Screening of foods with anti-diabetic-complications function and elucidation of their functionality and mechanism of the function

• Project/Area Number: 21300278
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Eating habits, studies on eating habits
• Research Institution: Kochi University
• Principal Investigator: YAGI Toshiharu 高知大学, 教育研究部・自然科学系, 教授 (90110759)
• Co-Investigator(Kenkyū-buntansha): TODA Katsumi 高知大学, 教育研究部・医療学系, 准教授 (40197893)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 健康と食生活 / ピリドキサミン / 糖尿病合併症 / 糖新生 / 食品 / ビタミンB6 / 抗糖尿病合併症

Research Abstract

Pyridoxamine(PM), one of vitamin B_6, has anti-diabetic-complications function. Foods containing a lot of PM, thus, may have this function. At first, a novel determination method of PM in foods has been developed. PM contents in several foods were determined. Then, PM was supplied to mice, and expression levels of genes encoding gluconeogenesis-related proteins. PM increased gluconeogenesis in the mice.

Research Products

• [Journal Article] Abnormality in expression levels of gluconeogenesis-related genes by high-dose supplementation with pyridoxamine in mice (2012)
  • Author(s): H. T. V. Do, K. Toda, T. Saibara, T. Yagi
  • Journal Title: International Journal for Vitamin and Nutrition Research Volume: (in press)
  • Peer Reviewed
• [Journal Article] All-enzymatic HPLC method for determination of individual and total contents of vitamin B_6 in foods (2012)
  • Author(s): H. T. V. Do, Y. Ide, A. N. Mugo, T. Yagi
  • Journal Title: Food and Nutrition Research Volume: 55 Pages: 5409-5416
  • Peer Reviewed
• [Journal Article] Individual vitamin B_6 contents in selected Japanese sushi toppings (2012)
  • Author(s): H. T. V. Do, T. Yagi
  • Journal Title: International Journal of Food Sciences and Nutrition Volume: 63(2) Pages: 246-249
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of pyridoxine 4-oxidase (2012)
  • Author(s): Mugo, A. N., Kobayashi, J., Mikami, B., Ohnishi, K., Yagi T.
  • Journal Title: the first enzyme in pyridoxine degradation pathway I. Acta Crystallographica. Section F Volume: 68(1) Pages: 66-68
  • Peer Reviewed
• [Journal Article] ビタミンB_6測定法の現状と課題(総説) (2011)
  • Author(s): 八木年晴
  • Journal Title: ビタミン Volume: 85(3) Pages: 116-126
  • Peer Reviewed
• [Journal Article] The crystal structure of SDR-type pyridoxal 4-dehydrogenase of Mesorhizobium loti (2011)
  • Author(s): Chu, H. N., Kobayashi, J., Mikami, B., Yagi, T.
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 75(2) Pages: 388-390
  • NAID: 10027898248
  • Peer Reviewed
• [Journal Article] All-enzymatic HPLC method for determination of individual and total contents of vitamin B6 in foods (2011)
  • Author(s): Do, H.T.V., Y.Ide, Mugo, A.N., Yagi, T.
  • Journal Title: Food & Nutrition Research Volume: 55 Pages: 5409-5416
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of pyridoxine 4-oxidase, the first enzyme in pyridoxine degradation pathway I (2011)
  • Author(s): Mugo A.N., Kobayashi J., Mikami B., Ohnishi K., Yagi T.
  • Journal Title: Acta Crystallographica Section F Volume: 68 Issue: 1 Pages: 66-68
  • DOI: 10.1107/s1744309111048688
  • Peer Reviewed
• [Journal Article] The Crystal Structure of SDR-Type Pyridoxal 4-Dehydrogenase of Mesorhizobium loti (2011)
  • Author(s): Chu, H.N., Kobayashi, J., Mikami, B., Yagi, T.
