New Strategy of Platelet Transfusion Independent of Donor Blood

• Project/Area Number: 21300160
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biomedical engineering/Biological material science
• Research Institution: Kyoto University (2011); The University of Tokyo (2009-2010)
• Principal Investigator: ETO Koji 京都大学, ips細胞研究所, 教授 (50286986)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2009: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 再生医工学 / 人工臓器工学 / iPS細胞 / 巨核球 / 血小板 / c-MYC / 細胞株 / 輸血 / 細胞老化 / メタロプロテアーゼ / 遺伝子 / デバイス / MEMS / 不死化細胞株

Research Abstract

This research program enabled to establish the methodology of induction of'megakaryocyte cell line' from human embryonic stem(ES) cells or induced pluripotent stem(iPS) cells, contributing to the huge amount of platelet generation in vitro. We also developed new drug to block the platelet inactivation occurred at 37°C through the inhibition of ADAM10 and ADAM17. The program of the development of new device for platelet production from megakaryocytes based upon'shear stress theory' enabled the

Research Products

• [Journal Article] In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cell (2012)
  • Author(s): Takayama N, Eto K
  • Journal Title: Methods Mol Biol Volume: 788 Pages: 205-17
• [Journal Article] Integrinαvβ3 regulates thrombopoietin-mediated maintenance of hematopoietic stem cells. Blood (2012)
  • Author(s): Umemoto T, Yamato M, Ishihara J, Shiratsuchi Y, Utsumi M, Morita Y, Tsukui H, Terasawa M, Shibata T, Nishida K, Kobayashi Y, Petrich BG, Nakauchi H, Eto K, Okano T.
  • Journal Title: Blood Volume: 119(1) Pages: 83-94
• [Journal Article] In vivo imaging visualizes discoid platelet aggregations without endothelium disruption and implicates contribution of inflammatory cytokine and integrin signaling (2012)
  • Author(s): Nishimura S, Manabe I, Nagasaki M, Kakuta S, Iwakura Y, Takayama N, Ooehara J, Otsu M, Kamiya A, Petrich B, Urano T, Kadono T, Sato S, Aiba A, Yamashita H, Sugiura S, Kadowaki T, Nakauchi H, Eto K, Nagai R.
  • Journal Title: Blood Volume: 119(8) Pages: 45-56
• [Journal Article] In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells (2012)
  • Author(s): Takayama N, Eto K
  • Journal Title: Methods Mol Biol Volume: 788 Pages: 205-217
  • DOI: 10.1007/978-1-61779-307-3_15
  • ISBN: 9781617793066, 9781617793073
  • Peer Reviewed
• [Journal Article] Integrin αvβ3 regulates thrombopoietin-mediated maintenance of hematopoietic stem cells (2012)
  • Author(s): Umemoto T, Yamato M, Ishihara J, Shiratsuchi Y, Utsumi M, Morita Y, Tsukui H, Terasawa M, Shibata T, Nishida K, Kobayashi Y, Petrich BG, Nakauchi H, Eto K, Okano T
  • Journal Title: Blood Volume: 119(1) Issue: 1 Pages: 83-94
  • DOI: 10.1182/blood-2011-02-335430
  • Peer Reviewed
• [Journal Article] ヒトiPS細胞からつくる血小板 (2012)
  • Author(s): 江藤浩之
  • Journal Title: Annual Review血液2012 Pages: 214-219
• [Journal Article] Heterozygous ITGA2B R995W mutation inducing constitutive activation of the{alpha} IIb{beta} 3 receptor affects proplatelet formation and causes congenital macro thrombocytopenia (2011)
  • Author(s): Kunishima S, Kashiwagi H, Otsu M, Takayama N, Eto K, Onodera M, Miyajima Y, Takamatsu Y, Suzumiya J, Matsubara K, Tomiyama Y, Saito H.
  • Journal Title: Blood Volume: 117 Pages: 5479-84
• [Journal Article] Potential usefulness of human iPS cells on the generation of platelets (2011)
  • Author(s): Takayama N, Eto K, Nakauchi H
  • Journal Title: Nihon Rinsho Volume: 69(12) Pages: 2161-2165
  • NAID: 40019080133
• [Journal Article] In vivo imaging visualizes discoid platelet aggregations without endothelium disruption and implicates contribution of inflammatory cytokine and integrin signaling (2011)
  • Author(s): S.Nishimura
  • Journal Title: Blood Volume: 119 Issue: 8 Pages: 128-131
  • DOI: 10.1182/blood-2011-09-381400
  • Peer Reviewed
• [Journal Article] Heterozygous ITGA2B R995W mutation inducing constitutive activation of the {alpha}IIb{beta}3 receptor affects proplatelet formation and causes congenital macrothrombocytopenia (2011)
  • Author(s): Kunishima S, Kashiwagi H, Otsu M, Takayama N, Eto K, Onodera M, Miyajima Y, Takamatsu Y, Suzumiya J, Matsubara K, Tomiyama Y, Saito H
  • Journal Title: Blood Volume: 117 Issue: 20 Pages: 5479-5484
  • DOI: 10.1182/blood-2010-12-323691
  • Peer Reviewed
• [Journal Article] ヒト多能性幹細胞を用いた巨核球/血小板造血研究 (2011)
  • Author(s): 高山直也、江藤浩之
  • Journal Title: 医学のあゆみ Volume: 239 Pages: 1385-1389
• [Journal Article] iPS細胞樹立法の標準化を目指した分化能による評価 (2011)
  • Author(s): 遠藤大、江藤浩之
  • Journal Title: 細胞 Volume: 43 Pages: 367-370
• [Journal Article] 挑戦することを教えてくれたメンターたちとの出会い (2011)
  • Author(s): 江藤浩之
  • Journal Title: 日本血栓止血学会誌 Volume: 22 Pages: 291-294
  • NAID: 10031162956
• [Journal Article] 血小板と再生医学 (2011)
  • Author(s): 江藤浩之、遠藤大
  • Journal Title: 脈管学 Volume: 51 Pages: 339-345
  • NAID: 10029767563
• [Journal Article] Reduction of N-Glycolylneuraminic Acid in Human Induced Pluripotent Stem Cells Generated or Cultured under Feeder-and Serum-Free Defined Conditions (2010)
  • Author(s): Hayashi Y, Chan T, Warashina M, Fukuda M, Ariizumi T, Okabayashi K, Takayama N, Otsu M, Eto K, Furue MK, Michiue T, Ohnuma K, Nakauchi H, Asashima M.
