Analysis of Cell Invasion System and In Vivo Stealth Activityof Bio-nanocapsules

• Project/Area Number: 21240052
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Nagoya University
• Principal Investigator: KURODA Shunichi 名古屋大学, 大学院・生命農学研究科, 教授 (60263406)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥47,320,000 (Direct Cost: ¥36,400,000、Indirect Cost: ¥10,920,000); Fiscal Year 2011: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000); Fiscal Year 2010: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000); Fiscal Year 2009: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
• Keywords: ナノメディシン / DDS / ナノキャリア / ウイルス / ナノバイオ / バイオテクノロジー / マイクロ・ナノデバイス / 遺伝子治療

Research Abstract

We previously developed a hepatitis B virus (HBV) surface antigen L protein particle [bio-nanocapsule (BNC)] as a nonviral DDS nanocarrier. Intravenously injected BNCs were shown to avoid clearance by RES (reticuloendothelial system)-rich organs and accumulate in target tissues. In this study, since the surface modification with albumins is known to prolong the circulating half-life of nanomedicines, we demonstrated that the polymerized albumin receptor (PAR) of BNCs contributes to RES evasion in mouse liver,strongly suggesting that BNCs, as well as HBV, harbor an innate RES evasion mechanism. Therefore, the surface modification with PAR peptides could be an alternative strategy forimproving pharmacodynamics and pharmacokinetics of forthcoming nanomedicines.

Research Products

• [Journal Article] An automated system for high-throughput single cell-based breeding (2013)
  • Author(s): N Yoshimoto, A Kida, X Jie, M Kurokawa, M Iijima, T Niimi, A D. Maturana, I Nikaido, H R. Ueda, K Tatematsu, K Tanizawa, A Kondo, I Fujii & S Kuroda
  • Journal Title: Sci. Rep. Volume: 3 Pages: 1191-1191
  • Peer Reviewed
• [Journal Article] Cell surface-fluorescence immunosorbent assay for real-time detection of hybridomas with efficient antibody secretion at the single-cell level (2013)
  • Author(s): Akiko Kida, Masumi Iijima, Tomoaki Niimi, Andrés D. Maturana, Nobuo Yoshimoto*, and Shun’ichi Kuroda
  • Journal Title: Anal Chem Volume: 1753-1759 Pages: 85-85
  • Peer Reviewed
• [Journal Article] Engineered bio-nanocapsules, hepatitis B virus surface antigen L protein particles, for in vivo active targeting to splenic dendritic cells (2012)
  • Author(s): Matsuo, M., Yoshimoto, N., Iijima, M., Niimi, T., Jung, J., Jeong, S. Y., Choi, E. K., Sewaki, T., Arakawa, T., and Kuroda, S
  • Journal Title: Int. J. Nanomed. Volume: 7 Pages: 3341-3350
  • Peer Reviewed
• [Journal Article] Targeting of Polyplex to Human Hepatic Cells by Bio-nanocapsules, Hepatitis B Virus Surface Antigen L Protein Particles (2012)
  • Author(s): Somiya, M., Yoshimoto, N., Iijima, M., Niimi, T., Dewa, T., Jung, J., and Kuroda, S
  • Journal Title: Bioorg. Med. Chem. Volume: 20 Pages: 3872-3879
  • Peer Reviewed
• [Journal Article] Hepatitis B Virus Envelope L Protein-Derived Bio-Nanocapsules: Mechanisms of Cellular Attachment and Entry into Human Hepatic Cells (2012)
  • Author(s): Yamada, M., Oeda A., Jung J.H., Iijima M., Yoshimoto, N., Niimi, T., Jeong, S.Y., Choi, E.K., Tanizawa, K., and Kuroda, S
  • Journal Title: J. Controlled Release Volume: 160 Pages: 322-329
  • Peer Reviewed
• [Journal Article] Electroporation and use of hepatitis B virus envelope L proteins asbionanocapsules (2012)
  • Author(s): Yamada T, Jung J, Seno M, Kondo A, Ueda M, Tanizawa K, and Kuroda S
  • Journal Title: Cold Spring Harb Protoc Volume: 6 Pages: 702-705
  • Peer Reviewed
• [Journal Article] バイオナノカプセル(ウイルス感染機構を備えたナノキャリア)を用いる生体内ピンポイントDDSの構築 (2012)
  • Author(s): 黒田 俊一
  • Journal Title: 高分子 Volume: Vol. 61. No. 8 Pages: 530-532
  • NAID: 10030931960
  • Peer Reviewed
• [Journal Article] Hepatitis B Virus Envelope L Protein-Derived Bio-Nanocapsules: Mechanisms of Cellular Attachment and Entry into Human Hepatic Cells. (2012)
  • Author(s): Yamada, M., Oeda A., Jung J.H., Iijima M., Yoshimoto, N., Niimi, T., Jeong, S.Y., Choi, E.K., Tanizawa, K., and Kuroda, S.
