尿路結石マトリックス蛋白オステオポンチンを標的としたマクロファージ貪食機序の解明

• Project/Area Number: 20K09562
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 56030:Urology-related
• Research Institution: Nagoya City University
• Principal Investigator: 岡田 淳志 名古屋市立大学, 医薬学総合研究院(医学), 准教授 (70444966)
• Co-Investigator(Kenkyū-buntansha): 田口 和己 名古屋市立大学, 医薬学総合研究院(医学), 講師 (00595184) ; 郡 健二郎 名古屋市立大学, その他部局等, 学長 (30122047) ; 安藤 亮介 名古屋市立大学, 医薬学総合研究院(医学), 教授 (30381867) ; 松永 民秀 名古屋市立大学, 医薬学総合研究院(薬学), 教授 (40209581) ; 安井 孝周 名古屋市立大学, 医薬学総合研究院(医学), 教授 (40326153) ; 濱本 周造 名古屋市立大学, 医薬学総合研究院(医学), 講師 (80551267)
• Project Period (FY): 2020-04-01 – 2024-03-31
• Project Status: Granted (Fiscal Year 2022)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2022: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2021: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2020: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 尿路結石 / マクロファージ / 貪食 / 結晶溶解 / オステオポンチン / 化合物ライブラリー / iPS細胞 / ノックアウトマウス

Outline of Research at the Start

[1] 培養Mφを用いたM1/M2マクロファージ結晶貪食解析法の確立： ① 培養Mφを用いたM1/M2誘導による貪食解析、② 既存薬ライブラリーによる貪食能の亢進薬の選出
[2] OPN欠損マウスを用いたマクロファージ分布解析・貪食解析：③ OPN添加f-COM結晶のMφ貪食試験、④ OPN欠損マウスの腎結晶・Mφを用いた貪食解析
[3] ヒトiPS細胞由来Mφを用いた腎結石貪食解析：⑤ ヒトiPS細胞由来MφのM1/M2への分化誘導とf-COM結晶貪食解析、⑥ ヒトiPS細胞由来Mφの腎結石の貪食解析と貪食促進薬の効果判定

Outline of Annual Research Achievements

[1] 培養Mφ・iPS細胞由来Mφによる結晶貪食モデルの確立
① 培養Mφを用いたM1/M2誘導による貪食解析：完了している。② 既存薬ライブラリーによる貪食能の亢進薬の選出：既存薬ライブラリーFDA-approved Drug Library (384-well)-L1300の約800種類の薬剤に関し貪食解析を完了した。現在追加で800種類の解析を行っている。一次スクリーニングn=3、二次スクリーニングn=10で行い、薬剤と結晶のみでの単独実験で直接作用を持つものを除外した。現在追加800種類の2次スクリーニングが終了しつつある。
[2] OPN欠損マウスを用いたマクロファージ分布解析・貪食解析
③ OPN添加f-COM結晶のMφ貪食試験：[1]①研究が完了していないため、着手していない。④ OPN欠損マウスの腎結晶・Mφを用いた貪食解析：OPNノックアウトマウスと野生型マウスから骨髄Mφ(Bone marrow-derived macrophage; BMDM)を採取し、COM結晶を暴露した。OPN-/-BMDMはOPNが有意に抑制されていたが、COM結晶の接着率は、OPN-/-型がOPN+/+型よりも有意に多かった。OPN-/-BMDMのIntgavとIntgb3の発現は、OPN+/+と比較してそれぞれ0.80倍(p=0.41), 1.61倍(p<1.61)であった。
[3] ヒトiPS細胞由来Mφを用いた腎結石貪食解析
⑤ ヒトiPS細胞由来MφのM1/M2への分化誘導とf-COM結晶貪食解析：結石患者・健常者由来iPS細胞MφからM1/M2に分化させることに成功した。また[1]の手法を用いてf-COM結晶を貪食することを確認できた。⑥ ヒトiPS細胞由来Mφの腎結石の貪食解析と貪食促進薬の効果判定：②の薬剤スクリーニング中であり、開始できていない。

Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

[1]IncuCyteによる正確な解析方法を確立し、既存薬ライブラリー研究を完了しつつある。
[2]またOPN-KOマウス研究も、骨髄由来Mφの結晶付着解析について確立し、発現解析も進みつつある。
[3]iPS細胞由来Mφの分化が成功し、結晶貪食を確認できた。

Strategy for Future Research Activity

[1]既存薬ライブラリーによる貪食能変化についての解析を継続する。
[2]引き続き　OPN欠損マウス骨髄由来マクロファージの貪食解析を進める。
[3] ヒトiPS細胞由来Mφを用いた腎結石貪食解析として、ヒトiPS細胞由来MφのM1/M2への分化誘導とf-COM結晶貪食解析ならび[1]で選出した薬剤の効果を確認する研究を行う。

Research Products

• [Journal Article] Clinical characteristics of Raoultella ornithinolytica bacteremia and antimicrobial susceptibility of Raoultella ornithinolytica (2023)
  • Author(s): Etani Toshiki、Kondo Shuhei、Yanase Takahiro、Morikawa Toshiharu、Aoki Maria、Gonda Masakazu、Tomiyama Nami、Nagai Takashi、Iida Keitaro、Iwatsuki Shoichiro、Taguchi Kazumi、Naiki Taku、Hamamoto Shuzo、Okada Atsushi、Kawai Noriyasu、Nakamura Atsushi、Yasui Takahiro
  • Journal Title: Journal of Infection and Chemotherapy Volume: 29 Issue: 5 Pages: 554-557
  • DOI: 10.1016/j.jiac.2023.01.023
  • Peer Reviewed
• [Journal Article] Combination therapy with radiation and hyperthermia‐induced clinical complete response of small cell carcinoma of prostate (2022)
  • Author(s): Kawai Noriyasu、Nagai Takashi、Naiki‐Ito Aya、Iida Keitaro、Etani Toshiki、Naiki Taku、Hamamoto Shuzo、Okada Atsushi、Murai Taro、Yasui Takahiro
  • Journal Title: IJU Case Reports Volume: 5 Issue: 2 Pages: 113-116
  • DOI: 10.1002/iju5.12413
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of fosfomycin in preventing infection after endoscopic combined intrarenal surgery in periods of limited supply of first‐ and second‐generation cephalosporins (2022)
