Analysis of flower coloration and control mediated by multi-enzyme complexes

• Project/Area Number: 21310135
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Tohoku University
• Principal Investigator: NAKAYAMA Toru 東北大学, 大学院・工学研究科, 教授 (80268523)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2009: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
• Keywords: メタボロン / フラボノイド / 酵素間相互作用 / キンギョソウ / フラボン / アント シアニン / オーロン / 代謝 / 酵素 / 相互作用 / アントシアニン / 花色発現 / 二次代謝 / 代謝複合体 / タンパク質間相互作用 / BAHDファミリー / シトクロムP450 / カルコン合成酵素 / カルコンイソメラーゼ

Research Abstract

I have clarified that, in snapdragon petal cell, enzymes that are related to biosynthesis of flavones and anthocyanins form multi-enzyme complexes, which are spatially and functionally separated from enzymes related to aurone biosynthesis.

Research Products

• [Journal Article] Transcription analyses of GmICHG, a gene coding for a β-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr (2013)
  • Author(s): Yoo, D.C., Hara, T., Fujita, N., Waki, T., Noguchi, A., Takahashi, S., Nakayama, T.
  • Journal Title: Plant Sci Volume: 208 Pages: 10-19
  • URL: http://dx.doi.org/10.1016/j.plantsci.2013.03.006
  • Peer Reviewed
• [Journal Article] Purification, gene cloning, and biochemical characterization of a β-glucosidase capable of hydrolyzing sesaminol triglucoside from Paenibacillus sp. KB0549 (2013)
  • Author(s): Nair, A., Kuwahara, A. Nagase, A. Yamaguchi, H. Yamazaki, T., Hosoya, M., Omura, A., Kiyomoto, K., Yamaguchi, M., Shimoyama, T., Takahashi, S., and Nakayama, T.
  • Journal Title: PLOS ONE Volume: 8 Issue: 4 Pages: e60538-e60538
  • DOI: 10.1371/journal.pone.0060538
  • Peer Reviewed
• [Journal Article] Crystallization and preliminary X-ray crystallographic analysisof UDP-glucuronic acid :flavonol-3-O-glucuronosyltransferase (2013)
  • Author(s): Mizohata, E., Okuda, T., Hatanaka, S., Nakayama, T., Horikawa, M., Nakayama, T., Ono, E., and Inoue, T.
  • Journal Title: (VvGT5) from the grapevine Vitis vinifera. Acta Cryst Volume: F69 Issue: 1 Pages: 65-68
  • DOI: 10.1107/s1744309112045095
  • Peer Reviewed
• [Journal Article] Synthesis of 3,5,3′,4′-Tetrahydroxy-trans-stilbene-4′-O-b-D-glucopyranoside by Glucosyltransferases from Phytolacca americana (2013)
  • Author(s): Tomoya Iwakiri
  • Journal Title: Natural Product Communications Volume: 8 Pages: 119-120
  • Peer Reviewed
• [Journal Article] Transcription analyses of GmICHG, a gene coding for a b-glucosidase that catalyzes the specific hydrolysis of isoflavone conjugates in Glycine max (L.) Merr (2013)
  • Author(s): DongChan Yoo
  • Journal Title: Plant Science Volume: 208 Pages: 10-19
  • DOI: 10.1016/j.plantsci.2013.03.006
  • Peer Reviewed
• [Journal Article] メタボロン…植物二次代謝工学におけるインパクト (2012)
  • Author(s): 中山亨*・兪東燦・高橋征司
  • Journal Title: 生物工学 Volume: 90 Pages: 576-581
  • NAID: 110009517335
• [Journal Article] Synthesis of3,5,3′,4′-tetrahydroxy- trans-stilbene-4′-O-β-D-glucopyranoside by glucosyltransferases from Phytolacca Americana Nat (2012)
  • Author(s): Iwakiri, T., Imai, H., Hamada H., Nakayama, T. and Ozaki, S.
  • Journal Title: Prod. Commun Volume: 8 Pages: 119-120
  • Peer Reviewed
• [Journal Article] Purification, characterization, and primary structure of a novel N-acyl-D-amino acid amidohydrolase from Microbacterium natoriense TNJL143-2 (2012)
  • Author(s): Liu, J., Asano, Y., Ikoma, K., Yamashita, S., Hirose, Y., Shimoyama, T., Takahashi, S., Nakayama, T. and Nishino, T.
  • Journal Title: J. Biosci. Bioengin Volume: 114 Issue: 4 Pages: 391-397
  • DOI: 10.1016/j.jbiosc.2012.05.015
  • Peer Reviewed
• [Journal Article] Regioselective glucosidation of trans-resveratrol in Escherichia coli expressing glucosyltransferase from Phytolacca americana (2012)