  • Journal Title: J. Controlled Release Volume: 160 Pages: 322-329
  • Peer Reviewed
• [Journal Article] Targeting of Polyplex to Human Hepatic Cells by Bio-nanocapsules, Hepatitis B Virus Surface Antigen L Protein Particles (2012)
  • Author(s): Somiya, M., Yoshimoto, N., Iijima, M., Niimi, T., Dewa, T., Jung, J., and Kuroda, S.
  • Journal Title: Bioorg. Med. Chem. Volume: 20 Pages: 3873-3879
  • Peer Reviewed
• [Journal Article] Nano-visualization of oriented-immobilized IgGs on immunosensors by high-speed atomic force microscopy (2012)
  • Author(s): Iijima, M., Somiya, M., Yoshimoto, N., Niimi, T., and Kuroda, S.
  • Journal Title: Sci. Rep. Volume: 2 Pages: 790-790
  • Peer Reviewed
• [Journal Article] バイオナノカプセル（ウイルス感染機構を備えたナノキャリア）を用いる生体内ピンポイントＤＤＳの構築 (2012)
  • Author(s): 黒田俊一
  • Journal Title: 高分子 Volume: 61 Pages: 530-532
  • NAID: 10030931960
  • Peer Reviewed
• [Journal Article] Electroporation and use of hepatitis B virus envelope L proteins as bionanocapsules. (2012)
  • Author(s): Yamada T, Jung J, Seno M, Kondo A, Ueda M, Tanizawa K, Kuroda S
  • Journal Title: Cold Spring Harb Protoc. Volume: 6 Pages: 702-705
  • Peer Reviewed
• [Journal Article] 高度な肝臓細胞標的化能及び感染能を有するバイオナノカプセル－リポプレックス複合体の開発 (2012)
  • Author(s): 宮部康平，太江田綾子，山田光男，黒田俊一
  • Journal Title: DDS研究の進歩 （奥直人、山田静雄、賀川義之、板井茂、並木徳之 編集） Volume: 20 Pages: 97-102
• [Journal Article] Fluorophore-labeled nanocapsules displaying IgG Fc-binding domains for the simultaneous detection of multiple antigens. (2011)
  • Author(s): Iijima M, Matsuzaki T, Yoshimoto N, Niimi T, Tanizawa K, and Kuroda S.