  • Journal Title: Plops One Volume: 5 Pages: 14099-14099
• [Journal Article] Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells (2010)
  • Author(s): Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H, Yamaguchi T, Otsu M, Nishimura K, Nakanishi M, Sawaguchi A, Nagai R, Takahashi K, Yamanaka S, Nakauchi H, Eto K.
  • Journal Title: J Exp Med Volume: 207 Pages: 2817-30
• [Journal Article] Essential in vivo roles of the c-type lectin receptor CLEC-2 : Embryonic/neonatal lethality of CLEC-2-deficient mice by blood/lymphatic misconnections and impaired thrombus formation of CLEC-2-deficient platelets (2010)
  • Author(s): Suzuki-Inoue K, Inoue O, Ding G, Nishimura S, Hokamura K, Eto K, Kashiwagi H, Tomiyama Y, Yatomi Y, Umemura K, Shin Y, Hirashima M, Ozaki Y.
  • Journal Title: J Biol Chem Volume: 285 Pages: 24494-507
• [Journal Article] Lnk regulates integrin alphaIIbbeta3 outside-in signaling in mouse platelets, leading to stabilization of thrombus development in vivo (2010)
  • Author(s): Takizawa H, Nishimura S, Takayama N, Oda A, Nishikii H, Morita Y, Kakinuma S, Yamazaki S, Okamura S, Tamura N, Goto S, Sawaguchi A, Manabe I, Takatsu K, Nakauchi H, Takaki S, Eto K.
  • Journal Title: J Clin Invest Volume: 120 Pages: 179-90
• [Journal Article] Lnk regulates integrin alphaIIbbeta3 outside-in signaling in mouse platelets, leading to stabilization of thrombus development in vivo. (2010)
  • Author(s): Takizawa H, Nishimura S, Takayama N, Oda A, Nishikii H, Morita Y, Kakinuma S, Yamazaki S, Okamura S, Tamura N, Goto S, Sawaguchi A, Manabe I, Takatsu K, Nakauchi H, Takaki S, Eto K.
  • Journal Title: Journal of Clinical Investigation Volume: 120 Pages: 179-190
  • Peer Reviewed
• [Journal Article] Essential in vivo roles of the c-type lectin receptor CLEC-2: Embryonic/neonatal lethality of CLEC-2-deficient mice by blood/lymphatic misconnections and impaired thrombus formation of CLEC-2-deficient platelets. (2010)
  • Author(s): Suzuki-Inoue K, Inoue O, Ding G, Nishimura S, Hokamura K, Eto K, Kashiwagi H, Tomiyama Y, Yatomi Y, Umemura K, Shin Y, Hirashima M, Ozaki Y.
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 24494-24507
  • Peer Reviewed
• [Journal Article] Reduction of N-Glycolylneuraminic Acid in Human Induced Pluripotent Stem Cells Generated or Cultured under Feeder-and Seru m-Free Defined Conditions. (2010)
  • Author(s): Hayashi Y, Chan T, Warashina M, Fukuda M, Ariizumi T, O kabayashi K, Takayama N, Otsu M, Eto K, Furue MK, Michiue T, Ohnuma K, Nakauchi H, Asashima M
  • Journal Title: PLoS One Volume: 15
  • Peer Reviewed
• [Journal Article] Transient activation of c-MYC expression is critical for efficient platelet generation from human induced pluripotent stem cells. (2010)
  • Author(s): Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H, Yamaguchi T, Otsu M, Nishimura K, Nakanishi M, Sawaguchi A, Nagai R, Takahashi K, Yamanaka S, Nakauchi H, Eto K
  • Journal Title: Journal of Experimental Medicine Volume: 207 Pages: 2817-2830
  • Peer Reviewed
• [Journal Article] Lnk/Sh2b3 regulates integrin αIIbβ3 outside-in signaling in mouse platelets leading to stabilization of developing thrombus in vivo (2010)
  • Author(s): Takizawa H, Nishimura S, Takayama N, Oda A, Nishikii H, Morita Y, Kakinuma S, Yamazaki S, Okamura S, Tamura N, Goto S, Sawaguchi A, Manabe I, Takatsu K, Nakauchi H, Takaki S, Eto K
  • Journal Title: Journal of Clinical Investigation 120 Pages: 179-190
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): 江藤浩之、高山直也、西村智
  • Journal Title: iPS細胞からの血小板への分化誘導と新しい輸血システムの開発【実験医学増刊 再生医療の最前線2010「第3章 幹細胞の分化誘導と臨床応用」】(羊土社) Pages: 217-222
• [Journal Article] Evidence that integrin alpha IIb beta 3-dependent interaction of mast cells with fibrinogen exacerbates chronic inflammation (2009)
  • Author(s): Oki T, Eto K, Izawa K, Yamanishi Y, Inagaki N, Frampton J, Kitamura T, Kitaura J.
