Risk assessment of environmental oxidative chemicals using catalase mutant primary cultured hepatocytes

• Project/Area Number: 21310022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental impact assessment/Environmental policy
• Research Institution: Okayama University
• Principal Investigator: WANG Da-hong 岡山大学, 大学院・医歯薬学総合研究科, 助教 (90294404)
• Co-Investigator(Kenkyū-buntansha): FUJITA Hirofumi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (20423288) ; OGINO Keiki 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70204104) ; TSUTSUI Ken 岡山大学, 自然生命科学研究支援センター, 教授 (70108158) ; SANO Kuniaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00294405) ; NAKAMURA Kazuyuki 山口大学, 医学(系)研究科, 教授 (90107748) ; MIYAZAKI Masahiro 岡山学院大学, 人間生活学部, 教授 (90116509)
• Co-Investigator(Renkei-kenkyūsha): TSUTSUI Ken 岡山大学, 自然生命科学研究支援センター, 教授 (70108158) ; SANO Kuniaki 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00294405) ; NAKAMURA Kazuyuki 山口大学, 医学(系)研究科, 教授 (90107748) ; MIYAZAKI Masahiro 岡山学院大学, 人間生活学部, 教授 (90116509) ; MASUOKA Noriyoshi 岡山理科大学, 理学部, 教授 (20116502)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
• Keywords: 初代培養肝細胞 / カタラーゼ / 過酸化水素 / ヒドロキノン / アポトーシス / ローソン / レスベラトロール / 化学物質の生体影響評価 / 生体影響評価 / カタラーゼ遺伝子変異マウス / プロテオーム / 美白クリーム / 酸化ストレス / 環境影響評価

Research Abstract

We exposured mouse primary hepatocytes to chemicals that are commonly used in our daily lives like hydroquinone, lawsone. We found that exposure the cells to these chemicals decreased cellular viability in both catalase mutant mice (Cs^b) and the wild-type mice (Cs^a) dose-dependently, in particular, the survival of hepatocytes was lower in Csbwhen compared to that of Cs^a. We also observed that exposure the cells to hydroquinone, a main component of skin-lightening cream, enhanced the cellular expression of cytochrome P450 2E1 mRNA and protein dose-dependently as well as induced apoptosis of the cells.

Research Products

• [Journal Article] Effect of vitamin E on alloxan-induced mouse diabetes (2013)
  • Author(s): Kamimura W, Doi W, Takemoto K, Ishihara K, Wang DH, Sugiyama H, OdaSI, Masuoka N
  • Journal Title: Clinical Biochemistry
  • Peer Reviewed
• [Journal Article] Effect of vitamin E on alloxan-induced mouse diabetes. (2013)
  • Author(s): Kamimura W, Doi W, Takemoto K, Ishihara K, (Wang DH), Sugiyama H, Oda SI, Masuoka N
  • Journal Title: Clinical Biochemistry Volume: 3 Issue: 9 Pages: 1-6
  • DOI: 10.1016/j.clinbiochem.2013.02.016
  • Peer Reviewed
• [Journal Article] Resveratrol Inhibited Hydroquinone- induced Cytotoxicity in Mouse Primary Hepatocytes (2012)
  • Author(s): Wang DH, Ootsuki Y, Fujita H, Miyazaki M, Yie Q, Tsutsui K, Sano K, Masuoka N, Ogino K
  • Journal Title: Int. J. Environ Res & Public Health Volume: 9 Pages: 3354-3364
  • Peer Reviewed
• [Journal Article] Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis (2012)
  • Author(s): Takiue K, Sugiyama H, Inoue T, Morinaga H, Kikumoto Y, Kitagawa M, Kitamura S, Maeshima Y, Wang DH, Masuoka N, Ogino K, Makino H
  • Journal Title: BMC Nephrol. Volume: 13 Pages: 14-14
  • Peer Reviewed
• [Journal Article] Resveratrol Inhibited Hydroquinone-induced Cytotoxicity in Mouse Primary Hepatocytes. (2012)
  • Author(s): (Wang DH), Otsuki Y, Fujita H, Miyazaki M, Yie Q, Tsutsui K, Sano K, Masuoka N, Ogino K.
