Systemic factors responsible for cross-transfer effect of muscle hypertrophy after resistance exercise training

• Project/Area Number: 21300235
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Sports science
• Research Institution: The University of Tokyo
• Principal Investigator: ISHII Naokata 東京大学, 大学院・総合文化研究科, 教授 (20151326)
• Co-Investigator(Kenkyū-buntansha): NAKAZATO Kouichi 日本体育大学, 体育学部, 教授 (00307993) ; OCHI Eusuke 明治学院大学, 教養教育センター, 講師 (90468778) ; NEYA Mitsuo 東京大学, 大学院・総合文化研究科, 助教 (30359640)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2009: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
• Keywords: レジスタンストレーニング / 筋肥大 / 効果転移 / 循環因子 / プロテオーム解析 / 培養筋芽細胞 / シグナル伝達系 / 運動・トレーニング / プオテオーム解析 / 培養筋細胞 / タンパク質合成 / ショットガン解析 / 血流制限

Research Abstract

A low-intensity resistance training for upper arm muscles, if ineffective for muscle hypertrophy, can cause muscle hypertrophy when combined with a resistance training with vascular occlusion for thigh muscles("cross-transfer" of muscle hypertrophy ; Madarame et al., 2008). The present study investigated the role played by systemic factors in this cross-transfer effect. Training experiments with humans showed that conventional, high-intensity resistance training can also cause the similar cross-transfer effect. In addition, proteomic analysis of serum samples taken before and after the training with vascular occlusion showed significant changes in the concentration of several growth factors. When serum was taken from rats subjected to high-intensity resistance training and then added to cultured myoblasts, it caused significant changes in signal transduction substrates related to protein synthesis. These results suggest that some growth factors within serum play important parts in the cross-transfer effect of muscle hypertrophy.

