Development of high-performance nano-carrier for next generation medicine

• Project/Area Number: 21241029
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Nanomaterials/Nanobioscience
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: HANDA Hiroshi 東京工業大学, ソリューション研究機構, 教授 (80107432)
• Co-Investigator(Kenkyū-buntansha): OCHIAI Takahiro 国立がんセンター(研究所及び東病院臨床開発センター), がん転移研究室, 独立室長 (60192530) ; AOKI Ichio 独立行政法人放射線医学総合研究所, 分子イメージング研究センター, チームリーダー (10319519)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥46,800,000 (Direct Cost: ¥36,000,000、Indirect Cost: ¥10,800,000); Fiscal Year 2011: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000); Fiscal Year 2010: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000); Fiscal Year 2009: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
• Keywords: 高機能性ナノカプセル / ウイルス外殻タンパク質 / 自己集合化 / 新規DDS用キャリア / ウイルス外郭タンパク質 / ウイルス様粒子 / ナノカプセル / 高機能化 / MRI造影剤 / アジュバント効果 / ワクチン製剤 / バイオテクノロジー / ウイルス / ナノ材料 / ナノバイオ / 癌 / DDS / 外殻タンパク質 / 自己組織化

Research Abstract

We havedevelopednovel technologiesonformationofnanocapsules throughself-assembly activity of SV40major capsid protein, introduction of biologically active agents into nanocapsules and modification or alteration of nanocapsule surface, and constructed high-perfor mance nanocapsules used as an effective DDS carrier for medical application.

Research Products

• [Journal Article] Identification of NIPSNAP1 anocistatin-interacting prot(as) ein involving pain transmission (2012)
  • Author(s): Okuda-Ashitaka, E., Minami, T., Tsubouchi, S., Kiyonari, H., Iwamatsu, A., Noda, T., Handa, H. and Ito, S.
  • Journal Title: J. Biol. Chem Volume: 287 Pages: 10403-10413
• [Journal Article] Tools and methodologies capable of isolating and identifying a target molecule for bioactive compound (2012)
  • Author(s): akamoto, S., Hatakeyama, M., Ito, T. andHanda, H.
  • Journal Title: (review) Bioorg.(a) Med. Chem Volume: 20 Pages: 1990-2001
• [Journal Article] Identification of NIPSNAP1 as a nocistatin-interacting protein involving pain transmission (2012)
  • Author(s): Okuda-Ashitaka
  • Journal Title: J.Biol.Chem. Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] In vitro reconstitution of SV40 particles that are composed of VP1/2/3 capsid proteins and nucleosomal DNA and direct efficient gene transfer (2011)
  • Author(s): Enomoto, T., Kukimoto I., Kawano, M., Yamaguchi, Y., Berk, A. J. and Handa, H.
  • Journal Title: Virology Volume: 420 Pages: 1-9
• [Journal Article] Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor (2011)
  • Author(s): Kitai, Y., Fukuda, H., Enomoto, T., Asakawa, Y., Suzuki, T., Inouye, S. and Handa, H.
  • Journal Title: J. Biotechnol Volume: 155 Pages: 251-256
• [Journal Article] A two-step ligand exchange reaction generates highly water-dispersed magnetic nanoparticles for biomedical applications (2011)
  • Author(s): Hatakeyama, M., Kishi, H., Kita, Y., Imai, K., Nishio, K., Karasawa, S., Masaike, Y., Akamoto, S., Sandhu, A., Tanimoto, A., Gomi, T., Kohda, E., Abe, M. and Handa, H.
  • Journal Title: J. Mater. Chem Volume: 21 Pages: 5959-5966
• [Journal Article] Cell selective targeting of a simian virus 40 virus-like particle conjugated to epidermal growth factor (2011)
  • Author(s): Kitai-Y
  • Journal Title: J.Biotechnol. Volume: 155 Pages: 251-256
  • Peer Reviewed
• [Journal Article] Synthesis and applications of magnetic nanoparticles for biorecognition and point of care medical diagnostics (2010)
  • Author(s): Sandhu, A., Handa, H. and Abe, M.
  • Journal Title: (review) Nanotechnology Volume: 21 Pages: 442001-442022
• [Journal Article] Identification of a primary target of thalidomide teratogenicity (2010)
  • Author(s): Ito, T., Ando, H., Suzuki, T., Ogura, T., Hotta, K., Imamura, Y., Yamaguchi, Y. and Handa, H.