  • Journal Title: Int J Environ Res & Public Health Volume: 9 Issue: 9 Pages: 3354-3364
  • DOI: 10.3390/ijerph9093354
  • Peer Reviewed
• [Journal Article] Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis. (2012)
  • Author(s): Takiue K, Sugiyama H, Inoue T, Morinaga H, Kikumoto Y, Kitagawa M, Kitamura S, Maeshima Y, (Wang DH), Masuoka N, Ogino K, Makino H.
  • Journal Title: BMC Nephrology Volume: 13 Pages: 14-23
  • Peer Reviewed
• [Journal Article] Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis (2012)
  • Author(s): Keiichi Takiue, Hitoshi Sugiyama, Tatsuyuki Inoue, Hiroshi Morinaga, Yoko Kikumoto, Masashi Kitagawa, Shinji Kitamura, Yohei Maeshima, Da-Hong Wang, Noriyoshi Masuoka, Keiki Ogino, Hirofumi Makino
  • Journal Title: BMC Nephrology Volume: 13 Pages: 14-23
  • Peer Reviewed
• [Journal Article] A new risk assessment method for evaluation of oxidative chemicals using catalase mutant mouse primary hepatocytes (2011)
  • Author(s): Wang DH, Ishikawa Y, Miyazaki M, Fujita H, Tsutsui K, Sano K, Masuoka N, Ogino K
  • Journal Title: Health Volume: 3 Pages: 288-291
  • Peer Reviewed
• [Journal Article] High serum arginase I levels in asthma : its correlation with high-sensitivity C-reactive protein (2011)
  • Author(s): Ogino K, Obase Y, Takahashi N, Shimizu H, Takigawa T, Wang DH, Ouchi K, Oka M
  • Journal Title: J Asthma Volume: 48 Pages: 1-7
  • Peer Reviewed
• [Journal Article] The Role of Catalase in Pulmonary Fibrosis (2010)
  • Author(s): Odajima N, Betsuyaku T, Nagai K, Moriyama C, Wang DH, Takigawa T, Ogino K, Nishimura M
  • Journal Title: Respiratory Res Volume: 11 Pages: 183-183
  • Peer Reviewed
• [Journal Article] (2010)
  • Author(s): Kikumoto Y, Sugiyama H, Inoue T, Morinaga H, Takiue K, Kitagawa M, Fukuoka N, Saeki M, Maeshima Y, Wang DH, Ogino K, Masuoka N, Makino H
  • Journal Title: Biochim. Biophys. Acta Volume: 1802 Pages: 240-246
  • Peer Reviewed
• [Journal Article] Evaluation of Pyrogallol-induced Cytotoxicity in Catalase-mutant Escherichia coli and Mutagenicity in Salmonella typhimurium. Bulletin of Environmental Contamination and Toxicology (2010)
  • Author(s): Takemura Y, Wang DH, Sauriasari R, Horita M,Tsutsui K, Sano K, Masuoka N, Takigawa T, Takaki J, Ogino K
  • Journal Title: Bull. Environ. Contam. Toxicol Volume: 84 Pages: 347-350
  • Peer Reviewed
• [Journal Article] The Role of Catalase in Pulmonary Fibrosis. (2010)
  • Author(s): Odajima N, Betsuyaku T, Nagai K, Moriyama C, Wang DH, Takigawa T, Ogino K, Nishimura M.
  • Journal Title: Respiratory Research Volume: 11(183) Pages: 1-13
  • Peer Reviewed
• [Journal Article] Sensitization to alloxan-induced diabetes and pancreatic cell apoptosis in acatalasemic mice. Biochim Biophys Acta. 2010 ; 1802(2) : 240-6. (2010)
  • Author(s): Kikumoto Y, Sugiyama H, Inoue T, Morinaga H, Takiue K, Kitagawa M, Fukuoka N, Saeki M, Maeshima Y, Wang DH, Ogino K, Masuoka N, Makino H.