  • Author(s): Shin-ichi Ozaki
  • Journal Title: Biotechnol Lett Volume: 34 Issue: 3 Pages: 475-481
  • DOI: 10.1007/s10529-011-0784-4
  • Peer Reviewed
• [Journal Article] Quantitative analyses of the behavior of exogenously added bacteria during an acidulocomposting process (2012)
  • Author(s): Takatoshi Suematsu
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 114 Issue: 1 Pages: 70-72
  • DOI: 10.1016/j.jbiosc.2012.02.012
  • Peer Reviewed
• [Journal Article] Catalytic removal of acetaldehyde in saliva by a Gluconobacter strain (2012)
  • Author(s): Haruhiko Yamaguchi
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 114 Issue: 3 Pages: 268-274
  • DOI: 10.1016/j.jbiosc.2012.04.008
  • Peer Reviewed
• [Journal Article] 植物フラボノイドグリコシルトランスフェラーゼ：分子系統学と特異性発現機構 (2012)
  • Author(s): 中山 亨
  • Journal Title: 酵素工学ニュース Volume: 67 Pages: 6-10
• [Journal Article] Functional Differentiation of the Glycosyltransferases That Contribute to the Chemical Diversity of Bioactive Flavonol Glycosides in Grapevines (2010)
  • Author(s): Ono, E., Homma, Y., Horikawa, M., Kunikane-Doi, S., Imai, H., Takahashi, S., Kawai, Y., Ishiguro, M., Fukui, Y., Nakayama,T
  • Journal Title: Plant Cell Volume: 22 Pages: 2856-2871
  • Peer Reviewed
• [Journal Article] Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases (AmCHS1) (2010)
  • Author(s): Masayoshi Hatayama, et al
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 107 Pages: 235-239
  • Peer Reviewed
• [Journal Article] Functional Differentiation of the Glycosyltransferases That Contribute to the Chemical Diversity of Bioactive Flavonol Glycosides in Grapevine (Vitis vinifera L.) (2010)
  • Author(s): Eiichiro Ono, et al
  • Journal Title: The Plant Cell Volume: 22 Pages: 2856-2871
  • Peer Reviewed
• [Journal Article] Production of tetraketide lactones by mutated Antirrhinum majus chalcone synthases(AmCHS1) (2010)
  • Author(s): M.Hatayama, et al.
  • Journal Title: Journal of Bioscience and Bioengineering (印刷中)
  • Peer Reviewed
• [Journal Article] Effect of mutagenesis at the region upstream from the G(Q/E) motif of three types of geranylgeranyl diphosphate synthase on product chain-length (2010)
  • Author(s): M.Noike, et al.
  • Journal Title: Journal of Bioscience and Bioengineering 107 Pages: 235-239
  • Peer Reviewed
• [Journal Article] New role of flavin as a general acid-base catalyst with no dedox function in type 2 isopentenyl-diphosphate isomerase (2009)
  • Author(s): Unno, H., Yamashita, S., Ikeda, Y., Sekiguchi, S., Yoshida, N., Yoshimura, T., Kusunoki,M., Nakayama, T. Nishino, T., Hemmi, H.
  • Journal Title: J. Biol. Chem Volume: 284 Issue: 14 Pages: 9160-9167
  • DOI: 10.1074/jbc.m808438200
  • Peer Reviewed
• [Journal Article] Local differentiation of sugar donor speciocity of flavonoid glycosyltransferase in Lamiales (2009)
  • Author(s): Noguchi, A., Horikawa, M., Fukui, Y., Fukuchi-Mizutani, M., Iuchi-Okada, A., Ishiguro, M., Kiso, Y., Nakayama, T., Ono, E.
  • Journal Title: Plant Cell Volume: 21 Pages: 1556-1572
  • Peer Reviewed
• [Journal Article] New Role of Flavine as a General Acid-Base Catalyst with No Redox Function in Type 2 Isopentenyl-Diphosphate Synthase (2009)
  • Author(s): H.Unno, et al.
  • Journal Title: The Journal of Biological Chemistry 284 Pages: 9160-9167
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): K.Yonekura-Sakakibara, et al.
  • Journal Title: Anthocyanins : Biosynthesis, Functions, and Applications.(Springer) Pages: 169-190
• [Presentation] Purification, gene cloning, and biochemical characterization of a β-Glucosidase capable of hydrolyzing sesaminol triglucoside from Paenibacillus sp. Strain KB0549 (2012)
  • Author(s): Nakayama, T