  • Author(s): Etani Toshiki、Asaoka Minami、Kondo Shuhei、Wachino Chiharu、Tomiyama Nami、Hattori Tatsuya、Nagai Takashi、Iida Keitaro、Taguchi Kazumi、Naiki Taku、Hamamoto Shuzo、Okada Atsushi、Kawai Noriyasu、Yanagita Takeshi、Nakamura Atsushi、Yasui Takahiro
  • Journal Title: International Journal of Urology Volume: 29 Issue: 9 Pages: 977-982
  • DOI: 10.1111/iju.14896
  • Peer Reviewed
• [Journal Article] Long‐term survival of a patient with refractory advanced adrenocortical carcinoma after combination chemotherapy with paclitaxel and carboplatin plus mitotane (2022)
  • Author(s): Kobayakawa Yuki、Hamamoto Shuzo、Kamisawa Hideyuki、Okada Shinsuke、Taguchi Kazumi、Naiki Taku、Okada Atsushi、Tozawa Keiichi、Yasui Takahiro
  • Journal Title: IJU Case Reports Volume: 5 Issue: 4 Pages: 288-292
  • DOI: 10.1002/iju5.12458
  • Peer Reviewed / Open Access
• [Journal Article] A Randomized, Single-Blind Clinical Trial Comparing Robotic-Assisted Fluoroscopic-Guided with Ultrasound-Guided Renal Access for Percutaneous Nephrolithotomy (2022)
  • Author(s): Taguchi Kazumi、Hamamoto Shuzo、Okada Atsushi、Sugino Teruaki、Unno Rei、Kato Taiki、Fukuta Hidekatsu、Ando Ryosuke、Kawai Noriyasu、Tan Yung Khan、Yasui Takahiro
  • Journal Title: Journal of Urology Volume: 208 Issue: 3 Pages: 684-694
  • DOI: 10.1097/ju.0000000000002749
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Efficacy of ultrasound monitoring during extracorporeal shock wave lithotripsy: A multi‐institutional propensity score‐matched study (2022)
  • Author(s): Isogai Masahiko、Hamamoto Shuzo、Kawase Kengo、Okada Tomoki、Hattori Tatsuya、Chaya Ryosuke、Hamakawa Takashi、Sugino Teruaki、Taguchi Kazumi、Umemoto Yukihiro、Okada Atsushi、Yasui Takahiro
  • Journal Title: International Journal of Urology Volume: 29 Issue: 9 Pages: 1054-1060
  • DOI: 10.1111/iju.14984
  • Peer Reviewed
• [Journal Article] Microdamage analysis of single-use flexible ureteroscope immediately after lithotripsy use (2022)
  • Author(s): Sugino Teruaki、Taguchi Kazumi、Unno Rei、Hamamoto Shuzo、Ando Ryosuke、Okada Atsushi、Yasui Takahiro
  • Journal Title: Scientific Reports Volume: 12 Issue: 1 Pages: 18367-18367
  • DOI: 10.1038/s41598-022-23345-z
  • Peer Reviewed / Open Access
• [Journal Article] A Multicenter, Prospective, Non-randomized Study Evaluating Surgical Hand Preparation between Double-Gloving and Single-Gloving for Preventing Postoperative Infection in Robotic and Laparoscopic Minimally Invasive Surgeries (2022)
  • Author(s): Nagai Takashi, Taguchi Kazumi, Isobe Teruki, Matsuyama Nayuka, Hattori Tatsuya, Unno Rei, Kato Taiki, Etani Toshiki, Hamakawa Takashi, Fujii Yasuhiro, Ikegami Yosuke, Kamiya Hiroyuki, Hamamoto Shuzo, Nakane Akihiro, Ando Ryosuke, Maruyama Tetsuji, Okada Atsushi, Kawai Noriyasu, Yasui Takahiro
  • Journal Title: Urol J Volume: 20(2) Pages: 109-115
  • Peer Reviewed
• [Journal Article] Impact of pelvicalyceal anatomical variation on surgical outcomes of endoscopic combined intrarenal surgery (2022)
  • Author(s): Kawase Kengo、Hamamoto Shuzo、Taguchi Kazumi、Inoue Takaaki、Okada Shinsuke、Sugino Teruaki、Isogai Masahiko、Torii Koei、Yanase Takahiro、Okada Tomoki、Hattori Tatsuya、Chaya Ryosuke、Okada Atsushi、Yasui Takahiro、the SMART Study Group
  • Journal Title: BJUI Compass Volume: 4 Issue: 2 Pages: 173-180
  • DOI: 10.1002/bco2.209
  • Peer Reviewed / Open Access
• [Journal Article] Independent and interactive effects of kidney stone formation and conventional risk factors for chronic kidney disease: a follow-up study of Japanese men (2021)
  • Author(s): Ando Ryosuke、Nagaya Teruo、Suzuki Sadao、Takahashi Hidekatsu、Kawai Makoto、Taguchi Kazumi、Hamamoto Shuzo、Okada Atsushi、Yasui Takahiro
  • Journal Title: International Urology and Nephrology Volume: - Pages: 1081-1087