  • Journal Title: Science Volume: 327 Pages: 1345-1350
• [Journal Article] Magneto-optical biosensing platform based on light scattering from self-assembled chains of functionalized rotating magnetic beads (2010)
  • Author(s): Park, S. Y., Handa, H. and Sandhu, A.
  • Journal Title: Nano Lett Volume: 10 Pages: 446-451
• [Journal Article] Synthesis and applications of magnetic nanoparticles for biorecognition and point of care medical diagnostics. (review) (2010)
  • Author(s): Sandhu, A., Handa, H., Abe, M.
  • Journal Title: Nanotechnology Volume: 21
  • Peer Reviewed
• [Journal Article] Identification of a primary target of thalidomide teratogenicity. (2010)
  • Author(s): Ito, T., Ando, H., Suzuki, T., Ogura, T., Hotta, K., Imamura, Y., Yamaguchi, Y., Handa, H.
  • Journal Title: Science Volume: 327 Pages: 1345-1350
  • Peer Reviewed
• [Journal Article] Magneto-optical biosensing platform based on light scattering from selfassembled chains of functionalized rotating magnetic beads. (2010)
  • Author(s): Park, S.Y., Handa, H., Sandhu, A.
  • Journal Title: Nano Lett Volume: 10 Pages: 446-451
  • Peer Reviewed
• [Journal Article] Calcium-bridge triggers capsid disassembly in the cell entry process of simian virus 40 (2009)
  • Author(s): Kawano, M-K., Xing, L., Tsukamoto, H., Inoue, T., Handa, H. and Cheng, R. H.
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 34703-34712
• [Journal Article] DSIF, the Paf1 complex, and Tat-SF1 have non-redundant cooperative roles in RNA polymerase II elongation (2009)
  • Author(s): Chen, Y., Yamaguichi, Y., Tsugeno, Y., Yamamoto, J., Yamada, t., Nakamura, M., Hisatake, K. and Handa, H.
  • Journal Title: Genes Dev Volume: 23 Pages: 2765-2777
• [Journal Article] Cellular dynamics of the negative transcription elongation factor NELF (2009)
  • Author(s): Yung, T. M. C., Narita, T., Komori, T., Yamaguchi, Y. and Handa, H.
  • Journal Title: Exp. Cell Res Volume: 315 Pages: 1693-1705
• [Journal Article] Development and application of high-performance affinity beads : toward chemical biology and drug discovery(review) (2009)
  • Author(s): Sakamoto, S., Kabe, Y., Hatakeyama, M., Yamaguchi, Y. and Handa, H.
  • Journal Title: Chem. Rec Volume: 9 Pages: 66-85
• [Journal Article] Preparation and medical application of magnetic beads conjugated with bioactive molecules. (2009)
  • Author(s): Abe, M., Nishio, K., Hatakeyama.M., Hanyu, N., Tanaka, T., Nakagawa, T., Sandhu, A., Handa, H.
  • Journal Title: J.Magn.Magn.Mater. 321 Pages: 645-649
  • Peer Reviewed
• [Journal Article] Calcium-bridge triggers capsid disassembly in the cell entry process of simian virus 40. (2009)
  • Author(s): Kawano, M-K., Xing, L., Tsukamoto, H., Inoue, T., Hand, H., Cheng, R.H.
  • Journal Title: J.Biol.Chem. 284 Pages: 34703-34712
  • Peer Reviewed
• [Journal Article] Magneto-optical biosensing platform based on light scattering from self-assembled chains of functionalized rotating magnetic beads. (2009)
  • Author(s): Park, S.Y., Handa, H., Sandhu, A.