  • Journal Title: J Biol Chem Volume: 284 Pages: 31463-72
• [Journal Article] Definitive proof for direct reprogramming of hematopoietic cells to pluripotency (2009)
  • Author(s): Okabe M, Otsu M, Ahn DH, Kobayashi T, Wakiyama Y, Morita Y, Onodera M, Eto K, Ema H, Nakauchi H
  • Journal Title: Blood Volume: 114 Pages: 1764-7
• [Journal Article] CD8+ effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity (2009)
  • Author(s): Nishimura S, Manabe I, Nagasaki M, Eto K, Yamashita H, Ohsugi M, Otsu M, Hara K, Ueki K, Sugiura S, Yoshimura K, Kadowaki T, Nagai R.
  • Journal Title: Nat Med Volume: 15 Pages: 914-20
• [Journal Article] The actin polymerization regulator WAVE2 is required for early bone marrow repopulation by hematopoietic stem cells (2009)
  • Author(s): Ogaeri T, Eto K, Otsu M, Ema H, Nakauchi H.
  • Journal Title: Stem Cells Volume: 27 Pages: 1120-9
• [Journal Article] TGF-β induces hibernation of hematopoietic stem cells in the bone marrow niche (2009)
  • Author(s): Yamazaki S, Iwama A, Takayanagi S, Eto K, Ema H, Nakauchi H
  • Journal Title: Blood 113 Pages: 1250-1256
  • Peer Reviewed
• [Journal Article] Stepwise development of hematopoietic stem cells from embryonic stem cells (2009)
  • Author(s): Matsumoto K, Isagawa T, Nishimura T, Ogaeri T, Eto K, Miyzaki S, Miyazaki JI, Aburatani H, Nakauchi H, Ema H
  • Journal Title: PLosONE 4
  • NAID: 120004898447
  • Peer Reviewed
• [Journal Article] The actin polymerization regulator WAVE2 is required for early bone marrow repopulation by hematopoietic stem cells (2009)
  • Author(s): Ogaeri T, Eto K, Otsu M, Ema H, Nakauchi H
  • Journal Title: Stem Cells 27 Pages: 1120-1129
  • Peer Reviewed
• [Journal Article] Definitive proof for direct reprogramming of hematopoietic cells to pluripotency (2009)
  • Author(s): Okabe M, Otsu M, Ahn DH, Kobayashi T, Morita Y, Wakiyama Y, Onodera M, Eto K, Ema H, Nakauchi H
  • Journal Title: Blood 114 Pages: 1764-1767
  • Peer Reviewed
• [Journal Article] CD8+ effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity (2009)
  • Author(s): Nishimura S, Manabe I, Nagasaki M, Eto K, Yamashita H, Ohsugi M, Otsu M, Hara K, Sugiura S, Yoshimura K, Kadowaki T, Nagai R
  • Journal Title: Nature Medicine 15 Pages: 914-920
  • Peer Reviewed
• [Journal Article] Evidence that integrin αIIbβ3-dependent interaction of mast cells with fibrinogen exacerebrates chronic inflammation (2009)
  • Author(s): Oki T, Eto K, Izawa K, Yamanishi Y, Inagaki N, Frampton J, Kitamura T, Kitaura J
  • Journal Title: Journal of Biological Chemistry 284 Pages: 31463-31472
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 江藤浩之
  • Journal Title: ES・iPS細胞の有用性に着目した血小板輸血法の開発-献血のいらない血小板製剤への挑戦(医学のあゆみ) Pages: 495-496
• [Journal Article] (2009)
  • Author(s): 江藤浩之
  • Journal Title: ES細胞およびiPS細胞からの血小板産生【出血性疾患:診断と治療の進歩】(日本内科学会雑誌) Pages: 119-126
• [Journal Article] (2009)
  • Author(s): 江藤浩之、中内啓光
  • Journal Title: ES細胞由来血小板【治療学「再生医療の細胞ソース3」】(ライフサイエンス社) Pages: 609-614
• [Journal Article] (2009)
  • Author(s): 江藤浩之、高山直也、中内啓光
  • Journal Title: ES細胞・iPS細胞からの血液細胞の分化誘導-献血に頼らない血小板輸血の実現-【幹細胞の分化誘導と応用「第4編 幹細胞の分化誘導と移植技術」】(NTS社) Pages: 159-167
• [Journal Article] (2009)
  • Author(s): 江藤浩之、西村智
  • Journal Title: iPS細胞からの血小板産生技術とその方向性【血液フロンティア「再生医療の現状と進歩」】(医薬ジャーナル社) Pages: 1693-1699
• [Journal Article] (2009)
  • Author(s): 江藤浩之、高山直也、中内啓光
  • Journal Title: iPS細胞からの血小板産生【別冊新しい診断と治療のABC「血小板減少症・増加症」】(最新医学社) Pages: 182-189
• [Presentation] ES Cells and iPS Cells for Cardiovascular Research (2012)
  • Author(s): Eto K, Gary Lyons, Andre Terzic.
  • Organizer: American Heart Association SCIENTIFIC SESSIONS 2010
  • Place of Presentation: (Chicago, USA) Invited Speaker(Session)
• [Presentation] まれな血液型およびHLAタイプ患者のためのiPS細胞技術を用いた新しい輸血システム開発 (2011)
  • Author(s): 江藤浩之
  • Organizer: 第18回日本血液代替物学会年次大会
  • Place of Presentation: 札幌
  • Year and Date: 2011-10-27
• [Presentation] Blood generation from iPS cells toward clinical applications (2011)
  • Author(s): Eto K.