  • Journal Title: Biomaterials Volume: 32 Pages: 9011-9020
  • Peer Reviewed
• [Journal Article] Efficient and rapid purification of drugand gene-carrying bio-nanocapsules, hepatitis B virus surface antigen L particles, from Saccharomyces cerevisiae (2011)
  • Author(s): Jung J., Iijima M., Yoshimoto N., Sasaki M., Niimi T., Tatematsu K., Jeong S.Y., Choi E.K., Tanizawa K., and Kuroda S
  • Journal Title: Protein Exp. Purif Volume: 78 Pages: 149-155
  • Peer Reviewed
• [Journal Article] Human Liver Specific Nano-Carrier in a Novel Mouse Xenograft Model Bearing Non-Cancerous Human Liver Tissue (2011)
  • Author(s): Matsuura Y, Yagi H, Matsuda S, Itano O, Koichi A, Kuroda S, Ueda M, and Kitagawa Y
  • Journal Title: European Surgical Research Volume: 46 Pages: 65-72
  • Peer Reviewed
• [Journal Article] Nanocapsules Incorporating IgG Fc-binding Domain Derived from Staphylococcus aureus Protein-A for Displaying IgGs on Immunosensor Chips (2011)
  • Author(s): Iijima M, Kadoya H, Matsuzaki T, Hatahira S, Hiramatsu S, Jung G, Martin A, Quinn J, Jung J, Jeong SY, Choi EK, Arakawa T, Hinako F, Kusunoki M, Yoshimoto N, Niimi T, Tanizawa K, and Kuroda S
  • Journal Title: Biomaterials Volume: 32 Pages: 1455-1464
  • Peer Reviewed
• [Journal Article] Efficient and rapid purification of drug- and gene-carrying bio-nanocapsules, hepatitis B virus surface antigen L particles, from Saccharomyces cerevisiae (2011)
  • Author(s): Jung J., Iijima M., Yoshimoto N., Sasaki M., Niimi T., Tatematsu K., Jeong S.Y., Choi E.K., Tanizawa K., and Kuroda S.
  • Journal Title: Protein Exp. Purif. Volume: 23 Pages: 928-934
  • Peer Reviewed
• [Journal Article] Bio-nanocapsules for Oriented Immobilization of IgGs on Immunosensor Chips (2011)
  • Author(s): Masumi Iijima and Shun'ichi Kuroda
  • Journal Title: 5th European Conference of the International Federation for Medical and Biological Engineering （Akos Jobbagy Ed.） IFMBE Proceedings Volume: 37 Pages: 1000-1003
  • Peer Reviewed
• [Journal Article] A gene delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer (2010)
  • Author(s): Oishi J, Jung J, Tsuchiya A, Toita R, Kang JH, Mori T, Niidome T, Tanizawa K, Kuroda S, and Katayama Y
  • Journal Title: Int J Pharm Volume: 396 Pages: 174-178
  • Peer Reviewed
• [Journal Article] Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule (2010)
  • Author(s): Kang JH, Oishi J, Kim JH, Ijuin M, Toita R, Jun B, Asai D, Mori T, Niidome T, Tanizawa K, Kuroda S, and Katayama Y
  • Journal Title: Nanomedicine Volume: 6 Pages: 583-589
  • Peer Reviewed
• [Journal Article] Bio-nanocapsule-based enzyme-antibody conjugates for ELISA (2010)
  • Author(s): Iijima M, Matsuzaki T, Kadoya H, Hatahira S, Hiramatsu S, Jung G, Tanizawa K, and Kuroda S
  • Journal Title: Anal Biochem Volume: 396 Pages: 257-261
  • Peer Reviewed
• [Journal Article] Splice variants of Enigma Homolog, differentially expressed during heart development, promote or prevent hypertrophy. (2010)
  • Author(s): Yamazaki T., Waelchi S., Fujita T., Ryser S., Hoshijima M., Schlegel W., Kuroda S., Maturana A.D.
  • Journal Title: Cardiovascular Research Volume: 86 Pages: 374-382
  • Peer Reviewed
• [Journal Article] Hepatoma-targeted gene delivery using a tumor cell-specific gene regulation system combined with a human liver cell-specific bionanocapsule. (2010)
  • Author(s): Kang JH, Oishi J, Kim JH, Ijuin M, Toita R, Jun B, Asai D, Mori T, Niidome T, Tanizawa K, Kuroda S, Katayama Y.
  • Journal Title: Nanomedicine Volume: 6 Pages: 583-589
  • Peer Reviewed
• [Journal Article] A gene delivery system specific for hepatoma cells and an intracellular kinase signal based on human liver-specific bionanocapsules and signal-responsive artificial polymer. (2010)
  • Author(s): Oishi J, Jung J, Tsuchiya A, Toita R, Kang JH, Mori T, Niidome T, Tanizawa K, Kuroda S, Katayama Y.