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 2 Pages: 388-390
  • DOI: 10.1271/bbb.100748
  • NAID: 10027898248
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] ビタミンB6測定法の現状と課題 (2011)
  • Author(s): 八木年晴
  • Journal Title: ビタミン Volume: 85 Pages: 116-126
  • Peer Reviewed
• [Journal Article] Development of simultaneous enzymatic assay method for all six individual vitamin B_6 forms and pyridoxine-β-glucoside (2010)
  • Author(s): Yagi, T., Murayama, R., Do, H. T. V., Ide, Y., Mugo, A. M., Yoshikane, Y.
  • Journal Title: J. Nutr. Sci. Vitaminol Volume: 56(3) Pages: 157-163
  • NAID: 10030703710
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti (2010)
  • Author(s): Chu, H. N., Kobayashi, J., Yoshikane, Y., Mikami, B., Yagi, T.
  • Journal Title: Acta Crystallographica Section F Volume: 66(6) Pages: 718-720
  • Peer Reviewed
• [Journal Article] Topology of 4-Pyridoxic acid dehydrogenase in transformed Escherichia coli cells. (2010)
  • Author(s): Ge F., Yagi, T
  • Journal Title: J.Biochem. Volume: 147 Pages: 291-295
  • NAID: 10027874097
  • Peer Reviewed
• [Journal Article] Engineering Mesorhizobium loti pyridoxamine-pyruvate aminotransferase for production of pyridoxamine with 1-glutamate as an amino donor (2010)
  • Author(s): Yoshikane, Y., Tamura, A., Yokochi, N., Ellouze, K., Yamamura, E., Mizunaga, H., Fujimoto, N., (…), Yagi, T.
  • Journal Title: J.Mol.Cat.Enz. Volume: 67 Pages: 104-110
  • Peer Reviewed
• [Journal Article] Development of simultaneous enzymatic assay method for all six individual vitamin B6 forms and pyridoxine-β-glucoside. (2010)
  • Author(s): Yagi, T., Murayama, R., Do, H.T.V., Ide, Y., Mugo, A.M., Yoshikane, Y.
  • Journal Title: J.Nutr.Sci.Vitaminol. Volume: 56 Pages: 157-163
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of SDR-type pyridoxal dehydrogenase from Mesorhizobium loti. (2010)
  • Author(s): Chu, H.N., Kobayashi, J., Yoshikane, Y., Mikami, B., Yagi, T.
  • Journal Title: Aca.Cryst.Sec.F Volume: 66 Pages: 718-720
  • Peer Reviewed
• [Journal Article] Esmond E.Snell-The pathfinder of B vitamins and cofactors. (2010)
  • Author(s): Hayashi, H., Tanase, S., Yagi, T.
  • Journal Title: J.Biochem. Volume: 147 Pages: 451-457
  • Peer Reviewed
• [Journal Article] Development of simultaneous enzymatic assay method for all of the six individual vitamin B6 and pyridoxine-β-glucoside (2010)
  • Author(s): Yagi, T, Ryutaro Murayama, Huong Do Thi Viet, Youhei Ide, Andrew Njagi Mugo, Yu Yoshikane
  • Journal Title: J.Nutr.Sci.Vitaminol. 56(In press)
  • Peer Reviewed
• [Journal Article] Topology of 4-Pyridoxic acid dehydrogenase in transformed Escherichia coli cells (2010)
  • Author(s): Ge, F., Yagi T.
  • Journal Title: J.Biochem. 147 Pages: 291-295
  • NAID: 10027874097
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of 4-Pyridoxolactonase from Mesorhizobium loti (2009)
  • Author(s): Matsuda, S., Yokochi, N., Yoshikane, Y., Kobayashi, J., Huy, C. N., Baba, S., Kuramitsu, S., (...), Yagi, T.