  • Organizer: 第32回日本炎症・再生医学会1st Meeting of Asian-Pacific Federation of Inflammation and Regeneration合同開催
  • Place of Presentation: (Kyoto, JAPAN.) Invited Speaker
• [Presentation] 重症先天性好中球減少症患者由来のiPS細胞の樹立とその解析 (2011)
  • Author(s): 平本貴史、海老原康博、馬峰、望月慎史、西濱夏海、花田佐智代、松坂恵美子、江藤浩之、中内啓光、辻浩一郎
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都(ポスター)
• [Presentation] 輸血医療革命をもたらす多能性幹細胞由来不死化巨核球細胞株樹立技術の開発 (2011)
  • Author(s): 中村壮、高山直也、中内啓光、江藤浩之
  • Organizer: 第32回日本炎症・再生医学会
  • Place of Presentation: 京都(ポスター)
• [Presentation] iPS細胞バンクを有効利用した血液疾患治療法の開発 (2011)
  • Author(s): 江藤浩之
  • Organizer: 第24回日本動物細胞工学会2011年度大会
  • Place of Presentation: 東京(招待講演)
• [Presentation] Platelet transfusion system using an immortalized megakaryocyte cell line derived from hESCs/iPSCs (2011)
  • Author(s): Sou Nakamura, Naoya Takayama, Hiromitsu Nakauchi, Koji Eto
  • Organizer: 第73回日本血液学会学術集会
  • Place of Presentation: 名古屋
• [Presentation] Blood generation from iPS cells toward clinical applications (2011)
  • Author(s): Eto K
  • Organizer: 第32回日本炎症・再生医学会1st Meeting of Asian-Pacific Federation of Inflammation and Regeneration合同開催
  • Place of Presentation: Kyoto, JAPAN(Invited)
• [Presentation] SUSPECTED INCOMPLETE REPROGRAMMING IN BLOOD DERIVED HUMAN INDUCED PLUR IPOTENT STEM CELLS IS ADVANTAGEOUS FOR ADULT-TYPE ERYTHROCYTE GENERATI ON WITH GLOBIN SWITCHING (2011)
  • Author(s): Takayama N, Sano S, Shimizu T, Kawahata R, Endo H, Nakamura S, Ogawa S, Nakauchi H, Eto K
  • Organizer: International Society for Stem Cell Research 9th Annual Meeting
  • Place of Presentation: Toronto, Canada(poster)
• [Presentation] Potential application of human iPS cell-derived hematopoietic cells for disease treatment (2011)
  • Author(s): Eto K
  • Organizer: 7th IABS Symposium on Advances in Transfusion Safety
  • Place of Presentation: Singapore(Invited)
• [Presentation] TRANSIENT ACTIVATION OF C-MYC EXPRESSION IS CRITICAL FOR EFFICIENT PLA TELET GENERATION FROM HUMAN INDUCED PLURIPOTENT STEM CELLS (2011)
  • Author(s): Takayama N, Nishimura S, Nakamura S, Shimizu T, Ohnishi R, Endo H, Yamaguchi T, Otsu M, Nishimura K, Nakanishi M, Sawaguchi A, Nagai R, Takahashi K, Yamanaka S, Nakauchi H, Eto K
  • Organizer: XXIII Congress of the International Society on Thrombosis and Haemostasis
  • Place of Presentation: Kyoto, JAPAN(poster)
• [Presentation] Potential application of human iPS cell-derived blood cells and evaluation system in mouse xenogeneic transplantation models (2011)
  • Author(s): Eto K
  • Organizer: The Third International Workshop on Humanized Mice
  • Place of Presentation: Pittsburgh, USA(Invited)
• [Presentation] PRODUCTION OF PLATELETS FROM STEM CELLS (2011)
  • Author(s): Eto K
  • Organizer: The XXIInd Regional Congress of the ISBT
  • Place of Presentation: Taipei(Invited)
• [Presentation] Platelet Production System Using an Immortalized Megakaryocyte Cell Line Derived From Human Pluripotent Stem Cells (2011)
  • Author(s): Sou Nakamura, Naoya Takayama,, Hiromitsu Nakauchi, Koji Eto
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(plenary session)
• [Presentation] Platelet Production System Using an Immortalized Megakaryocyte Cell Line Derived From Human Pluripotent Stem Cells (2011)
  • Author(s): Sou Nakamura, Naoya Takayama,, Hiromitsu Nakauchi, Koji Eto
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(Invited)
• [Presentation] Modeling Congenital Amegakaryocytic Thrombocytopenia Using Patient-Specific Induced Pluripotent Stem Cells (2011)
  • Author(s): Naoya Takayama, Shinji Hirata, Ryoko Jono-Ohnishi, Sou Nakamura, Sho-ichi Hirose, Hiroshi Endo, Masanori Nishi, Yuhei Hamazaki, Shin Kaneko, Eiichi Ishii, Hiromitsu Nakauchi, Shinji Kunishima, Koji Eto
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(oral)
• [Presentation] Suppressed Neutrophil Development in Hematopoiesis of Induced Pluripotent Stem Cells Derived From a Severe Congenital Neutropenia Patient with ELA2 Mutation (2011)
  • Author(s): Takafumi Hiramoto, Yasuhiro Ebihara, Yoko Mizoguchi, Kazuhiro Nakamura, Shinji Mochizuki, Shohei Yamamoto, Emiko Matsuzaka, Sachiyo Hanada, Ryoko Ohnishi, Kenzaburo Tani, Koji Eto, Hiromitsu Nakauchi, Masao Kobayashi, Kohichiro Tsuji
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(oral)
• [Presentation] Nov/CCN3 Enhances Long-Term Repopulating Activity of Mouse Hematopoietic Stem Cells Via Intergin β3 Signaling Collaborating with Thrombopoietin (2011)
  • Author(s): Jun Ishihara, Terumasa Umemoto, Masayuki Yamato, Yoshiko Shiratsuchi, Brian G.Petrich, Hiromitsu Nakauchi, Koji Eto, Toshio Kitamura, Teruo Okano
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(oral)
• [Presentation] Direct and Continuous Inhibition of ADAM17 Using a Novel Selective Inhibitor Restores Functional Platelet Yield From Human Pluripotent Stem Cells (2011)