Research Products

• [Journal Article] Roles played by protein metabolism and myogenic progenitor cells in exercise-induced muscle hypertrophy and their relation to resistance training regimens (2012)
  • Author(s): Ishii, N., Ogasawara, R., Kobayashi, K. and Nakazato, K
  • Journal Title: J. Phys. Fitness Sports Med. Volume: 1 Pages: 83-94
  • NAID: 10031158929
  • Peer Reviewed
• [Journal Article] Roles played by protein metabolism and myogenic progenitor cells in exercise-induced muscle hypertrophy and their relation to resistance training regimens (2012)
  • Author(s): Ishii, N., Ogasawara, R., Kobayashi, K., Nakazato, K.
  • Journal Title: Journal of Physical Fitness and Sports Medicine Volume: 1(In press)
  • NAID: 10031158929
  • Peer Reviewed
• [Journal Article] Suppression of UVB-induced HIF-1αup-regulation by hyperoxia does not prevent wrinkle formation associated with increased MMPs activity in mouse skin (2011)
  • Author(s): Kawada, S. and Ishii, N
  • Journal Title: Biomed. Res. Volume: 32 Pages: 363-372
  • Peer Reviewed
• [Journal Article] Blood flow-restricted training does not improve jump performance in untrained young men (2011)
  • Author(s): Madarame H, Ochi E, Tomioka Y, Nakazato K, Ishii N
  • Journal Title: Acta Physiol. Hung. Volume: 98 Pages: 465-471
  • Peer Reviewed
• [Journal Article] Increase in serum growth hormone induced by electrical stimulation of muscle combined with blood flow restriction (2011)
  • Author(s): Inagaki, Y., Madarame, H., Neya, M. and Ishii, N
  • Journal Title: Eur. J. Appl. Physiol. Volume: 111 Pages: 2715-2722
  • Peer Reviewed
• [Journal Article] Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle (2011)
  • Author(s): Ochi. E., Nakazato, K. and Ishii, N
  • Journal Title: J. Str. Condition. Res. Volume: 25 Pages: 2283-2292
  • Peer Reviewed
• [Journal Article] Influence of icing on muscle regeneration after crush injury to skeletal muscles in rats (2011)
  • Author(s): Takagi, R., Fujita, N., Arakawa, T., Kawada, S., Ishii, N. and Miki, A
  • Journal Title: Med, J. Appl. Physiol. Volume: 110 Pages: 382-388
  • Peer Reviewed
• [Journal Article] Acceleration and force reveal different mechanisms of electromechanical delay (2011)
  • Author(s): Sasaki, K., Sasaki, T. and Ishii, N
  • Journal Title: Sci. Sport Exerc. Volume: 43 Pages: 1200-1206
  • Peer Reviewed
• [Journal Article] Muscular hypertrophy and changes in cytokine production after eccentric training rat skeletal muscle (2011)
  • Author(s): Ochi, E., Nakazato, K., Ishii, N.
  • Journal Title: Journal of Strength and Conditioning Research Volume: 25 Issue: 8 Pages: 2283-2292
  • DOI: 10.1519/jsc.0b013e3181f1592e
  • Peer Reviewed
• [Journal Article] Acceleration and force reveal different mechanisms of electromechanical delay (2011)
  • Author(s): Sasaki, K., Sasaki, T., Ishii, N.
  • Journal Title: Medicine and Science in Sports and Exercise Volume: 43 Issue: 7 Pages: 1200-1206
  • DOI: 10.1249/mss.0b013e318209312c
  • Peer Reviewed
• [Journal Article] Ageing related differences in maximum force, unloaded velocity and power of human leg multi-joint movement (2010)
  • Author(s): Yamauchi, J., Mishima, C., Nakayama, S. and Ishii, N
  • Journal Title: Gerontology Volume: 56 Pages: 167-174
  • Peer Reviewed
• [Journal Article] Bilateral Asymmetry in Joint Torque during Squat Exercise Performed by Long Jumpers (2010)
  • Author(s): Kobayashi, Y., Kubo, J., Matsubayashi, T., Matsuo, A., Kobayashi, K. and Ishii, N
  • Journal Title: J. Str. Condition. Res. Volume: 24 Pages: 2826-2830
  • Peer Reviewed
• [Journal Article] Elevation of myostatin and FOXOs in prolonged muscular impairment induced by eccentric contractions in rat medial gastrocnemius muscle (2010)
  • Author(s): Ochi, E., Hirose, T., Hiranuma, K., Min, S., Ishii, N. and Nakazato, K
  • Journal Title: J. Appl. Physiol. Volume: 108 Pages: 306-313
  • Peer Reviewed
• [Journal Article] Endocrine responses to upper-and lower-limb resistance exercises with blood flow restriction (2010)
  • Author(s): Madarame H., Sasaki, K. and Ishii N.
  • Journal Title: Acta Physiologica Hungarica Volume: 97 Pages: 192-200
  • Peer Reviewed
• [Journal Article] Effects of low-intensity resistance exercise with blood flow restriction on coagulation system in healthy subjects (2010)
  • Author(s): Madarame, H., Ishii, N., 他8名
  • Journal Title: Clin. Physiol. Funct. Imag. Volume: 30 Pages: 210-213
  • Peer Reviewed
• [Journal Article] Time course change of IGF-1/Akt/mTOR/p70S6K pathway activation in rat gastrocnemius muscle during repeated bouts of eccentric exercise (2010)
  • Author(s): Ochi, E., Ishii, N. and K. Nakazato
  • Journal Title: J. Sports Sci. Med. Volume: 9 Pages: 170-175
  • Peer Reviewed
• [Journal Article] Increased oxygen tension attenuates acute ultraviolet-B-induced skin angiogenesis and wrinkle formation (2010)
  • Author(s): Kawada, S., Ohtani, M. and Ishii, N
  • Journal Title: Am J. Physiol. Volume: 299
  • Peer Reviewed