  • Journal Title: Int J Pharm. Volume: 396 Pages: 174-178
  • Peer Reviewed
• [Journal Article] Construction of protein-modified TiO2 nanoparticles for use with ultrasound irradiation in a novel cell-injuring method. (2010)
  • Author(s): Ogino C., Shibata N., Sasai R., Takaki K., Miyachi Y., Kuroda S., Ninomiya K., Shimizu N.
  • Journal Title: Bioorganic & Medicinal Chemistry Letters Volume: 20 Pages: 5320-5325
  • Peer Reviewed
• [Journal Article] Functions of fasciculation and elongation protein zeta-1 (FEZ1) in the brain. (2010)
  • Author(s): Maturana AD, Fujita T, Kuroda S.
  • Journal Title: Scientific World Journal. Volume: 10 Pages: 1646-1654
  • Peer Reviewed
• [Journal Article] 1分子1細飽アッセイ技術に基づく生物工学の最近の潮流 (2010)
  • Author(s): 黒田俊一、藤井郁夫、近藤昭彦
  • Journal Title: 生物工学会誌 Volume: 88 Pages: 671-671
• [Journal Article] Bio-nanocapsule-liposome conjugates for in vivo pinpoint drugs and gene delivery (2009)
  • Author(s): Kasuya, T., Jung, J., Kinoshita, R., Goh, Y., Matsuzaki, T., Iijima, M., Yoshimoto, N., Tanizawa, K., and Kuroda, S
  • Journal Title: Methods in Enzymology Volume: 464 Pages: 147-166
  • Peer Reviewed
• [Journal Article] Targeted nanoparticle drug delivery systems to the human liver: in vitro and in vivo advances (2009)
  • Author(s): Kasuya, T., Yamada, T., and Kuroda, S
  • Journal Title: Expert Opinion on Drug Delivery Volume: 6 Pages: 1-14
  • Peer Reviewed
• [Journal Article] ぶどう膜炎治療におけるドラッグデリバリーシステムの可能性 (2009)
  • Author(s): 真下 永、黒田俊一
  • Journal Title: 眼科 Volume: Vol. 51 Pages: 891-899
  • Peer Reviewed
• [Journal Article] Role of the T-type Calcium Channel Cav3.2 in the Chronotropic Action of Corticosteroids in Isolated Rat Ventricular Myocytes (2009)
  • Author(s): Maturana A, Lenglet S, Python M, 黒田俊一, Rossier M F
  • Journal Title: Endocrinology 150 Pages: 3726-3734
  • Peer Reviewed
• [Journal Article] Human eosinophil cationic protein enhances stress fiber formation in Balb/c 3T3 fibroblasts and differentiation of rat neonatal cardiomyocytes (2009)
  • Author(s): Fukuda T, Iwata M, Kitazoe M, Maeda T, Salomon D, Hirohata S, Tanizawa K, 黒田俊一, Seno M
  • Journal Title: Growth Factors 27 Pages: 228-236
  • Peer Reviewed
• [Journal Article] Accumulation of polyubiquitinated proteins by overexpression of RBCC protein interacting with protein kinase C2, a splice variant of ubiquitin ligase RBCC protein interacting with protein kinase C1 (2009)
  • Author(s): Yoshimoto N, Tatematsu K, Okajima T, Tanizawa K, 黒田俊一
  • Journal Title: FEBSJ 276 Pages: 6375-6385
  • Peer Reviewed
• [Journal Article] ぶどう膜炎治療におけるドラッグデリバリーシステムの可能性 (2009)