  • DOI: 10.1007/s11255-021-02803-z
  • Peer Reviewed / Open Access
• [Journal Article] Comparison of antegrade and retrograde ureterolithotripsy for proximal ureteral stones: a systematic review and meta-analysis (2021)
  • Author(s): Taguchi Kazumi、Hamamoto Shuzo、Osaga Satoshi、Sugino Teruaki、Unno Rei、Ando Ryosuke、Okada Atsushi、Yasui Takahiro
  • Journal Title: Translational Andrology and Urology Volume: 10 Issue: 3 Pages: 1179-1191
  • DOI: 10.21037/tau-20-1296
  • Peer Reviewed / Open Access
• [Journal Article] Taguchi KaRobot-assisted fluoroscopy-guided renal puncture for endoscopic combined intrarenal surgery: a pilot single-centre clinical trialzumi, Hamamoto Shuzo, Kato Taiki, Iwatsuki Shoichiro, Etani Toshiki, Okada Atsushi, Yasui Takahiro (2021)
  • Author(s): Taguchi K, Hamamoto S, Kato T, Iwatsuki S, Etani T, Okada A, Yasui T
  • Journal Title: BJU International Volume: 127 Issue: 3 Pages: 307-310
  • DOI: 10.1111/bju.15291
  • Peer Reviewed / Open Access
• [Journal Article] Macrophage Function in Calcium Oxalate Kidney Stone Formation: A Systematic Review of Literature (2021)
  • Author(s): Taguchi Kazumi、Okada Atsushi、Unno Rei、Hamamoto Shuzo、Yasui Takahiro
  • Journal Title: Frontiers in Immunology Volume: 12 Pages: 673690-673690
  • DOI: 10.3389/fimmu.2021.673690
  • Peer Reviewed / Open Access
• [Journal Article] Ureteroscopy‐assisted puncture for ultrasonography‐guided renal access significantly improves overall treatment outcomes in endoscopic combined intrarenal surgery (2021)
  • Author(s): Taguchi Kazumi、Yamashita Shimpei、Hamamoto Shuzo、Deguchi Ryusuke、Kawase Kengo、Okada Tomoki、Kato Taiki、Ando Ryosuke、Okada Atsushi、Kohjimoto Yasuo、Hara Isao、Yasui Takahiro
  • Journal Title: International Journal of Urology Volume: 28 Issue: 9 Pages: 913-919
  • DOI: 10.1111/iju.14603
  • Peer Reviewed
• [Journal Article] Comparison of the safety and efficacy between the prone split‐leg and Galdakao‐modified supine Valdivia positions during endoscopic combined intrarenal surgery: A multi‐institutional analysis (2021)
  • Author(s): Hamamoto Shuzo、Okada Shinsuke、Inoue Takaaki、Taguchi Kazumi、Kawase Kengo、Okada Tomoki、Chaya Ryosuke、Hattori Tatsuya、Okada Atsushi、Matsuda Tadashi、Yasui Takahiro、the SMART Study Group
  • Journal Title: International Journal of Urology Volume: 28 Issue: 11 Pages: 1129-1135
  • DOI: 10.1111/iju.14655
  • Peer Reviewed
• [Journal Article] Validation of the Japanese Version of The Wisconsin Stone Quality of Life Questionnaire: Results from SMART Study Group (2021)
  • Author(s): Okada Tomoki、Hamamoto Shuzo、Taguchi Kazumi、Okada Shinsuke、Inoue Takaaki、Fukuta Hidekatsu、Chew Ben H.、Penniston Kristina、Okada Atsushi、Yasui Takahiro
  • Journal Title: Journal of Endourology Volume: 35 Issue: 12 Pages: 1852-1856
  • DOI: 10.1089/end.2021.0292
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Bisphosphonate Use May Reduce the Risk of Urolithiasis in Astronauts on Long‐Term Spaceflights (2021)
  • Author(s): Okada Atsushi、Matsumoto Toshio、Ohshima Hiroshi、Isomura Tatsuya、Koga Tadashi、Yasui Takahiro、Kohri Kenjiro、LeBlanc Adrian、Spector Elisabeth、Jones Jeffrey、Shackelford Linda、Sibonga Jean
  • Journal Title: JBMR Plus Volume: 6 Issue: 1 Pages: 184-189
  • DOI: 10.1002/jbm4.10550
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A novel approach in creating nephrostomy using a double-lumen access sheath during endoscopic combined intrarenal surgery (2021)
  • Author(s): Unno Rei、Taguchi Kazumi、Hamamoto Shuzo、Hattori Tatsuya、Kawase Kengo、Okada Tomoki、Chaya Ryosuke、Tanaka Yutaro、Sugino Teruaki、Kato Taiki、Etani Toshiki、Ando Ryosuke、Okada Atsushi、Yasui Takahiro
  • Journal Title: Translational Andrology and Urology Volume: 10 Issue: 11 Pages: 4181-4191