  • Organizer: 6th Japan-Finland Biotechnology Symposium 2012
  • Place of Presentation: 東北学院大学土 樋キャンパス(仙台市)
• [Presentation] ブドウのフラボノール3位グリコシルトランスフェラーゼにおける糖供与体特異性の進化 (2011)
  • Author(s): 中山亨
  • Organizer: 日本応用酵素協会第37回研究発表会
  • Place of Presentation: 大阪
  • Year and Date: 2011-11-14
• [Presentation] ブドウの生理活性フラボノイド配糖体の生合成に関わる糖転移酵素(GT)の機能進化 (2011)
  • Author(s): 中山亨
  • Organizer: 第426回ビタミンB研究協議会
  • Place of Presentation: 大津(ピアザ淡海)
  • Year and Date: 2011-11-05
• [Presentation] キンギョソウにおける花色発現の代謝戦略 (2011)
  • Author(s): 中山亨
  • Organizer: 日本生化学会シンポジウム「植物の高次機能を司る機能性低分子化合物」
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] 多様な生物に見られるユニークな代謝と酵素:機能・進化解析と応用への展望 (2011)
  • Author(s): 中山亨
  • Organizer: ダイズイソフラボン生合成酵素群にみられるユニークな代謝と酵素日本農芸化学会シンポジウム
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2011-03-25
• [Presentation] 黄色フラボノイド オーロンの生合成経路の解明と花色改変技術への利用 (2010)
  • Author(s): 中山亨
  • Organizer: 園芸学会公開シンポジウムIII園芸植物の品質特性(色,香り,生体防御)の代謝生理
  • Place of Presentation: 大分大学
  • Year and Date: 2010-09-19
• [Presentation] 酵素・タンパク質のユニークな活性調節機構と機能発現の新展開 (2010)
  • Author(s): 中山亨
  • Organizer: 植物グリコシルトランスフェラーゼの機能進化.日本農芸化学会シンポジウム
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-03-30
• [Presentation] バイオで開く21世紀の科学 (2009)
  • Author(s): 中山亨
  • Organizer: 黄色い花もいかが?～酵素ハンティングと花色改変～日本化学会東北支部地区講演会
  • Place of Presentation: 山形大学(山形市)
  • Year and Date: 2009-11-26
• [Presentation] Comparative biochemistry of plant glucuronosyltransferase (2009)
  • Author(s): T.Nakayama
  • Organizer: JAPAN-ITALY SYMPOSIUM : New Trends in Enzyme Science and Technology
  • Place of Presentation: ナポリ(イタリア)
  • Year and Date: 2009-10-27
• [Presentation] Comparative biochemistry of plant glucuronosyltransferases. Italy-Japan Sympojium (2009)
  • Author(s): Nakayama, T
  • Organizer: New Trends in Enzyme Science and Technology
  • Place of Presentation: イタリアナポリ
• [Presentation] 植物におけるグルクロノシル化反応の比較生化学第2回博多シンポジウム (2009)
  • Author(s): 中山亨
  • Organizer: 内外環境と生物応答
  • Place of Presentation: 博多
• [Presentation] Comparative studies on biochemistry and phylogenetics of three glucuronosyltransferases involved in anthocyanins andrelated flavonoids (2009)
  • Author(s): Nakayama, T
  • Organizer: 5th International Workshop on Anthocyanins,2009 in Japan
  • Place of Presentation: 名古屋
• [Presentation] ダイズイソフラボン生合成酵素群にみられる ユニークな代謝と酵素
  • Author(s): 中山 亨
  • Organizer: 日本農芸化学会大会シンポジウム
  • Place of Presentation: 京都
  • Invited
• [Presentation] ダイズイソフラボン生合成系酵素群の局在性と 酵素間相互作用
  • Author(s): 中山 亨
  • Organizer: 酵素補酵素研究会
  • Place of Presentation: 名古屋
• [Presentation] Paenibacillus sp. KB0549株の セサミノールトリグルコシド分解酵素 精製，遺伝子クローニング，キャラクタリゼーション
  • Author(s): 中山 亨
  • Organizer: 食品酵素化学研究会 第12回学術講演会
  • Place of Presentation: 盛岡
• [Presentation] Purification, gene cloning, and biochemical characterization of a β-Glucosidase capable of hydrolyzing sesaminol triglucoside from Paenibacillus sp. Strain KB0549
  • Author(s): Toru Nakayama
  • Organizer: 6th Japan-Finland Biotechnology Symposium 2012
  • Place of Presentation: 仙台
  • Invited
• [Presentation] キンギョソウにおけるメタボロンを介する フラボノイド生合成に関する研究
  • Author(s): 中山 亨
  • Organizer: 植物生理学会
  • Place of Presentation: 岡山
• [Presentation] フラボノイドアグリコンの合成・修飾・脱修飾を介する植物の環境応答制御
  • Author(s): 中山 亨
  • Organizer: 日本農芸化学会2013年度（仙台）大会シンポジウム
  • Place of Presentation: 仙台
  • Invited
• [Book] Handbook of Proteolytic Enzymes, 3rd Edition (2013)
  • Author(s): Toru Nakayama
  • Total Pages: 4
  • Publisher: Elsevier
• [Book] ひらく、ひらく「バイオの世界」―14 歳からの生物工学入門 (2012)
  • Author(s): 日本生物工学会（中山 亨）
  • Total Pages: 18
  • Publisher: 化学同人
• [Book] 工学系のための生化学（第６章） (2012)
  • Author(s): 中山 亨
  • Total Pages: 29
  • Publisher: 化学同人
• [Book] 酵素利用技術体系 (2010)
  • Author(s): 中山亨, 他
  • Publisher: エヌティーエス