  • Author(s): 真下永, 黒田俊一
  • Journal Title: 眼科 51 Pages: 891-899
  • Peer Reviewed
• [Journal Article] Targeted nanoparticle drug delivery systems to the human liver : in vitro and in vivo advances (2009)
  • Author(s): Kasuya, T., Yamada, T., 黒田俊一
  • Journal Title: Expert Opinion on Drug Delivery 6 Pages: 1-14
  • Peer Reviewed
• [Journal Article] Bio-nanocapsule-liposome conjugates for in vivo pinpoint drugs and gene delivery (2009)
  • Author(s): Kasuya, T., Jung, J., Kinoshita, R., Goh, Y., Matsuzaki, T., Iijima, M., Yoshimoto, N., Tanizawa, K., 黒田俊一
  • Journal Title: Methods in Enzymology 464 Pages: 147-166
  • Peer Reviewed
• [Presentation] In vivo pinpoint gene and drug deliverly system using a hybrid of liposomes and HBV envelope proteins, bio-nanocapsules (2011)
  • Author(s): 黒田俊一
  • Organizer: 2011 International Symposium on Nanotechnology, From Bench To Clinic
  • Place of Presentation: ASAN Medical Center, ソウル、韓国
  • Year and Date: 2011-02-25
• [Presentation] In vivo pinpoint gene and drug deliverly system using a hybrid of liposomes and HBV envelope proteins, bio-nanocapsules (2011)
  • Author(s): 黒田俊一
  • Organizer: 2011 International Symposium on Nanotechnology, From Bench To Clinic
  • Place of Presentation: ASAN Medical Center,ソウル、韓国
  • Year and Date: 2011-02-25
• [Presentation] 能動的標的化DDSキャリア「バイオナノカプセル」とワクチン領域での応用について (2011)
  • Author(s): 黒田俊一
  • Organizer: 第32回獣医学・応用動物科学セミナー「ワクチンの科学:課題と試み」
  • Place of Presentation: 東京大学弥生講堂(東京都)
  • Year and Date: 2011-01-21
• [Presentation] バイオナノカプセル複合体化による遺伝子キャリアの高機能化 (2010)
  • Author(s): 黒田俊一
  • Organizer: 第33回日本分子生物学年会・第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-09
• [Presentation] バイオナノカプセル複合体化による遺伝子キャリアの高機能化 (2010)
  • Author(s): 黒田俊一
  • Organizer: 第33回日本分子生物学年会・第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] 骨分化誘導タンパク質NELL1の細胞接着活性とその下流シグナルの解析 (2010)
  • Author(s): 田島寛己, 新美友章, 井出照彦, 長谷部愛, 良元伸男, 黒田俊一
  • Organizer: 第33回日本分子生物学年会・第83回日本生化学会大会合同大会(BMB2010)
  • Place of Presentation: 神戸国際会議場(兵庫県)
  • Year and Date: 2010-12-09
• [Presentation] Development of DDS carrier utilizing BNC (bio-nanocapsule, a liposome possessing viral infection machinery) (2010)
  • Author(s): 黒田俊一
  • Organizer: The 65th West Lake International Symposium of Zhejiang University
  • Place of Presentation: 浙江大学、杭州、中国
  • Year and Date: 2010-11-20
• [Presentation] 全自動1細胞単離装置のバイオメディカル領域への応用 (2010)
  • Author(s): 黒田俊一, 良元伸男
  • Organizer: 第62回日本生物工学会
  • Place of Presentation: 宮崎シーガイア(宮崎県)
  • Year and Date: 2010-10-27
• [Presentation] 全自動1細胞単離装置の開発 (2010)
  • Author(s): 良元伸男, 藤井郁雄, 近藤昭彦, 黒田俊一