  • Author(s): Shinji Hirata, Ryoko Jono-Ohnishi, Satoshi Nishimura, Naoya Takayama, Sou Nakamura, Hiromitsu Nakauchi, Takahiko Murata, Koji Eto
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(poster)
• [Presentation] Stepwise Signaling and Low Oxygen Promote Hematopoietic Progenitor Emergence From Human Pluripotent Stem Cells (2011)
  • Author(s): Hiroshi Endo, Naoya Takayama, Tomo Koike, Hiromitsu Nakauchi, Koji Eto
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(poster)
• [Presentation] In Vitro Generation of Mature T Lymphocytes From Human Ips Cells and Genetic Analysis of TCR Gene Rearrangements (2011)
  • Author(s): Toshinobu Nishimura, Shin Kaneko, Yoko Tajima, Naoya Takayama, Ai Tachikawa-Kawana, Koji Eto, Hiromitsu Nakauchi
  • Organizer: 53rd ASH Annual Meeting and Exposition
  • Place of Presentation: San Diego, USA(poster)
• [Presentation] ES Cells and iPS Cells for Cardiovascular Research (2010)
  • Author(s): Eto K, Gary Lyons, Andre Terzic
  • Organizer: American Heart Association Scientific Session Annual Meeting 2010
  • Place of Presentation: Chicago, USA
  • Year and Date: 2010-12-14
• [Presentation] Generation of Monoclonal TCR-Expressing Human T-Lineage Lymphocytes From Induced Pluripotent Stem Cells of Signal Peripheral T-Ly mphocyte Origin (2010)
  • Author(s): Nishimura T, Kaneko S, Gotoh H, Takayama N, Shimizu T, Iriguchi S, Tajima Y, Yasui Y, Watanabe N, Takahashi S, Eto K, Nakauchi H
  • Organizer: 52st American Society of Hematology Annual Meeting
  • Place of Presentation: Orlando, U.S.A
  • Year and Date: 2010-12-06
• [Presentation] Epigenetic Memory Enables the Dominant Generation of Adult-Type Erythrocytes From Human Induced Pluripotent Stem Cells (2010)
  • Author(s): Takayama N, Sano S, Shimizu T, Kawahata R, Endo H, Nakamura S, Ogawa S, Nakauchi H, Eto K
  • Organizer: 52st American Society of Hematology Annual Meeting
  • Place of Presentation: Orlando, U.S.A
  • Year and Date: 2010-12-06
• [Presentation] In vivo imaging reveals multi-cellular kinetics in pathological conditions including metabolic syndrome and thrombus formation (2010)
  • Author(s): Nishimura S, Nagasaki M, Manabe I, Eto K, Kadowaki T, Nagai R.
  • Organizer: 第71回日本血液学会学術総会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-10-24
• [Presentation] c-MYC再活性化に伴うヒトiPS細胞からの効率の良い巨核球/血小板産生法の確立 (2010)
  • Author(s): 高山直也、中村壮、大西椋子、澤口朗、高橋和利、山中伸弥、江藤浩之、中内啓光
  • Organizer: 第71回日本血液学会学術総会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2010-10-24
• [Presentation] Novel Target of Ex Vivo Expansion; Integrin avβ3 Ligation Enables Regulation of Hematopoietic Stem Cell Division Leading to Maintenance of Reconstitution Potential (2010)
  • Author(s): Koji Eto
  • Organizer: The 1st ISCT Asia-Pacific Regional Meeting
  • Place of Presentation: Miyazaki, Japan 宮崎県フェニクスシーガイアリゾート サミットホール
  • Year and Date: 2010-10-19
• [Presentation] Human Induced Pluripotent Stem Cell-derived Blood Cells toward Clinical Application (2010)
  • Author(s): Koji Eto
  • Organizer: The 1st ISCT Asia-Pacific Regional Meeting
  • Place of Presentation: 宮崎県フェニクスシーガイアリゾート サミットホール
  • Year and Date: 2010-10-18
• [Presentation] R995W mutation in integrin α IIb-gene (ITGA2B) is a novel cause for congenital macrothrombocytopenia (2010)
  • Author(s): Kousaku Matsubara, Shinji Kunishima, Toshiro Takafuta, Yoshiko Uchida, Takashi Fukakya, Daichi Inoue, Hiroakazu Kashiwagi, Yoshiaki Tomiyama, Makoto Otsu, Koji Eto, Masafumi Onodera
  • Organizer: 第72回日本血液学会学術集会
  • Place of Presentation: 横浜 パシフィコ横浜
  • Year and Date: 2010-09-26
• [Presentation] Human iPS cells from Blood and Blood from iPS cells (2010)
  • Author(s): Koji Eto
  • Organizer: Hematopoiesis in Health and Disease in Lund
  • Place of Presentation: Lund, Sweden
  • Year and Date: 2010-05-17
• [Presentation] Lnk/Sh2b3による血小板シグナルおよび線溶系制御を介した止血血栓制御機構 (2010)
  • Author(s): 江藤浩之、西村智、真鍋一郎、柿沼晴、小田淳、永井良三、中内啓光、高木智.
  • Organizer: 第33回日本血栓止血学会学術集会
  • Place of Presentation: 鹿児島.城山観光ホテル
  • Year and Date: 2010-04-24
• [Presentation] 巨核球造血,血小板産生異常を呈する病態解析細胞モデルの開発 (2010)
  • Author(s): 大西椋子、高山直也、大津真、西村智、西範、浜崎雄平、石井榮一、小田淳、國島伸治、中内啓光、江藤浩之
  • Organizer: 第33回日本血栓止血学会学術集会
  • Place of Presentation: 鹿児島.城山観光ホテル
  • Year and Date: 2010-04-23
• [Presentation] iPS細胞の基礎知識:What iPS Cells Gave Us? (2010)
  • Author(s): 江藤浩之
  • Organizer: 日本循環器学会総会モーニングレクチャー
  • Place of Presentation: 京都
  • Year and Date: 2010-03-06
• [Presentation] iPS細胞の臨床応用とその課題:Introduction of the Project at the University of Tokyo～development of next generation of stem cell-based gene- and cellular therapy (2010)
  • Author(s): 江藤浩之
  • Organizer: 日本循環器学会総会シンポジウム(招待講演)
  • Place of Presentation: 京都
  • Year and Date: 2010-03-06
• [Presentation] Epigenetic Memory Enables the Dominant Generation of Adult-Type Erythrocytes From Human Induced Pluripotent Stem Cells (2010)
  • Author(s): Takayama N, Sano S, Shimizu T, Kawahata R, Endo H, Nakamura S, Ogawa S, Nakauchi H, Eto K.
