Periodontal tissue regeneration using a complex cell sheet
| Project/Area Number |
20K23057
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
0907:Oral science and related fields
|
|---|
| Research Institution | National Center for Geriatrics and Gerontology (2021)
The University of Tokushima (2020) |
|---|
Principal Investigator |
ラジュ レスミ 国立研究開発法人国立長寿医療研究センター, ジェロサイエンス研究センター, 研究員 (40876702)
|
|---|
| Project Period (FY) |
2020-09-11 – 2022-03-31
|
| Project Status |
Completed (Fiscal Year 2021)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2021: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2020: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | regeneration / tissue engineering / periodontal tissue / tooth regeneration / Periodontal tissue / Regenerative medicine / Tissue engineering |
|---|
| Outline of Research at the Start |
Periodontal tissue is composed of cementum, periodontal ligament (PDL) and alveolar bone, given the complexity of this redundant structure, a single type of cell is not enough to regenerate such combined tissue. Here we are developing a complex cell sheet composed of PDL and osteogenic differentiated PDL cells. Furthermore, we will be analyzing the regenerative capacity of this cell sheet by ectopic and orthotopic transplantation in mice.
|
| Outline of Annual Research Achievements |
Complex cell sheet, CCS was made using rat PDL cells and osteogenic differentiated PDL cells. Transplants from ectopic transplantation analysis using histology and micro-CT showed the formation of periodontal ligament like bone like tissue. Furthermore, we have successfully created an orthotropic injury mice model for the transplantation of cell sheets. In a continuation study, we have evaluated the regenerative capacity of tooth dentin-pulp tissue by a CCS. Dental pulp stem cells, DPSCs and odontogenic differentiated DPSCs were utilized to fabricate a CCS. Ectopic transplants after 8 weeks of transplantation revealed the formation of dentin-pulp like combined hard and soft tissue formation.
|
Report
(2 results)
Research Products
(2 results)
