Induction mechanism of imposex caused by organotin compounds in gastropods : elucidation of involvement of the retinoid X receptor

• Project/Area Number: 21248026
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: HORIGUCHI Toshihiro 独立行政法人国立環境研究所, 環境リスク研究センター, 室長 (30260186)
• Co-Investigator(Kenkyū-buntansha): OTA Yasuhiko 鳥取大学, 農学部, 教授 (60069078) ; IGUCHI Taisen 大学共同利用機関法人自然科学研究機構, 岡崎統合バイオサイエンスセンター, 教授 (90128588) ; MORISHITA Fumihiro 広島大学, 理学研究科, 助教 (20210164)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥36,660,000 (Direct Cost: ¥28,200,000、Indirect Cost: ¥8,460,000); Fiscal Year 2011: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000); Fiscal Year 2010: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000); Fiscal Year 2009: ¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
• Keywords: インポセックス / 有機スズ / レチノイドX受容体 / 神経ペプチド / ステロイド

Research Abstract

We isolated two isoforms of RXR cDNAs, RXR isoform 1(TcRXR-1) and RXR isoform 2(TcRXR-2), in the rock shell Thais clavigera. The deduced amino acid sequences of TcRXR-1 and TcRXR-2 are highly homologous with those of other gastropods. These TcRXR isoforms displayed 9cRA-dependent activation of transcription in a reporter gene assay using COS-1 cells. The transcriptional activity of TcRXR-2, the product of which has five additional amino acids in the T-box of the C domain, was significantly lower than that of TcRXR-1.Decreases of the transcriptional activity by TcRXR-1 were observed when more than equal amount of TcRXR-2 was existed in a co-transfection assay. Meanwhile, the characteristics of the development of male genitalia(penis and vas deferens) in imposex-exhibiting female T. clavigera, were histologically examined using specimens from a wild population and tributyltin(TBT)-exposed females in the laboratory. A variety of vas deferens morphogenesis patterns were observed in wild female T. clavigera, and the characteristics were summarised. The immature vas deferens at an initial stage, however, was only observed beneath or behind the penis, and no vas deferens was observed close to the vaginal opening(i. e., vulva) of the capsule gland in TBT-exposed females, which was different from the characteristics of vas deferens formation observed in wild females. Taking into consideration both the observed results from wild female specimens and from TBT-exposed females in the laboratory, the vas deferens sequence(VDS) index for T. clavigera was proposed as VDS 1-6.