  • Organizer: 52st American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: (Orlando, USA) Oral
• [Presentation] Generation of Monoclonal TCR-Expressing Human T-Lineage Lymphocytes From Induced Pluripotent Stem Cells of Signal Peripheral T-Lymphocyte Origin (2010)
  • Author(s): Nishimura T, Kaneko S, Gotoh H, Takayama N, Shimizu T, Iriguchi S, Tajima Y, Yasui Y, Watanabe N, Takahashi S, Eto K, Nakauchi H.
  • Organizer: 52st American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: (Orlando, USA) Oral
• [Presentation] Novel Target of Ex Vivo Expansion ; Integrinαvβ3 Ligation Enables Regulation of Hematopoietic Stem Cell Division Leading to Maintenance of Reconstitution Potential (2010)
  • Author(s): Eto K.
  • Organizer: The 1st ISCT Asia-Pacific Regional Meeting
  • Place of Presentation: (Miyazaki, Japan.) Invited Speaker
• [Presentation] Human Induced Pluripotent Stem Cell-derived Blood Cells toward Clinical Application (2010)
  • Author(s): Eto K.
  • Organizer: The 1st ISCT Asia-Pacific Regional Meeting
  • Place of Presentation: (Miyazaki, Japan.) Invited Speaker
• [Presentation] Generation of engraft able hematopoietic stem cells from teratomas formed by an injection of induced pluripotent stem cells (2010)
  • Author(s): Suzuki N, Yamazaki S, Yamaguchi T, Takaki S, Eto K, Nakauchi H.
  • Organizer: International Society for Stem Cell Research 8th Annual Meeting(ISSCR)
  • Place of Presentation: (San Francisco, USA) Oral Session
• [Presentation] Feeder and serum free culture of human induced pluripotent stem cells (2010)
  • Author(s): Chan T, Hayashi Y, Takayama N, Otsu M, Eto K, Furue-Kusuda M, Ohnuma K, Nakauchi H, Asashima M.
  • Organizer: International Society for Stem Cell Research 8th Annual Meeting(ISSCR)
  • Place of Presentation: (San Francisco, USA) Poster Session
• [Presentation] Monoclonal guidance of T Lineage lymphocytes from human induced pluripotent stem cells originating from a single peripheral T Lymphocyte (2010)
  • Author(s): Nishimura T, Kaneko S, Gotoh H, Takayama N, Nakamura S, Shimizu T, Iriguchi S, Tachikawa-Kawana A, Takahashi S, Watanabe N, Otsu M, Eto K, Nakauchi H.
  • Organizer: International Society for Stem Cell Research 8th Annual Meeting(ISSCR)
  • Place of Presentation: (San Francisco, USA) Poster Session
• [Presentation] CD61 is involved in thrombopoietin-mediated maintenance of mouse hematopoietic stem cells (2010)
  • Author(s): Umemoto T, Yamato M, Shiratsuchi Y, Utsumi M, Terasawa M, Morita Y, Nishida K, Kobayashi Y, Petrich Brian G, Ginsberg Mark H, Nakauchi H, Eto K, Okano T.
  • Organizer: International Society for Stem Cell Research 8th Annual Meeting(ISSCR)
  • Place of Presentation: (San Francisco, USA) Poster Session
• [Presentation] Novel in vivo imaging enables single-cell-level evaluation of the functionality of platelets derived from human induced pluripotent stem cells (2010)
  • Author(s): Nishimura S, Takayama N, Otsu M, Nakamura S, Ohnishi R, Endo H, Nagai R, Nakauchi H, Eto K.
  • Organizer: International Society for Stem Cell Research 8th Annual Meeting(ISSCR)
  • Place of Presentation: (San Francisco, USA) Poster Session
• [Presentation] Human iPS cells from Blood and Blood from iPS cells (2010)
  • Author(s): Eto K.
  • Organizer: Hematopoiesis in Health and Disease in Lund
  • Place of Presentation: (Lund, Sweden.) Invited Speaker
• [Presentation] 東京大学ips細胞等研究拠点における疾患特異的ips細胞の樹立 (2010)
  • Author(s): 大西椋子, 高山直也、大津真、安東赫、中村壮、遠藤大、西眞範、浜崎雄平、石井榮一、有賀正、國島伸治、中内啓光、江藤浩之
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
• [Presentation] 血液細胞由来PS細胞の機能的'記憶' (2010)
  • Author(s): 高山直也、中村壮、遠藤大、大西椋子, 工藤都湖、安東赫、大津真、江藤浩之、中内啓光
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
• [Presentation] ヒト人工多能性幹細胞(iPSCs)からのT細胞誘導の試み (2010)
  • Author(s): 後藤晴雄、金子新、高山直也、西村聡修、清水崇史、渡部信和、江藤浩之、中内啓光
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
• [Presentation] ヒト末梢血T細胞からの人工多能性幹細胞(iPSCs)の誘導 (2010)
  • Author(s): 西村聡修、金子新、後藤晴雄、高山直也、中村壮、立川愛、入口翔一、岩本愛吉、渡部信和、江藤浩之、中内啓光
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
• [Presentation] 造血幹細胞におけるCD61/integrin β3の役割 (2010)
  • Author(s): 白土佳子、梅本晃正、大和雅之、塚田健二、寺沢公男、柴田岳彦、西田幸二、Brian Petrich, Mark H.Ginsberg, 小林芳郎、中内啓光、江藤浩之、岡野光夫
  • Organizer: 第9回日本再生医療学会総会
  • Place of Presentation: 広島
• [Presentation] Novel therapeutic strategy in patients who require repeated transfusion of human lukocyte antigen-matched platelets derived from human induced plurinotent stem cells (2009)
  • Author(s): Koji Eto
  • Organizer: 国際輸血学会シンポジウム(招待講演)
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-17
• [Presentation] ES・iPS細胞からの血小板産生 (2009)
  • Author(s): 江藤浩之
  • Organizer: 日本輸血細胞治療学会シンポジウム(招待講演)
  • Place of Presentation: 大宮
  • Year and Date: 2009-05-28
• [Presentation] Cancellation of c-MYC Silencing in Human Induced Pluripotent Stem Cells Contributes to the Efficient in Vitro Production of Platelets with the Ability of Hemostasis In Vino (2009)
  • Author(s): Takayama N, Nakamura S, Nishimura S, Ohnishi R, Takahashi K, Yamanaka S, Eto K, and Nakauchi H.