  • Organizer: 第62回日本生物工学会
  • Place of Presentation: 宮崎シーガイア(宮崎県)
  • Year and Date: 2010-10-27
• [Presentation] モノクローナル抗体を高分泌するハイブリドーマの全自動1細胞単離 (2010)
  • Author(s): 木田晶子, 良元伸男, 黒田俊一
  • Organizer: 第62回日本生物工学会
  • Place of Presentation: 宮崎シーガイア(宮崎県)
  • Year and Date: 2010-10-27
• [Presentation] バイオナノカプセルを用いた抗体の効率的整列化 (2010)
  • Author(s): 飯嶋益巳, 黒田俊一
  • Organizer: 第62回日本生物工学会
  • Place of Presentation: 宮崎シーガイア(宮崎県)
  • Year and Date: 2010-10-27
• [Presentation] 生体内ピンポイント遺伝子送達に適したバイオナノカプセル-リポプレックス複合体の高効率化 (2010)
  • Author(s): 太江田綾子, 山田光男, 良元伸男, 飯嶋益巳, 黒田俊一
  • Organizer: 第62回日本生物工学会
  • Place of Presentation: 宮崎シーガイア(宮崎県)
  • Year and Date: 2010-10-27
• [Presentation] Development of DDS carrier utilizing BNC (bio-nanocapsule, a liposome possessing viral infection machinery) (2010)
  • Author(s): 黒田俊一
  • Organizer: The 19th DDS Conference
  • Place of Presentation: 静岡グランメッセ
  • Year and Date: 2010-09-04
• [Presentation] Development of DDS carrier utilizing BNC (bio-nanocapsule, a liposome possessing viral infection machinery) (2010)
  • Author(s): 黒田俊一
  • Organizer: The 19th DDS Conference
  • Place of Presentation: 静岡グランメッセ(静岡県)
  • Year and Date: 2010-09-04
• [Presentation] 全自動1細胞単離装置による新しい細胞育種に関して (2010)
  • Author(s): 黒田俊一, 良元伸男
  • Organizer: 生物工学会中部支部例会
  • Place of Presentation: 名古屋大学(愛知県)
  • Year and Date: 2010-08-02
• [Presentation] Bio-nanocapsules for the in vivo pinpoint delivery of nucleic acids and drugs (2010)
  • Author(s): 黒田俊一
  • Organizer: ジュネーブ大学理学部主催 International Workshopon Nanomedicine
  • Place of Presentation: ジュネーブ大学理学部、スイス
  • Year and Date: 2010-06-01
• [Presentation] Bio-nanocapsules for the in vivo pinpoint delivery of nucleic acids and drugs (2010)
  • Author(s): 黒田俊一
  • Organizer: ジュネーブ大学理学部主催International Workshop on Nanomedicine
  • Place of Presentation: ジュネーブ大学理学部、スイス
  • Year and Date: 2010-06-01
• [Presentation] BIO-NANOCAPSULES FOR THE IN VIVO PINPOINT DELIVERY OF NUCLEIC ACIDS AND DRUGS (2010)
  • Author(s): 黒田俊一
  • Organizer: Particles2010
  • Place of Presentation: Orland, FA, USA
  • Year and Date: 2010-05-24
• [Presentation] Bio-nanocapsule-Lipoplex Complex for In Vivo Pinpoint Gene Delivery System (2010)
  • Author(s): 山田光男、太江田綾子、良元伸男、黒田俊一
  • Organizer: the LRD/LLMB 2010 conference
  • Place of Presentation: British Columbia Univ.Vancouver, Canada
• [Presentation] バイオナノカプセル・リポソーム複合体による DNA 及び siRNA デリバリー技術 (2009)
  • Author(s): 黒田俊一
  • Organizer: 日本 DDS 学会
  • Place of Presentation: 東京ドームホテル(東京)
  • Year and Date: 2009-07-03
• [Presentation] バイオナノカプセル・リポソーム複合体によるDNA及びsiRNAデリバリー技術 (2009)
  • Author(s): 黒田俊一