  • DOI: 10.21037/tau-21-611
  • Peer Reviewed / Open Access
• [Journal Article] Surgical hand hygiene and febrile urinary tract infections in endourological surgery: a single-centre prospective cohort study (2020)
  • Author(s): Unno Rei, Taguchi Kazumi, Fujii Yasuhiro, Unno Naoko, Hamamoto Shuzo, Ando Ryosuke, Nakane Akihiro, Okada Atsushi, Kamiya Hiroyuki, Yasui Takahiro
  • Journal Title: Scientific Reports Volume: 10(1) Issue: 1 Pages: 14520-14520
  • DOI: 10.1038/s41598-020-71556-z
  • Peer Reviewed / Open Access
• [Journal Article] The First Case Report of Robot-Assisted Fluoroscopy-Guided Renal Access During Endoscopic Combined Intrarenal Surgery (2020)
  • Author(s): Taguchi Kazumi、Hamamoto Shuzo、Kawase Kengo、Hattori Tatsuya、Okada Tomoki、Chaya Ryosuke、Nagai Takashi、Kato Taiki、Okada Atsushi、Yasui Takahiro
  • Journal Title: Journal of Endourology Case Reports Volume: 6 Issue: 4 Pages: 310-314
  • DOI: 10.1089/cren.2020.0125
  • Peer Reviewed
• [Journal Article] Fatty acid?binding protein 4 downregulation drives calcification in the development of kidney stone disease (2020)
  • Author(s): Taguchi Kazumi、Chen Ling、Usawachintachit Manint、Hamamoto Shuzo、Kang Misun、Sugino Teruaki、Unno Rei、Tzou David T.、Sherer Benjamin A.、Okada Atsushi、Yasui Takahiro、Ho Sunita P.、Stoller Marshall L.、Chi Thomas
  • Journal Title: Kidney International Volume: - Issue: 5 Pages: 1042-1056
  • DOI: 10.1016/j.kint.2020.01.042
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Prospective evaluation and classification of endoscopic findings for ureteral calculi (2020)
  • Author(s): Hamamoto Shuzo, Okada Shinsuke, Inoue Takaaki, Sugino Teruaki, Unno Rei, Taguchi Kazumi, Ando Ryosuke, Okada Atsushi, Miura Hiroyasu, Matsuda Tadashi, Yasui Takahiro
  • Journal Title: SCIENTIFIC REPORTS Volume: 10 Issue: 1 Pages: 12292-12292
  • DOI: 10.1038/s41598-020-69158-w
  • Peer Reviewed
• [Journal Article] Dual ureteral stent placement after redo laser endoureterotomy to manage persistent ureteral stricture (2020)
  • Author(s): Isogai Masahiko、Hamamoto Shuzo、Hasebe Kenichi、Iida Keitaro、Taguchi Kazumi、Ando Ryosuke、Okada Atsushi、Yasui Takahiro
  • Journal Title: IJU Case Reports Volume: 3 Issue: 3 Pages: 93-95
  • DOI: 10.1002/iju5.12152
  • Peer Reviewed
• [Journal Article] 特集 尿路結石のすべて 【トピックス】Randall's plaque（結石原基） (2020)
  • Author(s): 田口 和己、濵本 周造、岡田 淳志、安井 孝周
  • Journal Title: 腎と透析 Volume: 88 Pages: 281-285
• [Journal Article] 〈尿路結石手術〉 体外衝撃波結石砕石術（ESWL）におけるトラブルシューティング (2020)
  • Author(s): 田口 和己、岡田 淳志、安井 孝周
  • Journal Title: 臨床泌尿器科 Volume: 74 Pages: 230-234
• [Journal Article] PNL＋TUL の標準化（特集：上部尿路結石治療の標準化～ Precision な結石治療のために） (2020)
  • Author(s): 濵本 周造、井上 貴昭、岡田 真介、河瀬 健吾、田口 和己、岡田 淳志、石戸 則孝、松崎 純一、奴田原 紀久雄、安井 孝周
  • Journal Title: Japanese Journal of Endourology Volume: 33 Pages: 66-70
• [Journal Article] PNLの標準化(特集３：上部尿路結石治療の標準化～Precisionな結石治療のために) (2020)
  • Author(s): 眞崎 拓朗、柿木 寛明、岡田 淳志、吉水 敦、山口 秋人
  • Journal Title: Japanese Journal of Endourology Volume: 33 Pages: 55-65
• [Journal Article] 肥満と尿路結石 (2020)
  • Author(s): 杉野 輝明、田中 勇太朗、海野 怜、田口 和己、濵本 周造、安藤 亮介、岡田 淳志、安井 孝周
  • Journal Title: 泌尿器科 Volume: 11 Pages: 606-612
  • NAID: 40022258505
• [Journal Article] 特集(症例から学ぶ 泌尿器疾患の見逃してはいけないサイン) 腰背部痛（膿尿あり） (2020)
  • Author(s): 岡田 淳志、田口 和己、濵本 周造、安藤 亮介、安井 孝周
  • Journal Title: 泌尿器Care&Cure Uro-Lo Volume: 25 Pages: 58-63
• [Journal Article] ECIRSを始める (2020)
  • Author(s): 濵本 周造、田口 和己、岡田 淳志、安井 孝周
  • Journal Title: 泌尿器外科 Volume: 33 Pages: 1297-1303
• [Presentation] Effectiveness of Virtual Reality simulation of renal puncture for endoscopic-combined intrarenal surgery (2022)