  • Organizer: 51st American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: (New Orleans, USA) Poster Session
• [Presentation] CD61/Integrinβ3 Ligation Contributes to the Thrombopoietin-Mediated Niche Function of Mouse Hematopoietic Stem Cells (2009)
  • Author(s): Umemoto T, Yamato M, Shiratsuchi Y, Tsukada K, Utsumi M, Morita Y, Terasawa M, Shibata T, Nishida K, Kobayashi Y, Petrich Brian G, Ginsberg Mark H, Nakauchi H, Eto K, and Okano T.
  • Organizer: 51st American Society of Hematology Annual Meeting and Exposition
  • Place of Presentation: (New Orleans, USA) Speaker
• [Presentation] NOVEL THERAPEUTIC STRATEGY IN PATIENTS WHO REQUIRE REPEATED TRANSFUSION OF HUMAN LEUKOCYTE ANTIGEN-MATCHED PLATELETS DERIVED FROM HUMAN INDUCED PLURIPOTENT STEM CELLS (2009)
  • Author(s): Eto K.
  • Organizer: XXth REGIONAL CONGRESS, ASIA
  • Place of Presentation: (Nagoya, JAPAN.) Invited Speaker
• [Presentation] NOVEL THERAPEUTIC STRATEGY IN PATIENTS WHO REQUIRE REPEATED TRANSFUSION OF HUMAN LEUKOCYTE ANTIGEN-MATCHED PLATELETS DERIVED FROM HUMAN IPS CELLS (2009)
  • Author(s): Takayama N, Eto K, Nakamura S, Ohnishi R, Yamanaka S, Nakauchi H.
  • Organizer: International Society for Stem Cell Research 7th Annual Meeting(ISSCR)
  • Place of Presentation: (Barce Session lona, Spain.) Poster Seccion
• [Presentation] c-MYC再活性化に伴うヒトiPS細胞からの効率の良い巨核球/血小板産生法の確立 (2009)
  • Author(s): 高山直也、中村壮、大西椋子、澤口朗、高橋和利、山中伸哉、江藤浩之、中内啓光
  • Organizer: 第71回日本血液学会総会(一般講演)
  • Place of Presentation: 京都
• [Presentation] In vivo imaging reveals multi-cellular kinetics in pathological conditions including metabolic syndrome and thrombus formation (2009)
  • Author(s): Satoshi Nishimura、Mika Nagasaki、Ichiro Manabe、Koji Eto、Takashi Kadowaki、Ryozo Nagai
  • Organizer: 第71回日本血液学会総会(シンポジウム)
  • Place of Presentation: 京都
• [Presentation] 成体マウス骨髄血球細胞からのiPS細胞の樹立 (2009)
  • Author(s): 岡部基人、大津真、安東赫、小林俊寛、守田陽平、脇山由起子、江藤浩之、依馬秀夫、中内啓光
  • Organizer: 第71回日本血液学会総会(一般ポスター)
  • Place of Presentation: 京都
• [Presentation] Novel Therapeutic Strategy in Patients who Require Repeated Transfusion of Human Leukocyte Antigen-Matched Platelets Derived from Human iPS Cells (2009)
  • Author(s): Takayama N, Eto K, Nakamura S, Ohnishi R, Yamanaka S, Nakauchi H
  • Organizer: International Society for Stem Cell Research 7th Annu ISSCR 7^<th> annual Meeting(poster)
  • Place of Presentation: Barcelona, Spain
• [Presentation] Definitive Proof for Direct Reprogramming of Hematopoietic Cells to Pluripotency (2009)
  • Author(s): Okabe M, Otsu M, Ahn D H, Kobayashi T, Morita Y, Wakiyama Y, Onodera M, Eto K, Ema H, Nakauchi H
  • Organizer: International Society for Stem Cell Research 7th Annu ISSCR 7^<th> annual Meeting(poster)
  • Place of Presentation: Barcelona, Spain
• [Presentation] Cancellation of c-MYC silencing in human induced pluripotent stem cells contributes to the efficient in vitro production of platelets with the ability of hemostasis in vivo (2009)
  • Author(s): Takayama N, Nakamura S, Nishimura S, Ohnishi R, Takahashi K, Yamanaka S, Eto K, Nakauchi H
  • Organizer: International Society for Stem Cell Research 7th Annu 51^<th> American Society of Hematology(poster).
  • Place of Presentation: New Orleans(USA)
• [Presentation] CD61/Integrin β3 Ligation Contributes to the Thrombopoietin-Mediated Niche Function of Mouse Hematopoietic Stem Cells (2009)
  • Author(s): Umemoto T, Yamato M, Shiratsune Y, Tsukada K, Utsumi M, Morita Y, Terasawa M, Shibata T, Nishida K, Kobayashi Y, Petrich GB, Ginsberg MH, Nakauchi H, Eto K, Okano T
  • Organizer: International Society for Stem Cell Research 7th Annu 51^<th> American Society of Hematology(oral).
