Molecular dissection of prenyltransferase family involved in flavonoid functionalization and the enzyme engineering
| Project/Area Number |
21310141
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2010: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2009: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | フラボノイド / プレニル化 / 酵素工学 / 膜蛋白質 / プラスチド局在 / プレニル化酵素 / ポリフェノール / 二次代謝 / 生合成 / プラスチド / プレニル基転移酵素 / イソプレノイド / 代謝工学 / クララ / ホップ / 有用物質生産 |
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| Research Abstract |
The first isoflavone-specific prenyltransferase SfG6DT and the first chalcone-specific one SfiLDT have been cloned from cultured cells of Sophora flavescens by utilizing flavone-specific prenyltransferase SfN8DT-1. Both have 9 transmembrane alpha-helices and plastid-targeting signal, and by phylogenetic analysis it was turned out that all those flavonoid prenyltransferase belong to prenyltransferase family for vitamin E biosynthesis. Moreover, a new enzyme GmG4DT involved in phytoalexin biosynthesis in soybean has been identified. Beyond Leguminosae, flavonioid prenyltransferases have been isolated in Macaranga tanarius(Euphorbiaceae), as well. By making serial chimeric enzyme between SfN8DT and SfG6DT, a determinant region for substrate recognition could be determined. Furthermore, metabolic engineering using those enzyme genes have been carried out in plant and bacterial hosts.
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Report
(4 results)
Research Products
(38 results)
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[Journal Article] Molecular cloning and characterization of a cDNA for pterocarpan4-dimethylallyltransferase catalyzing the keyprenylation step in the biosynthesis ofglyceollin, a soybean phytoalexin2009
Author(s)
Akashi, T., Sasaki, K., Aoki, T., Ayabe, S., Yazaki, K.
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Journal Title
PlantPhysiol.
Volume: 149
Issue: 2
Pages: 683-693
DOI
Related Report
Peer Reviewed
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