鎖長 / 3T3-L1 / 細胞外マトリックス / 脂肪細胞 / 細胞分化 / 脂肪 / グリコサミノグリカン / 硫酸化パターン / 脂肪分化 / CSサブタイプ(型) / 糖鎖 / 分化 / chst
Outline of Research at the Start

脂肪細胞分化抑制活性が最も高いCS種を同定し、その後、分化過程でのCSおよびその合成を担うChstの挙動を、細胞および生体レベルで解明する。さらには、Chstの発現量を、細胞および生体レベルで調節することで、生体の脂肪量の制御を試みる。これらから、脂肪細胞分化でのChstの機能を、細胞および生体レベルで明らかにする。

Outline of Final Research Achievements

Focusing on the relationship between adipocyte differentiation and chondroitin sulfate (CS), the following experiments were conducted. 1) Induced the differentiation of progenitor adipocyte 3T3-L1 into adipocytes in a medium containing CS, and attempted to identify CS species with high adipogenic differentiation inhibitory activity. As a result, C type rich CS showed strong inhibitory activity against adipogenesis. 2) Analyzed in detail the changes in CS chains before and after differentiation into adipocytes, and found that the amount of chains, the CS species constituting them, and their proportions changed. Furthermore, the average chain length also became shorter after differentiation, indicating that qualitative and quantitative changes in the CSs surrounding the cells occurred with the progress of adipocyte differentiation.

Academic Significance and Societal Importance of the Research Achievements

細胞外マトリックスとして存在するCSが、その質や量(構成する糖鎖の種類、割合、糖鎖長など)を絶妙に変化させて、脂肪細胞の分化を制御していることを本課題で、初めて詳細に確認し、報告した。得られた成果の応用、すなわち、CSの量や質を部位・時期特異的に変化させる技術を開発することで、将来、脂肪細胞の分化抑制、すなわち脂肪量の人為的制御が可能になると考えられる。

Report

(4 results)
  • 2022 Annual Research Report   Final Research Report ( PDF )
  • 2021 Research-status Report
  • 2020 Research-status Report
  • Research Products

    (8 results)

All 2023 2022 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 3 results) Remarks (2 results)

  • [Journal Article] Compositional analysis of chondroitin/dermatan sulfate in rhesus monkeys (Macaca mulatta)2022

    • Author(s)
      Cahyadi Danang Dwi、Okamoto Munehiro、Warita Katsuhiko、Takeda-Okuda Naoko、Tamura Jun-ichi、Hosaka Yoshinao Z
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: - Pages: 811-818

    • DOI

      10.1093/bbb/zbac057

    • Related Report
      2022 Annual Research Report 2021 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Quantitative and qualitative analysis of chondroitin/dermatan sulfate in 3T3-L1 cells before and after induction of adipogenesis2023

    • Author(s)
      Danang Dwi Cahyadi, Katsuhiko Warita, Naoko Takeda-Okuda, Jun-ichi Tamura, Yoshinao Z. Hosaka
    • Organizer
      AJIVE Symposium 2023
    • Related Report
      2022 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Adipose tissue properties in the low estrogen state and high fat diet-induced obesity mice2023

    • Author(s)
      Cahyadi Danang Dwi、割田 克彦、保坂 善真
    • Organizer
      日本解剖学会第76回中国/四国支部学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Adipose Tissue Properties in the Low Estrogen State and High Fat Diet-Induced Obesity Mice2023

    • Author(s)
      Cahyadi Danang Dwi、割田 克彦、保坂 善真
    • Organizer
      第165回日本獣医学会学術集会
    • Related Report
      2022 Annual Research Report
  • [Presentation] Compositional analysis of glycosaminoglycans from several tissues in rhesus monkey (Macaca mulatta)2022

    • Author(s)
      Danang Dwi Cahyadi, Munehiro Okamoto, Katsuhiko Warita, Naoko Takeda-Okuda, Jun-ichi Tamura, Yoshinao Z. Hosaka
    • Organizer
      日本農芸化学会 2022京都大会(オンライン)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Presentation] Compositional Analysis of Chondroitin/Dermatan Sulfate in Rhesus Monkeys (Macaca mulatta)2022

    • Author(s)
      Danang Dwi Cahyadi, Munehiro Okamoto, Katsuhiko Warita, Naoko Takeda-Okuda, Jun-ichi Tamura, Yoshinao Z. Hosaka
    • Organizer
      6th AJIVE Symposium - 2022(オンライン)
    • Related Report
      2021 Research-status Report
    • Int'l Joint Research
  • [Remarks] 鳥取大学農学部

    • URL

      http://muses.muses.tottori-u.ac.jp/about/staff/

    • Related Report
      2021 Research-status Report 2020 Research-status Report
  • [Remarks] Researchmap

    • URL

      https://researchmap.jp/Hosaka4470

    • Related Report
      2021 Research-status Report 2020 Research-status Report

URL: 

Published: 2020-04-28   Modified: 2024-01-30  

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