  • Place of Presentation: New Orleans(USA)
• [Book] ips細胞からの血小板への分化誘導と新しい輸血システムの開発 (2010)
  • Author(s): 江藤浩之、高山直也、西村智
  • Publisher: 実験医学
• [Book] 治療学特集「抗血栓療法適切な治療戦略の選択とその評価」基礎的領域から (2010)
  • Author(s): 江藤浩之、高山直也、西村智
  • Publisher: ライフサイエンス出版
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/j/research/eto_summary.html
• [Remarks]
  • URL: http://www.cira.kyoto-u.ac.jp/eto/
• [Remarks] CiRA HP
  • URL: http://www.cira.kyoto-u.ac.jp/j/research/eto_summary.html
• [Remarks] 江藤研究室HP
  • URL: http://www.cira.kyoto-u.ac.jp/eto/
• [Patent(Industrial Property Rights)] 多能性幹細胞からの巨血球及び/又は血小板の製造方法 (2011)
  • Inventor(s): 西野泰斗、中村隆典、岩本俊介、江藤浩之、辻嘉代子、中内啓光
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Number: 2011-108253
  • Filing Date: 2011-05-13
  • Overseas
• [Patent(Industrial Property Rights)] 血小板の機能を維持するための組成物 (2011)
  • Inventor(s): 江藤浩之、大西椋子、中内啓光、村田隆彦
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Filing Date: 2011-09-16
  • Overseas
• [Patent(Industrial Property Rights)] 多能性幹細胞からの血液細胞分化に関する培養方法 (2011)
  • Inventor(s): 佐野進弥、江藤浩之、高山直也、中内啓光
  • Industrial Property Rights Holder: 国立大学法人東京大学テルモ株式会社
  • Filing Date: 2011-09-09
  • Overseas
• [Patent(Industrial Property Rights)] 血小板産生方法及び血小板産生装置 (2011)
  • Inventor(s): 江藤浩之、中内啓光、福田敏男、高松遼、新井史人
  • Industrial Property Rights Holder: 国立大学法人東京大学国立大学法人名古屋大学
  • Industrial Property Number: 2011-143383
  • Filing Date: 2011-06-28
  • Overseas
• [Patent(Industrial Property Rights)] 血小板産生の場構築の為の巨核球細胞の多核化の促進法 (2011)
  • Inventor(s): 江藤浩之、中内啓光、高山直也、中村壮
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Industrial Property Number: 2011-108253
  • Filing Date: 2011-05-13
  • Overseas
• [Patent(Industrial Property Rights)] ES細胞からの造血前駆細胞を内包する構造物、及び該構造物を用いた血球細胞の調製法 (2011)
  • Inventor(s): 中内啓光、江藤浩之、高山直也、錦井秀和、津久井弘子
  • Industrial Property Rights Holder: 国立大学法人東京大学
  • Filing Date: 2011-11-15
  • Overseas
• [Patent(Industrial Property Rights)] 血小板産生の場構築の為の巨核球細胞の多核化の促進法 (2011)
  • Inventor(s): 江藤浩之
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 2011-108253
  • Filing Date: 2011-05-13
• [Patent(Industrial Property Rights)] 血小板産生方法及び血小板産生装置 (2011)
  • Inventor(s): 江藤浩之
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 2011-143383
  • Filing Date: 2011-06-28
• [Patent(Industrial Property Rights)] 多能性幹細胞からの血液細胞分化に関する培養方法 (2011)
  • Inventor(s): 江藤浩之
  • Industrial Property Rights Holder: 東京大学
  • Filing Date: 2011-09-09
  • Overseas
• [Patent(Industrial Property Rights)] 血小板の機能を維持するための組成物 (2011)
  • Inventor(s): 江藤浩之
  • Industrial Property Rights Holder: 東京大学
  • Filing Date: 2011-09-16
  • Overseas
• [Patent(Industrial Property Rights)] 多能性幹細胞からの巨血球及び/又は血小板の製造方法 (2011)
  • Inventor(s): 江藤浩之
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 2011-219545
  • Filing Date: 2011-10-03
• [Patent(Industrial Property Rights)] 血栓形成を抑制する作用を有する組成物 (2010)
  • Inventor(s): 江藤浩之、西村智、中内啓光、岩倉洋一郎
  • Industrial Property Rights Holder: 陣京大学
  • Industrial Property Number: 2010-180781
  • Filing Date: 2010-08-12
• [Patent(Industrial Property Rights)] 多能性幹細胞からの血液細胞分化に関する培養方法 (2010)
  • Inventor(s): 佐野進弥、江藤浩之、高山直也、中内啓光
  • Industrial Property Rights Holder: 東京大学テルモ株式会社
  • Industrial Property Number: 2010-202752
  • Filing Date: 2010-09-10
• [Patent(Industrial Property Rights)] 分化細胞の新規製造法 (2010)
  • Inventor(s): 江藤浩之、高山直也、中村壮、中内啓光、高山直也、中村壮、中内啓光
  • Industrial Property Rights Holder: 東京大学
  • Filing Date: 2010-09-15
• [Patent(Industrial Property Rights)] ウィルス産生細胞 (2009)
  • Inventor(s): 江藤浩之, 他
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 2009-274595
  • Filing Date: 2009-12-02
• [Patent(Industrial Property Rights)] 分化細胞の新規製造分化細胞の新規製造法 (2009)
  • Inventor(s): 江藤浩之, 他
  • Industrial Property Rights Holder: 東京大学
  • Industrial Property Number: 2009-213645
  • Filing Date: 2009-09-15