  • Author(s): Okada Atsushi, Ohashi Kazuya, Noda Yusuke, Okada Tomoki, Chaya Ryosuke, Kawase Kengo, Kato Taiki, Taguchi Kazumi, Hamamoto Shuzo, Tozawa Keiichi, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2022
  • Int'l Joint Research
• [Presentation] Discovery of drugs that promote the phagocytic ability of macrophages to calcium oxalate crystals using the FDA-approved Drug Library (2022)
  • Author(s): Okada Atsushi, Takase Hiroshi, Isogai Masahiko, Okada Tomoki, Hattori Tatsuya, Chaya Ryosuke, Kawase Kengo, Tanaka Yutaro, Kato Taiki, Sugino Teruaki, Unno Rei, Taguchi Kazumi, Hamamoto Shuzo, Ando Ryosuke, Hashita Tadahiro, Tozawa Keiichi, Matsunaga Tamihide, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2022
  • Int'l Joint Research
• [Presentation] A randomized, single-center, clinical trial comparing robotic-assisted fluoroscopic-guided with ultrasound-guided renal access for percutaneous nephrolithotomy (2022)
  • Author(s): Taguchi Kazumi, Hamamoto Shuzo, Yanase Takahiro, Sue Yasuhito, Hattori Tatsuya, Sugino Teruaki, Okada Atsushi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2022
  • Int'l Joint Research
• [Presentation] THE USEFULNESS OF ULTRASOUND MONITORING DURING EXTRACORPOREAL SHOCK WAVE LITHOTRIPSY (2021)
  • Author(s): Isogai Masahiko, Hamamoto Shuzo, Hattori Tatsuya, Chaya Ryosuke, Okada Tomoki, Kawase Kengo, Kato Taiki, Taguchi Kazumi, Kobayashi Takahiro, Okada Atsushi, Umemoto Yukihiro, Tozawa Keiichi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2021
  • Int'l Joint Research
• [Presentation] STONE FORMATION BY ANALYSIS OF UROFLOW DYNAMICS IN THE RENAL PELVIS (2021)
  • Author(s): Kawase Kengo,Hamamoto Shuzo, Hattori Shota, chaya Ryosuke, Okada Tomoki, Tanaka Yutaro, Sugino Takeru, Unno Rei, Taguchi Kazumi, Ando Ryosuke, Okada Atsushi, Nakamura Masanori, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2021
  • Int'l Joint Research
• [Presentation] THE MECHANISM OF KIDNEY STONE SUPPRESSION BY PROLINE HYDROXYLASE INHIBITOR ADMINISTRATION (2021)
  • Author(s): Kawase Kengo, Hamamoto Shuzo, Hattori Tatsuya, Okada Tomoki, Tanaka Yutaro, Sugino Takeru, Unno Rei, Taguchi Kazumi, Ando Ryosuke, Okada Atsushi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2021
  • Int'l Joint Research
• [Presentation] FATTY ACID BINDING PROTEIN 4 ATTENUATES MACROPHAGE AND TUBULAR CELLS CRYSTAL PHAGOCYTOSIS TO DRIVE RENAL CALCIUM OXALATE STONE DEVELOPMENT (2021)
  • Author(s): Taguchi Kazumi, Unno Rei, Sugino Teruaki, Kawase Kengo, Heiko Yang, Hamamoto Shuzo, Okada Atsushi, Marshall Stoller, Yasui Takahiro, Tom Chi,
  • Organizer: American Urological Association Annual Meeting 2021
  • Int'l Joint Research
• [Presentation] COMPARISON OF SAFETY AND EFFICACY BETWEEN PRONE SPLIT-LEG AND MODIFIED SUPINE VALDIVIA POSITIONS IN ENDOSCOPIC COMBINED INTRARENAL SURGERY (ECIRS): A MULTI-INSTITUTIONAL ANALYSIS (2021)
  • Author(s): Hamamoto Shuzo, Okada Shinsuke, Inoue Takaaki, Taguchi Kazumi, Kawase Kengo, Okada Tomoki, Chaya Ryosuke, Hattori Tatsuya, Okada Atsushi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2021
  • Int'l Joint Research
• [Presentation] URETEROSCOPY-ASSISTED VERSUS CONVENTIONAL ULTRASOUND-GUIDED RENAL ACCESS FOR MINIATURIZED ENDOSCOPIC COMBINED INTRARENAL SURGERY: A MULTICENTER COMPARATIVE STUDY (2021)
  • Author(s): Taguchi Kazumi, Yamashita Shimpei, Hamamoto Shuzo, Deguchi Ryusuke, Kawase Kengo, Okada Tomoki, Unno Rei, Kato Taiki, Ando Ryosuke, Okada Atsushi,Kohjimoto Yasuo, Hara Isao, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2021
  • Int'l Joint Research
• [Presentation] BROWN ADIPOCYTES COULD PREVENT URINARY STONES IN THE HUMAN BODY (2021)
  • Author(s): Sugino Teruaki, Taguchi Kazumi, Unno Rei, Hamamoto Shuzo, Ando Ryosuke, Okada Atsushi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2021