  • Journal Title: Nano Lett. 10 Pages: 446-451
  • Peer Reviewed
• [Presentation] Development of a novel platfom for CTL-based influenza vaccine using virus like particles of simian virus 40 (2011)
  • Author(s): Masaaki Kawano, Tatsuya Suda, Toshitaka Akatsuka, Hiroahi Handa and Masanori Matsui
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2011-09-13
• [Presentation] ウィルスカプシドタンパク質を利用したDDS用医療材料の創製 (2011)
  • Author(s): 半田宏
  • Organizer: DDS学会
  • Place of Presentation: 東京大学本郷キャンパス
  • Year and Date: 2011-06-09
• [Presentation] ウィルスカプシドタンパク質を利用したDDS用医療材料の創製 (2011)
  • Author(s): 半田宏
  • Organizer: DDS学会
  • Place of Presentation: 東京大学本郷キャンパス(招待講演)
  • Year and Date: 2011-06-09
• [Presentation] In vitro reconstitution of SV40 particles that are composed of VP1/2/3 capsid proteins and nucleosomal DNA and direct efficient gene transfer (2011)
  • Author(s): Teruya Enomoto, Yuki Yamagchi, Iwao Kukimoto, Arnold J. Berk, Hiroshi Handa
  • Organizer: International Centre for Genetic Engineering and Biotechology
  • Place of Presentation: Stazione Marittima Congress Centre, Trieste, Italy
• [Presentation] In vitro reconstitution of SV40 particles that are composed of VP1/2/3 capsid proteins and nucleosomal DNA and direct efficient gene transfer (2011)
  • Author(s): 榎本輝也
  • Organizer: ICGEB DNA Tumour Virus Meeting
  • Place of Presentation: International Centre for Genetic Engineering and Biotechology
• [Presentation] 新規高機能性蛍光・磁性ビーズの作製と応用 (2010)
  • Author(s): 坂本聡, 望月勇輔, 河田慎太郎, 内藤靖之, 畠山士, 半田宏
  • Organizer: 高分子ミクロスフェア討論会運営委員会
  • Place of Presentation: 福井大学総合研究棟13階会議室
  • Year and Date: 2010-11-08
• [Presentation] ウイルスカプシドタンパク質の自己集合化によるナノカプセル形成技術の開発と応用 (2010)
  • Author(s): 半田宏
  • Organizer: 分子ロボティクス研究会
  • Place of Presentation: 東工大田町キャンパス
  • Year and Date: 2010-04-16
• [Presentation] ウイルス外殻タンパク質を利用したDDSキャリアーの開発 (2009)
  • Author(s): 土井浩司、榎本輝也、半田宏
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] MRI造影剤としての腫瘍標的化フェライト封入ウイルス様粒子の加工 (2009)
  • Author(s): 福田元、榎本輝也、土井浩二、青木伊知男、半田宏
  • Organizer: 日本分子生物学会
  • Place of Presentation: パシフィコ横浜
• [Presentation] SV40キャプシドタンパク質の自己集合化による遺伝子導入用ナノカプセルの構築 (2009)
  • Author(s): 榎本輝也、川野雅章、井上隆昌、高橋陵宇、半田宏
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 都市センターホテル
• [Presentation] 遺伝子治療を目指した表面改変型SV40様粒子の開発 (2009)
  • Author(s): 土井浩司、高橋陵宇、榎本輝也、半田宏
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 都市センターホテル
• [Presentation] フェライトを包含したSV40ウイルス様粒子による癌診断プローブ開発 (2009)
  • Author(s): 福田元、榎本輝也、高橋陵宇、土井浩二、澤田航、半田宏
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 都市センターホテル
• [Presentation] SV40キャプシドタンパク質の自己集合化による遺伝子導入用ナノカプセルの構築 (2009)
  • Author(s): 榎本輝也、川野雅章、井上隆昌、高橋陵宇、半田宏
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 都市センターホテル
• [Book] ナノ融合による先進バイオデバイス(高機能性ナノ磁性粒子と医療・バイオへの応用) (2011)
  • Author(s): 畠山士、半田宏
  • Publisher: シーエムシー出版
• [Book] From Discovery to Clinical Testing(Design Platforms of Nanocapsules for Human Therapeutics or Vaccines) (2011)
  • Author(s): Masaaki Kawano, Li Xing, Kit S. Lam, Hiroshi Handa, Tatsuo Miyamura, Susan Barnett, Indresh K. Srivastava, and R. Holland Cheng Jhon Wiley & Sons.
  • Publisher: Development of Vaccines
• [Book] Jhon Wiley & Sons.Inc. (2011)
  • Author(s): Masaaki Kawano
  • Publisher: Design Platforms of Nanocapsules for Human Therapeutics or Vaccines, 125
• [Book] ナノ粒子・マイクロ粒子の調製と応用技術(創薬に向けた磁気アフェニティビーズの創製) (2010)
  • Author(s): 壺内信吾,西尾広介,池田森人,成松宏樹,郷右近展之,半田宏
  • Publisher: シーエムシー出版
• [Book] コアシェル微粒子の設計・合成技術・応用の展開 (2010)
  • Author(s): 畠山士、半田宏(監修:川口春馬)
  • Total Pages: 11
  • Publisher: シーエムシー出版
• [Book] MEMS/NEMS工学全集(診断学, 1. MEMSと診断他) (2009)
  • Author(s): 半田宏、和田忠士、坂本聡
  • Publisher: 株式会社テクノシステム
• [Book] バイオ研究のフロンティア医療・診断をめざす先端バイオテクノロジー(1.機能性ナノ磁性ビーズ) (2009)
  • Author(s): 半田宏
  • Publisher: 工学図書株式会社
• [Book] PROTEIN TARGETING WITH SMALL MOLECULES, Chemical Biology Techniques and Applications (2009)
  • Author(s): Yasuaki Kabe, Mamoru Hatakeyama, Satoshi Sakamoto, Kousuke Nishio, Hiroshi Handa
  • Total Pages: 289
  • Publisher: Jhon Wiley & Sons.Inc.