  • Organizer: 日本DDS学会
  • Place of Presentation: 東京ドームホテル (東京)
  • Year and Date: 2009-07-03
• [Book] 未来医療を支える先端バイオマテリアル～生体分子から有機・セラミック・金属まで～ (2012)
  • Author(s): 良元伸男、黒田俊一(監修 石原一彦、秋吉一成、山岡哲二)
  • Publisher: (株)NTS
• [Book] 生体内ピンポイント薬剤送達システム 「ウイルスとリポソームの長所を合わせ持つバイオナノカプセル」 (2011)
  • Author(s): 黒田俊一共創・協奏 産学連携成功のキーワード (独立行政法人科学技術振興機構JST イノベーションプラザ大阪 編)
• [Book] DDS 研究の進歩 XX 高度な肝臓細胞標的化能及び感染能を有するバイオナノカプセル-リポプレックス複合体の開発 (2011)
  • Author(s): 宮部康平,太江田綾子,山田光男,黒田俊一 (奥直人、山田静雄、賀川義之、板井茂、並木徳之 編集)
• [Book] 5th European Conference of the International Federation for Medical and Biological Engineering (Akos Jobbagy Ed.) IFMBE Proceedings Volume 37 Bio-nanocapsules for Oriented Immobilization of IgGs on Immunosensor Chips (2011)
  • Author(s): Masumi Iijima and Shun'ichi Kuroda(分担)
  • Publisher: Springer (Heidelberg, Germany)
• [Book] バイオナノカプセル(ウイルス由来感染機構を有するリポソーム)を用いる DDS キャリアの開発 (2010)
  • Author(s): 黒田俊一 DDS 研究の進歩 XIX (奥直人、山田静雄、賀川義之、板井茂、並木徳之 編集)
• [Book] 酵素活用ハンドブック(小宮山真監修、秋吉一成、中村聡、中村亨先生、津本浩平編集) (2010)
  • Author(s): 黒田俊一
  • Total Pages: 10
  • Publisher: NTS(東京)
• [Book] DDS研究の進歩XIX(奥直人、山田静雄、賀川義之、板井茂、並木徳之編集) (2010)
  • Author(s): 黒田俊一
  • Total Pages: 6
  • Publisher: 静岡DDS研究会
• [Book] (東京)中空バイオナノ粒子 (2009)
  • Author(s): 黒田俊一(分担) 超分子サイエンス(国武豊喜監修)
  • Publisher: (株)NTS
• [Book] 超分子サイエンス (国武豊喜監修) (2009)
  • Author(s): 黒田俊一
  • Total Pages: 15
  • Publisher: NTS (東京)
• [Remarks]
  • URL: http://kenpro.mynu.jp:8001/Profiles/0063/0006301/profile.html
• [Patent(Industrial Property Rights)] バイオナノカプセル及びリポソームを含む複合体 (2013)
  • Inventor(s): 黒田俊一、松尾英典、良元伸男、飯嶋益巳、新川武
  • Industrial Property Rights Holder: 名古屋大学、琉球大学
  • Filing Date: 2013-03-29
  • Overseas
• [Patent(Industrial Property Rights)] バイオナノカプセル及びリポソームを含む複合体 (2013)
  • Inventor(s): 黒田俊一、松尾英典、良元伸男、飯嶋益巳、新川武
  • Industrial Property Rights Holder: 名古屋大学、琉球大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2013-03-29
  • Overseas
• [Patent(Industrial Property Rights)] バイオナノカプセル及びリポソームを含む複合体 (2012)
  • Inventor(s): 黒田俊一、松尾英典、良元伸男、飯嶋益巳、新川武
  • Industrial Property Rights Holder: 名古屋大学、琉球大学
  • Industrial Property Number: 2012-080791
  • Filing Date: 2012-03-30
• [Patent(Industrial Property Rights)] バイオナノカプセルの効率的な精製方法 (2012)
  • Inventor(s): 黒田俊一、前川圭美、名木田真奈
  • Industrial Property Rights Holder: JST、大阪大学、ビークル
  • Acquisition Date: 2012-03-02
• [Patent(Industrial Property Rights)] バイオナノカプセルの効率的な精製方法 (2012)
  • Inventor(s): 黒田俊一、前川圭美、名木田真奈
  • Industrial Property Rights Holder: JST、大阪大学、ビークル
  • Acquisition Date: 2012-01-03
  • Overseas
• [Patent(Industrial Property Rights)] バイオナノカプセル及びリポソームを含む複合体 (2012)
  • Inventor(s): 黒田俊一、松尾英典、良元伸男、飯嶋益巳、新川武