  • Int'l Joint Research
• [Presentation] ROBOTIC-ASSISTED FLUOROSCOPIC-GUIDED RENAL PUNCTURE HAS COMPARABLE SURGICAL OUTCOMES WITH ULTRASOUND-GUIDED IN ENDOSCOPIC COMBINED INTRARENAL SURGERY: A RANDOMIZED CONTROLLED TRIAL (2021)
  • Author(s): Taguchi Kazumi, Hamamoto Shuzo, Sue Yasuhito, Kobayakawa Yuki, Hattori Tatsuya, Okada Tomoki, Chaya Ryosuke, Sugino Teruaki, Kato Taiki, Okada Atsushi, Yasui Takahiro
  • Organizer: Taguchi Kazumi, Hamamoto Shuzo, Sue Yasuhito, Kobayakawa Yuki, Hattori Tatsuya, Okada Tomoki, Chaya Ryosuke, Sugino Teruaki, Kato Taiki, Okada Atsushi, Yasui Takahiro
  • Int'l Joint Research
• [Presentation] Basic research toward dissolution therapy for calcium oxalate stones (2021)
  • Author(s): Okada Atsushi, Okada Tomoki, Sugino Teruaki, Unno Rei, Taguchi Kazumi, Hamamoto Shuzo, Ando Ryosuke, Tozawa Keiichi, Kohri Kenjiro, Yasui Takahiro
  • Organizer: THE 37th KOREA-JAPAN UROLOGICAL CONGRESS
  • Int'l Joint Research / Invited
• [Presentation] The impact protein mapping in human kidney stone by multiple multifaceted structure analysis (2021)
  • Author(s): Tanaka Yutaro, Okada Atsushi, Maruyama Mihoko, Tajiri Rie, Sugino Teruaki, Taguchi Kazumi, Hamamoto Shuzo, Mori Yusuke, Kohri Kenjiro, Yasui Takahiro
  • Organizer: THE 37th KOREA-JAPAN UROLOGICAL CONGRESS
  • Int'l Joint Research
• [Presentation] M2 macrophages differentiated from iPS cells have higher abilities to phagocytose and process calcium oxalate crystals than similarly induced M1 macrophages (2020)
  • Author(s): Okada Atsushi, Aoki Hiromasa, Onozato Daichi, Kato Taiki, Takase Hiroshi, Ohshima Shigeru, Banno Rika, Taguchi Kazumi, Hashita Tadahiro, Matsunaga Tamihide, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Usefulness of determination of renal puncture line in ECIRS using Virtual Reality (2020)
  • Author(s): Okada Atsushi , Noda Yusuke, Ohashi Kazuya, Okada Tomoki, Chaya Ryosuke, Kawase Kengo, Kato Taiki, Taguchi Kazumi, Hamamoto Shuzo, Hirose Yasujiro, Tozawa Keiichi, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] The impact of SWL hands-on training on maintaining high success rate and identification of performance improvement factors (2020)
  • Author(s): Okada Atsushi, Tanaka Yutaro, Sugino Teruaki, Unno Rei, Banno Rika, Hamamoto Shuzo, Ando Ryosuke, Tozawa Keiichi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Impact of insulin resistance and physical activity on kidney stone formation in the Japanese population: A prospective cohort study (2020)
  • Author(s): Ando Ryosuke, Suzuki Sadao, Nishiyama Takeshi, Nakagawa Hiroko,Hosono Akihiro, Watanabe Miki, Yamada Tamaki, Taguchi Kazumi, Hamamoto Shuzo, Okada Atsushi, Tozawa Keiichi, Kohri Kenjiro,Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] The importance of hands-on training for flexible ureteroscope (fURS): Appropriate training as examined based on questionnaires (2020)
  • Author(s): Hamamoto Shuzo, Okada Shinsuke, Inoue Takaaki, Okada Tomoki, Chaya Ryosuke, Kawase Kengo, Taguchi Kazumi, Okada Atsushi, Miura Hiroyasu, Matsuda Tadashi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Prospective evaluation of endoscopic findings and analyses of the risk factors for ureteral changes during URSL (2020)
  • Author(s): Hamamoto Shuzo, Okada Shinsuke, Inoue Takaaki, Okada Tomoki, Chaya Ryosuke, Kawase Kengo, Taguchi Kazumi, Okada Atsushi, Miura Hiroyasu, Matsuda Tadashi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Investigation of the ideal position for endoscopic combined intrarenal surgery: Prone split-leg position vs. modified Valdivia position (2020)