Research Products

• [Journal Article] Vas deferens and penis development in the imposex-exhibiting female rock shell (2012)
  • Author(s): Horiguchi, T., Ohta, Y., Urushitani, H., Lee, J. H., Park, J. C., Cho, H. S., Shiraishi, H.
  • Journal Title: Thais clavigera, Marine Environmental Research Volume: VOL. 76 Pages: 71-79
  • Peer Reviewed
• [Journal Article] Specific accumulation of organotin compounds in tissues of the rock shell (2012)
  • Author(s): Horiguchi, T., Lee, J. H., Park, J. C., Cho, H. S., Shiraishi, H., Morita, M.
  • Journal Title: Thais clavigera, Marine Environmental Research Volume: VOL. 76 Pages: 56-62
  • Peer Reviewed
• [Journal Article] Vas deferens and penis development in the imposex-exhibiting female rock shell, Thais clavigera (2012)
  • Author(s): Horiguchi, T., Ohta, Y., Urushitani, H., Lee, J.H., Park, J.C., Cho, H.S., Shiraishi, H.
  • Journal Title: Marine Environmental Research Volume: VOL.76 Pages: 71-79
  • DOI: 10.1016/j.marenvres.2011.10.001
  • Peer Reviewed
• [Journal Article] Specific accumulation of organotin compounds in tissues of the rock shell, Thais clavigera (2012)
  • Author(s): Horiguchi, T., Lee, J.H., Park, J.C., Cho, H.S., Shiraishi, H., Morita, M.
  • Journal Title: Marine Environmental Research Volume: VOL.76 Pages: 56-62
  • DOI: 10.1016/j.marenvres.2011.09.002
  • Peer Reviewed
• [Journal Article] Immune gene mining by pyrosequencing the rockshell, Thais clavigera (2012)
  • Author(s): Rhee, J.S., Kim, B.M., Horiguchi, T., Lee, Y.M., Lee, J.S.
  • Journal Title: Fish & Shellfish Immunology Volume: VOL.32 Issue: 5 Pages: 700-710
  • DOI: 10.1016/j.fsi.2012.01.017
  • Peer Reviewed
• [Journal Article] Cloning and characterization of retinoid X receptor(RXR) isoforms in the rock shell (2011)
  • Author(s): Urushitani, H., Katsu, Y., Ohta, Y., Shiraishi, H., Iguchi, T., Horiguchi, T.
  • Journal Title: Thais clavigera, Aquatic Toxicology Volume: VOL. 103 Pages: 101-111
  • Peer Reviewed
• [Journal Article] Cloning and characterization of retinoid X receptor (RXR) isoforms in the rock shell, Thais clavigera. (2011)
  • Author(s): Urushitani, H., Katsu, Y.Ohta, Y., Shiraishi, H., Iguchi, T., Horiguchi.T
  • Journal Title: Aquatic Toxicology Volume: 103 Pages: 101-111
  • Peer Reviewed
• [Journal Article] Time course of expression of the retinoid X receptor gene and induction of imposex in the rock shell, Thais clavigera, exposed to triphenyltin chloride (2010)
  • Author(s): Horiguchi, T., Nishikawa, T., Ohta, Y., Shiraishi, H., Morita, M.
  • Journal Title: Analytical & Bioanalytical Chemistry Volume: VOL. 396 Pages: 597-607
  • Peer Reviewed
• [Journal Article] Establishment of a polyclonal antibody against the retinoid X receptor of the rock shell Thais clavigera and its application to rock shell tissues for imposex research (2010)
  • Author(s): Horiguchi, T., Urushitani, H., Ohta, Y., Iguchi, T., Shiraishi, H.
  • Journal Title: Ecotoxicology Volume: VOL. 19 Pages: 571-576
  • Peer Reviewed
• [Journal Article] Time course of expression of the retinoid X receptor gene and induction of imposex in the rock shell, Thais clavigera, exposed to triphenyltin chloride (2010)
  • Author(s): Horiguchi, T., Nishikawa, T., Ohta, Y., Shiraishi, H., Morita, M.
  • Journal Title: Analytical & Bioanalytical Chemistry 396 Pages: 597-607
  • Peer Reviewed
• [Journal Article] Establishment of a polyclonal antibody against the retinoid X receptor of the rock shell Thais clavigera and its application to rock shell tissues for imposex research (2010)
  • Author(s): Horiguchi, T., Urushitani, H., Ohta, Y., Iguchi, T., Shiraishi, H.
  • Journal Title: Ecotoxicology 19 Pages: 571-576
  • Peer Reviewed
• [Journal Article] Mitochondrial genome of Thais clavigera(Mollusca: Gastropoda): affirmation of the conserved, ancestral gene pattern within the mollusks (2010)
  • Author(s): Ki, J.S., Lee, Y.M., Jung, S.O., Horiguchi, T., Cho, H.S., Lee, J.S.
  • Journal Title: Molecular Phylogenetics and Evolution 54 Pages: 1016-1020
  • Peer Reviewed
• [Presentation] Vas deferens and penis development in the imposex-exhibiting female rock shell, Thais clavigera (2011)
  • Author(s): Horiguchi, T., Ohta, Y., Urushitani, H., Lee, J.H., Park, J.C., Cho, H.S., Shiraishi, H.
  • Organizer: 環境ホルモン学会第14回研究発表会
  • Place of Presentation: 東京大学山上会館(東京都)
  • Year and Date: 2011-12-01
• [Presentation] Development of genitalia in the ivory shell(Babylonia japonica): observation of the specimens from wild populations and laboratory-reared juveniles (2011)
  • Author(s): Horiguchi T., Ohta Y., Hamada F., Shiraishi H.