• [Remarks]
  • URL: http://www.hands.bio.titech.ac.jp/j/j-home.html
• [Remarks]
  • URL: http://www.handa.bio.titech.ac.jp/j/j-home.html
• [Patent(Industrial Property Rights)] 細胞傷害性T細胞誘導剤 (2011)
  • Inventor(s): 半田宏、川野雅章、松井政則
  • Industrial Property Rights Holder: 東京工業大学・埼玉医科大学
  • Industrial Property Number: 2011-191208
  • Filing Date: 2011-09-02
• [Patent(Industrial Property Rights)] 標識磁性粒子を用いた被検物質の検出方法、及び被検物質の検出システム (2011)
  • Inventor(s): 半田宏、坂本聡、河田慎太郎、内藤靖之、凸版{平瀬匠、勝又優、土屋真紀子、星野昭裕、入江新司、中山雅人}
  • Industrial Property Rights Holder: 東京工業大学・凸版印刷株式会社
  • Industrial Property Number: 2011-053415
  • Filing Date: 2011-03-10
• [Patent(Industrial Property Rights)] ポリマー被覆強磁性粒子の製造方法及びポリマー被覆強磁性粒子(Process for producing polymer-coated ferromagnetic particle, and polymer-coated ferromagnetic particle) (2011)
  • Inventor(s): 半田宏、西尾広介、郷右近展之、阿部正紀
  • Industrial Property Rights Holder: 東京工業大学
  • Acquisition Date: 2011-11-30
  • Overseas
• [Patent(Industrial Property Rights)] 改変されたウイルスカプシドタンパク質及びその使用 (2011)
  • Inventor(s): 半田宏、中西章、金刺進之介、高橋陵宇
  • Industrial Property Rights Holder: 東京工業大学
  • Acquisition Date: 2011-09-09
• [Patent(Industrial Property Rights)] 生理的条件化でのウイルス粒子様構造体及びその形成方法 (2011)
  • Inventor(s): 半田宏、中西章、川野雅章
  • Industrial Property Rights Holder: 東京工業大学
  • Acquisition Date: 2011-05-13
• [Patent(Industrial Property Rights)] 細胞障害性T細胞誘導剤 (2011)
  • Inventor(s): 半田宏
  • Industrial Property Rights Holder: 東京工業大学大学, 埼玉医科大学
  • Industrial Property Number: 2011-191208
  • Filing Date: 2011-09-02
• [Patent(Industrial Property Rights)] ポリマー被覆フェライト微粒子および製造方法 (2010)
  • Inventor(s): 半田宏、畠山士、坂本聡、岸寛
  • Industrial Property Rights Holder: 東京工業大学
  • Filing Date: 2010-10-28
  • Overseas
• [Patent(Industrial Property Rights)] ウイルス外殻タンパク質被覆構造体及びその製造方法 (2010)
  • Inventor(s): 半田宏、土井浩司、榎本輝也、川野雅章
  • Industrial Property Rights Holder: 東京工業大学
  • Filing Date: 2010-08-30
  • Overseas
• [Patent(Industrial Property Rights)] ウイルス外殻構成タンパク質被膜構造体及びその製造方法 (2010)
  • Inventor(s): 半田宏、土井浩司、榎本輝也、川野雅章
  • Industrial Property Rights Holder: 東京工業大学
  • Filing Date: 2010-08-30
  • Overseas
• [Patent(Industrial Property Rights)] 磁気温熱療法用発熱体および製造方法 (2009)
  • Inventor(s): 阿部正紀、中川貴、多田大、山本修平、半田宏
  • Industrial Property Rights Holder: 東京工業大学
  • Industrial Property Number: 2009-181597
  • Filing Date: 2009-08-04
• [Patent(Industrial Property Rights)] ウィルス外殻タンパク質被覆構造体及びその製造方法 (2009)
  • Inventor(s): 半田宏、土井浩司、榎本輝也
  • Industrial Property Rights Holder: 東京工業大学
  • Industrial Property Number: 2009-207632
  • Filing Date: 2009-09-09