  • Journal Title: Acta Crystal. Sect Volume: F 65(9) Pages: 886-889
  • NAID: 120001671533
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway i of pyridoxine (2009)
  • Author(s): Kobayashi, J., Yoshida, H., Chu, H. N., Yoshikane, Y., Kamitori, S., Yagi, T.
  • Journal Title: Acta Crystal. Se Volume: F 65(8) Pages: 829-831
  • NAID: 120001671534
  • Peer Reviewed
• [Journal Article] Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase (2009)
  • Author(s): Yokochi, N., Yoshikane, Y., Matsumoto, S., Fujisawa, M., Ohnishi, K., Yagi, T.
  • Journal Title: J. Biochem Volume: 145(4) Pages: 493-503
  • NAID: 10026302164
  • Peer Reviewed
• [Journal Article] Gene identification and characterization of 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid 5-dehydrogenase, an NAD+-dependent dismutase (2009)
  • Author(s): Yokochi, N., Yoshikane, Y., Matsumoto, S., Fujisawa, M., Ohnishi, K., Yagi, T.
  • Journal Title: J.Biochem. 145 Pages: 493-503
  • NAID: 10026302164
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of 4-Pyridoxolactonase from Mesorhizobium loti (2009)
  • Author(s): Matsuda, S., Yokochi, N., Yoshikane, Y., Kobayashi, J., Huy, C.N., Baba, S., Kuramitsu, S., Yagi, T.
  • Journal Title: Acta Cryst.F 65 Pages: 886-889
  • NAID: 120001671533
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray analysis of AAMS amidohydrolase, the final enzyme in degradation pathway i of pyridoxine (2009)
  • Author(s): Kobayashi, J., Yoshida, H., Chu, H.N., Yoshikane, Y., Kamitori, S., Yagi, T.
  • Journal Title: Acta Cryst.F. 65 Pages: 829-831
  • NAID: 120001671534
  • Peer Reviewed
• [Presentation] 牛乳と人乳中の各種ビタミンB_6化合物含有量について (2011)
  • Author(s): 八木年晴、岸貴之、井手陽平、Do Thi Viet Huong
  • Organizer: 日本ビタミン学会第63回大会
  • Place of Presentation: 安田女子大学
  • Year and Date: 2011-06-05
• [Presentation] ヒト尿中ビタミンB6分別定量法 (2010)
  • Author(s): 村山隆太郎, Do Thi Viet Huong, 井手陽平, 吉金優, 八木年晴
  • Organizer: 日本農芸化学会中四国支部例会
  • Place of Presentation: 愛媛大学(松山)
  • Year and Date: 2010-01-23
• [Presentation] 膜酵素4-ピリドキシン酸デヒドロゲナーゼのCoQ結合部位 (2010)
  • Author(s): 鈴木太郎, 葛飛, 小林淳, 吉金優, 八木年晴
  • Organizer: 日本農芸化学会中四国支部例会
  • Place of Presentation: 愛媛大学(松山)
  • Year and Date: 2010-01-23
• [Presentation] Contents of six natural vitamin B_6 and pyridoxine-β-glucoside in some foods determined by all-enzymatic HPLC method (2010)
  • Author(s): Huong Do Thi Viet, Youhei Ide, Ryutaro Murayama, Andrew Njagi Mugo, Toshiharu Yagi.