  • Organizer: SETAC(Society of Environmental Toxicology and Chemistry) Europe 21^<st> Annual Meeting
  • Place of Presentation: Milano Convention Centre Milano(Italy)
  • Year and Date: 2011-05-19
• [Presentation] Development of genitalia in the ivory shell (Babylonia japonica) : observation of the specimens from wild populations and laboratory-reared juveniles (2011)
  • Author(s): Horiguchi T., Ohta Y., Hamad F., Shiraishi H.
  • Organizer: SETAC (Society of Environmental Toxicology and Chemistry) Europe 21^<st> Annual Meeting
  • Place of Presentation: the Milano Convention Centre Milano (Italy)
  • Year and Date: 2011-05-19
• [Presentation] インポセックス研究の変遷とRXR仮説の展開 (2010)
  • Author(s): 堀口敏宏
  • Organizer: 環境ホルモン学会第13回研究発表会
  • Place of Presentation: 東京大学山上会館(東京都)
  • Year and Date: 2010-12-16
• [Presentation] イボニシ貝新規RXR遺伝子を用いたレポータージーンアッセイ法等による性状決定 (2010)
  • Author(s): 漆谷博志・勝義直・太田康彦・井口泰泉・白石寛明・堀口敏宏
  • Organizer: 環境ホルモン学会第13回研究発表会
  • Place of Presentation: 東京大学山上会館(東京都)
  • Year and Date: 2010-12-16
• [Presentation] Analysis of the retinoid X receptor(RXR)-mediated mechanism of imposex induced by organotins in gastropods (2010)
  • Author(s): Horiguchi, T., Ohta, Y., Urushitani, H., Iguchi, T., Nishikawa, J., Shiraishi, F., Shimizu, M., Morita, M., Shiraishi, H.
  • Organizer: Physical and Chemical Impacts on Marine Organisms-For Conservation of Biodiversity and Sustainability-
  • Place of Presentation: 愛知県立大学(愛知県)
  • Year and Date: 2010-10-25
• [Presentation] Analysis of the retinoid X receptor(RXR)-mediated mechanism of imposex induced by organotins in gastropods (2010)
  • Author(s): Horiguchi, T., Ohta, Y., Urushitani, H., Iguchi, T., Nishikawa, J., Shiraishi, F., Shimizu, M., Morita, M., Shiraishi, H.
  • Organizer: Physical and Chemical Impacts on Marine Organisms-For Conservation of Biodiversity and Sustainability
  • Place of Presentation: 愛知県立大学(愛知県)
  • Year and Date: 2010-10-25
• [Presentation] Developmental process of vas deferens and penis in the imposex-exhibiting female rock shell, Thais clavigera (2010)
  • Author(s): Horiguchi, T., Ohta, Y., Cho, H. S., Shiraishi, H.
  • Organizer: SETAC(Society of Environmental Toxicology and Chemistry) Europe 20^<th> Annual Meeting
  • Place of Presentation: Palacio de Congresosy Exposiciones-FIBES(Spain)
  • Year and Date: 2010-05-24
• [Presentation] Developmental process of vas deferens and penis in the imposex-exhibiting female rock shell, Thais clavigera (2010)
  • Author(s): Horiguchi, T., Ohta, Y., Cho, H.S., Shiraishi, H.
  • Organizer: SETAC (Society of Environmental Toxicology and Chemistry) Europe 20^<th> Annual Meeting
  • Place of Presentation: Palacio de Congresos y Exposiciones-FIBES (Spain)
  • Year and Date: 2010-05-24
• [Presentation] Recent trends of imposex and organotins content in the rock shell, Thais clavigera from Korean coast (2009)
  • Author(s): Cho, H. S., Park, J. C., Choi, M. K., Choi, H. G., Horiguchi, T.
  • Organizer: 第18回環境化学討論会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2009-06-10
• [Presentation] Imposex and organotin concentrations in Thais orbita and Morula marginalba around Sydney, NSW, Australia (2009)
  • Author(s): Horiguchi, T., Colville, A., Hamilton, L., Shiraishi, F., Nakajima, D., Kamata, R., Lim, R., Shiraishi, H.
  • Organizer: 第18回環境化学討論会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2009-06-09
• [Book] UAE, e-book Biochemical and Biological Effects of Organotins (2012)
  • Author(s): Horiguchi, T.(Pagliarani, A., Trombetti, F., Ventrella, V., eds.)
  • Total Pages: 204
  • Publisher: Bentham Science Publishers
• [Book] e-book Biochemical and Biological Effects of Organotins (eISBN : 978-1-60805-265-3 ; doi : 10.2174/97816080526531120101) (2012)
  • Author(s): Horiguchi, T.
  • Publisher: Bentham Science Publishers, UAE
• [Book] [新通史]日本の科学技術(全4巻+別巻1),第4巻第8部生命・環境・安全・防災 (2011)
  • Author(s): 堀口敏宏
  • Total Pages: 642
  • Publisher: 原書房,東京
• [Book] Interdisciplinary Studies on Environmental Chemistry-Biological Responses to Contaminants- (2010)
  • Author(s): Sousa, A., Barroso, C.M., Tanabe, S.Horiguchi, T.(Hamamura, N., Suzuki, S., Mendo, S., Barroso, C.M., Iwata, H., Tanabe, S.eds.)
  • Publisher: TERRAPUB, Tokyo, Japan
• [Book] Ecotoxicology of Antifouling Biocides (2009)
  • Author(s): Horiguchi, T.(Arai, T., Harino, H., Ohji, M., Langston, W. J., eds.)
  • Total Pages: 437
  • Publisher: Springer
• [Book] Ecotoxicology of Antifouling Biocides (2009)
  • Author(s): Horiguchi, et al.(Arai, T, Harino, H, Ohji, M, Langston, WJ eds.)
  • Total Pages: 437
  • Publisher: Springer, Tokyo, Japan
• [Remarks]
  • URL: http://db-in.nies.go.jp/research/rsdb_out/vdetail.php?id=100079