  • Industrial Property Rights Holder: 名古屋大学、琉球大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-03-30
• [Patent(Industrial Property Rights)] ウイルス粒子様ナノカプセル (2011)
  • Inventor(s): 多田宏子、妹尾昌治、黒田俊一
  • Industrial Property Rights Holder: 岡山大学、名古屋大学
  • Industrial Property Number: 2011-162596
  • Filing Date: 2011-07-25
• [Patent(Industrial Property Rights)] 抗体を提示するタンパク質中空ナノ粒子を用いる治療薬剤およびタンパク質中空ナノ粒子 (2011)
  • Inventor(s): 黒田俊一、谷澤克行、近藤昭彦、上田政和、妹尾昌治、岡島俊英
  • Industrial Property Rights Holder: ビークル
  • Acquisition Date: 2011-05-31
  • Overseas
• [Patent(Industrial Property Rights)] ウイルス粒子様ナノカプセル (2011)
  • Inventor(s): 多田宏子、妹尾昌治、黒田俊一
  • Industrial Property Rights Holder: 岡山大学、名古屋大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2011-07-25
• [Patent(Industrial Property Rights)] リポソーム複合体、その製造方法、及びその使用 (2010)
  • Inventor(s): 黒田俊一,太江田綾子
  • Industrial Property Rights Holder: 黒田俊一,太江田綾子
  • Industrial Property Number: 2010-214303
  • Filing Date: 2010-09-24
• [Patent(Industrial Property Rights)] がん幹細胞の単離方法 (2010)
  • Inventor(s): 三善英知, 森脇健太, 奥戸久美子, 黒田俊一, 武石俊作
  • Industrial Property Rights Holder: 大阪大学、名古屋大学、(株)GPバイオサイエンス
  • Industrial Property Number: 2010-211216
  • Filing Date: 2010-09-21
• [Patent(Industrial Property Rights)] リポソーム複合体、その製造方法、及びその使用 (2010)
  • Inventor(s): 黒田俊一, 太江田綾子
  • Industrial Property Rights Holder: 名古屋大学
  • Industrial Property Number: 2010-214303
  • Filing Date: 2010-09-24
• [Patent(Industrial Property Rights)] タンパク質中空ナノ粒子とそれを用いた物質運搬体、ならびに細胞への物質導入方法 (2009)
  • Inventor(s): 黒田俊一、谷澤克行、妹尾昌治、近藤昭彦、上田政和
  • Industrial Property Rights Holder: ビークル
  • Acquisition Date: 2009-10-06
  • Overseas
• [Patent(Industrial Property Rights)] 細胞ピッキングシステム、スクリーニング方法および哺乳類細胞を取得する方法 (2009)
  • Inventor(s): 立松健司, 黒田俊一, 良元伸勇, 上向健司, 藤井郁夫
  • Industrial Property Rights Holder: アズワン株式会社、大阪大学、大阪府立大学
  • Industrial Property Number: 2009-133412
  • Filing Date: 2009-06-02
• [Patent(Industrial Property Rights)] タンパク質中空ナノ粒子とそれを用いた物質運搬体、ならびに細胞への物質導入方法 (2009)
  • Inventor(s): 黒田俊一, 谷澤克行, 妹尾昌治, 近藤昭彦, 上田政和
  • Industrial Property Rights Holder: (株) ビークル
  • Acquisition Date: 2009-10-06
  • Overseas
• [Patent(Industrial Property Rights)] 好酸球カチオン性タンパク質を含有する組成物 (2009)
  • Inventor(s): 妹尾昌治, 黒田俊一, 谷澤克行, 北添翠, 岩田美紀, 藤田敏次, 朴井伸行
  • Industrial Property Rights Holder: JST
  • Acquisition Date: 2009-11-20
• [Patent(Industrial Property Rights)] NELL peptide expression systems and bone formation activity of NELL peptide (2009)
  • Inventor(s): Kang Ting, 黒田俊一, Ben Wu
  • Industrial Property Rights Holder: 発明者と同一 (管理はUCLA)
  • Acquisition Date: 2009-06-09
  • Overseas