  • Journal Title: Biochim Biophys Acta. Volume: 1802(2) Pages: 240-246
  • Peer Reviewed
• [Journal Article] Direct inhibition of arginase attenuated airway allergic reactions and inflammation in a Dermatophagoides farinae induced NC/Nga mouse model. (2010)
  • Author(s): Takahashi N, Ogino K, Takemoto K, Hamanishi S, Wang DH, Takigawa T, Shibamori M, Ishiyama H, Fujikura Y.
  • Journal Title: Am J Physiol Lung Cell Mol Physiol. Volume: 299(1)
  • Peer Reviewed
• [Journal Article] Evaluation of Pyrogallol-induced Cytotoxicity in Catalase-mutant Escherichia coli and Mutagenicity in Salmonella typhimurium. (2010)
  • Author(s): Takemura Y, Wang DH, Sauriasari R, Horita M, Tsutsui K, Sano K, Masuoka N, Takigawa T, Takaki J, Ogino K.
  • Journal Title: Bulletin of Environmental Contamination and Toxicology 84 Pages: 347-350
  • Peer Reviewed
• [Journal Article] Sensitization to alloxan-induced diabetes and pancreatic cell apoptosis in acatalasemic mice. (2010)
  • Author(s): Kikumoto Y, Sugiyama H, Inoue T, Morinaga H, Takiue K, Kitagawa M, Fukuoka N, Saeki M, Maeshima Y, Wang DH, Ogino K, Masuoka N, Makino H.
  • Journal Title: Biochim Biophys Acta 1802 Pages: 240-246
  • Peer Reviewed
• [Journal Article] Low catalase activity in blood is associated with the diabetes caused by alloxan (2009)
  • Author(s): Takemoto K, Tanaka M, Iwata H, Nishihara R, Ishihara K, Wang DH, Ogino K, Taniuchi K, Masuoka N
  • Journal Title: Clin. Chim. Acta Volume: 407 Pages: 43-46
  • Peer Reviewed
• [Presentation] Risk assessment of environmental oxidative chemicals using catalase mutant primary cultured hepatocytes (2012)
  • Author(s): Wang DH, Miyazaki M, Fujita H, Tsutsui K, Sano K, Masuoka N, Ogino K
  • Organizer: The American Public Health Association's 140th Annual Meeting and Exposition, The Moscone Convention Center West
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 2012-10-29
• [Presentation] 初代培養肝細胞におけるヒドロキノンの影響とレスベラトロールの抑制効果 (2012)
  • Author(s): 叶欽霞、汪達紘、大月芳恵、石川靖夫、藤田洋史、宮崎正博、筒井研、佐野訓明、 益岡典芳、荻野景規
  • Organizer: 第82回日本衛生学会学術総会
  • Place of Presentation: 京都大学吉田キャンパス
  • Year and Date: 2012-03-25
• [Presentation] ダニ蛋白誘発喘息におけるL-アルギニンの効果 (2012)
  • Author(s): 張燃、村上育郎、高橋秀和、汪達紘
  • Organizer: 第82回日本衛生学会学術総会
  • Year and Date: 2012-03-25
• [Presentation] Risk assessment of environmental oxidative chemicals using catalase mutant primary cultured hepatocytes. (2012)
  • Author(s): (Dahong Wang), Masahiro Miyazaki, Hirofumi Fujita, Ken Tsutsui, Kuniaki Sano, Noriyoshi Masuoka, Keiki Ogino
  • Organizer: The American Public Health Association's 140th Annual Meeting and Exposition.
