Analysis of non-olfactory sensory pathways that are necessary for the olfactory associative learning of the Drosophila mushroom body

• Project/Area Number: 21300117
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: ITO Kei 東京大学, 分子細胞生物学研究所, 准教授 (00311192)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2009: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
• Keywords: ショウジョウバエ / 嗅覚系 / 味覚系 / 投射神経 / 情報の統合 / 学習記憶 / GAL4エンハンサートラップ / LexAエンハンサートラップ

Research Abstract

The mushroom body is known to be an important center for associative olfactory memory in the insect brain. To understand the neural pathways of information other than the olfactory signals toward this center, we first analyzed the organization of the primary gustatory center, whose structure was hardly known despite the importance of gustatory information in associative olfactory learning. We also analyzed the neural connections of the brain regions that receive the arborizations of the input/output neurons of the mushroom body. By systematically labeling neurons according to cell lineage, we found that no neurons from the gustatory, auditory and visual centers directly project to these regions and that there are distinct regions that receive the projections from putative higher-associative centers.

Research Products

• [Journal Article] Chromatin modification of Notch targets in olfactory receptor neurondiversification (2012)
  • Author(s): Endo, K., Karim, M. R., Taniguchi, H., Krejci, A., Kinameri, E., Siebert, M., Ito, K., Bray, S. J., Moore, A. W.
  • Journal Title: Nat Neurosci(15) Pages: 224-233
• [Journal Article] Chromatin modification of Notch targets in olfactory receptor neuron diversification (2012)
  • Author(s): Endo, K., Karim, M.R., Taniguchi, H., Krejci, A., Kinameri, E., Siebert, M., Ito, K., Bray, S.J., Moore, A.W.
  • Journal Title: Nat Neurosci Volume: 15 Issue: 2 Pages: 224-233
  • DOI: 10.1038/nn.2998
  • Peer Reviewed
• [Journal Article] Flybrain neuron database : acomprehensive database system of theDrosophila brain neurons (2011)
  • Author(s): Shinomiya, K., Matsuda, K., Oishi, T., Otsuna, H., and Ito, K.
  • Journal Title: J Comp Neurol(519) Pages: 807-833
• [Journal Article] Mushroom body efferent neurons responsiblefor aversive olfactory memory retrieval in Drosophila (2011)
  • Author(s): Sejourne, J., Placais, P. Y., Aso, Y., Siwanowicz, I., Trannoy, S., Thoma, V., Tedjakumala, S. R., Rubin, G. M., Tchenio, P., Ito, K., Isabel, G., Tanimoto, H., Preat, T.
  • Journal Title: Nat Neurosci(14) Pages: 903-910
• [Journal Article] Flybrain neuron database : a comprehensive database system of the Drosophila brain neurons (2011)
  • Author(s): Shinomiya, K., Matsuda, K., Oishi, T., Otsuna, H., Ito, K.
  • Journal Title: J Comp Neurol Volume: 519 Issue: 5 Pages: 807-833
  • DOI: 10.1002/cne.22540
  • Peer Reviewed
• [Journal Article] Mushroom body efferent neurons responsible for aversive olfactory memory retrieval in Drosophila (2011)
  • Author(s): Sejourne, J., Placais, P.Y., Aso, Y., Siwanowicz, I., Trannoy, S., Thoma, V., Tedjakumala, S.R., Rubin, G.M., Tchenio, P., Ito, K., Isabel, G., Tanimoto, H., Preat, T.
  • Journal Title: Nat Neurosci Volume: 14 Issue: 7 Pages: 903-910
  • DOI: 10.1038/nn.2846
  • Peer Reviewed
• [Journal Article] Neural architectureof the primary gustatory center of Drosophilamelanogaster visualized with GAL4 and LexA enhancer-trap systems (2010)
  • Author(s): Miyazaki, T. and Ito, K.
  • Journal Title: J Comp Neurol(518) Pages: 4147-418
• [Journal Article] Specific dopaminergic neurons for the formation of labile aversive memory (2010)
  • Author(s): Aso, Y., Siwanowicz, I., Bracker, L., Ito, K., Kitamoto, T., and Tanimoto, H.
  • Journal Title: Curr Biol(20) Pages: 1445-1451
• [Journal Article] Neural architecture of the primary gustatory center of Drosophila melanogaster visualized with GAL4 and LexA enhancer-trap systems. (2010)
  • Author(s): Miyazaki, T., Ito, K.
  • Journal Title: J Comp Neurol Volume: 518 Pages: 4147-4181
  • Peer Reviewed
• [Journal Article] Specific dopaminergic neurons for the formation of labile aversive memory. (2010)
  • Author(s): Aso, Y., Siwanowicz, I., Bracker, L., Ito, K., Kitamoto, T., Tanimoto, H.
  • Journal Title: Cuff Biol Volume: 20 Pages: 1445-1451
  • Peer Reviewed
• [Journal Article] GABA-mediated neural connections in the Drosophila antennal lobe (2009)