  • Organizer: 日本ビタミン学会第62回大会
  • Place of Presentation: 岩手県民情報交流センター
• [Presentation] ビタミンB_6分解酵素4-pyrodoxolactonaseのX線結晶構造解析 (2010)
  • Author(s): 小林淳、吉金優、馬場清喜、三上文三、八木年晴
  • Organizer: 日本生化学会第83回大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] Crystal structure of SDR-type pyridoxal 4-dehydrogenase from Mesorhizobium loti (2010)
  • Author(s): Huy Nhat Chu, Jun Kobayashi, Yu Yoshikane, Toshiharu Yagi, Bunzo Mikami
  • Organizer: 日本生化学会第83回大会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] All-enzymatic HPLC method for determination of individual and total contents of vitamin B_6 in foods (2010)
  • Author(s): Toshiharu Yagi
  • Organizer: The first international vitamin conference(Copenhagen)
  • Place of Presentation: IDA Conference Center, Copenhagen, Denmark
• [Presentation] All-enzymatic HPLC method for determination of individual and total contents of vitamin B6 in foods. (2010)
  • Author(s): Toshiharu Yagi
  • Organizer: The first international vitamin conference(Copenhagen)
  • Place of Presentation: コペンハーゲン
• [Presentation] Contents of six natural vitamin B6 and pyridoxine-β-glucoside in some foods determined by all-enzymatic HPLC method.. (2010)
  • Author(s): Huong Do Thi Viet, Youhei Ide, Ryutaro Murayama, Andrew Njagi Mugo, Toshiharu Yagi.
  • Organizer: 日本ビタミン学会第62回大会
  • Place of Presentation: 岩手県民情報交流センター
• [Presentation] ビタミンB6分解酵素4-pyrodoxolactonaseのX線結晶構造解析 (2010)
  • Author(s): 小林淳、吉金優、馬場清喜、三上文三、八木年晴
  • Organizer: 日本生化学会第83回大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] Crystal Structure of SDR-type pyridoxal 4-dehydrogenase from Mesorhizobium loti (2010)
  • Author(s): Huy Nhat Chu, Jun Kobayashi, Yu Yoshikane, Toshiharu Yagi, Bunzo Mikami
  • Organizer: 日本生化学会第83回大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
• [Presentation] 高圧塩酸処理を行わない酵素-HPLC法による食品中ビタミンB6分別定量 (2009)
  • Author(s): 井手陽平, 村山隆太郎, Do Thi Viet Huong, 吉金優, 八木年晴
  • Organizer: 日本農芸化学会関西・中四国・西日本支部合同大会
  • Place of Presentation: 琉球大学(沖縄)
  • Year and Date: 2009-10-31
• [Presentation] Topology Structure and Biochemical Function of 4-Pyridoxic Acid Dehydrogenase in Bacteria (2009)
  • Author(s): Ge Fei, Yu Yoshikane, Kouhei Ohnishi, Toshiharu Yagi
  • Organizer: 21stIUBMB & 12^<th> FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: 国際会議場(上海)
  • Year and Date: 2009-08-04
• [Presentation] PLP、PMP、PNP、PNGの酵素HPLC法による定量条件の改良II (2009)
  • Author(s): 井手陽平、小林淳、村山隆太郎、吉金優、八木年晴
  • Organizer: ビタミン学会大会
  • Place of Presentation: 亀岡
  • Year and Date: 2009-05-31
• [Presentation] PLP、PMP、PNP、PNGの酵素HPLC法による定量条件の改良II (2009)
  • Author(s): 井手陽平, 小林淳, 村山隆太郎, 吉金優, 八木年晴
  • Organizer: 日本ビタミン学会大会
  • Place of Presentation: 京都学園大学(亀岡)
  • Year and Date: 2009-05-31
• [Presentation] ピリドキシン分解酵素4-ピリドキシン酸デヒドロゲナーゼの膜トポロジー (2009)
  • Author(s): 葛飛、吉金優、八木年晴
  • Organizer: 農芸化学会大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Book] ビタミン総合辞典 (2010)
  • Author(s): 日本ビタミン学会
  • Total Pages: 624
  • Publisher: 朝倉書店
• [Remarks]
  • URL: http://www.iic.kochi-u.ac.jp/ipc/research/home.htm
• [Patent(Industrial Property Rights)] ビタミンB6の分別定量法およびビタミンB6の分別定量用キット (2009)
  • Inventor(s): 八木年晴
  • Industrial Property Rights Holder: 高知大学
  • Industrial Property Number: 2009-233631
  • Filing Date: 2009-09-07