  • Place of Presentation: The Moscone Convention Center West, San Francisco, CA, USA
• [Presentation] 新しい喘息予防法に関する研究 (2011)
  • Author(s): 張燃、村上育郎、高橋秀和、汪達紘、 荻野景規
  • Organizer: 第9回日本予防医学会学術総会
  • Place of Presentation: 首都大学東京 荒川キャンパス
  • Year and Date: 2011-11-20
• [Presentation] 新しい喘息予防法に関する研究 (2011)
  • Author(s): 張燃、村上育郎、高橋秀和、汪達紘、荻野景規
  • Organizer: 第9回日本予防医学会学術総会
  • Place of Presentation: 首都大学東京荒川キャンパス
  • Year and Date: 2011-11-20
• [Presentation] 健康成人及び喘息患者における血清3-nitrotyrosineの疫学的評価 (2011)
  • Author(s): 荻野景規、高橋紀子、ヘリサテワン、赤沢裕之、高橋秀和、汪達紘
  • Organizer: 第17回MPO研究会
  • Place of Presentation: アークホテル熊本
  • Year and Date: 2011-10-29
• [Presentation] 低カタラーゼ活性マウスの初代培養肝細胞を用いた環境化学物質評価法の開発(第2報) (2011)
  • Author(s): 大月芳恵、汪達紘、石川靖夫、藤田洋史、宮崎正博、筒井研、佐野訓明、瀧川智子、荻野景規
  • Organizer: 第81回日本衛生学会学術総会
  • Place of Presentation: 昭和大学旗の台キャンパス
  • Year and Date: 2011-08-26
• [Presentation] 低カタラーゼ活性マウスの初代培養肝細胞を用いた環境化学物質評価法の開発 (第2報) (2011)
  • Author(s): 大月芳恵、汪達紘、石川靖夫、藤田洋史、宮崎正博、筒井研、佐野訓明、瀧川智子、荻野景規
  • Organizer: 第81回日本衛生学会学術総会
  • Place of Presentation: 昭和大学旗の台キャンパス
  • Year and Date: 2011-03-26
• [Presentation] カタラーゼ活性の異なるマウスの初代培養肝細胞に及ぼすヒドロキノンの影響 (2010)
  • Author(s): 大月芳恵、汪達紘、石川靖夫、藤田洋史、宮崎正博、筒井研、佐野訓明、瀧川智子、荻野景規
  • Organizer: 第8回日本予防医学会学術総会
  • Place of Presentation: 石川県立音楽堂 交流ホール
  • Year and Date: 2010-12-12
• [Presentation] カタラーゼ括性の異なるマウスの初代培養肝細胞に及ぼすヒドロキノンの影響 (2010)
  • Author(s): 大月芳恵、汪達紘、石川靖夫、藤田洋史、宮崎正博、筒井研、佐野訓明、瀧川智子、荻野景規
  • Organizer: 第8回日本予防医学会学術総会
  • Place of Presentation: 石川県立音楽堂 交流ホール
  • Year and Date: 2010-12-12
• [Presentation] Low catalase activity is higher incidence of diabetes. (2010)
  • Author(s): Kazunori Takemoto, Tanaka M, Iwata H, Nishihara R, Ishihara K, Wang DH,Ogino K, Taniuchi K, Masuoka N
  • Organizer: International Conference on Biologically Active Substances
  • Place of Presentation: 岡山県立大学(総社)
  • Year and Date: 2010-08-11
• [Presentation] Low catalase activity is higher incidence of diabetes. (2010)
  • Author(s): Kazunori Takemoto, Tanaka M, Iwata H, Nishihara R, Ishihara K, Wang DH, Ogino K, Taniuchi K, Masuoka N.
  • Organizer: International Conference on Biologically Active Substances.
  • Place of Presentation: 岡山県立大学(総社)
  • Year and Date: 2010-08-11
• [Presentation] 低カタラーゼ活性マウスの初代培養肝細胞を用いた環境化学物質評価法の開発(第1報) (2010)
  • Author(s): 汪達紘、石川靖夫、宮崎正博、藤田洋史、筒井研、佐野訓明、益岡典芳、瀧川智子、高木二郎、荻野景規
  • Organizer: 第80回日本衛生学会学術総会
  • Place of Presentation: 仙台市国際センター