  • Author(s): Hamamoto Shuzo, Okada Shinsuke, Inoue Takaaki, Okada Tomoki, Chaya Ryosuke, Kawase Kengo, Taguchi Kazumi, Okada Atsushi, Miura Hiroyasu, Matsuda Tadashi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Impact of preoperative ureteral stenting on endoscopic findings: A propensity score matching analysis (2020)
  • Author(s): Hamamoto Shuzo, Okada Shinsuke, Inoue Takaaki, Okada Tomoki, Chaya Ryosuke, Kawase Kengo, Taguchi Kazumi, Okada Atsushi, Miura Hiroyasu, Matsuda Tadashi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Fatty acid binding protein 4 drives calcification in the development of kidney stone disease (2020)
  • Author(s): Taguchi Kazumi, Chen Ling, Hamamoto Shuzo, Sugino Teruaki, Kang Misun, Unno Rei, Benjamin A Sherer, Manint Usawachintachit, David T Tzou, Okada Atsushi, Sunita P Ho, Marshall L Stoller, Yasui Takahiro , and Thomas Chi
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Robotic-assisted fluoroscopic guidance provides more accurate and confident renal access than ultrasound guidance (2020)
  • Author(s): Taguchi Kazumi, Okada Tomoki, Kawase Kengo, Sugino Teruaki, Tanaka Yutaro, Unno Rei, Kato Taiki, Hamamoto Shuzo, Ando Ryosuke, Okada Atsushi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Administration of antioxidants inhibits the development of renal crystals via Keap1/Nrf2 pathway (2020)
  • Author(s): Unno Rei, Kawase Kengo, Sugino Teruaki, Tanaka Yutaro, Taguchi Kazumi, Unno Naoko, Hamamoto Shuzo, Ando Ryosuke, Okada Atsushi, Nakane Akihiro, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Surgical hand hygiene and febrile urinary tract infections in endourological surgery: a single-center prospective cohort study (2020)
  • Author(s): Unno Rei, Taguchi Kazumi, Fuji Yasuhiro, Unno Naoko, Hamamoto Shuzo, Ando Ryosuke, Okada Atsushi, Nakane Akihiro, Kamiya Hiroyuki, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Brown adipocytes suppress kidney stone formation via heat-producing protein, uncoupling protein 1 (2020)
  • Author(s): Sugino Teruaki, Okada Atsushi, Chaya Ryosuke, Tanaka Yutaro, Unno Rei, Taguchi Kazumi, Hamamoto Shuzo, Ando Ryosuke, Mogami Tohru, Yamashita Hitoshi, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Double ureteral stent placement reduces restenosis after incision and dilatation for ureteral stenosis (2020)
  • Author(s): Sugino Teruaki, Hamamoto Shuzo, Isogai Masahiko, Tanaka Yutaro, Unno Rei, Taguchi Kazumi, Ando Ryosuke, Okada Atsushi, Mogami Tohru, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] Discovering spatial distribution and differential localization of protein matrix in calcium oxalate stones; A novel method of multifaceted structure analysis (2020)
  • Author(s): Tanaka Yutaro, Okada Atsushi, Maruyama Mihoko, Tagiri Rie, Sugino Teruaki, Unno Rei, Taguchi Kazumi, Hamamoto Shuzo, Ando Ryosuke, Yoshimura Masashi, Mori Yusuke, Kohri Kenjiro, Yasui Takahiro
  • Organizer: American Urological Association Annual Meeting 2020
  • Int'l Joint Research
• [Presentation] 尿路感染症を契機に発見された小児シスチン結石の1例 (2020)
  • Author(s): 岡田 朋記、田口 和己、松本 大輔、河瀬 健吾、加藤 大貴、西尾 英紀、濵本 周造、水野 健太郎、岡田 淳志、戸澤 啓一、林 祐太郎、安井 孝周
  • Organizer: 第284回日本泌尿器科学会東海地方会
• [Presentation] 原発性副甲状腺機能亢進症における尿路結石リスク因子におよぼす副甲状腺摘除術の効果 (2020)
  • Author(s): 岡田 淳志、茶谷 亮輔、岡田 朋記、河瀬 健吾、田中 勇太朗、杉野 輝明、海野 怜、田口 和己、濵本 周造、安藤 亮介、戸澤 啓一、郡 健二郎、安井 孝周
  • Organizer: 第93回日本内分泌学会学術総会
• [Presentation] 尿路結石の発症にインスリン抵抗性がおよぼす影響 (2020)
  • Author(s): 安藤 亮介、鈴木 貞夫、西山 毅、中川 弘子、細野 晃弘、渡邉 美貴、田口 和己、濵本 周造、岡田 淳志、山田 珠樹、郡 健二郎、安井 孝周
  • Organizer: 第93回日本内分泌学会学術総会
• [Presentation] 骨密度が影響を及ぼす尿路結石症患者の臨床アウトカムの横断的および縦断的関連解析 (2020)
  • Author(s): 田口 和己、濵本 周造、岡田 淳志、河瀬 健吾、岡田 朋記、茶谷 亮輔、加藤 大貴、広瀬 真仁、遠藤 純夫、坂倉 毅、安藤 亮介、戸澤 啓一、郡 健二郎、安井 孝周
  • Organizer: 第93回日本内分泌学会学術総会
• [Presentation] 隕石学的技術を応用した二次元マッピング法による腎結石の構造解析 (2020)