• [Journal Article] Unloaded shortening velocity of voluntarily and electrically activated human dorsiflexor muscles in vivo (2010)
  • Author(s): Sasaki, K. and Ishii, N
  • Journal Title: PLoS ONE Volume: 5(9)
  • Peer Reviewed
• [Journal Article] Effects of low-intensity cycle training with restricted leg blood flow on thigh muscle volume and VO2max in young men (2010)
  • Author(s): Abe, T., Fujita, S., Ishii, N
  • Journal Title: J. Sport Sci. Med. Volume: 9 Pages: 452-458
  • Peer Reviewed
• [Journal Article] Elevation of myostatin and FOXOs in prolonged muscular impairment induced by eccentric contractions in rat medial gastrocnemius muscle. (2010)
  • Author(s): Ochi, E., Hirose, T., Hiranuma, K., Min, S., Ishii, N., Nakazato, K.
  • Journal Title: Journal of Applied Physiology Volume: 108 Pages: 306-313
  • Peer Reviewed
• [Journal Article] Endocrine responses to upper-and lower-limb resistance exercises with blood flow restriction. (2010)
  • Author(s): Madarame H., Sasaki, K., Ishii N.
  • Journal Title: Acta Physiologica Hungarica Volume: 97 Pages: 192-200
  • Peer Reviewed
• [Journal Article] Time course change of IGF-1/Akt/mTOR/p70S6K pathway activation in rat gastrocnemius muscle during repeated bouts of eccentric exercise. (2010)
  • Author(s): Ochi, E., Ishii, N., Nakazato.K.
  • Journal Title: Journal of Sports Science and Medicine Volume: 9 Pages: 170-175
  • Peer Reviewed
• [Journal Article] Elevation of myostatin and FOXOs in prolonged muscular impairment induced by eccentric contractions in rat medial gastrocnemius muscle. (2010)
  • Author(s): Ochi, E., Hirose, T., Hiranuma, K., Min, S., Ishii, N., Nakazato, K.
  • Journal Title: J.Appl.Physiol. 108 Pages: 306-313
  • Peer Reviewed
• [Journal Article] Time course change of IGF-1/Akt/mTOR/p7OS6K pathway activation in rat gastrocnemius muscle during repeated bouts of eccentric exercise. (2010)
  • Author(s): Ochi, E., Ishii, N., Nakazato, K.
  • Journal Title: J.Sports Sci.Med. (In press)
  • Peer Reviewed
• [Journal Article] Hormonal and metabolic responses to slow movement resistance exercise with different durations of concentric and eccentric actions (2009)
  • Author(s): Goto, K., Ishii, N., Kizuka, T., Kraemer, R. R., Honda, Y. and Takamatsu, K
  • Journal Title: Eur. J. Appl. Physiol. Volume: 106 Pages: 731-739
  • Peer Reviewed
• [Journal Article] Force-velocity, force-power relationships of bilateral and unilateral leg multi-joint movements in young and elderly women (2009)
  • Author(s): Yamauchi, J., Mishima, C., Nakayama, S. and Ishii, N
  • Journal Title: J. Biomech. Volume: 41 Pages: 2151-2157
  • Peer Reviewed
• [Journal Article] Laser flash photolysis of diazo-2, a caged calcium chelator : The relationship between the extent and rate of smooth muscle relaxation (2009)
  • Author(s): Pelc, R., Ishii, N. and Ashley, C. C
  • Journal Title: J. Laser Applications Volume: 21 Pages: 32-38
  • Peer Reviewed
• [Journal Article] Peripheral venous occlusion causing cardiac hypertrophy and changes in biological parameters in rats (2009)
  • Author(s): Kawada, S. and Ishii, N
  • Journal Title: Eur. J. Appl. Physiol. Volume: 105 Pages: 909-917
  • Peer Reviewed
• [Journal Article] Significant roles of microtubules in mature striated muscle deduced from the correlation between tubulin and its molecular chaperone alpha B-crystallin in rat muscles (2009)
  • Author(s): Jee, H., Sakurai, T., Kawada, S., Ishii, N. and Atomi, Y
  • Journal Title: J. Physiol. Sci. Volume: 59 Pages: 149-155
  • Peer Reviewed
• [Journal Article] Low-intensity resistance training with slow movement and tonic force generation(LST) increases basal limb blood flow (2009)
  • Author(s): Tanimoto. M., Ishii, N., 他6名
  • Journal Title: Clinical Physiol. Funct Imag Volume: 29 Pages: 128-135
  • Peer Reviewed
• [Journal Article] Changes in muscle activation and force generation patterns during cycling movements due to low-intensity squat training with slow movement and tonic force generation(LST) (2009)
  • Author(s): Tanimoto. M., Miyachi, M. and Ishii, N
  • Journal Title: J. Str. Conditon. Res. Volume: 23 Pages: 2367-2376
  • Peer Reviewed
• [Journal Article] Peripheral venous occlusion causing cardiac hypertrophy and changes in biological parameters in rats. (2009)
  • Author(s): Kawada, S., Ishii, N.
  • Journal Title: Eur.J.Appl.Physiol. 105 Pages: 909-917
  • Peer Reviewed
• [Presentation] リハビリテーションのための低負荷強度筋力トレーニング (2011)
  • Author(s): 石井直方
  • Organizer: 日本柔道整復接骨医学会大会
  • Place of Presentation: 千葉幕張メッセ
  • Year and Date: 2011-10-23
• [Presentation] リハビリテーションのための低負荷強度筋力トレーニング (2011)
  • Author(s): 石井直方
  • Organizer: 日本柔道整復接骨医学会大会
  • Place of Presentation: 千葉幕張メッセ(招待講演)
  • Year and Date: 2011-10-23
• [Presentation] 筋力増強の・カニズムとその応用 (2010)
  • Author(s): 石井直方
  • Organizer: 第114回中部日本整形外科災害学会
  • Place of Presentation: 名古屋国際会識場
  • Year and Date: 2010-04-09
• [Book] ボディメンテナンスメソッド (2010)
  • Author(s): 石井直方
  • Total Pages: 159
  • Publisher: 成美堂出版(東京)
• [Remarks]
  • URL: http://www.c.u-tokyo.ac.jp/info/research/faculty/list/mds/mds-ls/f002506.html