| Budget Amount *help |
¥30,940,000 (Direct Cost: ¥23,800,000、Indirect Cost: ¥7,140,000)
Fiscal Year 2011: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2010: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
Fiscal Year 2009: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
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| Research Abstract |
To recover the CHO chromosome that carry 3.5kb of the lacO repeat sequences in the cells, we carried out immunoprecipitation by using anti-GFP antibody after the introduction of the LacI-GFP vector. As a result, a large size of CHO chromosome was recovered by anti-GFP antibody. These results suggest that the possibility of recovery of the HAC by chromatin immunoprecipitation. In addition, our colleague has developed a new HAC vector (MI-HAC) with multi-integrase recombination sites. MI-HAC enables to introduce various large genes or genomic loci. Furthermore, we produced MI-HAC that contains the lacO repeat sequences and targeted gene loci (Xist, LIT1/KCNQ1OT1 and CGG repeat sequence).
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