  • Author(s): 田中 勇太朗、岡田 淳志、丸山 美帆子、田口 和己、濵本 周造、森 勇介、郡 健二郎、安井 孝周
  • Organizer: 第63回日本腎臓学会学術総会
• [Presentation] PHD 阻害剤の投与による腎結石形成抑制効果の検討 (2020)
  • Author(s): 河瀬 健吾、濱本 周造、服部 竜也、茶谷 亮輔、岡田 朋記、田中 勇太朗、杉野 輝明、海野 怜、田口 和己、安藤 亮介、岡田 淳志、安井 孝周
  • Organizer: 第63回日本腎臓学会学術総会
• [Presentation] 埋没型腎癌に対するRAPNにおけるResection Process Mapの有用性 (2020)
  • Author(s): 岡田 淳志
  • Organizer: The 11th Tokai Robotic Urology Symposium
• [Presentation] ECIRSにおける感染性合併症と対策 (2020)
  • Author(s): 惠谷 俊紀、濵本 周造、田口 和己、岡田 淳志、内木 拓、河合 憲康、安井 孝周
  • Organizer: 第70回日本泌尿器科学会中部総会
• [Presentation] 尿路結石症の未来へ～基礎研究から予防を再考する～ (2020)
  • Author(s): 杉野 輝明、田中 勇太朗、海野 怜、田口 和己、濵本 周造、安藤 亮介、岡田 淳志、安井 孝周
  • Organizer: 第70回日本泌尿器科学会中部総会
• [Presentation] 尿路結石に対するESWLの基本手技 (2020)
  • Author(s): 岡田 淳志
  • Organizer: 第70回日本泌尿器科学会中部総会
  • Invited
• [Presentation] 尿管狭窄の内視鏡治療において狭窄の距離と成因が治療成績に影響を与える (2020)
  • Author(s): 杉野 輝明、田口 和己、濵本 周造、岡田 淳志、磯谷 正彦、田中 勇太朗、海野 怜、安藤 亮介、岡田 淳志、安井 孝周
  • Organizer: 第70回日本泌尿器科学会中部総会
• [Presentation] PHD阻害剤の投与による腎結石形成抑制効果の検討 (2020)
  • Author(s): 河瀬 健吾、濵本 周造、服部 竜也、茶谷 亮輔、岡田 朋記、田中 勇太朗、杉野 輝明、海野 怜、田口 和己、岡田 淳志、戸澤 啓一、安井 孝周
  • Organizer: 第70回日本泌尿器科学会中部総会
• [Presentation] ESWL砕石率UPのための心得 (2020)
  • Author(s): 岡田 淳志、海野 怜、加藤 大貴、田口 和己、濵本 周造、安藤 亮介、戸澤 啓一、郡 健二郎、安井 孝周
  • Organizer: 第34回日本泌尿器内視鏡学会総会
  • Invited
• [Presentation] 浸潤性膀胱癌に対して、RARC および体腔内回腸導管造設を行った初期症例の検討 (2020)
  • Author(s): 磯部 輝紀、岡田 淳志、濵本 周造、岩月 正一郎、惠谷 俊紀、田口 和己、内木 拓、河合 憲康、戸澤 啓一、安井 孝周
  • Organizer: 第34回日本泌尿器内視鏡学会総会
• [Presentation] 没型腎腫瘍に対するRAPN におけるResectionProcess Map の有用性 (2020)
  • Author(s): 岡田 淳志、田口 和己、惠谷 俊紀、岩月 正一郎、濵本 周造、内木 拓、安藤 亮介、河合 憲康、戸澤 啓一、安井 孝周
  • Organizer: 第34回日本泌尿器内視鏡学会総会
• [Presentation] ロボット支援下前立腺全摘除術における術後尿禁制に対する膜様部尿道温存術式の検討 (2020)
  • Author(s): 中根 明宏、海野 怜、神沢 英幸、廣瀬 泰彦、岡田 淳志、窪田 裕樹、戸澤 啓一、安井 孝周
  • Organizer: 第34回日本泌尿器内視鏡学会総会
• [Presentation] 尿路結石形成に関わる遺伝子ネットワークの同定と溶解治療への可能性 (2020)
  • Author(s): 田口 和己、濵本 周造、岡田 淳志、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
  • Invited
• [Presentation] 尿管内視鏡所見から考える経尿道的尿管砕石術における適切な治療介入時期の解析 (2020)
  • Author(s): 濵本 周造、岡田 真介、井上 貴昭、田口 和己、安藤 亮介、岡田 淳志、三浦 浩康、松田 公志、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] ESWL エキスパートによる砕石率を高めるためのルーティン (2020)
  • Author(s): 岡田 淳志
  • Organizer: 第108回日本泌尿器科学会総会
  • Invited
• [Presentation] mTOR/TFEB シグナル障害によるオートファジーの低下が腎結石形成の誘因となる (2020)
  • Author(s): 海野 怜、川端 剛、杉野 輝明、田口 和己、濵本 周造、安藤 亮介、岡田 淳志、郡 健二郎、吉森 保、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] 馬蹄腎に発生した尿路結石への内視鏡治療の検討 (2020)
  • Author(s): 河瀨 健吾、濵本 周造、茶谷 亮輔、岡田 朋記、田中 勇太朗、杉野 輝明、海野 怜、田口 和己、宇佐美 雅之、安藤 亮介、岡田 淳志、戸澤 啓一、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] 15 歳以下でみつかった小児上部尿路結石の再発率と結石形成の原因 (2020)
  • Author(s): 加藤 大貴、田口 和己、濵本 周造、田中 勇太朗、杉野 輝明、海野 怜、西尾 英紀、安藤 亮介、岡田 淳志、水野 健太郎、戸澤 啓一、林 祐太郎、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] 当院でのECIRS 手術症例および合併症の検討 (2020)
  • Author(s): 宇佐美 雅之、金本 一洋、橋本 良博、岩瀬 豊、山田 健司、海野 奈央子、濵本 周造、岡田 淳志、戸澤 啓一、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] 経尿道的尿管砕石術後の術後合併症に対する臨床的検討 (2020)
  • Author(s): 茶谷 亮輔、濵本 周造、岡田 朋記、河瀨健吾、田口 和己、安藤 亮介、岡田 淳志、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] 尿路結石の発症にインスリン抵抗性がおよぼす影響 (2020)
  • Author(s): 安藤 亮介、鈴木 貞夫、西山 毅、中川 弘子、細野 晃弘、渡邉 美貴、田口 和己、濵本 周造、岡田 淳志、戸澤 啓一、山田 珠樹、郡 健二郎、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] mTOR 活性異常が腎結石形成の誘因となる (2020)
  • Author(s): 海野 怜、河瀬 健吾、海野 奈央子、田中勇太郎、杉野 輝明、田口 和己、浜本 周造、安藤 亮介、中根 明宏、岡田 淳志、坂倉 毅、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] 隕石学・鉱物学的技術を応用した二次元マッピング法による尿路結石の多面的構造解析の検討 (2020)
  • Author(s): 田中 勇太朗、岡田 淳志、丸山 美帆子、田尻 理恵、杉野 輝明、海野 怜、田口 和己、濵本 周造、安藤 亮介、吉村 政志、森 勇介、郡 健二郎、安井孝周
  • Organizer: 第108回日本泌尿器科学会総会
• [Presentation] 褐色脂肪細胞の熱産生タンパクUCP1 による腎結石制御メカニズムの解明 (2020)
  • Author(s): 杉野 輝明、田中 勇太朗、海野 怜、田口和己、濵本 周造、遠藤 純央、安藤 亮介、岡田 淳志、戸澤 啓一、最上 徹、郡 健二郎、山下 均、安井 孝周
  • Organizer: 第108回日本泌尿器科学会総会