  • Author(s): Okada, R., Awasaki, T. and Ito, K.
  • Journal Title: J Comp Neurol(514) Pages: 74-91
• [Journal Article] Odor-evoked neural oscillations in Drosophila are mediated by widely branching interneurons (2009)
  • Author(s): Tanaka, N. K., Ito, K. and Stopfer, M.
  • Journal Title: J Neurosci29 Pages: 8595-2603
• [Journal Article] GABA-mediated neural connections in the Drosophila antennal lobe (2009)
  • Author(s): Okada, R., Awasaki, T., Ito, K
  • Journal Title: J Comp Neurol 514 Pages: 74-91
  • Peer Reviewed
• [Journal Article] Odor-evoked neural oscillations in Drosophila are mediated by widely branching interneurons (2009)
  • Author(s): Tanaka, N.K., Ito, K., Stopfer, M
  • Journal Title: J Neurosci 29 Pages: 8595-603
  • Peer Reviewed
• [Presentation] Olfactory Receptor Neuron Identity is Diversified by the Drosophila Evi1/Prdm16 Homologue Hamlet that Mediates Chromatin Modification at Notch-Target Loci (2011)
  • Author(s): 遠藤啓太, MD Rezaul Karim, Alena Krejci, Emi Kinameri, Hiroaki Taniguchi, Matthias Siebert,伊藤啓, Sarah J. Bray, Adrian W. Moore
  • Organizer: 第44回発生生物学会大会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] Olfactory Receptor Neurons are Diversified by the Prdm Protein Hamlet that Mediates Chromatin Modification at Notch-Target Loci (2011)
  • Author(s): 遠藤啓太, MD Rezaul Karim, Alena Krejci, Emi Kinameri, Hiroaki Taniguchi, Matthias Siebert,伊藤啓, Sarah J. Bray, Adrian W. Moore
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: 台湾・台北・Chientan Youth Activity Center
• [Presentation] Projectome mapping of neural circuits in the Drosophila brain based on the neuroblast lineages (2011)
  • Author(s): 伊藤正芳,遠藤啓太,伊藤啓
  • Organizer: 第44回日本発生生物学会大会
  • Place of Presentation: 沖縄・沖縄コンベンションセンター
• [Presentation] Projectome Analysis of the Lineage-Dependent Neural Circuits in the Drosophila Brain (2011)
  • Author(s): 伊藤正芳,遠藤啓太,伊藤啓
  • Organizer: 1st Asia-Pacific Drosophila Research Conference
  • Place of Presentation: 台湾・台北・Chientan Youth Activity Center
• [Presentation] Projectome Mapping of the Lineage-Dependent Neural Circuits in the Drosophila Brain (2011)
  • Author(s): 伊藤正芳,遠藤啓太,伊藤啓
  • Organizer: 2011Cold Spring Harbor Laboratory Meeting on Neurobiology of Drosophila
  • Place of Presentation: 米・コールドスプリングハーバー
• [Presentation] Projectome Mapping of the Lineage-Dependent Neural Circuits in the Drosophila Brain (2011)
  • Author(s): Masayoshi Ito, Keita Endo, Kei Ito
  • Organizer: 2011 Cold Spring Harbor Laboratory Meeting on Neurobiology of Drosophila
  • Place of Presentation: 米・コールドスプリングハーバー
• [Presentation] Neural Structure of the Drosophila primary gustatory center revealed with GAL4 and LexA enhancer-trap systems (2010)
  • Author(s): 宮崎隆明、伊藤啓
  • Organizer: Functional Neurobiology in Minibrains : FromFlies to Robots and Back Again
  • Place of Presentation: スペイン・サン・フェリウ・デ・ギホルス
• [Presentation] Systematic analysis of the projection map of the primary gustatory center using two enhancer-trap systems in Drosophila melanogaster (2010)
  • Author(s): 宮崎隆明、伊藤啓
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台・東北大学川内キャンパス
• [Presentation] Comprehensive analysis of the neural network in Drosophila brain on the neuroblast lineages (2010)
  • Author(s): 伊藤正芳、遠藤啓太、伊藤啓
  • Organizer: 第43回日本発生生物学会年会
  • Place of Presentation: 京都・京都大学
• [Presentation] Understanding the brain composition from the viewpoint of cell lineage (2010)
  • Author(s): 伊藤啓、遠藤啓太、粟崎健、伊藤正芳
  • Organizer: 第43回日本発生生物学会年会
  • Place of Presentation: 京都・京都大学
• [Presentation] Neural Structure of the Drosophila primary gustatory center revealed with GAL4 and LexA enhancer-trap systems (2010)
  • Author(s): 宮崎隆明、伊藤啓
  • Organizer: Functional Neurobiology in Minibrains : From Flies to Robots and Back Again
  • Place of Presentation: スペイン・サン・フェリウ・デ・ギホルス
• [Presentation] Comprehensive analysis of the neural network in Drosophila brain on the neuroblast lineages (2010)
  • Author(s): 伊藤正芳、遠藤啓太、伊藤啓
  • Organizer: 第43回・本発生生物学会年会
  • Place of Presentation: 京都・京都大学
• [Presentation] Systematic mapping of the primary gustatory center in the Drosophila brain (2009)
  • Author(s): 伊藤啓,宮崎隆明
  • Organizer: European Society for Insect Taste and Olfaction Meeting
  • Place of Presentation: イタリア・サルジニア
  • Year and Date: 2009-09-23
• [Remarks]
  • URL: http://ndb.flybrain.org
• [Remarks]
  • URL: http://ndb.flybrain.org