Development of novel nucleic acid materials that efficiently work in the intracellular molecular environment
| Project/Area Number |
21245040
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Konan University |
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Principal Investigator |
SUGIMOTO Naoki 甲南大学, フロンティアサイエンス学部, 教授 (60206430)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI Junji 甲南大学, フロンティアサイエンス学部, 教授 (40289012)
NAKANO Shu-ichi 甲南大学, フロンティアサイエンス学部, 准教授 (70340908)
MIYOSHI Daisuke 甲南大学, フロンティアサイエンス学部, 准教授 (50388758)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥23,530,000 (Direct Cost: ¥18,100,000、Indirect Cost: ¥5,430,000)
Fiscal Year 2011: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2010: ¥12,870,000 (Direct Cost: ¥9,900,000、Indirect Cost: ¥2,970,000)
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| Keywords | 核酸 / 四重鎖構造 / 相互作用エネルギー / リボザイム / 分子クラウディング / ポリエチレングリコール / 四重らせん構造 / 熱力学的安定性 / DNAチップ / ヒストンペプチド / アプタマー / PCR / バイオセンサー / DNA / ラロメア配列 / 枝分かれ構造 / 金属イオン |
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| Research Abstract |
We studied nucleic acid interactions and reactions by using a model experimental system that mimics the intracellular molecular environment. This research provided quantitative insights into influences of the molecular crowding medium on nucleic acid folding, a ribozyme activity, and interactions with proteins and peptides. It is supposed that these data can bridge the gap between the results obtained in vitro and in cells. Based on results regarding the effects of molecular environment, we developed novel nucleic acid materials and sensing methods that efficiently work under unusual conditions such as in living cells.
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Report
(4 results)
Research Products
(118 results)
